Difference between revisions of "Os04g0101400"
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| − | + | The rice '''''Os04g0101400''''' was reported as '''''CYP93G1''''' in 2014 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:98-Os04g0101400.png|right|thumb|327px|'''Figure 4.''' ''HPLC-MS/MS metabolite analysis of Arabidopsis tt6 plants overexpressing CYP93G1.<ref name="ref1" />.'']] | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os04g0101400''''' '''''<=>''''' '''''CYP93G1,OsFNSII,FNSII,OsCYP93G1''''' | ||
===Function=== | ===Function=== | ||
| − | + | * '''''CYP93G1''''' is a bona fide flavone synthase II (FNSII) that catalyzes the direct conversion of flavanones to flavones. | |
| + | * In recombinant enzyme assays, '''''isCYP93G1''''' is desaturated naringenin and eriodictyol to apigenin and luteolin, respectively. | ||
| + | * Transgenic expression of '''''CYP93G1''''' in Arabidopsis (Arabidopsis thaliana) resulted in the accumulation of different flavone O-glycosides, which are not naturally present in cruciferous plants. | ||
| + | * '''''CYP93G1''''' is a key branch point enzyme channeling flavanones to the biosynthesis of tricin O-linked conjugates in rice. | ||
| − | === | + | ===Phenotypic analysis=== |
| − | + | * To understand its metabolic roles in planta, the coding sequence of CYP93G1 was placed under the control of the cauliflower mosaic virus 35S promoter and transformed into the Arabidopsis transparent testa6 (tt6) mutant. | |
| − | + | * Transgenic seeds were germinated on medium devoid of nitrogen sources. Under such conditions, the endogenous flavonoid pathway in Arabidopsis is highly active in providing potential substrates for the engineered enzyme. | |
| − | + | * Ten-day-old T1 seedlings were harvested for methanol extraction, followed by acid hydrolysis, which releases flavonoid aglycones from O-linked conjugates. HPLC-MS/MS analysis detected the presence of apigenin, lu- teolin, and chrysoeriol in all of the CYP93G1 transgenic plant samples (Fig. 4A). | |
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You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China | |
| + | * State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | ||
| + | ==References== | ||
| + | <references> | ||
| + | * <ref name="ref1"> | ||
| + | Lam PY, Zhu FY, Chan WL, Liu H, Lo C. Cytochrome P450 93G1 Is a Flavone | ||
| + | Synthase II That Channels Flavanones to the Biosynthesis of Tricin O-Linked | ||
| + | Conjugates in Rice. Plant Physiol. 2014 May 19;165(3):1315-1327. [Epub ahead of | ||
| + | print] PubMed PMID: 24843076; PubMed Central PMCID: PMC4081339. | ||
| + | </ref> | ||
| + | </references> | ||
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==Structured Information== | ==Structured Information== | ||
| − | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]] | |
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| − | [[Category:Genes]] | ||
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| − | [[Category:Oryza Sativa Japonica Group]] | ||
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Latest revision as of 06:54, 17 October 2016
The rice Os04g0101400 was reported as CYP93G1 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 4. HPLC-MS/MS metabolite analysis of Arabidopsis tt6 plants overexpressing CYP93G1.[1].
Gene Symbol
- Os04g0101400 <=> CYP93G1,OsFNSII,FNSII,OsCYP93G1
Function
- CYP93G1 is a bona fide flavone synthase II (FNSII) that catalyzes the direct conversion of flavanones to flavones.
- In recombinant enzyme assays, isCYP93G1 is desaturated naringenin and eriodictyol to apigenin and luteolin, respectively.
- Transgenic expression of CYP93G1 in Arabidopsis (Arabidopsis thaliana) resulted in the accumulation of different flavone O-glycosides, which are not naturally present in cruciferous plants.
- CYP93G1 is a key branch point enzyme channeling flavanones to the biosynthesis of tricin O-linked conjugates in rice.
Phenotypic analysis
- To understand its metabolic roles in planta, the coding sequence of CYP93G1 was placed under the control of the cauliflower mosaic virus 35S promoter and transformed into the Arabidopsis transparent testa6 (tt6) mutant.
- Transgenic seeds were germinated on medium devoid of nitrogen sources. Under such conditions, the endogenous flavonoid pathway in Arabidopsis is highly active in providing potential substrates for the engineered enzyme.
- Ten-day-old T1 seedlings were harvested for methanol extraction, followed by acid hydrolysis, which releases flavonoid aglycones from O-linked conjugates. HPLC-MS/MS analysis detected the presence of apigenin, lu- teolin, and chrysoeriol in all of the CYP93G1 transgenic plant samples (Fig. 4A).
You can also add sub-section(s) at will.
Labs working on this gene
- School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China
- State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
References
- ↑ 1.0 1.1 Lam PY, Zhu FY, Chan WL, Liu H, Lo C. Cytochrome P450 93G1 Is a Flavone Synthase II That Channels Flavanones to the Biosynthesis of Tricin O-Linked Conjugates in Rice. Plant Physiol. 2014 May 19;165(3):1315-1327. [Epub ahead of print] PubMed PMID: 24843076; PubMed Central PMCID: PMC4081339.
