Difference between revisions of "Os09g0547500"

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(Created page with "{{JaponicaGene| GeneName = Os09g0547500| Description = Similar to Lysine decarboxylase-like protein| Version = NM_001070385.1 GI:115480512 GeneID:4347769| Length = 2002 bp...")
 
 
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{{JaponicaGene|
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The rice '''''Os09g0547500''''' was reported as '''''OsLDC-like 1''''' in 2012 <ref name="ref1" /> by researchers from Korea.  
GeneName = Os09g0547500|
 
Description = Similar to Lysine decarboxylase-like protein|
 
Version = NM_001070385.1 GI:115480512 GeneID:4347769|
 
Length = 2002 bp|
 
Definition = Oryza sativa Japonica Group Os09g0547500, complete gene.|
 
Source = Oryza sativa Japonica Group
 
  
  ORGANISM  Oryza sativa Japonica Group
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==Annotated Information==
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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===Gene Symbol===
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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*'''''Os09g0547500''''' '''<=>''' '''''OsLDC-like 1,ABCC1,OsMRP1,MRP1'''''
            clade; Ehrhartoideae; Oryzeae; Oryza.
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===Function===
|
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* The rice '''''OsLDC-like 1''''' encodes a lysine decarboxylase-like protein.
Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|
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AP = Chromosome 9:22474370..22476371|
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===Phenotypic analysis===
CDS = 22474444..22474591,22474725..22474822,22474943..22474973,22475081..22475395,22475974..22476134<br>|
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* The mutant line exhibited enhanced accumulation of the polyamines (PAs) putrescine, spermidine, and spermine under conditions of oxidative stress.  
GCID = <gbrowseImage1>
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* The generation of reactive oxygen species (ROS) in the mutant line was assessed by qRT-PCR analysis of NADPH oxidase (RbohD and RbohF), and by DCFH-DA staining.  
name=NC_008402:22474370..22476371
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* Cellular levels of ROS in osldc-like 1 leaves were significantly lower than those in the wild-type (WT) rice after exposure to oxidative, high salt and acid stresses. Exogenously-applied PAs such as spermidine and spermine significantly inhibited the stress-induced accumulation of ROS and cell damage in WT leaves.  
source=RiceChromosome09
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* Additionally, the activities of ROS-detoxifying enzymes were increased in the homozygous mutant line in the presence or absence of H2O2.
preset=GeneLocation
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* Thus, mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype.
</gbrowseImage1>|
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GSID = <gbrowseImage2>
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You can also add sub-section(s) at will.
name=NC_008402:22474370..22476371
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source=RiceChromosome09
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==Labs working on this gene==
preset=GeneLocation
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* Department of Biology, Sunchon National University, Sunchon 540-742, Korea
</gbrowseImage2>|
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* Crop Biotech Institute Center, Department of Plant Systems Biotech, Kyung Hee University, Yongin 446-701, Korea
CDNA = <cdnaseq>tcgatcgatggaagaggcggcggcggcagcggacatgaacggcggcgtgcatcagtcccggttccggcgggtttgcgtgttctgcgggagcagctccggcaagcggcgcagctaccgcgacgccgccgtcgagctcggcaaggagctggtggcgaggaaggtggatttggtgtacggaggaggcagcctgggtctcatgggggaggtcgccgaggccgtgcgcaacggcggcggccacgtcattggcgtcatacctaccactctgatgggcaaggaggtaacgggagagacggtgggggaggtgcgggaggtggggagcatgcacgagaggaaggcggagatggcgaggaggtcggacgcgttcgtggcgctgccgggagggtacgggacgctggaggaggtggtggaggtgatcgcgtgggcgcagctgggcatccacgccaagcccgtcggcctcctcaacgtcgacggctactacgacttcctcctcgccttcgtcgacaaggccgtcgccgacggcttcatccccccttcccaccgccacctcttcgtctccgcccccgacgccccctccctcgtccacaagctcgaggagtacgtgccggtgtagcaggagggcgacccggagacgcccaagctgcgttgggagatcgagcagcaggcagccgtccaggtcgtcggctacagctcctcgctccacgcccagctagctattgcagattaatctctcgatctgaattacttagtaattaa</cdnaseq>|
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==References==
AA = <aaseq>SIDGRGGGGSGHERRRASVPVPAGLRVLREQLRQAAQLPRRRRR                    ARQGAGGEEGGFGVRRRQPGSHGGGRRGRAQRRRPRHWRHTYHSDGQGGNGRDGGGGA                    GGGEHAREEGGDGEEVGRVRGAAGRVRDAGGGGGGDRVGAAGHPRQARRPPQRRRLLR                    LPPRLRRQGRRRRLHPPFPPPPLRLRPRRPLPRPQARGVRAGVAGGRPGDAQAALGDR                    AAGSRPGRRLQLLAPRPASYCRLISRSELLSN</aaseq>|
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<references>
DNA = <dnaseqindica>75..222#356..453#574..604#712..1026#1605..1765#atgtacatctcctctccacacacttcacacttcacctcgatcgatcgatcgcccgcagtagttagcgagagtgatcgatcgatggaagaggcggcggcggcagcggacatgaacggcggcgtgcatcagtcccggttccggcgggtttgcgtgttctgcgggagcagctccggcaagcggcgcagctaccgcgacgccgccgtcgagctcggcaaggagctggtatatatgcgtgattaattaatttctctccatgttgatcatgattaattcatgcattcgatcgagctcgatcaatcagctctagttaactagctaattaattaactgattaagacgatgaattaactggtaggtggcgaggaaggtggatttggtgtacggaggaggcagcctgggtctcatgggggaggtcgccgaggccgtgcgcaacggcggcggccacgtcattgggtccgtacacataggcaccccctctcttcgttctcctcgtgttgcctagctagctcgtactctgtctgttcaatgatcaatccggcaatgtttaatcaatttctttcttgatgcatgcagcgtcatacctaccactctgatgggcaaggaggtaaagaattgatttcaacagctcattaatttcagtcatatatattgactagctaataattaattaaggtaatcaaatggtagaaattaatgatgaggtacgtgtaggtaacgggagagacggtgggggaggtgcgggaggtggggagcatgcacgagaggaaggcggagatggcgaggaggtcggacgcgttcgtggcgctgccgggagggtacgggacgctggaggaggtggtggaggtgatcgcgtgggcgcagctgggcatccacgccaagcccgtcggcctcctcaacgtcgacggctactacgacttcctcctcgccttcgtcgacaaggccgtcgccgacggcttcatccccccttcccaccgccacctcttcgtctccgcccccgacgccccctccctcgtccacaagctcgaggtatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatatacaggcttagcaatcttaaaattgtgatttttgtcgcggggataaaaaaacccgaaatatctggaatttcgatatttttttggctgacccttttttaagaattttgatgaattttgaatgaatttgaataaactttaatcaaattaaaaaaactgaaatttctgacgtgatatgtgcttgccggtaggggggcgaagttaccaaaaaattcaattaccatacacatctccatccatcaatcatcatcatcttcttcttctgcatcgatcgtatagtcgtaggccagtcgagagtattctcagtacagtcgtattattttgatgtaattggctatttgtggactctcgtatgctttctcgatagatatatctgtccttcgattccctggacaacacggcggcgatcggtccactgacctgatccgctagctatagctagttactgtttccaagctgagctgattcaaacatgaccagtgttttgacttgttttgtgcttgcaggagtacgtgccggtgtagcaggagggcgacccggagacgcccaagctgcgttgggagatcgagcagcaggcagccgtccaggtcgtcggctacagctcctcgctccacgcccagctagctattgcagattaatctctcgatctgaattacttagtaattaatctggttgactaagtagatcatcaacgagaggatgcagaggaggaacaggtacctagtacgtacttacgaattctaaaaaaaaaggtactctgtactcctcaattatctgcctttagctagtgtattatgtacgtgctctggttattgcttattgttagctggtctggctgcatgtactctatatatatatgatgcagttctgtacatatacatcgatcgatcgagcgtgtcgcttt</dnaseqindica>|
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* <ref name="ref1">
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001070385.1 RefSeq:Os09g0547500]|
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Jang, Su Jin, et al. "Increased polyamine biosynthesis enhances stress tolerance by preventing the accumulation of reactive oxygen species: T-DNA mutational analysis of Oryza sativa lysine decarboxylase-like protein 1." Molecules and cells 34.3 (2012): 251-262.
}}
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</ref>
[[Category:Genes]]
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[[Category:Japonica mRNA]]
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</references>
[[Category:Oryza Sativa Japonica Group]]
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[[Category:Japonica Genes]]
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[[Category:Japonica Chromosome 9]]
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==Structured Information==
[[Category:Chromosome 9]]
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[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 9]]

Latest revision as of 14:16, 22 October 2016

The rice Os09g0547500 was reported as OsLDC-like 1 in 2012 [1] by researchers from Korea.

Annotated Information

Gene Symbol

  • Os09g0547500 <=> OsLDC-like 1,ABCC1,OsMRP1,MRP1

Function

  • The rice OsLDC-like 1 encodes a lysine decarboxylase-like protein.

Phenotypic analysis

  • The mutant line exhibited enhanced accumulation of the polyamines (PAs) putrescine, spermidine, and spermine under conditions of oxidative stress.
  • The generation of reactive oxygen species (ROS) in the mutant line was assessed by qRT-PCR analysis of NADPH oxidase (RbohD and RbohF), and by DCFH-DA staining.
  • Cellular levels of ROS in osldc-like 1 leaves were significantly lower than those in the wild-type (WT) rice after exposure to oxidative, high salt and acid stresses. Exogenously-applied PAs such as spermidine and spermine significantly inhibited the stress-induced accumulation of ROS and cell damage in WT leaves.
  • Additionally, the activities of ROS-detoxifying enzymes were increased in the homozygous mutant line in the presence or absence of H2O2.
  • Thus, mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype.

You can also add sub-section(s) at will.

Labs working on this gene

  • Department of Biology, Sunchon National University, Sunchon 540-742, Korea
  • Crop Biotech Institute Center, Department of Plant Systems Biotech, Kyung Hee University, Yongin 446-701, Korea

References

  1. Jang, Su Jin, et al. "Increased polyamine biosynthesis enhances stress tolerance by preventing the accumulation of reactive oxygen species: T-DNA mutational analysis of Oryza sativa lysine decarboxylase-like protein 1." Molecules and cells 34.3 (2012): 251-262.


Structured Information