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| − | Please input one-sentence summary here.
| + | The rice '''''Os05g0154600''''' was reported as '''''APIP6''''' in 2012 <ref name="ref1" /> by researchers from China. |
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| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:107-Os05g0154600.png|right|thumb|327px|'''Figure 2.''' ''Response of AvrPiz-t Transgenic Rice to Blast Infection, flg22, and Chitin Treatments.<ref name="ref1" />.'']] |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * '''''APIP6''''' in is a Functional RING Finger E3 Ubiquitin Ligase |
| − | | + | * AvrPiz-t suppresses the ubiquitin ligase activity of the rice RING E3 ubiquitin ligase '''''APIP6''''' and that, in return, APIP6 ubiquitinates AvrPiz-t in vitro. |
| | + | * Agroinfection assays reveal that AvrPiz-t and AvrPiz-t Interacting Protein 6 ('''''APIP6''''') are both degraded when coexpressed in Nicotiana benthamiana. |
| | + | * Silencing of '''''APIP6''''' in transgenic rice leads to a significant reduction of flg22-induced ROS generation, suppression of defense-related gene expression, and enhanced susceptibility of rice plants to M. oryzae. |
| | ===Expression=== | | ===Expression=== |
| − | Please input expression information here.
| + | * In both the T1 (primary transformants) and T2 generations, the RNAi plants grew normally and did not show any changes in growth and development. |
| − | | + | * Blast inoculations showed that the '''''APIP6''''' RNAi plants were as resistant as the wild-type Piz-t plants (TRD) to the isolate, suggesting that APIP6 is not required for the Piz-t-mediated resistance to the avirulent strain. |
| − | ===Evolution===
| + | * Punch inoculation assays showed that lesions were larger on the '''''APIP6''''' RNAi plants than on the segregated wild-type plants (Figure 7A). |
| − | Please input evolution information here.
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * Department of Plant Pathology, Ohio State University, Columbus, OH 43210 |
| | + | * State Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
| | + | * Biotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China |
| | + | * State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021 China |
| | + | * Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506 |
| | + | * Department of Horticulture and Crop Science, Ohio State University, Columbus, OH 43210 |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| | + | * <ref name="ref1"> |
| | + | Park, Chan-Ho, et al. "The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 Ubiquitin Ligase APIP6 to suppress pathogen-associated molecular pattern–triggered immunity in rice." The Plant Cell 24.11 (2012): 4748-4762. |
| | + | </ref> |
| | + | </references> |
| | + | |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 5]] |
| − | GeneName = Os05g0154600|
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| − | Description = Similar to VIP2 protein|
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| − | Version = NM_001061218.1 GI:115462166 GeneID:4337844|
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| − | Length = 5703 bp|
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| − | Definition = Oryza sativa Japonica Group Os05g0154600, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 5|Chromosome 5]]|
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| − | AP = Chromosome 5:3167898..3173600|
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| − | CDS = 3168121..3168586,3169097..3169505,3169588..3169616,3170657..3170715,3171578..3171747<br>,3173422..3173599|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008398:3167898..3173600
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| − | source=RiceChromosome05
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008398:3167898..3173600
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| − | source=RiceChromosome05
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>agggaggaggtgagggaggaggagggggaggagggggagggggtgggcgggaaggaggaggagaaggcggcggccgcggccgcggcggtgtcgtgctcgatctgcctcgacgcggttgtcgccgccggcggggagaggtccacggcgaggctgcagtgtggccacgagttccacctcgattgcattggatcagcatttaatgccaaaggcgttatgcaatgccccaactgccgcaaaattgaaaaggggaactggctctatgcaaatggttcccgacccacacaagatgttaatatggatgagtgggctcatgatgaagacctttatgatgttagttactcggagatgccctttcggtttcactggtgcccatttggtcgtttggctcaacttccatccttctttgaggaaggagaatcatcacccccagttacttttcatgacttcatgggacaacatatgtttacggagcatgtagctgctgtatcttctgcacctggggcagctcatccatgcccatatgtggcatacttgcatcctcttccatcattggcgtcatcatcaagctctcatgttcctgagagaactatggatggtcctgcatatcatgatccctggcaccccttggctgggccttcagatggtaggcccttgcaatcagtgcaacctgctgattttcatcataaccattgggcgcatgttcccaattcctatcctcaacccaacaacaataatggggtggccgagcagcaaggagtcccttttggaacaacgagggctgcaagagttgatggtgatacccaacgtcgaggatcatctatttctccatcatacttcagtaatggatctggttctagatctagagctcctaatgttcctcctatggtacctcaattcatgagggcgcacggcagcattagtgaacagtaccagcagagttcatcatccagcttatttgctggggctcatagatcaggaggcatgcgaactgcaccgccgccccctctaccagagaacccggcattttgccttttcccaccaggttcatctggccataactcgatggagactgatgatgcaggaggaaaccgattctatgcctgggaacgggatcgcttcgcaccatacccactaatgccagtggactgtgagacaaactggtggagttcacaacaatctcatggtacatcagaacccgcgcctgctccaaggagactatttgggcaatggattggtgttggaagatcatctccggaaaatcgatcacccgagggctcatcgtatcggcaaatgcacaccccaaggatgtag</cdnaseq>|
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| − | AA = <aaseq>REEVREEEGEEGEGVGGKEEEKAAAAAAAVSCSICLDAVVAAGG ERSTARLQCGHEFHLDCIGSAFNAKGVMQCPNCRKIEKGNWLYANGSRPTQDVNMDEW AHDEDLYDVSYSEMPFRFHWCPFGRLAQLPSFFEEGESSPPVTFHDFMGQHMFTEHVA AVSSAPGAAHPCPYVAYLHPLPSLASSSSSHVPERTMDGPAYHDPWHPLAGPSDGRPL QSVQPADFHHNHWAHVPNSYPQPNNNNGVAEQQGVPFGTTRAARVDGDTQRRGSSISP SYFSNGSGSRSRAPNVPPMVPQFMRAHGSISEQYQQSSSSSLFAGAHRSGGMRTAPPP PLPENPAFCLFPPGSSGHNSMETDDAGGNRFYAWERDRFAPYPLMPVDCETNWWSSQQ SHGTSEPAPAPRRLFGQWIGVGRSSPENRSPEGSSYRQMHTPRM</aaseq>|
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| − | DNA = <dnaseqindica>5015..5480#4096..4504#3985..4013#2886..2944#1854..2023#2..179#gagggaggaggtgagggaggaggagggggaggagggggagggggtgggcgggaaggaggaggagaaggcggcggccgcggccgcggcggtgtcgtgctcgatctgcctcgacgcggttgtcgccgccggcggggagaggtccacggcgaggctgcagtgtggccacgagttccacctcggtatgtatgtggctttcggatgatctacagatgtgttattggtagggaattgtaagttagagcactagaactgcgattttttttggactcgcgctgtcaatttgatttgggtgagggtagctgtaaaatgtgatgcttttgttaggaattggggctggacttggtgggaattaagataattttacgtcttctagctgtaatggtgcagaattttgggcgatggttcgcataggtagtgagtgccttttgggggtaaccatcaatgttttccccagtgggtgggtgggttgggggatgaaaggaagatttcgtgctaaaattgctggtatatagggtgaaatagtctggctgacatgcatcacaaatttccggtactggcctggtccgatgactcgtagagtttgcggatttgatcagatactatgaaagaacatcagaatagcttgagcggagtttcttgatgggttcttagttcaatggaagttatgaaacaaagcatcggaccaaataattattttagccagacaaggaaatcaagtaaatcacatcagttggaaattgatactaacatggaaaccaatggaagcaaacagatagagcaaatattgatcttgagattgcgcttctctataccatggtagaggcaggttggcatgttgagcagataataaattggccccttaatacaacgatggacctatgcacccactggtaacctttaaacagggctggcttagtggaccaatggtttgacccacccatttggacccctggatgaaagtctgtttggttcccaggaatttttgggggttattgagctagctgttcaattttagtgaatggttgcactttgaaggcttgtctggaggatttaaaaatgtcccatggcatgcttagacctaaacttataggaaactaaggacgacacagatttgaatagttcagtttgatggtttcattgagcgttgagccatagtgacactgacatactgtagtagatagggtttccctctctcctttttatgctgtacaaggaattaggtgcaatcctgagaatatgtagttaactgcaatccttgtttcctactttcctggaaattactcttaggtaatattttttgttggaagtcagaaccatgtttacattctatatatacttggttcatgttcttttactggtatcaagttttatctggtctgttgttatttggtaacaagttatcttgatgggattgagtttttgtcttataagctgtcagccgtgacgcatgagcaaggcaacagaaaaataattttagtgcagacttctatacttctactcttagtggttaaaagcctaaggctgaaaaatagagaaggatggaaaaacatctaaactaattcaaaattaagtttcaaaactcaaatttggccgcagcttataagctgccgagctgccaacaagttagtatcaaatatggactgtcttttagttatcaaaatcctttagattttctaagttaacaagcctcaatgatcttggattcacttattaaccaccattgttttttgtgcattagcaatgattttatttgctttttacatttgttaagcgtatgatcttcaagtggaagttttgaatttctagtattttcatttattacaacattttgatgattgtggcattgtttccagattgcattggatcagcatttaatgccaaaggcgttatgcaatgccccaactgccgcaaaattgaaaaggggaactggctctatgcaaatggttcccgacccacacaagatgttaatatggatgagtgggctcatgatgaagacctttatgatgttagttactcggagatggtacgcaaccatgttatctttaattttttatttgtttttgttgtgaattatctttttcctttctaatctttaatttattatttgtttttgttgtgaattgtcttttacctttcttgtttgccacatgctatcccatcatgtttttactagtttacctgatcatcaatatggtttcttcgacttgttcttttcttacaaatgattcacttgactaaaccttttcttttggagacatctgtgttgctacgtgaatatgtcttctatgccgatattcccttcagatcaagatctttagttatgggcctcttgacagcggaattcatttataatcgttcttataggttgccactggcagcttttgtgaattgcattactgttgtttttctgttctccaatttagtcacttgaggccatgttctctctgtgaagaaaacaatatgggagttttgaacaagtgcagtgtgcattcttgcaaatgatctagattctagttcttttgtcagttgaaggttaaactcgagagaagtaatacattagaggtcacagctgtttttttatgtgtacacactatggtgcatcagtactgactccttatttacttgaagtaaatttgtcattttccctattattatgcttaacattgtgcacaaagtaataattctgagatgaaagttgtcacagcttattcaatcatgctacacatatgcactaactaattgcattttctaaaagatttatgctacttacatagttggttttcattattattgatttttcattttctgtcatcatttgagcttacggaggcagttactctagattctgttctgagttctgacaacaatgtatttgaattgtgcagccctttcggtttcactggtgcccatttggtcgtttggctcaacttccatccttctttgagtatgttctgcagtcttcgcttgaaattgtggtttatcgttttatttcacctttccaattgtccagttctatttctagctcagcactataagatctttcaagtatagtaataatataaaacaccattgaatttgcgggtagtgaaaattggtcttatgaggtactataactagatgttgtatttatgttggtccattagcgatgctatctatactctatactcctcaaaaagattccaatggtggtggcattgaatctgccaccccacccactccttttgtagattttacatattaccccttgaacttttaatgttacataacaactaaatctaggtggatagccacatatacaattaaattaaggtgggtattccctataacaaaaagacgggtaacatatacatttcatgaaaaatatgttattgcttattttaggcaatattatgaaaatattcttttatctatccatagcaaagcatatgggtatttagctagtgttttgaaaaattaataagcaaatgagttaactctaggacctgtaatatcaacaatctgttttagggagggatgaatataaggtgaatggcctttcaattctcggtgatgaagtgcggaacaatatacattttttcttaggtttagtcctatcatgatgcatttggtttttgacaatctccgtgctagttctctttccatgagactctaactctactttgcatggatcttctgacaagccactgtctattgcttgtatataccaatatgtcagtataccatttgaccaaagtgctgatcagactactggtgacagcattaatgatgataccattaagccatattgtctttcccgataccgattgcagtcagtgaacatatcaattctttaagttctatcttttgctgcacccttctcttaacaccaactattgtctgtgcaaaatctatagcacgctatattaaaagtttcagagtttcattgtttgaaattggtgctatgtaatattatgttcctcacttaatgttcccctcttttgccagggaaggagaatcatcacccccagttacttgtaagttcttatttaaatgggatcattttgtaaaacaatagctatattgagtttgaaatatgtcttgatattggccatgcagttcatgacttcatgggacaacatatgtttacggagcatgtagctgctgtatcttctgcacctggggcagctcatccatgcccatatgtggcatacttgcatcctcttccatcattggcgtcatcatcaagctctcatgttcctgagagaactatggatggtcctgcatatcatgatccctggcaccccttggctgggccttcagatggtaggcccttgcaatcagtgcaacctgctgattttcatcataaccattgggcgcatgttcccaattcctatcctcaacccaacaacaataatggggtggccgagcagcaaggagtcccttttggaacaacgagggctgcaagagttgatggtgatacccaacgtcgaggatcatctatttctccatcatacttcagtaatgggtaattacttattcatttctgctcaatctactaatttgtccttgtttttgttgccatgtactttatcagaacacatatcagattagtaaaatcagtaaacaatggaaaattgtgttgggtaccaaaaaagacattgtacgcatccgtgtcctattctgctaccatttttaccaatactgtttgtccacgtttcctttgctggttttatcatgctctgttttaacctgatgctataacaactttcacaaatacaattttgtatttttgtttatgctataacaaaaaaaatgggaacctgatggtgaaatcaataataagcttgtaagcatgagtgtcaggctaagatcttatacaggcaagaataaattcctaaaattacttcaaattcaaaatcttcttttgtagaagagactattgcattattatgcagcttctgattttcataaaaatgcagcttctgattcttcaatgacgaatatgtagttatgactaccttttttttttttgcagatctggttctagatctagagctcctaatgttcctcctatggtacctcaattcatgagggcgcacggcagcattagtgaacagtaccagcagagttcatcatccagcttatttgctggggctcatagatcaggaggcatgcgaactgcaccgccgccccctctaccagagaacccggcattttgccttttcccaccaggttcatctggccataactcgatggagactgatgatgcaggaggaaaccgattctatgcctgggaacgggatcgcttcgcaccatacccactaatgccagtggactgtgagacaaactggtggagttcacaacaatctcatggtacatcagaacccgcgcctgctccaaggagactatttgggcaatggattggtgttggaagatcatctccggaaaatcgatcacccgagggctcatcgtatcggcaaatgcacaccccaaggatgtagaccctcatgaagggaatttaagttaatacttaacatttgaaatctgaagttgcttgtaagaagatggaaaacccattgtatgaaattaagcccgggtcgtgccgtaagcctcgaagcaaatctgagaagaagaaacagaaaataacccggggtggacattttgatttggtttattgagttccatcacgtacatatttgaaatcaagttgggatattcggagtt</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001061218.1 RefSeq:Os05g0154600]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 5]]
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| − | [[Category:Chromosome 5]]
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