Difference between revisions of "Os05g0420300"
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| − | + | The rice '''''Os05g0420300''''' was reported as '''''SERF1''''' in 2013 <ref name="ref1" /> by researchers from China. | |
| − | |||
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:137-Os05g0420300.png|right|thumb|227px|'''Figure 2.''' ''SERF1 Is Specifically Induced by Salt Stress and H2O2 Treatment and Is Expressed in the Vascular Tissues of Roots and Leaves..<ref name="ref1" />.'']] | ||
===Function=== | ===Function=== | ||
| − | + | * '''''ERF1''''' Regulates Reactive Oxygen Species–Dependent Signaling during the Initial Response to Salt Stress in Rice | |
| − | + | * Loss of '''''SERF1''''' impairs the salt-inducible expression of genes encoding members of a mitogen-activated protein kinase (MAPK) cascade and salt tolerance–mediating TFs. | |
| + | * '''''SERF1''''' is a phosphorylation target of MAPK5, resulting in enhanced transcriptional activity of '''''SERF1''''' toward its direct target genes. | ||
| + | * '''''SERF1''''' amplifies the reactive oxygen species–activated MAPK cascade signal during the initial phase of salt stress and translates the salt-induced signal into an appropriate expressional response resulting in salt tolerance. | ||
===Expression=== | ===Expression=== | ||
| − | + | * Application of ABA or mannitol did not affect the expression of SERF1. | |
| + | * By contrast, as observed for salt stress, H2O2 treatment caused an upregulation of SERF1 within 30 min exclusively in roots, albeit an induction was also observed after 3 h of treatment. | ||
| + | * Rice plants expressing a b-glucuronidase (GUS) reporter gene fused to a 1-kb promoter of SERF1 exhibited GUS activity in the vascular cylinder of the root and the main and commissural veins of leaves (Figure 2B). | ||
| − | === | + | ===Subcellular localization=== |
| − | + | * Although SERF1 does not contain a known nuclear localization signal, stable transformation of rice plants with a construct encoding a CFP-tagged version of SERF1 revealed nuclear localization of SERF1 in leaf epidermal cells (Figure 2C). | |
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany | |
| + | * Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany | ||
| + | * Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Unité Mixte de Recherche, Genetic Improvement and Adaptation of Mediterranean and Tropical Plants, 34398 Montpellier, cedex 5, France | ||
| + | * Department of Molecular Genetics, Centre for Research in Agricultural Genomics, Consejo Superior de Investigaciones Científicos, Institute of Agro-food Research and Technology, Autonomus University of Barcelona, University of Barcelona, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain | ||
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Schmidt R, Mieulet D, Hubberten HM, Obata T, Hoefgen R, Fernie AR, Fisahn J, | ||
| + | San Segundo B, Guiderdoni E, Schippers JH, Mueller-Roeber B. Salt-responsive ERF1 | ||
| + | regulates reactive oxygen species-dependent signaling during the initial response | ||
| + | to salt stress in rice. Plant Cell. 2013 Jun;25(6):2115-31. doi: | ||
| + | 10.1105/tpc.113.113068. Epub 2013 Jun 25. PubMed PMID: 23800963; PubMed Central | ||
| + | PMCID: PMC3723616. | ||
| + | </ref> | ||
| + | </references> | ||
| + | |||
==Structured Information== | ==Structured Information== | ||
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 05]] | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 05]] | ||
Latest revision as of 07:29, 20 October 2016
The rice Os05g0420300 was reported as SERF1 in 2013 [1] by researchers from China.
Contents
Annotated Information
Figure 2. SERF1 Is Specifically Induced by Salt Stress and H2O2 Treatment and Is Expressed in the Vascular Tissues of Roots and Leaves..[1].
Function
- ERF1 Regulates Reactive Oxygen Species–Dependent Signaling during the Initial Response to Salt Stress in Rice
- Loss of SERF1 impairs the salt-inducible expression of genes encoding members of a mitogen-activated protein kinase (MAPK) cascade and salt tolerance–mediating TFs.
- SERF1 is a phosphorylation target of MAPK5, resulting in enhanced transcriptional activity of SERF1 toward its direct target genes.
- SERF1 amplifies the reactive oxygen species–activated MAPK cascade signal during the initial phase of salt stress and translates the salt-induced signal into an appropriate expressional response resulting in salt tolerance.
Expression
- Application of ABA or mannitol did not affect the expression of SERF1.
- By contrast, as observed for salt stress, H2O2 treatment caused an upregulation of SERF1 within 30 min exclusively in roots, albeit an induction was also observed after 3 h of treatment.
- Rice plants expressing a b-glucuronidase (GUS) reporter gene fused to a 1-kb promoter of SERF1 exhibited GUS activity in the vascular cylinder of the root and the main and commissural veins of leaves (Figure 2B).
Subcellular localization
- Although SERF1 does not contain a known nuclear localization signal, stable transformation of rice plants with a construct encoding a CFP-tagged version of SERF1 revealed nuclear localization of SERF1 in leaf epidermal cells (Figure 2C).
You can also add sub-section(s) at will.
Labs working on this gene
- Institute of Biochemistry and Biology, University of Potsdam, 14476 Potsdam, Germany
- Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Unité Mixte de Recherche, Genetic Improvement and Adaptation of Mediterranean and Tropical Plants, 34398 Montpellier, cedex 5, France
- Department of Molecular Genetics, Centre for Research in Agricultural Genomics, Consejo Superior de Investigaciones Científicos, Institute of Agro-food Research and Technology, Autonomus University of Barcelona, University of Barcelona, Bellaterra (Cerdanyola del Vallés), 08193 Barcelona, Spain
References
- ↑ 1.0 1.1 Schmidt R, Mieulet D, Hubberten HM, Obata T, Hoefgen R, Fernie AR, Fisahn J, San Segundo B, Guiderdoni E, Schippers JH, Mueller-Roeber B. Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice. Plant Cell. 2013 Jun;25(6):2115-31. doi: 10.1105/tpc.113.113068. Epub 2013 Jun 25. PubMed PMID: 23800963; PubMed Central PMCID: PMC3723616.
