Difference between revisions of "Os06g0102100"
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| − | + | The rice '''''Os06g0102100''''' was reported as '''''CYP93G2''''' in 2010 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:99-Os06g0102100.png|right|thumb|327px|'''Figure 5.''' ''Analysis of the rice CYP93G2 T-DNA mutant<ref name="ref1" />.'']] | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os06g0102100''''' '''''<=>''''' '''''OsF2H,F2H''''','''''CYP93G2''''' | ||
===Function=== | ===Function=== | ||
| − | + | * Rice '''''CYP93G2''''' Is a Close Homolog of Sorghum CYP93G3 | |
| − | + | * '''''CYP93G2''''' is related to the CYP93B subfamily, which consists of dicot flavone synthase II enzymes. | |
| + | * '''''CYP93G2''''' generates 2-hydroxy-flavanones, which are modified by O-glycosylation in transgenic Arabidopsis (Arabidopsis thaliana). | ||
| + | * '''''CYP93G2''''' is the first enzyme that channels flavanones to C-glycosylflavone biosynthesis in rice. | ||
===Expression=== | ===Expression=== | ||
| − | + | * The mutant plants did not show observable differences from the wild-type plants with regard to growth, flowering time, or seed yield. | |
| − | + | Both tricin and isovitexin are frequently O-glycosylated (Cavaliere et al., 2005), and the aglycones can be released by acid treatment. | |
| − | + | * In the acid-hydrolyzed methanolic extracts of '''''CYP93G2''''' mutant leaves, the levels of tricin detected were comparable to those of wild-type samples (Fig. 5C). However, the accumulation of isovitexin was substantially reduced in the mutant plants, suggesting that '''''CYP93G2''''' is required for C-glycosylflavone production in rice. In nonhydrolyzed leaf extracts, a range of potential flavone metabolites were detected in the wild-type samples following HPLC separation, which was monitored at 350 nm (Fig. 5D). | |
| − | + | * On the other hand, the majority of these UV light-absorbing compounds eluted at 15 to 22 min were markedly reduced in absorbance in the mutant chromatogram. | |
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * School of Biological Sciences (Y.D., H.C., C.L.) and Department of Chemistry (I.K.C.), The University of Hong Kong, Hong Kong, China | |
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Du Y, Chu H, Chu IK, Lo C. CYP93G2 is a flavanone 2-hydroxylase required for | ||
| + | C-glycosylflavone biosynthesis in rice. Plant Physiol. 2010 Sep;154(1):324-33. | ||
| + | doi: 10.1104/pp.110.161042. Epub 2010 Jul 20. PubMed PMID: 20647377; PubMed | ||
| + | Central PMCID: PMC2938165. | ||
| + | |||
| + | </ref> | ||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
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[[Category:Genes]] | [[Category:Genes]] | ||
[[Category:Japonica mRNA]] | [[Category:Japonica mRNA]] | ||
Latest revision as of 07:13, 17 October 2016
The rice Os06g0102100 was reported as CYP93G2 in 2010 [1] by researchers from China.
Contents
Annotated Information
Figure 5. Analysis of the rice CYP93G2 T-DNA mutant[1].
Gene Symbol
- Os06g0102100 <=> OsF2H,F2H,CYP93G2
Function
- Rice CYP93G2 Is a Close Homolog of Sorghum CYP93G3
- CYP93G2 is related to the CYP93B subfamily, which consists of dicot flavone synthase II enzymes.
- CYP93G2 generates 2-hydroxy-flavanones, which are modified by O-glycosylation in transgenic Arabidopsis (Arabidopsis thaliana).
- CYP93G2 is the first enzyme that channels flavanones to C-glycosylflavone biosynthesis in rice.
Expression
- The mutant plants did not show observable differences from the wild-type plants with regard to growth, flowering time, or seed yield.
Both tricin and isovitexin are frequently O-glycosylated (Cavaliere et al., 2005), and the aglycones can be released by acid treatment.
- In the acid-hydrolyzed methanolic extracts of CYP93G2 mutant leaves, the levels of tricin detected were comparable to those of wild-type samples (Fig. 5C). However, the accumulation of isovitexin was substantially reduced in the mutant plants, suggesting that CYP93G2 is required for C-glycosylflavone production in rice. In nonhydrolyzed leaf extracts, a range of potential flavone metabolites were detected in the wild-type samples following HPLC separation, which was monitored at 350 nm (Fig. 5D).
- On the other hand, the majority of these UV light-absorbing compounds eluted at 15 to 22 min were markedly reduced in absorbance in the mutant chromatogram.
You can also add sub-section(s) at will.
Labs working on this gene
- School of Biological Sciences (Y.D., H.C., C.L.) and Department of Chemistry (I.K.C.), The University of Hong Kong, Hong Kong, China
