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| − | Please input one-sentence summary here.
| + | The rice '''''Os01g0777300''''' was reported as '''''OsExo1''''' in 2008 <ref name="ref1" /> by researchers from Japan. |
| | | | |
| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:149-Os01g0777300.png|right|thumb|327px|'''Figure 2.''' ''Subcellular localization of the OsEXO1-GFP fusion protein in onion epidermal cells.<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os01g0777300''''' '''<=>''' '''''OsExo1,Exo1''''' |
| | + | |
| | ===Function=== | | ===Function=== |
| − | In line #6, which showed approximately two blue spots per 100 mg of calli in the control, overexpression of I-SceI increased the number of GUS spots 2.3-fold, overexpression of OsExo1 increased GUS spots 3.6-fold and overexpression of OsRecQl4 increased GUS spots 2.5-fold. Overexpression of OsExo1or OsRecQl4 with I-SceI increased the number of GUS spots by 18- or 15-fold, respectively. Furthermore, we observed a synergistic effect of OsExo1 and OsRecQl4; a 29.8-fold increment was observed. However, in line #2, which showed approximately 20 blue spots per 100 mg of calli even in the control, no effects of the expression of each of the three genes were apparent. Nevertheless, both lines demonstrated that enhanced restoration of the GUS gene occurred upon DSB induction by I-SceI with OsRecQl4 or OsExo1. The results presented here suggest strongly that OsRecQl4 and OsExo1 enhance processing of the 50 end resection step after DSB induction by transiently expressed I-SceI and subsequent repair by HR and/or SSA in rice. The stable transformation of OsExo1 might be toxic for rice cells even when using the b-estradiol induction system. The overexpression of either OsRecQl4 or OsExo1 can enhance the processing of DSBs for efficient HR and SSA. BLM promotes resection of DNA strands in the 50-ssDNA resection step, and also removes Rad51 from ssDNA after invasion of the template.
| + | * '''''OsEXO1''''' is able to substitute for '''''ScEXO1''''' and '''''ScRAD27''''' functions. |
| − | | + | * Yeast two-hybrid analysis shows that '''''OsEXO1''''' interacted with rice DNA polymerase k (OsPol k), the 70 kDa subunit b of rice replication protein A ('''''OsRPA70b'''''), and the 32 kDa subunit 1 of rice replication protein A ('''''OsRPA32-1'''''). |
| | + | * '''''OsEXO1''''' plays an important role in both cell proliferation and UV-damaged nuclear DNA repair pathway under dark conditions. |
| | ===Expression=== | | ===Expression=== |
| − | We investigated the frequency of GUS gene restoration using the following expression constructs: Pubi::OsExo1::TrbcS, Pubi::OsRecQl4::TrbcS and P2X35S::I-SceI::Thsp. The OsExo1 or OsRecQl4constructs were inserted into the pZK2 binary vector, OsExo1and I-SceI were inserted in the pZK2 binary vector, OsRecQl4and I-SceI were inserted in the pZD202 binary vector, and OsExo1, OsRecQl4and I-SceI were inserted into pZD202.
| + | * The transcript of '''''OsEXO1''''' is highly expressed in meristematic tissues and panicles. |
| − | | + | * Inhibition of cell proliferation by removal of sucrose from the medium or by the addition of cell cycle inhibitors decreased '''''OsEXO1''''' expression. |
| − | ===Evolution===
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| | | | |
| − | Overexpression of OsRecQl4 and/or OsExo1 with the combination of I-SceI digestion further enhances the frequency of GUS restoration, probably due to enhanced resection of the I-SceI sites. The overexpression of either OsRecQl4 or OsExo1 alone, without I-SceI digestion, leads to increased numbers of GUS spots. This
| + | ===Subcellular localization=== |
| − | result suggests that spontaneously occurring DSBs in the GU–US construct were also progressively processed by overexpressed OsRecQl4 or OsExo1.
| + | * Subcellular localization analysis showed that '''''OsEXO1'''''-sGFP fusion protein transiently overexpressed in the onion epidermal cells localized to the nucleus. |
| | | | |
| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan |
| | + | * Section of Plant Biology, University of California, Davis, Davis, CA 95616, USA |
| | + | * Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan |
| | + | * National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Ibaraki 305-8604, Japan |
| | + | ==References== |
| | + | <references> |
| | + | * <ref name="ref1"> |
| | + | Furukawa T, Imamura T, Kitamoto HK, Shimada H. Rice exonuclease-1 homologue, |
| | + | OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is |
| | + | involved in cell proliferation. Plant Mol Biol. 2008 Mar;66(5):519-31. doi: |
| | + | 10.1007/s11103-008-9288-6. Epub 2008 Jan 18. PubMed PMID: 18231866. |
| | | | |
| − | ==References==
| + | </ref> |
| − | Please input cited references here.
| + | </references> |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]] |
| − | GeneName = Os01g0777300|
| |
| − | Description = DNA repair protein (XPGC)/yeast Rad family protein|
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| − | Version = NM_001050956.2 GI:297597716 GeneID:4327899|
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| − | Length = 6382 bp|
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| − | Definition = Oryza sativa Japonica Group Os01g0777300, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 1|Chromosome 1]]|
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| − | AP = Chromosome 1:34649248..34655629|
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| − | CDS = 34649281..34649441,34650177..34650296,34650566..34650689,34650812..34650949,34651080..34651289<br>,34651673..34651847,34652105..34652178,34652625..34652751,34653043..34653389<br>,34653497..34653551,34653675..34653970,34654068..34654119,34654328..34654535<br>,34654652..34655075|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008394:34649248..34655629
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| − | source=RiceChromosome01
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008394:34649248..34655629
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| − | source=RiceChromosome01
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atggggatccagggattgctgccgcagctgaagtcgatcatggcgcccatcggggtggaggcgctcaagggccagacggtggccgtcgacacctactcctggctccacaagggcgccctctcctgcggcgaccgcctctgcaagggcctacccaccaccaggcatattgagtattgcatgcatagggttaacatgttgcggcaccatggcgtgaaaccaatccttgtatttgacggaggtcatctgccgatgaagggtgatcaagagactaaacgtgaaaggtcacgaaaggaaaatcttgagcgtgccaaggagcatgaatctgctgggaattcacgtgctgcttttgagtgttatcagaaagctgttgacattacacctaggattgcttttgaactgatccaggtattgaagcaagaaaaggttgattatattgttgcgccttatgaagcagatgcacaaatgacattcttgtctgtgaacaaacttgttgatgccgttatcacagaggattctgatctgataccatttggctgttctagaattattttcaagatggacaagtttggacaaggtgttgaatttcatattacacgattgcagcgatgcagggagcttgatcttaatggattcactatgcaaatgctactagagatgtgtattttaagtggctgtgattatcttccatcattgcctggaatgggtgtgaaacgagctcatgcacttatccaaaagctcaagggtcatgaaaaggtaatcaagcacctgaggtacagtgctgtttctgttccacctcaatatgaagaaaacttcagaaaggcgatctgggcatttcagttccaaagagtttatgaccctgtgacggaagatattgttcatttgtcaggcattccccatggcagtagtgaagacttggactttttgggcccatggctaccacagactgttgctaaaggcattgcccaagggaatatcgatccgattaccaaggaaccatttgagggcaaaacagagagtagcgcactcgcttttgataaagtgcatctgaacagagagtccagtgctccttcaaatggaaagaaaaagcttgatctacctgtccaaagaaacgtattgacaaattacttctgcttagcatcgcttgaggcaaaacggaagttcagagcacctaaggttacacctaagcaacaagtacttaatgggtctttgcccagtcccagaattgaagactctggtacccctgatttaattgaagatactagcttgccatcaaataacattcaagtttatcagtgtagttctgagcattttagctcaggaacccctctagatgattcaatcaataccgcctctcaatgtagttctgagcgtgtccgctgtgacatccctcgggatgattcagccagtgtttcaccccagtgttctcatgatattggtagtgatcctgctgaggacccagacattgaaggcaacaaggtgaaagtcaacttctgcaacagaagtacaataccgacaggctctttcttagaggggactttgcctgggatatcagatccatttttagattcacacaacacagagccatccagagctgcaccacgttatgctgaaaaaagcaatgtggtctctgccaatagaaatattactgtgaggagttcatatttcaaaacagtaaataaaagagtttgcacaaatcaaggagaagatgagtgccatgatgaagataactgcgagactggtaattacactcttcctggagatcaacaaagaagctctggaggtatattgaagagaagaaaatttagtgatccccaaaattttgaagatggaatgtttcaacccactagtcctcatgaaagtccaccggttgctgataaaggctgtgactctgattcccacgacggtatcaacacaaattctgaaggaaaatttggatgcaatgtcgcccatgtaaataaatacagtggcattgcagagaaatcaatggataagtttgctgcgctgatatcatctttcagatacgcaggctctcgtgccagtggtcttcgcgctcctttaaaggatgtgaagaacactcttcctgtcagatctgttctcagacctccagaacagagattcggttgtacggctaagaagactactagagtgcctctacaaagcagattctcaagtgatgctacaaacagcactgacgttcctgatctgagcacatttgcatacaggcctacaactgcttctgctcattctgatcaggggaagatcacaagcaaagctacagatgctgctgctggacctcctgacctgagaacatttgcttatgcaccaacaagatctaccaccagccgttttgatcagagtgaaaacacgcgcaaagctatgtgcactgcggacagccctcctgatattagcacattcgaatataaacccatgaaaagcgccgtcagacgttcagatgggagcaagttttcaggtgcagcactaaaagcagctagacgaacctctcggagctga</cdnaseq>|
| |
| − | AA = <aaseq>MGIQGLLPQLKSIMAPIGVEALKGQTVAVDTYSWLHKGALSCGD RLCKGLPTTRHIEYCMHRVNMLRHHGVKPILVFDGGHLPMKGDQETKRERSRKENLER AKEHESAGNSRAAFECYQKAVDITPRIAFELIQVLKQEKVDYIVAPYEADAQMTFLSV NKLVDAVITEDSDLIPFGCSRIIFKMDKFGQGVEFHITRLQRCRELDLNGFTMQMLLE MCILSGCDYLPSLPGMGVKRAHALIQKLKGHEKVIKHLRYSAVSVPPQYEENFRKAIW AFQFQRVYDPVTEDIVHLSGIPHGSSEDLDFLGPWLPQTVAKGIAQGNIDPITKEPFE GKTESSALAFDKVHLNRESSAPSNGKKKLDLPVQRNVLTNYFCLASLEAKRKFRAPKV TPKQQVLNGSLPSPRIEDSGTPDLIEDTSLPSNNIQVYQCSSEHFSSGTPLDDSINTA SQCSSERVRCDIPRDDSASVSPQCSHDIGSDPAEDPDIEGNKVKVNFCNRSTIPTGSF LEGTLPGISDPFLDSHNTEPSRAAPRYAEKSNVVSANRNITVRSSYFKTVNKRVCTNQ GEDECHDEDNCETGNYTLPGDQQRSSGGILKRRKFSDPQNFEDGMFQPTSPHESPPVA DKGCDSDSHDGINTNSEGKFGCNVAHVNKYSGIAEKSMDKFAALISSFRYAGSRASGL RAPLKDVKNTLPVRSVLRPPEQRFGCTAKKTTRVPLQSRFSSDATNSTDVPDLSTFAY RPTTASAHSDQGKITSKATDAAAGPPDLRTFAYAPTRSTTSRFDQSENTRKAMCTADS PPDISTFEYKPMKSAVRRSDGSKFSGAALKAARRTSRS</aaseq>|
| |
| − | DNA = <dnaseqindica>34..194#930..1049#1319..1442#1565..1702#1833..2042#2426..2600#2858..2931#3378..3504#3796..4142#4250..4304#4428..4723#4821..4872#5081..5288#5405..5828#gagagagagagagagagagagagagaggcggcaatggggatccagggattgctgccgcagctgaagtcgatcatggcgcccatcggggtggaggcgctcaagggccagacggtggccgtcgacacctactcctggctccacaagggcgccctctcctgcggcgaccgcctctgcaagggcctacccaccaccaggttcggctccggtgacctccccccttttcttggaacccagggtttcttaatccatttcgccgattggtgagacctaaatcgacggatccgggaaatctacttcttccccacgagcgtattgacagctgctggtcctaccaggggtttcttccgattttgttttcttggtcccgactggtgaaatccatttcggaggaggggtaaaaggcacgtcttgcttcggatgagagatttccgcattttttaggtgggtgtaggaaggggggggggatttcttgtttgttcagtctctctcaggattaaggtaataaatgtgcggcatttgttttcgcccccttccaggggcagtacatttactgaaatttgttatccgcctcagtgcccagattcttgtatgtctaggttgcattgaataaaatacacacttgggagagatccgctgcttggcatctggtgccgctttagttcagagaattcaccattttagtttgtggaatttttattggaaatagcttagacatgaatagttcaataaacgttaacaatagcttagcattgttagtagtatctttttactgtatgggatatcttctaacattgctgcatttttgatgtcgtgaactcataatgcggagggccatagatttgcaactccagtttttatcatcatttatcaccgttgtagttgagcaccataacttacaccttgtgtttcccctcctgaatctgaaccaggcatattgagtattgcatgcatagggttaacatgttgcggcaccatggcgtgaaaccaatccttgtatttgacggaggtcatctgccgatgaagggtgatcaagagactaaacgtgaaaggtatgttctttcttagggctccagaaagagcctaacatgtttcagttgccaaggaacatctacattacccttacagaacattaaaaaaaattgtgatttgacattttacatacggctatagttatggacaactgtggctttaggctgatatagagcagtcgcattattagtacagactagtggataagctaaaggcctccagccattctttgctatgtgatgtttcttgagtcattggatgctaagcaagtaagcatgacatcctgtaggtcacgaaaggaaaatcttgagcgtgccaaggagcatgaatctgctgggaattcacgtgctgcttttgagtgttatcagaaagctgttgacattacacctaggattgcttttgaactgatccaggtaaaggagcaaattccatgtatttttaccaaatatttaccacctatttgaagcagttctagtcaggagtaggaacatttggcttgtcttaactgattattgttccaaaacttgcaatgcaggtattgaagcaagaaaaggttgattatattgttgcgccttatgaagcagatgcacaaatgacattcttgtctgtgaacaaacttgttgatgccgttatcacagaggattctgatctgataccatttggctgttctagagtaagcacagtgttaaaacgctttatctttgtcctccacgtaccccatagtattttgctgctcagctagattagtgttcatagtttgttgtaacttctctaattaaaatgcatcttattccattgtgcagattattttcaagatggacaagtttggacaaggtgttgaatttcatattacacgattgcagcgatgcagggagcttgatcttaatggattcactatgcaaatgctactagagatgtgtattttaagtggctgtgattatcttccatcattgcctggaatgggtgtgaaacgagctcatgcacttatccaaaagctcaagggtcatgaaaaggtaaaccctgtatctgttgtatcaaatactaactttaggcctagttggaattggaaagaactgattcatgaactaacatgaacgatgcctgagatagtgttcaagacctacaaagcatggtaagaaaaggctggatagtagaaccagaatgcagttctacttagacataactacagaaggcaaggcctcaacacaaaattgattgcttcaacttttatgaggccaccgtcttgtacttaggtcattagccaatccatagcctgtcatacatgcaggacatgatttgagttactactgagcttgtagggcaacgatgtgcaatcactttcttgcttagcaaattagaaaaaaaaataatgagcaaatgatgcgtataatttcaggtaatcaagcacctgaggtacagtgctgtttctgttccacctcaatatgaagaaaacttcagaaaggcgatctgggcatttcagttccaaagagtttatgaccctgtgacggaagatattgttcatttgtcaggcattccccatggcagtagtgaagacttggactttttgggcccatatccttgccaaacttatatttgtacaatagatgctaaagctcattcatatgataacctgttgttttatccaccataagtccataaggatgaatttccacttttgtttggtcatgtctaatggccattatacaagttgcgttagaaagtaagctaacggcctttcaatgccaccatttctcagtaataccgaattgtgttacttgaagaaaatctcaacttgcatgcattggatcttttccttaattggtttatacatggctaccacagactgttgctaaaggcattgcccaagggaatatcgatccgattaccaaggaaccatttgaggtactaccgatcttccctcggttgttttggtttcttggtgctttgtttccttgaatgtaatttcatcctactctgattctgtgcactgcatatgtataaacatacccaagcatcaatatgcaaatacaaacgaggggctcttgaaaatggttgtaagctcactgaaatatccaatcagaggctacagagcattacagaagctcattagagaaaaacagataaaaatccataaaatattttgtaagaaaaaaatgtagggtcctcactgaaataagaaagtacattgtaagtgatcatggttttactgatgtactctgttttattaaagatcaccagtatgcaagcatttgctttgctgacatgcgaataccaacattcaattttatcttttgtggattgtcttactaagaagttatatatgttttatttgtatatgtgacttgcagggcaaaacagagagtagcgcactcgcttttgataaagtgcatctgaacagagagtccagtgctccttcaaatggaaagaaaaagcttgatctacctgtccaaagaaacgtattgacaaattacttctgtatcctctcaacacattttaatttaattgagagaacaatatatataccaatcaactttattatatgaactacttgttgcagcttatattatgggaatgccttggataagatagttcagttattgtgctttctcgttcccaaacatgtttaggatcaaatgaagaactaataatttccccataaacttatttacgaagtctaaggtgatgtgcgattcttagcatggtattttgcttgttttgtaaacaatacttcaactgtccattggatgaatccttgacttttagaaggcttagcatcgcttgaggcaaaacggaagttcagagcacctaaggttacacctaagcaacaagtacttaatgggtctttgcccagtcccagaattgaagactctggtacccctgatttaattgaagatactagcttgccatcaaataacattcaagtttatcagtgtagttctgagcattttagctcaggaacccctctagatgattcaatcaataccgcctctcaatgtagttctgagcgtgtccgctgtgacatccctcgggatgattcagccagtgtttcaccccagtgttctcatgatattggtagtgatcctgctgaggacccagacattgaaggcaacaaggtatttcacgtttctttattgccttattttcagtgttctcgtagtcctacctttctacaacaatatcgtatttccgtcattcttatagttttacttctgctatacaggtgaaagtcaacttctgcaacagaagtacaataccgacaggctctttcttagagggtaagagctctacctttttgtagttgatgcatgacttgatctgcaaatcatgtactggtatatctaattaaaatgaaaataatcttggtttctgttttgtatgcatttattttcgttttctagggactttgcctgggatatcagatccatttttagattcacacaacacagagccatccagagctgcaccacgttatgctgaaaaaagcaatgtggtctctgccaatagaaatattactgtgaggagttcatatttcaaaacagtaaataaaagagtttgcacaaatcaaggagaagatgagtgccatgatgaagataactgcgagactggtaattacactcttcctggagatcaacaaagaagctctggaggtatattgaagagaagaaaatttagtgatccccaaaattttgaagatgtaagcaatttttttttgagttgttattctatcatcatatcgcagtgaactgtctgtatcattctactatctggtcttgaatgtactatttgtgcagggaatgtttcaacccactagtcctcatgaaagtccaccggttgctgataaaggtaatagttccacctagttgtctgttcttattctctgtgagaatgtgcacctttccttgttccatttctctatagataaacttgtgacgattacaagaaattcagtttttccaaaaacaacagaaggttctgccaatgtttgagtgtgcgtagttccctttttttttgttgctgagggaaatcttctgaatgtctgatactaatgcaggctgtgactctgattcccacgacggtatcaacacaaattctgaaggaaaatttggatgcaatgtcgcccatgtaaataaatacagtggcattgcagagaaatcaatggataagtttgctgcgctgatatcatctttcagatacgcaggctctcgtgccagtggtcttcgcgctcctttaaaggatgtgaagaacactcttcctgtcaggtctgcagcttcagaaaatttcaattgcaccaagttcacacaatttacttatcagaacttaagagggggacacagttgtcccaatgagttgatatattttataacattaattgcagatctgttctcagacctccagaacagagattcggttgtacggctaagaagactactagagtgcctctacaaagcagattctcaagtgatgctacaaacagcactgacgttcctgatctgagcacatttgcatacaggcctacaactgcttctgctcattctgatcaggggaagatcacaagcaaagctacagatgctgctgctggacctcctgacctgagaacatttgcttatgcaccaacaagatctaccaccagccgttttgatcagagtgaaaacacgcgcaaagctatgtgcactgcggacagccctcctgatattagcacattcgaatataaacccatgaaaagcgccgtcagacgttcagatgggagcaagttttcaggtgcagcactaaaagcagctagacgaacctctcggagctgattctcacaaaattgtggatgcacctgccagcttccttatctgagcatgtttggatttgcgtatgccttctgtctgcgttcatgatctgagttcaacagaatcatccagcaacaaatgctctcaataaaagtgcattccattttgcccaaagtgcaagcacggctgaggtagctgccattcactactactagctgcagggcttatcagttcagacttcagagctcatccgtatctgcgcaatcagaagcacacatgttctgaagggaagtgctttatcttctcggtttccagagccaattatgtggcattttgcgagccaaatgtatgtgctcgtttttgccttgtccaatgaggttcttgtatgaacagcagtcgaatttgcgatgctatttaagccgcaaatgaggatactgttgtattgttcctttttgagaagatactgctgtatgttcattcttatacaagtgtgagcattccttcagaattcagatagcattactcttgtcaactattgatcaagatgttggaaagactttgaaaccggctgtttaagc</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001050956.2 RefSeq:Os01g0777300]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 1]]
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| − | [[Category:Chromosome 1]]
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