Difference between revisions of "Os01g0777300"

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Please input one-sentence summary here.
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The rice '''''Os01g0777300''''' was reported as '''''OsExo1''''' in 2008 <ref name="ref1" /> by researchers from Japan.  
  
 
==Annotated Information==
 
==Annotated Information==
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[[File:149-Os01g0777300.png|right|thumb|327px|'''Figure 2.''' ''Subcellular localization of the OsEXO1-GFP fusion protein in onion epidermal cells.<ref name="ref1" />.'']]
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===Gene Symbol===
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*'''''Os01g0777300''''' '''<=>''' '''''OsExo1,Exo1'''''
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===Function===
 
===Function===
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In line #6, which showed approximately two blue spots per 100 mg of calli in the control, overexpression of I-SceI increased the number of GUS spots 2.3-fold, overexpression of OsExo1 increased GUS spots 3.6-fold and overexpression of OsRecQl4 increased GUS spots 2.5-fold. Overexpression of OsExo1or OsRecQl4 with I-SceI increased the number of GUS spots by 18- or 15-fold, respectively. Furthermore, we observed a synergistic effect of OsExo1 and OsRecQl4; a 29.8-fold increment was observed. However, in line #2, which showed approximately 20 blue spots per 100 mg of calli even in the control, no effects of the expression of each of the three genes were apparent. Nevertheless, both lines demonstrated that enhanced restoration of the GUS gene occurred upon DSB induction by I-SceI with OsRecQl4 or OsExo1. The results presented here suggest strongly that OsRecQl4 and OsExo1 enhance processing of the 50 end resection step after DSB induction by transiently expressed I-SceI and subsequent repair by HR and/or SSA in rice. The stable transformation of OsExo1 might be toxic for rice cells even when using the b-estradiol induction system. The overexpression of either OsRecQl4 or OsExo1 can enhance the processing of DSBs for efficient HR and SSA. BLM promotes resection of DNA strands in the 50-ssDNA resection step, and also removes Rad51 from ssDNA after invasion of the template.
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* '''''OsEXO1''''' is able to substitute for '''''ScEXO1''''' and '''''ScRAD27''''' functions.
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* Yeast two-hybrid analysis shows that '''''OsEXO1''''' interacted with rice DNA polymerase k (OsPol k), the 70 kDa subunit b of rice replication protein A ('''''OsRPA70b'''''), and the 32 kDa subunit 1 of rice replication protein A ('''''OsRPA32-1''''').
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* '''''OsEXO1''''' plays an important role in both cell proliferation and UV-damaged nuclear DNA repair pathway under dark conditions.
 
===Expression===
 
===Expression===
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We investigated the frequency of GUS gene restoration using the following expression constructs: Pubi::OsExo1::TrbcS, Pubi::OsRecQl4::TrbcS and P2X35S::I-SceI::Thsp. The OsExo1 or OsRecQl4constructs were inserted into the pZK2 binary vector, OsExo1and I-SceI were inserted in the pZK2 binary vector, OsRecQl4and I-SceI were inserted in the pZD202 binary vector, and OsExo1, OsRecQl4and I-SceI were inserted into pZD202.
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* The transcript of '''''OsEXO1''''' is highly expressed in meristematic tissues and panicles.
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* Inhibition of cell proliferation by removal of sucrose from the medium or by the addition of cell cycle inhibitors decreased '''''OsEXO1''''' expression.
  
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===Evolution===
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===Subcellular localization===
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* Subcellular localization analysis showed that '''''OsEXO1'''''-sGFP fusion protein transiently overexpressed in the onion epidermal cells localized to the nucleus.
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Overexpression of OsRecQl4 and/or OsExo1 with the combination of I-SceI digestion further enhances the frequency of GUS restoration, probably due to enhanced resection of the I-SceI sites. The overexpression of either OsRecQl4 or OsExo1 alone, without I-SceI digestion, leads to increased numbers of GUS spots. This
 
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result suggests that spontaneously occurring DSBs in the GU–US construct were also progressively processed by overexpressed OsRecQl4 or OsExo1.
 
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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Please input related labs here.
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* Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
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* Section of Plant Biology, University of California, Davis, Davis, CA 95616, USA
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* Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
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* National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Ibaraki 305-8604, Japan
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==References==
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<references>
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* <ref name="ref1">
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Furukawa T, Imamura T, Kitamoto HK, Shimada H. Rice exonuclease-1 homologue,
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OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is
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involved in cell proliferation. Plant Mol Biol. 2008 Mar;66(5):519-31. doi:
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10.1007/s11103-008-9288-6. Epub 2008 Jan 18. PubMed PMID: 18231866.
  
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==References==
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</ref>
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Please input cited references here.
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</references>
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1. Yong-Ik Kwon;Kiyomi Abe;Keishi Osakabe;Masaki Endo;Ayako Nishizawa-Yokoi;Hiroaki Saika;Hiroaki Shimada;Seiichi Toki Overexpression of OsRecQl4 and/or OsExo1 Enhances DSB-Induced Homologous Recombination in Rice Plant and Cell Physiology, 2012, 53(12): 2142-2152
 
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2. Tomoyuki Furukawa;Tomohiro Imamura;Hiroko K. Kitamoto;Hiroaki Shimada Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase λ and RPA subunit proteins, is involved in cell proliferation Plant Molecular Biology, 2008, 66(5): 519-531
 
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3.Furukawa, T., Imamura, T., Kitamoto, H.K. and Shimada, H. (2008) Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is involved in cell proliferation. Plant Mol. Biol.66: 519–531.
 
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4.Li, H.Q., Terada, R., Li, M.R. and Iida, S. (2004) RecQ helicase enhances homologous recombination in plants.FEBS Lett.574: 151–155.
 
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5.Tishkoff, D.X., Amin, N.S., Viars, C.S., Arden, K.C. and Kolodner, R.D.(1998) Identification of a human gene encoding a homologue of Saccharomyces cerevisiae EXO1, an exonuclease implicated in mismatch repair and recombination.Cancer Res.58: 5027–5031.
 
  
 
==Structured Information==
 
==Structured Information==
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]

Latest revision as of 04:04, 21 October 2016

The rice Os01g0777300 was reported as OsExo1 in 2008 [1] by researchers from Japan.

Annotated Information

Figure 2. Subcellular localization of the OsEXO1-GFP fusion protein in onion epidermal cells.[1].

Gene Symbol

  • Os01g0777300 <=> OsExo1,Exo1

Function

  • OsEXO1 is able to substitute for ScEXO1 and ScRAD27 functions.
  • Yeast two-hybrid analysis shows that OsEXO1 interacted with rice DNA polymerase k (OsPol k), the 70 kDa subunit b of rice replication protein A (OsRPA70b), and the 32 kDa subunit 1 of rice replication protein A (OsRPA32-1).
  • OsEXO1 plays an important role in both cell proliferation and UV-damaged nuclear DNA repair pathway under dark conditions.

Expression

  • The transcript of OsEXO1 is highly expressed in meristematic tissues and panicles.
  • Inhibition of cell proliferation by removal of sucrose from the medium or by the addition of cell cycle inhibitors decreased OsEXO1 expression.

Subcellular localization

  • Subcellular localization analysis showed that OsEXO1-sGFP fusion protein transiently overexpressed in the onion epidermal cells localized to the nucleus.

Labs working on this gene

  • Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
  • Section of Plant Biology, University of California, Davis, Davis, CA 95616, USA
  • Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
  • National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Ibaraki 305-8604, Japan

References

  1. ↑ 1.0 1.1 Furukawa T, Imamura T, Kitamoto HK, Shimada H. Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is involved in cell proliferation. Plant Mol Biol. 2008 Mar;66(5):519-31. doi: 10.1007/s11103-008-9288-6. Epub 2008 Jan 18. PubMed PMID: 18231866.

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os01g0777300&oldid=275091"