Difference between revisions of "Os06g0211200"
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| − | + | The rice '''''Os06g0211200''''' was reported as '''''OsbZIP46''''' in 2012 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | * '''''OsbZIP46''''' is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa) | |
| + | * '''''OsbZIP46''''' is a positive regulator of ABA signaling and drought stress tolerance of rice depending on its activation. | ||
| + | * The stress-related genes activated by '''''OsbZIP46CA1''''' are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of '''''OsbZIP46CA1''''' in genetic engineering of drought tolerance. | ||
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * Overexpression of the native '''''OsbZIP46''''' gene increased ABA sensitivity but had no positive effect on drought resistance. | ||
| + | * The activation domain of '''''OsbZIP46''''' was defined by a series of deletions, and a region (domain D) was identified as having a negative effect on the activation. We produced a constitutive active form of '''''OsbZIP46''''' (OsbZIP46CA1) with a deletion of domain D. Overexpression of '''''OsbZIP46CA1''''' in rice significantly increased tolerance to drought and osmotic stresses. | ||
| + | * Gene chip analysis of the two overexpressors (native '''''OsbZIP46''''' and the constitutive active form '''''OsbZIP46CA'''''1) revealed that a large number of stress-related genes, many of them predicted to be downstream genes of ABF/ AREBs, were activated in the '''''OsbZIP46CA1 '''''overexpressor but not (even down-regulated) in the '''''OsbZIP46''''' overexpressor. | ||
| + | * '''''OsbZIP46''''' can interact with homologs of SnRK2 protein kinases that phosphorylate ABFs in Arabidopsis. | ||
===Expression=== | ===Expression=== | ||
| − | + | * Expression of '''''OsbZIP46''''' was strongly induced by drought, heat, hydrogen peroxide, and abscisic acid (ABA) treatment; however, it was not induced by salt and cold stresses. | |
===Evolution=== | ===Evolution=== | ||
| − | + | * '''''OsbZIP46''''' has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidopsis thaliana) and rice, respectively. | |
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China | |
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Tang N, Zhang H, Li X, Xiao J, Xiong L. Constitutive activation of | ||
| + | transcription factor OsbZIP46 improves drought tolerance in rice. Plant Physiol. | ||
| + | 2012 Apr;158(4):1755-68. doi: 10.1104/pp.111.190389. Epub 2012 Feb 1. PubMed | ||
| + | PMID: 22301130; PubMed Central PMCID: PMC3320183. | ||
| + | </ref> | ||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
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[[Category:Genes]] | [[Category:Genes]] | ||
[[Category:Japonica mRNA]] | [[Category:Japonica mRNA]] | ||
Latest revision as of 05:53, 22 October 2016
The rice Os06g0211200 was reported as OsbZIP46 in 2012 [1] by researchers from China.
Contents
Annotated Information
Function
- OsbZIP46 is one member of the third subfamily of bZIP transcription factors in rice (Oryza sativa)
- OsbZIP46 is a positive regulator of ABA signaling and drought stress tolerance of rice depending on its activation.
- The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of OsbZIP46CA1 in genetic engineering of drought tolerance.
Phenotypic analysis
- Overexpression of the native OsbZIP46 gene increased ABA sensitivity but had no positive effect on drought resistance.
- The activation domain of OsbZIP46 was defined by a series of deletions, and a region (domain D) was identified as having a negative effect on the activation. We produced a constitutive active form of OsbZIP46 (OsbZIP46CA1) with a deletion of domain D. Overexpression of OsbZIP46CA1 in rice significantly increased tolerance to drought and osmotic stresses.
- Gene chip analysis of the two overexpressors (native OsbZIP46 and the constitutive active form OsbZIP46CA1) revealed that a large number of stress-related genes, many of them predicted to be downstream genes of ABF/ AREBs, were activated in the OsbZIP46CA1 overexpressor but not (even down-regulated) in the OsbZIP46 overexpressor.
- OsbZIP46 can interact with homologs of SnRK2 protein kinases that phosphorylate ABFs in Arabidopsis.
Expression
- Expression of OsbZIP46 was strongly induced by drought, heat, hydrogen peroxide, and abscisic acid (ABA) treatment; however, it was not induced by salt and cold stresses.
Evolution
- OsbZIP46 has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidopsis thaliana) and rice, respectively.
You can also add sub-section(s) at will.
Labs working on this gene
- National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China
References
- ↑ Tang N, Zhang H, Li X, Xiao J, Xiong L. Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice. Plant Physiol. 2012 Apr;158(4):1755-68. doi: 10.1104/pp.111.190389. Epub 2012 Feb 1. PubMed PMID: 22301130; PubMed Central PMCID: PMC3320183.
