Difference between revisions of "Os01g0777300"
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==Annotated Information== | ==Annotated Information== | ||
| + | [[File:149-Os01g0777300.png|right|thumb|427px|'''Figure 2.''' ''Subcellular localization of the OsEXO1-GFP fusion protein in onion epidermal cells.<ref name="ref1" />.'']] | ||
===Function=== | ===Function=== | ||
| − | + | * '''''OsEXO1''''' is able to substitute for '''''ScEXO1''''' and '''''ScRAD27''''' functions. | |
| − | + | * Yeast two-hybrid analysis shows that OsEXO1 interacted with rice DNA polymerase k (OsPol k), the 70 kDa subunit b of rice replication protein A (OsRPA70b), and the 32 kDa subunit 1 of rice replication protein A (OsRPA32-1). | |
| + | * '''''OsEXO1''''' plays an important role in both cell proliferation and UV-damaged nuclear DNA repair pathway under dark conditions. | ||
===Expression=== | ===Expression=== | ||
| − | + | * The transcript of '''''OsEXO1''''' is highly expressed in meristematic tissues and panicles. | |
| − | + | * Inhibition of cell proliferation by removal of sucrose from the medium or by the addition of cell cycle inhibitors decreased '''''OsEXO1''''' expression. | |
| − | |||
| − | + | ===Subcellular localization=== | |
| − | + | * Subcellular localization analysis showed that '''''OsEXO1'''''-sGFP fusion protein transiently overexpressed in the onion epidermal cells localized to the nucleus. | |
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 04:02, 21 October 2016
The rice Os01g0777300 was reported as OsExo1 in 2008 [1] by researchers from Japan.
Contents
Annotated Information
Figure 2. Subcellular localization of the OsEXO1-GFP fusion protein in onion epidermal cells.[1].
Function
- OsEXO1 is able to substitute for ScEXO1 and ScRAD27 functions.
- Yeast two-hybrid analysis shows that OsEXO1 interacted with rice DNA polymerase k (OsPol k), the 70 kDa subunit b of rice replication protein A (OsRPA70b), and the 32 kDa subunit 1 of rice replication protein A (OsRPA32-1).
- OsEXO1 plays an important role in both cell proliferation and UV-damaged nuclear DNA repair pathway under dark conditions.
Expression
- The transcript of OsEXO1 is highly expressed in meristematic tissues and panicles.
- Inhibition of cell proliferation by removal of sucrose from the medium or by the addition of cell cycle inhibitors decreased OsEXO1 expression.
Subcellular localization
- Subcellular localization analysis showed that OsEXO1-sGFP fusion protein transiently overexpressed in the onion epidermal cells localized to the nucleus.
Labs working on this gene
- Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
- Section of Plant Biology, University of California, Davis, Davis, CA 95616, USA
- Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
- National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Ibaraki 305-8604, Japan
References
Please input cited references here. 1. Yong-Ik Kwon;Kiyomi Abe;Keishi Osakabe;Masaki Endo;Ayako Nishizawa-Yokoi;Hiroaki Saika;Hiroaki Shimada;Seiichi Toki Overexpression of OsRecQl4 and/or OsExo1 Enhances DSB-Induced Homologous Recombination in Rice Plant and Cell Physiology, 2012, 53(12): 2142-2152 2. Tomoyuki Furukawa;Tomohiro Imamura;Hiroko K. Kitamoto;Hiroaki Shimada Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase λ and RPA subunit proteins, is involved in cell proliferation Plant Molecular Biology, 2008, 66(5): 519-531 3.Furukawa, T., Imamura, T., Kitamoto, H.K. and Shimada, H. (2008) Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is involved in cell proliferation. Plant Mol. Biol.66: 519–531. 4.Li, H.Q., Terada, R., Li, M.R. and Iida, S. (2004) RecQ helicase enhances homologous recombination in plants.FEBS Lett.574: 151–155. 5.Tishkoff, D.X., Amin, N.S., Viars, C.S., Arden, K.C. and Kolodner, R.D.(1998) Identification of a human gene encoding a homologue of Saccharomyces cerevisiae EXO1, an exonuclease implicated in mismatch repair and recombination.Cancer Res.58: 5027–5031.
