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| − | {{JaponicaGene|
| + | The rice '''''Os07g0592200''''' was reported as '''''OsAP65''''' in 2013 <ref name="ref1" /> by researchers from China. |
| − | GeneName = Os07g0592200|
| |
| − | Description = Peptidase A1, pepsin family protein|
| |
| − | Version = NM_001066696.1 GI:115473124 GeneID:4343783|
| |
| − | Length = 7606 bp|
| |
| − | Definition = Oryza sativa Japonica Group Os07g0592200, complete gene.|
| |
| − | Source = Oryza sativa Japonica Group
| |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| + | ==Annotated Information== |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| + | [[File:129-Os07g0592200.png|right|thumb|327px|'''Figure 2.''' ''Pollen grains from OsAP65 T-DNA insertional mutant and wild-type plants.<ref name="ref1" />.'']] |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| + | ===Gene Symbol=== |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| + | *'''''Os07g0592200''''' '''''<=>''''' '''''OsAP65,AP65''''' |
| − | |
| + | ===Function=== |
| − | Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
| + | * '''''OsAP65''''' encodes a rice aspartic protease |
| − | AP = Chromosome 7:24789873..24797478|
| + | * '''''OsAP65''''' is essential for male fertility and plays a role in pollen germination and pollen tube growth. |
| − | CDS = 24790243..24790414,24790906..24791003,24791089..24791190,24791359..24791480,24792796..24792972<br>,24793968..24794040,24794160..24794256,24794337..24794419,24794780..24794936<br>,24795060..24795487,24796917..24797037,24797169..24797434|
| + | ===Phenotypic analysis=== |
| − | GCID = <gbrowseImage1>
| + | * The result showed that the total number of pollen grains in an anther and the percentage of mature pollen of '''''OsAP65'''''+/– plants were not different from those of '''''OsAP65'''''+/+ plants. |
| − | name=NC_008400:24789873..24797478
| + | * DAPI staining showed that all pollen grains from both OsAP65+/– and OsAP65+/+ plants contained three nuclei in the mature pollen: two bright, intensely stained sperm nuclei and one diffuse, weakly stained vegetative nucleus (Fig. 2A, B). |
| − | source=RiceChromosome07
| + | * This analysis indicated that the mutation did not affect sperm cell development and division. SEM was used to examine the surface of the pollen grains, and no significant difference in pollen morphology could be detected between the '''''OsAP65'''''+/– and '''''OsAP65'''''+/+ plants (Fig. 2C, D, F, G). |
| − | preset=GeneLocation
| + | * TEM scanning of pollen grains at maturity did not reveal subcellular changes between the OsAP65+/– and OsAP65+/+ plants (Fig. 2E, H). This also implies that disruption of '''''OsAP65''''' does not cause a visible difference in pollen morphology. |
| − | </gbrowseImage1>|
| + | * Pollen germination and pollen tube elongation were then examined in vitro and in vivo. The percentage of germinated pollen grains in vitro was found to be significantly lower in '''''OsAP65'''''+/– plants (56.78%) than in '''''OsAP65'''''+/+ plants (79.64%) (Fig. 2I, J, L). |
| − | GSID = <gbrowseImage2>
| + | |
| − | name=NC_008400:24789873..24797478
| + | You can also add sub-section(s) at will. |
| − | source=RiceChromosome07
| + | |
| − | preset=GeneLocation
| + | ==Labs working on this gene== |
| − | </gbrowseImage2>| | + | * National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China |
| − | CDNA = <cdnaseq>atggcgagtcgccggtcgcgtcggctcggctcgctcgtgcccttcgccgccgcggtggtcctcctcctcctcatcgcttccgccgccgccgccgccgcgggatcccccgatcgggccggccccgcgcggccgccgctggtgctcccgctcacgctcgcctaccccaacgccacccgcctccccgcctcctcggccaggcgcggcctcggcgatggacacaaccccaacgcccgcatgcgcctccacgatgatctcctcaccaacgggtattacacgacgaggctgtacatcgggacgccttcgcaggagttcgcgctgatcgtggactccgggagcaccgtcacctacgttccctgcgccacctgcgagcagtgcggcaaccaccaggatccacgattccaacctgatctctccagtacatattcaccagtaaaatgcaatgttgattgtacttgtgataatgagaggagtcaatgcacttatgagaggcagtatgctgaaatgagctccagcagtggtgtgcttggcgaggacattatgtcatttggcaaagaaagtgaacttaagccacagcgtgctgtttttggctgtgaaaacaccgaaacaggagatttgttcagtcaacatgctgatggtattatggggctgggccgtggtcaacttagcataatggatcagcttgttgagaagggtgtcattagtgattcattctctctgtgctatggtggaatggatgttggtggtggtactatggtacttggtgggatgcctgctcctcctgacatggttttctctcattcaaaccctgtccgcagcccgtattacaacattgagttaaaggaaatacatgttgccggaaaggcattgcggttagacccaaagatattcaacagcaaacatggcactgtcttagatagtggaaccacatatgcttacctgcctgaacaagcatttgttgcatttaaagatgctgtgacaaataaagtaaattctctgaagaaaatccgtggcccagatccaaattataaagacatctgctttgcaggtgctggaaggaacgtctcccaattatcagaggtatttccagatgttgacatggtatttggaaatgggcagaagctttcgctttcacctgaaaactatttgttccggcattcgaaggttgaaggggcttattgcttgggtgtgtttcagaatggaaaagatccgacaacactattagggggaattgttgtccgtaatactcttgtaacctatgaccgtcacaatgagaagattggcttttggaaaactaactgttcagagttgtgggagaggctccatattagtgaagttccttcatcagcaccttcagattcagagggagatatggcaccagctcctgctcctagtggtttgcctgaatttgatgttggcctaatcactgttgatatgtccataaatgttacttatccaaacctgaaacctcacctccatgagctggctgagcttatagctaaagagctggacattgactcccgtcaggttcgagttatgaatgttacaagccaaggaaactctactctaatcagatgggggatttttccagctggaccttctaattctatgactaatacaacagcaatgggtatcatttatcggttgacccaacatcacgtccagttgcctgaaaatcttggcagttatcagttacttgaatggaatgtgcagcctttatccaagaggtcatggttccgagatcatgtagtctctatactgcttggaattttgctagttgttttgctgactttatcggcccttttagtactgattgtctggagaaagaaatttagaggtcaagcggcatatagacctgtggattcagcagttcctgagcaagagctccaaccgctataa</cdnaseq>|
| + | |
| − | AA = <aaseq>MASRRSRRLGSLVPFAAAVVLLLLIASAAAAAAGSPDRAGPARP PLVLPLTLAYPNATRLPASSARRGLGDGHNPNARMRLHDDLLTNGYYTTRLYIGTPSQ EFALIVDSGSTVTYVPCATCEQCGNHQDPRFQPDLSSTYSPVKCNVDCTCDNERSQCT YERQYAEMSSSSGVLGEDIMSFGKESELKPQRAVFGCENTETGDLFSQHADGIMGLGR GQLSIMDQLVEKGVISDSFSLCYGGMDVGGGTMVLGGMPAPPDMVFSHSNPVRSPYYN IELKEIHVAGKALRLDPKIFNSKHGTVLDSGTTYAYLPEQAFVAFKDAVTNKVNSLKK IRGPDPNYKDICFAGAGRNVSQLSEVFPDVDMVFGNGQKLSLSPENYLFRHSKVEGAY CLGVFQNGKDPTTLLGGIVVRNTLVTYDRHNEKIGFWKTNCSELWERLHISEVPSSAP SDSEGDMAPAPAPSGLPEFDVGLITVDMSINVTYPNLKPHLHELAELIAKELDIDSRQ VRVMNVTSQGNSTLIRWGIFPAGPSNSMTNTTAMGIIYRLTQHHVQLPENLGSYQLLE WNVQPLSKRSWFRDHVVSILLGILLVVLLTLSALLVLIVWRKKFRGQAAYRPVDSAVP EQELQPL</aaseq>|
| + | ==References== |
| − | DNA = <dnaseqindica>7065..7236#6476..6573#6289..6390#5999..6120#4507..4683#3439..3511#3223..3319#3060..3142#2543..2699#1992..2419#442..562#45..310#ccccttccttcctcctcctcggctcggcgcgacgagtcctcgccatggcgagtcgccggtcgcgtcggctcggctcgctcgtgcccttcgccgccgcggtggtcctcctcctcctcatcgcttccgccgccgccgccgccgcgggatcccccgatcgggccggccccgcgcggccgccgctggtgctcccgctcacgctcgcctaccccaacgccacccgcctccccgcctcctcggccaggcgcggcctcggcgatggacacaaccccaacgcccgcatgcgcctccacgatgatctcctcaccaacgggtcggtacagttcgttttttgccctccgctctctctctctcgataatttcccccaaatttttttttcttgggggggttgcgtggttggatctgaggctggatgtcgccgcgtgttgttcgtgctggtttaggtattacacgacgaggctgtacatcgggacgccttcgcaggagttcgcgctgatcgtggactccgggagcaccgtcacctacgttccctgcgccacctgcgagcagtgcggcaaccaccaggtcggcctggattcgtcaaattctctgcttacctgtttgaattgtgtgtgacgatcggtttaattaggattggtgatggtcgcgtgtatggatattggattcccatatgtcaaactgtcgcttgaccattcaggttggacaattgactccagtgcaaatgatacatttttgtcgggcttgggtgtgtgcatagacatttattgttccaaaattcaaagtcttggaaaatacacgtttccatttggataggttcggaagttccagctatacgatgtggataaagtgtatggtacgcaaagagcacctccacaacctgcagggtatattaaaactaaccgagcgacgatgatgtagcttctttttttctcaattgtgaatgcctcagaatttaatagaggtcaacatccaacaaagcccatgccaatcttggtcacaaccaacaagaattactatttgtttgatagccatcattttagccaaattttgcaccattttgttttcaaaccattggcgttcctggttgtcaacagttagtcgattcttctttgttttttctccatgccaaatgagtcccatgtctttcctgagtgctgacaattatcaggcaatagatttgggaagtgtattggattgtttatggcaaagcagtggaaacaatttgtattaacaccaattttcctaccaagtaaacccactgccatgtccagaatacacatctggtattgctatgcaatttgatgtttgttgctcccttatcgttctctgaattttgggctttagattcaaacgtcacatagaaaacatatccatatgaaaaggcaaatatgttactgtatttttcacttctttctgctacatacccagccttttcttgggcaactgtttgcatggttagtcaggccactgttctcttgtagtcctgtataatctgagtagtttggaaatcttccatcagttgtagattgctactattgctgataagtaatagaggagtccacaactaatgggaagcccataacctgattctaaaaagaactgttcttgtcacttgcatagttgcatctttttgacacaattgtatcattcttatcataacttgtgatttttctagattctagatggcaccattgttgtaccatcctcttattctatgtcatgtttgcagagtgaatcacctaacatcattgaggcacatgtaaaagtttaagcatttatactatttaatttatattaatgtgtgtacaaacagtgtgacaatattttggcatgatttctccaatgcgatgtatatcttacgtgataaaaaaagttaagatgtgtatttgatcatacccattgagaaaatcacctttttcccacttgaagtttcctttttaactatctagaaatcaactttctctagtatacactaaagtttggcatgttgtaggatccacgattccaacctgatctctccagtacatattcaccagtaaaatgcaatgttgattgtacttgtgataatgagaggagtcaatgcacttatgagaggcagtatgctgaaatgagctccagcagtggtgtgcttggcgaggacattatgtcatttggcaaagaaagtgaacttaagccacagcgtgctgtttttggctgtgaaaacaccgaaacaggagatttgttcagtcaacatgctgatggtattatggggctgggccgtggtcaacttagcataatggatcagcttgttgagaagggtgtcattagtgattcattctctctgtgctatggtggaatggatgttggtggtggtactatggtacttggtgggatgcctgctcctcctgacatggttttctctcattcaaaccctgtccgcaggtattatacatttttgttttttccacaccagactgaagattcttttagtagcctgctggaggaaggttttctaatgttctgtatatttaatacatttccttattaattttggattgtcaacagcccgtattacaacattgagttaaaggaaatacatgttgccggaaaggcattgcggttagacccaaagatattcaacagcaaacatggcactgtcttagatagtggaaccacatatgcttacctgcctgaacaagcatttgttgcatttaaagatgctgtaagttatcaaatgatatctcctccacttttaccttttttgaattgcttttaccatctgctaactatgctactttactattttatatttaatatatttccctagatgccaattttgcaattaacctgtctagtttcagatgttaagacagaaaaatttactcctactttggcaacacctggttttgcatgtgtacgagccaatctgtttggcaccttttctgctatcttggttgtcgcattgcagattcttttcattttgtttttattcagctcttcattgtcattcctgaaaatggaaatctttaaaatggacactcctggaaaagtatgctggaataattatgtttttaatggataggtgacaaataaagtaaattctctgaagaaaatccgtggcccagatccaaattataaagacatctgctttgcaggtgctggaaggtaactcttgcaccatgataacaatttctaccccctttgttttcatcatcaatgttcttatcttatcactttaccttcaggaacgtctcccaattatcagaggtatttccagatgttgacatggtatttggaaatgggcagaagctttcgctttcacctgaaaactatttgttccgggtatgagaaattccaagcttagacctgttctgatgaattatgacaccccattacttttttgctttgcctgttgttgagttcatgttttgtcttctcttctttccggtttcaaaataaagcattcgaaggttgaaggggcttattgcttgggtgtgtttcagaatggaaaagatccgacaacactattagggggtaagatcttcaactcttggtctcttacgaggaacttcaagaaaatttccattggacttcttttatttatatcatctgactacactatctctactttattcgcatataacttgttactagtgtatgctgaataacatttgcacctgtactatgagtctttgaccagcttatacactcgcgtctgctggaaacttgagtagaaaaccaagtaactctctttgtagaaaatactactatataacaatggtctccttatctttttcagcataaccagctgatgcacttttcaatattttttagatataatctgaggtaacttttgtgtcagtgtagcttgttccttcatcactatggcaatttcttggctgcacttattcaatttcgtaatccaatagtttacgtgtgcaagtatgcaatgattggtgtgtaatccgaaaaccatggctgtgaaataccaacattgtttatcattttatgaatataagaatgcataaaccctctatttttttcatagataacaaacatgcaattatctgtgaacaatccaaaaacaagcatccttaagagttattcctgatttcagaaaatttgaggttagaagcaaagtgttcatggaattatcagttaaaactctaaaagtggttgagcctacaaaggaggcattctctttattaattgccggttgcaagccaacatacggtattgtaccaagaaattttttttctgccaacagtataatagaccttggctgcttgttgtatttatgcactcttttgtctctaaaaggaaagtatggtgtttttgttctcttctctatcccttaaagagtcaagcagatcaatctttagagagatttatacttttcttatattaattttgaccttttatttgatttatagacagtctacatgaatgttctaatagtttgcatggctctatgtactttgaaaccttgcccgctttgagtgtctaaagaataatgatcttactggcaggaattgttgtccgtaatactcttgtaacctatgaccgtcacaatgagaagattggcttttggaaaactaactgttcagagttgtgggagaggctccatattagtgaagttccttcatcagcaccttcagattcagagggagatatggcaccagctcctgctcctagtggtttgcctggtacagccctacctatacaactattttgttaaaactattgatttgctgcaactttagcttgtcattttagagaactgtggcaataaggtagaattttatcagaaagctgcaactttgactggcaagttgggcccacatgtcatatgcacatagggggataaagttgcagttttctaataaaattgtttgctattgttgcagttttgtgaaacactatgcttaagttgtagaaaagttatagtattgaccaaattattgcacttttctgaaatttactctctatctttcttcattttggtggtcatgttttatgaacgttgaatcacaagcatttgctatttttataatttcgaatggtcaactattatcatcttctttggggtactttctttggatgttatccagaagtttcattgggatcaaatttttctttgaaatgggtgcagacagcccaatctttccccattttttatctgcaccatttcatttttgtttacctaatttttttctcaaataattttgtttacctacttaattaaattacctagaaatcacctagttattttgttttggtagctcttgcctaataacttaaatcattatttttatttagtgcacactattatagatggccaaaaggcccgaggcccgacggcccggcccatggcacggcgtttaggcccggcccaagcacggcacggcccgtcaagggtcgggcccgtgccggcccggcccgaccaccgggccgtgcctgggcctctccgctggcacgttgggccggcacggcacaatgggccgcatgccaagcccgacacataaacaatagggagtaaaaatagacatattatatgccacggtcgaaagaatagggatgtaaaatagacttattttacctatatatatgtcagcccaaagaggagggacggaaaatagacttattttcactatatatatgtcacagcccaaagtggagggacggaaaatagacttattttcgatatattatgtcacagcccaaagaggagggacgaaaaatagacttatttaaaaaaaggcacgatgggctacctgcgccttcgggccggtccggcacagcccgacggttggtgggccgtgcctaggcgggaggcgcagcccatgggccgtgccggcccggcacgaagtgccgatcgggccgtgccggcccgatggctggtgggccgtgcctggctgtgcctgggccaggccgtgccgggcgggccttttggccatctatacacactatttctagctttattttatgcgctgatgaataaggtttttctcctgcttgcagaatttgatgttggcctaatcactgttgatatgtccataaatgttacttatccaaacctgaaacctcacctccatgagctggctgagcttatagctaaagagctggacattgactcccgtcaggtaagggtgacccatagtctgtgctgttgtggtttttctagttttgtttggaggtgatgcgtgagggacactatccttgctattgtttacagctttgcaccatcctatcttgatagcactagcattcttttgaagtgatttgttaaagttgcctcttgagtttgacaggttcgagttatgaatgttacaagccaaggaaactctactctaatcagatgggggatttttccagctggaccttctaattctatgactaatacaacagcaatggtagcatccactcttgcacatatgaggactactttaatctatctgttataaagataattgatttgctgcattgatgtatgattagggtatcatttatcggttgacccaacatcacgtccagttgcctgaaaatcttggcagttatcagttacttgaatggaatgtgcagcctttatccaagaggtaagcaaacaccattataactgccaagattatattgccattaaaagcttgtacagtccatattatcataataagctctgtctttatcattggaattcaactctttcttttcattgaagccctgctttcatcctgatctaaaatgacaaggcaacatcccttcgacatcctttttgcagggaaaaagtagatacatgtcactctacactagcctgtttagagatataccattataaactataaagttctctctcccaaacactattcttatactgatcagtggctttagcgtgtagttgtagtatgcaatggggctgcctggcaaacatggaaaacctgaatatctgtcaaagtgcttaagcaagattctcacatctattaggaaaaatcattttcaccaattcccttgaatatttcttgtgcggcatcgatgcatcaatcttggcattttcatctgctggagaacttacattcggtaattatttctacaggtcatggttccgagatcatgtagtctctatactgcttggaattttgctagttgttttgctgactttatcggcccttttagtactgattgtctggagaaagaaatttagaggtcaagcggcatatagacctgtggattcagcagttcctgagcaagagctccaaccgctataatttggagtggttcaacggagtaaacatataggcgattctttggtagaaataccgttgtgcgggctgccatgttgcagcgggttcacatccgctgctgggagttgccctttttctttgtatgtaacacaagctacaagcttacagaagaagcacaagattacataacttctcgattcagcacttagagcctctgttcataaactgtctagcgattcagatcaagagcttcctcttgtagcttacagtagaagaaggttttgttcgtgtaatgtagattaagtagaatagagcaaggcacaaaagcacagcagccatggacggaatgttgtattatctagtatctgataagtgctgaggatattcggcttct</dnaseqindica>|
| + | <references> |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001066696.1 RefSeq:Os07g0592200]|
| + | * <ref name="ref1"> |
| − | }}
| + | Huang J, Zhao X, Cheng K, Jiang Y, Ouyang Y, Xu C, Li X, Xiao J, Zhang Q. |
| − | [[Category:Genes]] | + | OsAP65, a rice aspartic protease, is essential for male fertility and plays a |
| − | [[Category:Japonica mRNA]]
| + | role in pollen germination and pollen tube growth. J Exp Bot. 2013 |
| − | [[Category:Oryza Sativa Japonica Group]] | + | Aug;64(11):3351-60. doi: 10.1093/jxb/ert173. PubMed PMID: 23918968; PubMed |
| − | [[Category:Japonica Genes]]
| + | Central PMCID: PMC3733154. |
| − | [[Category:Japonica Chromosome 7]] | + | </ref> |
| − | [[Category:Chromosome 7]]
| + | </references> |
| | + | |
| | + | ==Structured Information== |
| | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 7]] |
