Difference between revisions of "Os06g0245600"

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(Evolution)
 
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*'''''Os06g0245600''''' '''''<=>''''' '''''OsRPL1,RPL1'''''
 
*'''''Os06g0245600''''' '''''<=>''''' '''''OsRPL1,RPL1'''''
 
===Function===
 
===Function===
* Analysis of the putative rice brassinosteroid receptor OsBRI1, a key hormone signaling gene, indicated that '''''RPL1''''' may be involved in the regulation of epigenomic modification of the gene.  
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* HSF binding protein (HSBP) is reported to modulate the function of '''''HSF''''' by binding to their trimer and hence to regulate HSR.  
* '''''RPL1''''' regulated phenotypic plasticity likely through its involvement in epigenetic processes affecting responses of the plant to phytohormones.
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* '''''OsHSBP1''''' and '''''OsHSBP2''''' play important role in the regulation of the HSR and seed development of rice.
 
===Phenotypic analysis===
 
===Phenotypic analysis===
* The rice (Oryza sativa L.) mutant, rice plasticity 1 (rpl1), that displayed increased environment-dependent phenotypic variations.
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* Both '''''OsHSBP1''''' and '''''OsHSBP2''''' were also important for seed development, as their knock-down lines were associated with significant seed abortion.  
* The rpl1 mutation led to an increase in DNA methylation on repetitive sequences and a decrease in overall histone acetylation. In addition, the mutation affected responses of the rice plant to phytohormones such as brassinosteroid, gibberellin, and cytokinin.
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* The thermotolerance assay revealed that '''''OsHSBP1''''' and '''''OsHSBP2''''' are negative regulators of HSR and involved in acquired thermotolerance but not in basal thermotolerance since their over-expression transgenic lines pre-heated at sublethal temperature, showed significantly decreased seedling survival after heat shock treatment.  
 
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* Furthermore, antioxidant activity and gene expression of catalase and peroxidase was significantly increased in knock-down transgenic seedlings of OsHSBP1 and OsHSBP2 after heat stress compared with the wild type.
 
===Expression===
 
===Expression===
* RPL1 was expressed in all tissues examined.  
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* Both '''''OsHSBP1''''' and '''''OsHSBP2''''' expressed ubiquitously in all tissues under normal growth conditions while their expression levels were significantly increased during recovery after heat shock treatment.  
  
 
===Subcellular localization===
 
===Subcellular localization===
* The protein was localized in the nucleus and its distribution in the nucleus overlapped with heterochromatin.
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* Subcellular localization analysis revealed the cytosol-nuclear localization of both '''''OsHSBP1''''' and '''''OsHSBP2''''' in onion epidermal cells.  
 
 
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==Labs working on this gene==
 
==Labs working on this gene==

Latest revision as of 11:29, 22 October 2016

The rice Os06g0245600 was reported as RPL1 in 2011 [1] by researchers from China.

Annotated Information

Gene Symbol

  • Os06g0245600 <=> OsRPL1,RPL1

Function

  • HSF binding protein (HSBP) is reported to modulate the function of HSF by binding to their trimer and hence to regulate HSR.
  • OsHSBP1 and OsHSBP2 play important role in the regulation of the HSR and seed development of rice.

Phenotypic analysis

  • Both OsHSBP1 and OsHSBP2 were also important for seed development, as their knock-down lines were associated with significant seed abortion.
  • The thermotolerance assay revealed that OsHSBP1 and OsHSBP2 are negative regulators of HSR and involved in acquired thermotolerance but not in basal thermotolerance since their over-expression transgenic lines pre-heated at sublethal temperature, showed significantly decreased seedling survival after heat shock treatment.
  • Furthermore, antioxidant activity and gene expression of catalase and peroxidase was significantly increased in knock-down transgenic seedlings of OsHSBP1 and OsHSBP2 after heat stress compared with the wild type.

Expression

  • Both OsHSBP1 and OsHSBP2 expressed ubiquitously in all tissues under normal growth conditions while their expression levels were significantly increased during recovery after heat shock treatment.

Subcellular localization

  • Subcellular localization analysis revealed the cytosol-nuclear localization of both OsHSBP1 and OsHSBP2 in onion epidermal cells.

Labs working on this gene

  • National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China

References

  1. Zhang CC, Yuan WY, Zhang QF. RPL1, a gene involved in epigenetic processes regulates phenotypic plasticity in rice. Mol Plant. 2012 Mar;5(2):482-93. doi: 10.1093/mp/ssr091. Epub 2011 Nov 29. PubMed PMID: 22131052.

Structured Information