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| − | Please input one-sentence summary here.
| + | The rice '''''Os07g0592200''''' was reported as '''''OsAP65''''' in 2013 <ref name="ref1" /> by researchers from China. |
| | | | |
| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:129-Os07g0592200.png|right|thumb|327px|'''Figure 2.''' ''Pollen grains from OsAP65 T-DNA insertional mutant and wild-type plants.<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os07g0592200''''' '''''<=>''''' '''''OsAP65,AP65''''' |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * '''''OsAP65''''' encodes a rice aspartic protease |
| − | | + | * '''''OsAP65''''' is essential for male fertility and plays a role in pollen germination and pollen tube growth. |
| − | ===Expression=== | + | ===Phenotypic analysis=== |
| − | Please input expression information here.
| + | * The result showed that the total number of pollen grains in an anther and the percentage of mature pollen of '''''OsAP65'''''+/– plants were not different from those of '''''OsAP65'''''+/+ plants. |
| − | | + | * DAPI staining showed that all pollen grains from both OsAP65+/– and OsAP65+/+ plants contained three nuclei in the mature pollen: two bright, intensely stained sperm nuclei and one diffuse, weakly stained vegetative nucleus (Fig. 2A, B). |
| − | ===Evolution===
| + | * This analysis indicated that the mutation did not affect sperm cell development and division. SEM was used to examine the surface of the pollen grains, and no significant difference in pollen morphology could be detected between the '''''OsAP65'''''+/– and '''''OsAP65'''''+/+ plants (Fig. 2C, D, F, G). |
| − | Please input evolution information here.
| + | * TEM scanning of pollen grains at maturity did not reveal subcellular changes between the OsAP65+/– and OsAP65+/+ plants (Fig. 2E, H). This also implies that disruption of '''''OsAP65''''' does not cause a visible difference in pollen morphology. |
| | + | * Pollen germination and pollen tube elongation were then examined in vitro and in vivo. The percentage of germinated pollen grains in vitro was found to be significantly lower in '''''OsAP65'''''+/– plants (56.78%) than in '''''OsAP65'''''+/+ plants (79.64%) (Fig. 2I, J, L). |
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| | + | * <ref name="ref1"> |
| | + | Huang J, Zhao X, Cheng K, Jiang Y, Ouyang Y, Xu C, Li X, Xiao J, Zhang Q. |
| | + | OsAP65, a rice aspartic protease, is essential for male fertility and plays a |
| | + | role in pollen germination and pollen tube growth. J Exp Bot. 2013 |
| | + | Aug;64(11):3351-60. doi: 10.1093/jxb/ert173. PubMed PMID: 23918968; PubMed |
| | + | Central PMCID: PMC3733154. |
| | + | </ref> |
| | + | </references> |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 7]] |
| − | GeneName = Os07g0592200|
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| − | Description = Peptidase A1, pepsin family protein|
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| − | Version = NM_001066696.1 GI:115473124 GeneID:4343783|
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| − | Length = 7606 bp|
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| − | Definition = Oryza sativa Japonica Group Os07g0592200, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
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| − | AP = Chromosome 7:24789873..24797478|
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| − | CDS = 24790243..24790414,24790906..24791003,24791089..24791190,24791359..24791480,24792796..24792972<br>,24793968..24794040,24794160..24794256,24794337..24794419,24794780..24794936<br>,24795060..24795487,24796917..24797037,24797169..24797434|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008400:24789873..24797478
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| − | source=RiceChromosome07
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008400:24789873..24797478
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| − | source=RiceChromosome07
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atggcgagtcgccggtcgcgtcggctcggctcgctcgtgcccttcgccgccgcggtggtcctcctcctcctcatcgcttccgccgccgccgccgccgcgggatcccccgatcgggccggccccgcgcggccgccgctggtgctcccgctcacgctcgcctaccccaacgccacccgcctccccgcctcctcggccaggcgcggcctcggcgatggacacaaccccaacgcccgcatgcgcctccacgatgatctcctcaccaacgggtattacacgacgaggctgtacatcgggacgccttcgcaggagttcgcgctgatcgtggactccgggagcaccgtcacctacgttccctgcgccacctgcgagcagtgcggcaaccaccaggatccacgattccaacctgatctctccagtacatattcaccagtaaaatgcaatgttgattgtacttgtgataatgagaggagtcaatgcacttatgagaggcagtatgctgaaatgagctccagcagtggtgtgcttggcgaggacattatgtcatttggcaaagaaagtgaacttaagccacagcgtgctgtttttggctgtgaaaacaccgaaacaggagatttgttcagtcaacatgctgatggtattatggggctgggccgtggtcaacttagcataatggatcagcttgttgagaagggtgtcattagtgattcattctctctgtgctatggtggaatggatgttggtggtggtactatggtacttggtgggatgcctgctcctcctgacatggttttctctcattcaaaccctgtccgcagcccgtattacaacattgagttaaaggaaatacatgttgccggaaaggcattgcggttagacccaaagatattcaacagcaaacatggcactgtcttagatagtggaaccacatatgcttacctgcctgaacaagcatttgttgcatttaaagatgctgtgacaaataaagtaaattctctgaagaaaatccgtggcccagatccaaattataaagacatctgctttgcaggtgctggaaggaacgtctcccaattatcagaggtatttccagatgttgacatggtatttggaaatgggcagaagctttcgctttcacctgaaaactatttgttccggcattcgaaggttgaaggggcttattgcttgggtgtgtttcagaatggaaaagatccgacaacactattagggggaattgttgtccgtaatactcttgtaacctatgaccgtcacaatgagaagattggcttttggaaaactaactgttcagagttgtgggagaggctccatattagtgaagttccttcatcagcaccttcagattcagagggagatatggcaccagctcctgctcctagtggtttgcctgaatttgatgttggcctaatcactgttgatatgtccataaatgttacttatccaaacctgaaacctcacctccatgagctggctgagcttatagctaaagagctggacattgactcccgtcaggttcgagttatgaatgttacaagccaaggaaactctactctaatcagatgggggatttttccagctggaccttctaattctatgactaatacaacagcaatgggtatcatttatcggttgacccaacatcacgtccagttgcctgaaaatcttggcagttatcagttacttgaatggaatgtgcagcctttatccaagaggtcatggttccgagatcatgtagtctctatactgcttggaattttgctagttgttttgctgactttatcggcccttttagtactgattgtctggagaaagaaatttagaggtcaagcggcatatagacctgtggattcagcagttcctgagcaagagctccaaccgctataa</cdnaseq>|
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| − | AA = <aaseq>MASRRSRRLGSLVPFAAAVVLLLLIASAAAAAAGSPDRAGPARP PLVLPLTLAYPNATRLPASSARRGLGDGHNPNARMRLHDDLLTNGYYTTRLYIGTPSQ EFALIVDSGSTVTYVPCATCEQCGNHQDPRFQPDLSSTYSPVKCNVDCTCDNERSQCT YERQYAEMSSSSGVLGEDIMSFGKESELKPQRAVFGCENTETGDLFSQHADGIMGLGR GQLSIMDQLVEKGVISDSFSLCYGGMDVGGGTMVLGGMPAPPDMVFSHSNPVRSPYYN IELKEIHVAGKALRLDPKIFNSKHGTVLDSGTTYAYLPEQAFVAFKDAVTNKVNSLKK IRGPDPNYKDICFAGAGRNVSQLSEVFPDVDMVFGNGQKLSLSPENYLFRHSKVEGAY CLGVFQNGKDPTTLLGGIVVRNTLVTYDRHNEKIGFWKTNCSELWERLHISEVPSSAP SDSEGDMAPAPAPSGLPEFDVGLITVDMSINVTYPNLKPHLHELAELIAKELDIDSRQ VRVMNVTSQGNSTLIRWGIFPAGPSNSMTNTTAMGIIYRLTQHHVQLPENLGSYQLLE WNVQPLSKRSWFRDHVVSILLGILLVVLLTLSALLVLIVWRKKFRGQAAYRPVDSAVP EQELQPL</aaseq>|
| |
| − | DNA = <dnaseqindica>7065..7236#6476..6573#6289..6390#5999..6120#4507..4683#3439..3511#3223..3319#3060..3142#2543..2699#1992..2419#442..562#45..310#ccccttccttcctcctcctcggctcggcgcgacgagtcctcgccatggcgagtcgccggtcgcgtcggctcggctcgctcgtgcccttcgccgccgcggtggtcctcctcctcctcatcgcttccgccgccgccgccgccgcgggatcccccgatcgggccggccccgcgcggccgccgctggtgctcccgctcacgctcgcctaccccaacgccacccgcctccccgcctcctcggccaggcgcggcctcggcgatggacacaaccccaacgcccgcatgcgcctccacgatgatctcctcaccaacgggtcggtacagttcgttttttgccctccgctctctctctctcgataatttcccccaaatttttttttcttgggggggttgcgtggttggatctgaggctggatgtcgccgcgtgttgttcgtgctggtttaggtattacacgacgaggctgtacatcgggacgccttcgcaggagttcgcgctgatcgtggactccgggagcaccgtcacctacgttccctgcgccacctgcgagcagtgcggcaaccaccaggtcggcctggattcgtcaaattctctgcttacctgtttgaattgtgtgtgacgatcggtttaattaggattggtgatggtcgcgtgtatggatattggattcccatatgtcaaactgtcgcttgaccattcaggttggacaattgactccagtgcaaatgatacatttttgtcgggcttgggtgtgtgcatagacatttattgttccaaaattcaaagtcttggaaaatacacgtttccatttggataggttcggaagttccagctatacgatgtggataaagtgtatggtacgcaaagagcacctccacaacctgcagggtatattaaaactaaccgagcgacgatgatgtagcttctttttttctcaattgtgaatgcctcagaatttaatagaggtcaacatccaacaaagcccatgccaatcttggtcacaaccaacaagaattactatttgtttgatagccatcattttagccaaattttgcaccattttgttttcaaaccattggcgttcctggttgtcaacagttagtcgattcttctttgttttttctccatgccaaatgagtcccatgtctttcctgagtgctgacaattatcaggcaatagatttgggaagtgtattggattgtttatggcaaagcagtggaaacaatttgtattaacaccaattttcctaccaagtaaacccactgccatgtccagaatacacatctggtattgctatgcaatttgatgtttgttgctcccttatcgttctctgaattttgggctttagattcaaacgtcacatagaaaacatatccatatgaaaaggcaaatatgttactgtatttttcacttctttctgctacatacccagccttttcttgggcaactgtttgcatggttagtcaggccactgttctcttgtagtcctgtataatctgagtagtttggaaatcttccatcagttgtagattgctactattgctgataagtaatagaggagtccacaactaatgggaagcccataacctgattctaaaaagaactgttcttgtcacttgcatagttgcatctttttgacacaattgtatcattcttatcataacttgtgatttttctagattctagatggcaccattgttgtaccatcctcttattctatgtcatgtttgcagagtgaatcacctaacatcattgaggcacatgtaaaagtttaagcatttatactatttaatttatattaatgtgtgtacaaacagtgtgacaatattttggcatgatttctccaatgcgatgtatatcttacgtgataaaaaaagttaagatgtgtatttgatcatacccattgagaaaatcacctttttcccacttgaagtttcctttttaactatctagaaatcaactttctctagtatacactaaagtttggcatgttgtaggatccacgattccaacctgatctctccagtacatattcaccagtaaaatgcaatgttgattgtacttgtgataatgagaggagtcaatgcacttatgagaggcagtatgctgaaatgagctccagcagtggtgtgcttggcgaggacattatgtcatttggcaaagaaagtgaacttaagccacagcgtgctgtttttggctgtgaaaacaccgaaacaggagatttgttcagtcaacatgctgatggtattatggggctgggccgtggtcaacttagcataatggatcagcttgttgagaagggtgtcattagtgattcattctctctgtgctatggtggaatggatgttggtggtggtactatggtacttggtgggatgcctgctcctcctgacatggttttctctcattcaaaccctgtccgcaggtattatacatttttgttttttccacaccagactgaagattcttttagtagcctgctggaggaaggttttctaatgttctgtatatttaatacatttccttattaattttggattgtcaacagcccgtattacaacattgagttaaaggaaatacatgttgccggaaaggcattgcggttagacccaaagatattcaacagcaaacatggcactgtcttagatagtggaaccacatatgcttacctgcctgaacaagcatttgttgcatttaaagatgctgtaagttatcaaatgatatctcctccacttttaccttttttgaattgcttttaccatctgctaactatgctactttactattttatatttaatatatttccctagatgccaattttgcaattaacctgtctagtttcagatgttaagacagaaaaatttactcctactttggcaacacctggttttgcatgtgtacgagccaatctgtttggcaccttttctgctatcttggttgtcgcattgcagattcttttcattttgtttttattcagctcttcattgtcattcctgaaaatggaaatctttaaaatggacactcctggaaaagtatgctggaataattatgtttttaatggataggtgacaaataaagtaaattctctgaagaaaatccgtggcccagatccaaattataaagacatctgctttgcaggtgctggaaggtaactcttgcaccatgataacaatttctaccccctttgttttcatcatcaatgttcttatcttatcactttaccttcaggaacgtctcccaattatcagaggtatttccagatgttgacatggtatttggaaatgggcagaagctttcgctttcacctgaaaactatttgttccgggtatgagaaattccaagcttagacctgttctgatgaattatgacaccccattacttttttgctttgcctgttgttgagttcatgttttgtcttctcttctttccggtttcaaaataaagcattcgaaggttgaaggggcttattgcttgggtgtgtttcagaatggaaaagatccgacaacactattagggggtaagatcttcaactcttggtctcttacgaggaacttcaagaaaatttccattggacttcttttatttatatcatctgactacactatctctactttattcgcatataacttgttactagtgtatgctgaataacatttgcacctgtactatgagtctttgaccagcttatacactcgcgtctgctggaaacttgagtagaaaaccaagtaactctctttgtagaaaatactactatataacaatggtctccttatctttttcagcataaccagctgatgcacttttcaatattttttagatataatctgaggtaacttttgtgtcagtgtagcttgttccttcatcactatggcaatttcttggctgcacttattcaatttcgtaatccaatagtttacgtgtgcaagtatgcaatgattggtgtgtaatccgaaaaccatggctgtgaaataccaacattgtttatcattttatgaatataagaatgcataaaccctctatttttttcatagataacaaacatgcaattatctgtgaacaatccaaaaacaagcatccttaagagttattcctgatttcagaaaatttgaggttagaagcaaagtgttcatggaattatcagttaaaactctaaaagtggttgagcctacaaaggaggcattctctttattaattgccggttgcaagccaacatacggtattgtaccaagaaattttttttctgccaacagtataatagaccttggctgcttgttgtatttatgcactcttttgtctctaaaaggaaagtatggtgtttttgttctcttctctatcccttaaagagtcaagcagatcaatctttagagagatttatacttttcttatattaattttgaccttttatttgatttatagacagtctacatgaatgttctaatagtttgcatggctctatgtactttgaaaccttgcccgctttgagtgtctaaagaataatgatcttactggcaggaattgttgtccgtaatactcttgtaacctatgaccgtcacaatgagaagattggcttttggaaaactaactgttcagagttgtgggagaggctccatattagtgaagttccttcatcagcaccttcagattcagagggagatatggcaccagctcctgctcctagtggtttgcctggtacagccctacctatacaactattttgttaaaactattgatttgctgcaactttagcttgtcattttagagaactgtggcaataaggtagaattttatcagaaagctgcaactttgactggcaagttgggcccacatgtcatatgcacatagggggataaagttgcagttttctaataaaattgtttgctattgttgcagttttgtgaaacactatgcttaagttgtagaaaagttatagtattgaccaaattattgcacttttctgaaatttactctctatctttcttcattttggtggtcatgttttatgaacgttgaatcacaagcatttgctatttttataatttcgaatggtcaactattatcatcttctttggggtactttctttggatgttatccagaagtttcattgggatcaaatttttctttgaaatgggtgcagacagcccaatctttccccattttttatctgcaccatttcatttttgtttacctaatttttttctcaaataattttgtttacctacttaattaaattacctagaaatcacctagttattttgttttggtagctcttgcctaataacttaaatcattatttttatttagtgcacactattatagatggccaaaaggcccgaggcccgacggcccggcccatggcacggcgtttaggcccggcccaagcacggcacggcccgtcaagggtcgggcccgtgccggcccggcccgaccaccgggccgtgcctgggcctctccgctggcacgttgggccggcacggcacaatgggccgcatgccaagcccgacacataaacaatagggagtaaaaatagacatattatatgccacggtcgaaagaatagggatgtaaaatagacttattttacctatatatatgtcagcccaaagaggagggacggaaaatagacttattttcactatatatatgtcacagcccaaagtggagggacggaaaatagacttattttcgatatattatgtcacagcccaaagaggagggacgaaaaatagacttatttaaaaaaaggcacgatgggctacctgcgccttcgggccggtccggcacagcccgacggttggtgggccgtgcctaggcgggaggcgcagcccatgggccgtgccggcccggcacgaagtgccgatcgggccgtgccggcccgatggctggtgggccgtgcctggctgtgcctgggccaggccgtgccgggcgggccttttggccatctatacacactatttctagctttattttatgcgctgatgaataaggtttttctcctgcttgcagaatttgatgttggcctaatcactgttgatatgtccataaatgttacttatccaaacctgaaacctcacctccatgagctggctgagcttatagctaaagagctggacattgactcccgtcaggtaagggtgacccatagtctgtgctgttgtggtttttctagttttgtttggaggtgatgcgtgagggacactatccttgctattgtttacagctttgcaccatcctatcttgatagcactagcattcttttgaagtgatttgttaaagttgcctcttgagtttgacaggttcgagttatgaatgttacaagccaaggaaactctactctaatcagatgggggatttttccagctggaccttctaattctatgactaatacaacagcaatggtagcatccactcttgcacatatgaggactactttaatctatctgttataaagataattgatttgctgcattgatgtatgattagggtatcatttatcggttgacccaacatcacgtccagttgcctgaaaatcttggcagttatcagttacttgaatggaatgtgcagcctttatccaagaggtaagcaaacaccattataactgccaagattatattgccattaaaagcttgtacagtccatattatcataataagctctgtctttatcattggaattcaactctttcttttcattgaagccctgctttcatcctgatctaaaatgacaaggcaacatcccttcgacatcctttttgcagggaaaaagtagatacatgtcactctacactagcctgtttagagatataccattataaactataaagttctctctcccaaacactattcttatactgatcagtggctttagcgtgtagttgtagtatgcaatggggctgcctggcaaacatggaaaacctgaatatctgtcaaagtgcttaagcaagattctcacatctattaggaaaaatcattttcaccaattcccttgaatatttcttgtgcggcatcgatgcatcaatcttggcattttcatctgctggagaacttacattcggtaattatttctacaggtcatggttccgagatcatgtagtctctatactgcttggaattttgctagttgttttgctgactttatcggcccttttagtactgattgtctggagaaagaaatttagaggtcaagcggcatatagacctgtggattcagcagttcctgagcaagagctccaaccgctataatttggagtggttcaacggagtaaacatataggcgattctttggtagaaataccgttgtgcgggctgccatgttgcagcgggttcacatccgctgctgggagttgccctttttctttgtatgtaacacaagctacaagcttacagaagaagcacaagattacataacttctcgattcagcacttagagcctctgttcataaactgtctagcgattcagatcaagagcttcctcttgtagcttacagtagaagaaggttttgttcgtgtaatgtagattaagtagaatagagcaaggcacaaaagcacagcagccatggacggaatgttgtattatctagtatctgataagtgctgaggatattcggcttct</dnaseqindica>|
| |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001066696.1 RefSeq:Os07g0592200]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 7]]
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| − | [[Category:Chromosome 7]]
| |
