Difference between revisions of "Os02g0818000"

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==Annotated Information==
 
==Annotated Information==
 
===Function===
 
===Function===
The '''CBS domain''' of ''UIP1'' is necessary for '''binding to 3' ''RbcS'''''. ''UIP1'' interacts with 3' untranslated region of ''RbcS1'' mRNA and '''increases tolerance''' of transgenic overexpressors to '''drought stress'''<ref name="ref1"/>.
+
* The rice '''''UIP1''''' encodes a novel RNA-binding protein (RBP) possessing a CBS (cystathionine-b-synthase) domain.
 +
* '''''UIP1''''' interacts with 3'-untranslated region of RbcS1 mRNA and increases tolerance of transgenic overexpressors to drought stress.
  
===Mutation===
+
===Phenotypic analysis===
transgenic and nontransgenic (NT) control plants<ref name="ref1"/>:
+
* Transgenic overexpression of '''''UIP1''''' in rice resulted in an increase in tolerance to drought stress at the vegetative stage of growth. Under drought, high salt and low temperature conditions, the maximum photochemical efficiency of photosystem II (Fv/Fm ) of '''''UIP1''''' plants was higher than those of the nontransgenic plants.
*During drought stress conditions, NT plants started to show '''visual symptoms''' of drought-induced '''damage''' such as leaf rolling and wilting with a concomitant loss of chlorophylls at an earlier stage than the ''OsCc1:UIP1'' plants.
 
*Upon rewatering, transgenic plants '''recovered well''' compared to the NT plants. Consequently, NT plants remained severely affected by drought stress while transgenic lines partially recovered after 3 days of rewatering.
 
*The Fv/Fm values of the ''OsCc1:UIP1'' plants were '''higher''' than those of the NT plants under the time course of stress treatments.
 
 
 
===Expression===
 
*Transgenic overexpression of ''UIP1'' in rice resulted in an '''increase in tolerance to drought stress''' at the '''vegetative stage''' of growth. Under '''drought''', '''high salt''' and '''low temperature''' conditions, the maximum photochemical efficiency of photosystem II (Fv/Fm) of UIP1 plants was '''higher''' than those of the nontransgenic plants<ref name="ref1"/>.
 
 
 
*Interestingly, the effect of ''UIP1'' overexpression on tolerance to stress was much '''more pronounced''' under drought than under high salt and low temperature conditions<ref name="ref1"/>.
 
 
 
===Knowledge Extension===
 
The 3'RbcS is the major mRNA region involved in a phenomenon referred to as stress-induced mRNA decay (SMD) under drought and salt stress conditions. SMD was observed on the mRNAs of photosynthetic genes (e.g., RbcS1, Cab1, and PCY) that are rapidly degraded when
 
plants are exposed to stress<ref name="ref2"/>.
 
  
 
==Labs working on this gene==
 
==Labs working on this gene==
 
*School of Biotechnology and Environmental Engineering,  Myongji University, Yongin 449-728, Korea
 
*School of Biotechnology and Environmental Engineering,  Myongji University, Yongin 449-728, Korea
 +
==References==
 +
<references>
 +
* <ref name="ref1">
 +
Park, Su-Hyun, et al. "Transgenic overexpression of UIP1, an interactor of the 3′ untranslated region of the Rubisco small subunit mRNA, increases rice tolerance to drought." Plant biotechnology reports 7.1 (2013): 83-90.
 +
</ref>
  
==Structured Information==
+
</references>
    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]
 
  
 
==Structured Information==
 
==Structured Information==

Revision as of 13:11, 28 October 2016

UIP1 (3'-UTR-interacting protein 1) acts as a chaperone protein of RbcS1 transcripts by associating with the RbcS1 3'-UTR in an event of SMD leading them to either PBs or SGs[1].

Annotated Information

Function

  • The rice UIP1 encodes a novel RNA-binding protein (RBP) possessing a CBS (cystathionine-b-synthase) domain.
  • UIP1 interacts with 3'-untranslated region of RbcS1 mRNA and increases tolerance of transgenic overexpressors to drought stress.

Phenotypic analysis

  • Transgenic overexpression of UIP1 in rice resulted in an increase in tolerance to drought stress at the vegetative stage of growth. Under drought, high salt and low temperature conditions, the maximum photochemical efficiency of photosystem II (Fv/Fm ) of UIP1 plants was higher than those of the nontransgenic plants.

Labs working on this gene

  • School of Biotechnology and Environmental Engineering, Myongji University, Yongin 449-728, Korea

References

  1. Park, Su-Hyun, et al. "Transgenic overexpression of UIP1, an interactor of the 3′ untranslated region of the Rubisco small subunit mRNA, increases rice tolerance to drought." Plant biotechnology reports 7.1 (2013): 83-90.

Structured Information