Difference between revisions of "Os03g0726100"

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Please input one-sentence summary here.
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The rice '''''Os03g0726100''''' was reported as '''''OsTubA1''''' and '''''RiP-3''''' in 2000 and 2014, respectively <ref name="ref1" /> <ref name="ref2" />  by researchers from Republic of Korea and Canada.  
  
 
==Annotated Information==
 
==Annotated Information==
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[[File:101-Os03g0726100.png|right|thumb|327px|'''Figure 1.''' ''RNA gel blot showing the expression of RiP-3 gene in various parts of the panicle and the penultimate leaf of 6-week-old well-watered (control) and water-stressed rice plants.<ref name="ref1" />.'']]
 
===Function===
 
===Function===
Please input function information here.
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* '''''RiP-3''''' encodes a putative α-tubulin protein.
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* The expression of α-tubulin gene may be part of the events that suppress panicle elongation during water deficit, which is in turn a suspected cause of male reproductive failure and yield reduction in rice.
  
 
===Expression===
 
===Expression===
Please input expression information here.
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* Expression of α-tubulin cDNA in various reproductive and vegetative organs of 6-week old well-watered and water-stressed rice plants was probed with RiP-3 cDNA. The cDNA hybridized to an expected 1.4 Kb transcript (Fig. 1).
 
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* The highest expression was detected in ovaries, followed by rachis and glumes (lemma þ palea). No expression was detected in the penultimate leaf (the uppermost fully expanded leaf). Water stress suppressed α-tubulin expression in ovaries, rachis and bracts but promoted it in anthers (40%).  
===Evolution===
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* Densitometric analysis showed that the greatest decrease in transcript level under water stress was in glumes (63%), followed by rachis (25%) and ovaries (12%).
Please input evolution information here.
 
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
Please input related labs here.
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* Institut de recherche en biologie végétale, Université de Montréal, Montreal, QC H1X 2B2, Canada
 
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* Department of Life Science and National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790–784, Republic of Korea
 
==References==
 
==References==
Please input cited references here.
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<references>
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* <ref name="ref1">
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Jeon JS, Lee S, Jung KH, Jun SH, Kim C, An G. Tissue-preferential expression
 +
of a rice alpha-tubulin gene, OsTubA1, mediated by the first intron. Plant
 +
Physiol. 2000 Jul;123(3):1005-14. PubMed PMID: 10889249; PubMed Central PMCID:
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PMC59063.
 +
</ref>
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* <ref name="ref2">
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Sheoran IS, Koonjul P, Attieh J, Saini HS. Water-stress-induced inhibition of
 +
α-tubulin gene expression during growth, and its implications for reproductive
 +
success in rice. Plant Physiol Biochem. 2014 Jul;80:291-9. doi:
 +
10.1016/j.plaphy.2014.04.011. Epub 2014 Apr 23. PubMed PMID: 24814750.
 +
</ref>
 +
</references>
  
 
==Structured Information==
 
==Structured Information==
{{JaponicaGene|
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]
GeneName = Os03g0726100|
 
Description = Similar to Tubulin alpha-1 chain (Alpha-1 tubulin)|
 
Version = NM_001057667.1 GI:115455062 GeneID:4333966|
 
Length = 2884 bp|
 
Definition = Oryza sativa Japonica Group Os03g0726100, complete gene.|
 
Source = Oryza sativa Japonica Group
 
 
 
  ORGANISM  Oryza sativa Japonica Group
 
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
            clade; Ehrhartoideae; Oryzeae; Oryza.
 
|
 
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
 
AP = Chromosome 3:30287946..30290829|
 
CDS = 30288071..30288163,30289110..30289344,30289431..30289801,30289910..30290566|
 
GCID = <gbrowseImage1>
 
name=NC_008396:30287946..30290829
 
source=RiceChromosome03
 
preset=GeneLocation
 
</gbrowseImage1>|
 
GSID = <gbrowseImage2>
 
name=NC_008396:30287946..30290829
 
source=RiceChromosome03
 
preset=GeneLocation
 
</gbrowseImage2>|
 
CDNA = <cdnaseq>atgagggagtgcatctcgatccacatcggccaggccggtatccaggtcgggaacgcgtgctgggagctctactgcctcgagcatggcatccaggctgatggtcagatgcccagtgacaggactgttggtggaggtgatgatgctttcaacaccttcttcagtgagactggtgctgggaagcatgttccccgtgctgtatttgttgatcttgagcctactgtgattgatgaggtgaggactggttgctaccgccagctcttccaccctgagcagctcatcaatggcaaggaggatgcagctaacaactttgcccgtggtcactacaccattggcaaggagattgttgatctgtgccttgaccgcatcaggaagcttgctgacaactgcactggtctccaaggtttccttgtgttcaacgctgttggaggtggaacgggctctggtcttggttcccttctcctggagcgcctttctgtggactatggcaagaagtccaagcttgggttcactgtgtacccatcccctcaggtctccacctctgtggttgagccatacaacagtgtcctctctacccactccctccttgagcacactgatgttgctgttctgcttgataatgaggccatctatgacatctgccgccgctcccttgacattgagcgcccaacctacaccaacctcaacaggcttgtgtctcaggttatctcatccctgaccgcctccctgaggtttgatggtgctctgaacgtggatgtcaatgagttccagaccaaccttgtgccctacccaaggatccacttcatgctttcgtcctacgctccagtgatctctgcggagaaggcctaccatgagcaactctctgttgccgagatcaccaacagtgccttcgagccatcctccatgatggccaagtgcgaccctcgccacggcaagtacatggcctgctgcctcatgtaccgcggcgatgtcgtgcccaaggacgtgaacgccgccgtcgccaccatcaagaccaagcgcaccatccagttcgtggactggtgcccgacggggttcaagtgcggcatcaactaccagccgcccagcgtcgtccccagcggcgacctcgccaaggtgcagagggccgtgtgcatgatctccaactccaccagcgtcgtggaggtgttctcccgcatcgaccacaagttcgacctcatgtactccaagcgcgccttcgtccactggtacgtgggtgagggcatggaggagggtgagttctctgaggcccgtgaggatctcgccgcgttggagaaggactacgaggaggttggcgctgagtccgacgagaatgaggatggcgatgatggtgacgagtactag</cdnaseq>|
 
AA = <aaseq>MRECISIHIGQAGIQVGNACWELYCLEHGIQADGQMPSDRTVGG                    GDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGCYRQLFHPEQLINGKEDAANN                    FARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVD                    YGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLD                    IERPTYTNLNRLVSQVISSLTASLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVI                    SAEKAYHEQLSVAEITNSAFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVAT                    IKTKRTIQFVDWCPTGFKCGINYQPPSVVPSGDLAKVQRAVCMISNSTSVVEVFSRID                    HKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESDENEDGDDGDE                    Y</aaseq>|
 
DNA = <dnaseqindica>126..218#1165..1399#1486..1856#1965..2621#aagccgagacaacgcagagaaaggcgtcttcgtactcgcctctctccgcgcctccgcgcttttcctcctcctctcccctctctcccttctccgccgccgtcgcagcatcaacccaatccgccgccatgagggagtgcatctcgatccacatcggccaggccggtatccaggtcgggaacgcgtgctgggagctctactgcctcgagcatggcatccaggtacggatccgcgtcccatctccctcaccccccgtgttcttcgtgcctgcttctgggtcagatctgggtggattcgcggttgttggatgtggggggctgtgtttatttgtcggtggatctggttgtctggatctgcgttttctctgtcgtagttagcggatctgatgaaatgtttagtgttcgtgtatactggtatggtggatctggtcctaggatgcgtggaatggatatatgtaggcgaattggaggatttattttgtgaattttgctgaaatgatagttctaaacactggatctgacctcgggatgctgttaaatgtggaaatcatggtcgatgctgtcatgaacatggtgttcttatggtagatctgagcaatgtatgtttcaaaattgtttgtcacatggaaatgctatggttctagatgcaatagaatgatacatgccgagatcccctctagttgatatgatagatcatgatgttttacagctatgtcatatgaatatgttcatttgttaccgatgtatttggatctacttaacatttccaaagcacgccgcgttctaattctagatctggtagtcatgtttgtacacgtcacccacctaatacaaatacatatgtctagtgtttggtgacactgcccgtcagatctgttttttccagatctgtggaacaaatactccacgcatgtatggtagttttgaaacgatcttgtatcttccattgttgtagtaacaactaaataaagtacaattgttcaattattgggaatcgtattttctgtagtgccgatgtacagcatattcatagatgtctatttaggaactcaaattttaaattgaggactagttatttattgtgggtcagtcttttgaattgtgttatcttgctgtactgaaataataatgtaccactaaggcgctaacatgtattttgtcctttcaggctgatggtcagatgcccagtgacaggactgttggtggaggtgatgatgctttcaacaccttcttcagtgagactggtgctgggaagcatgttccccgtgctgtatttgttgatcttgagcctactgtgattgatgaggtgaggactggttgctaccgccagctcttccaccctgagcagctcatcaatggcaaggaggatgcagctaacaactttgcccgtggtcactacaccagtgagttccattcttactaccacggtgctattttttttgttatgtggctaattacatgactaacttggggtgctaaatctttacagttggcaaggagattgttgatctgtgccttgaccgcatcaggaagcttgctgacaactgcactggtctccaaggtttccttgtgttcaacgctgttggaggtggaacgggctctggtcttggttcccttctcctggagcgcctttctgtggactatggcaagaagtccaagcttgggttcactgtgtacccatcccctcaggtctccacctctgtggttgagccatacaacagtgtcctctctacccactccctccttgagcacactgatgttgctgttctgcttgataatgaggccatctatgacatctgccgccgctcccttgacattgagcgcccaacctacaccaacctcaacaggcttgtgtctcaggtacagtcttgtatccttgcaatgatctcaaattctctgctatgttaattcactgccatttcttgtttgtgctgttctctaaccaacttgtttattgctaatgtgcaggttatctcatccctgaccgcctccctgaggtttgatggtgctctgaacgtggatgtcaatgagttccagaccaaccttgtgccctacccaaggatccacttcatgctttcgtcctacgctccagtgatctctgcggagaaggcctaccatgagcaactctctgttgccgagatcaccaacagtgccttcgagccatcctccatgatggccaagtgcgaccctcgccacggcaagtacatggcctgctgcctcatgtaccgcggcgatgtcgtgcccaaggacgtgaacgccgccgtcgccaccatcaagaccaagcgcaccatccagttcgtggactggtgcccgacggggttcaagtgcggcatcaactaccagccgcccagcgtcgtccccagcggcgacctcgccaaggtgcagagggccgtgtgcatgatctccaactccaccagcgtcgtggaggtgttctcccgcatcgaccacaagttcgacctcatgtactccaagcgcgccttcgtccactggtacgtgggtgagggcatggaggagggtgagttctctgaggcccgtgaggatctcgccgcgttggagaaggactacgaggaggttggcgctgagtccgacgagaatgaggatggcgatgatggtgacgagtactagaggagtcgtcgtcgtctgggggcttgatgttctgtgtgtcaaggcctgattgataactgctgctatcccatgatctgccagtgtggagttatcctgttgccgtgtgcgtgtgtcttcgagacatttgcttgtggtgatctgatgtttggggcttaatgggcttacaaccctcgttgttgtaacctgtgtgccctgttttatgtgagaccgcttcgtcacttataatatgcgcgtttgttcaattttatgtttgtttcttgtgt</dnaseqindica>|
 
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057667.1 RefSeq:Os03g0726100]|
 
}}
 
[[Category:Genes]]
 
[[Category:Japonica mRNA]]
 
[[Category:Oryza Sativa Japonica Group]]
 
[[Category:Japonica Genes]]
 
[[Category:Japonica Chromosome 3]]
 
[[Category:Chromosome 3]]
 

Latest revision as of 12:11, 17 October 2016

The rice Os03g0726100 was reported as OsTubA1 and RiP-3 in 2000 and 2014, respectively [1] [2] by researchers from Republic of Korea and Canada.

Annotated Information

Figure 1. RNA gel blot showing the expression of RiP-3 gene in various parts of the panicle and the penultimate leaf of 6-week-old well-watered (control) and water-stressed rice plants.[1].

Function

  • RiP-3 encodes a putative α-tubulin protein.
  • The expression of α-tubulin gene may be part of the events that suppress panicle elongation during water deficit, which is in turn a suspected cause of male reproductive failure and yield reduction in rice.

Expression

  • Expression of α-tubulin cDNA in various reproductive and vegetative organs of 6-week old well-watered and water-stressed rice plants was probed with RiP-3 cDNA. The cDNA hybridized to an expected 1.4 Kb transcript (Fig. 1).
  • The highest expression was detected in ovaries, followed by rachis and glumes (lemma þ palea). No expression was detected in the penultimate leaf (the uppermost fully expanded leaf). Water stress suppressed α-tubulin expression in ovaries, rachis and bracts but promoted it in anthers (40%).
  • Densitometric analysis showed that the greatest decrease in transcript level under water stress was in glumes (63%), followed by rachis (25%) and ovaries (12%).

You can also add sub-section(s) at will.

Labs working on this gene

  • Institut de recherche en biologie végétale, Université de Montréal, Montreal, QC H1X 2B2, Canada
  • Department of Life Science and National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang 790–784, Republic of Korea

References

  1. 1.0 1.1 Jeon JS, Lee S, Jung KH, Jun SH, Kim C, An G. Tissue-preferential expression of a rice alpha-tubulin gene, OsTubA1, mediated by the first intron. Plant Physiol. 2000 Jul;123(3):1005-14. PubMed PMID: 10889249; PubMed Central PMCID: PMC59063.
  2. Sheoran IS, Koonjul P, Attieh J, Saini HS. Water-stress-induced inhibition of α-tubulin gene expression during growth, and its implications for reproductive success in rice. Plant Physiol Biochem. 2014 Jul;80:291-9. doi: 10.1016/j.plaphy.2014.04.011. Epub 2014 Apr 23. PubMed PMID: 24814750.

Structured Information