Difference between revisions of "Os01g0777300"

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==Annotated Information==
 
==Annotated Information==
[[File:149-Os01g0777300.png|right|thumb|427px|'''Figure 2.''' ''Subcellular localization of the OsEXO1-GFP fusion protein in onion epidermal cells.<ref name="ref1" />.'']]
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[[File:149-Os01g0777300.png|right|thumb|327px|'''Figure 2.''' ''Subcellular localization of the OsEXO1-GFP fusion protein in onion epidermal cells.<ref name="ref1" />.'']]
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===Gene Symbol===
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*'''''Os01g0777300''''' '''<=>''' '''''OsExo1,Exo1'''''
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===Function===
 
===Function===
 
* '''''OsEXO1''''' is able to substitute for '''''ScEXO1''''' and '''''ScRAD27''''' functions.
 
* '''''OsEXO1''''' is able to substitute for '''''ScEXO1''''' and '''''ScRAD27''''' functions.
* Yeast two-hybrid analysis shows that OsEXO1 interacted with rice DNA polymerase k (OsPol k), the 70 kDa subunit b of rice replication protein A (OsRPA70b), and the 32 kDa subunit 1 of rice replication protein A (OsRPA32-1).
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* Yeast two-hybrid analysis shows that '''''OsEXO1''''' interacted with rice DNA polymerase k (OsPol k), the 70 kDa subunit b of rice replication protein A ('''''OsRPA70b'''''), and the 32 kDa subunit 1 of rice replication protein A ('''''OsRPA32-1''''').
 
* '''''OsEXO1''''' plays an important role in both cell proliferation and UV-damaged nuclear DNA repair pathway under dark conditions.
 
* '''''OsEXO1''''' plays an important role in both cell proliferation and UV-damaged nuclear DNA repair pathway under dark conditions.
 
===Expression===
 
===Expression===
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* National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Ibaraki 305-8604, Japan
 
* National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Ibaraki 305-8604, Japan
 
==References==
 
==References==
Please input cited references here.
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<references>
1. Yong-Ik Kwon;Kiyomi Abe;Keishi Osakabe;Masaki Endo;Ayako Nishizawa-Yokoi;Hiroaki Saika;Hiroaki Shimada;Seiichi Toki Overexpression of OsRecQl4 and/or OsExo1 Enhances DSB-Induced Homologous Recombination in Rice Plant and Cell Physiology, 2012, 53(12): 2142-2152
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* <ref name="ref1">
2. Tomoyuki Furukawa;Tomohiro Imamura;Hiroko K. Kitamoto;Hiroaki Shimada Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase λ and RPA subunit proteins, is involved in cell proliferation Plant Molecular Biology, 2008, 66(5): 519-531
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Furukawa T, Imamura T, Kitamoto HK, Shimada H. Rice exonuclease-1 homologue,
3.Furukawa, T., Imamura, T., Kitamoto, H.K. and Shimada, H. (2008) Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is involved in cell proliferation. Plant Mol. Biol.66: 519–531.
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OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is
4.Li, H.Q., Terada, R., Li, M.R. and Iida, S. (2004) RecQ helicase enhances homologous recombination in plants.FEBS Lett.574: 151–155.
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involved in cell proliferation. Plant Mol Biol. 2008 Mar;66(5):519-31. doi:
5.Tishkoff, D.X., Amin, N.S., Viars, C.S., Arden, K.C. and Kolodner, R.D.(1998) Identification of a human gene encoding a homologue of Saccharomyces cerevisiae EXO1, an exonuclease implicated in mismatch repair and recombination.Cancer Res.58: 5027–5031.
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10.1007/s11103-008-9288-6. Epub 2008 Jan 18. PubMed PMID: 18231866.
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</ref>
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</references>
  
 
==Structured Information==
 
==Structured Information==
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]]

Latest revision as of 04:04, 21 October 2016

The rice Os01g0777300 was reported as OsExo1 in 2008 [1] by researchers from Japan.

Annotated Information

Figure 2. Subcellular localization of the OsEXO1-GFP fusion protein in onion epidermal cells.[1].

Gene Symbol

  • Os01g0777300 <=> OsExo1,Exo1

Function

  • OsEXO1 is able to substitute for ScEXO1 and ScRAD27 functions.
  • Yeast two-hybrid analysis shows that OsEXO1 interacted with rice DNA polymerase k (OsPol k), the 70 kDa subunit b of rice replication protein A (OsRPA70b), and the 32 kDa subunit 1 of rice replication protein A (OsRPA32-1).
  • OsEXO1 plays an important role in both cell proliferation and UV-damaged nuclear DNA repair pathway under dark conditions.

Expression

  • The transcript of OsEXO1 is highly expressed in meristematic tissues and panicles.
  • Inhibition of cell proliferation by removal of sucrose from the medium or by the addition of cell cycle inhibitors decreased OsEXO1 expression.

Subcellular localization

  • Subcellular localization analysis showed that OsEXO1-sGFP fusion protein transiently overexpressed in the onion epidermal cells localized to the nucleus.

Labs working on this gene

  • Division of Plant Biotechnology, Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
  • Section of Plant Biology, University of California, Davis, Davis, CA 95616, USA
  • Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
  • National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Ibaraki 305-8604, Japan

References

  1. 1.0 1.1 Furukawa T, Imamura T, Kitamoto HK, Shimada H. Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is involved in cell proliferation. Plant Mol Biol. 2008 Mar;66(5):519-31. doi: 10.1007/s11103-008-9288-6. Epub 2008 Jan 18. PubMed PMID: 18231866.

Structured Information