Difference between revisions of "Os03g0733800"

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(Created page with "{{JaponicaGene| GeneName = Os03g0733800| Description = Endoplasmic reticulum oxidoreductin 1 family protein| Version = NM_001057710.2 GI:297601639 GeneID:4334011| Length =...")
 
(Mutation)
 
(6 intermediate revisions by 3 users not shown)
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{{JaponicaGene|
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The rice '''Os03g0733800''' was reported as '''Ero1''' in 2005 <ref name="ref1" /> by researchers from the USA.
GeneName = Os03g0733800|
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<br>
Description = Endoplasmic reticulum oxidoreductin 1 family protein|
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==Annotated Information==
Version = NM_001057710.2 GI:297601639 GeneID:4334011|
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===Gene Symbol===
Length = 1498 bp|
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*'''Os03g0733800<=>Ero1'''
Definition = Oryza sativa Japonica Group Os03g0733800, complete gene.|
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<br>
Source = Oryza sativa Japonica Group
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===Function===
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*The formation of native disulfide bonds in proglutelins depends on an electron transfer pathway involving OsEro1 and OsPDIL.
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<br>
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===Mutation===
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*The RNAi knockdown of OsERO1 inhibited the formation of native disulfide bonds in the glutelin precursors (proglutelins) and promoted aggregation of the proglutelins through nonnative intermolecular disulfide bonds in the rER. Inhibition of the formation of native disulfide bonds was also observed in the seeds of the esp2 mutant, which lacks protein disulfide isomerase-like(PDIL)1;1, but shows enhanced OsEro1 expression. The researchers detected the generation of H2O2 in the rER of the WT subaleurone cells, whereas the rER-derived H2O2 levels decreased markedly in EM49 homozygous mutant seeds, which have fewer sulfhydryl groups than the WT seeds.
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<br>
  
  ORGANISM  Oryza sativa Japonica Group
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==Labs working on this gene==
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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*Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan;
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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*Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan
            clade; Ehrhartoideae; Oryzeae; Oryza.
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<br>
|
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==References==
Chromosome = [[:category:Japonica Chromosome 3|Chromosome 3]]|
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<references>
AP = Chromosome 3:30825494..30826991|
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<ref name="ref1">
CDS = 30825905..30826057,30826393..30826516,30826603..30826805,30826935..30826991|
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ER membrane-localized oxidoreductase Ero1 is required for disulfide bond formation in the rice endosperm
GCID = <gbrowseImage1>
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</ref>
name=NC_008396:30825494..30826991
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source=RiceChromosome03
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</references>
preset=GeneLocation
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<br>
</gbrowseImage1>|
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==Structured Information==
GSID = <gbrowseImage2>
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]
name=NC_008396:30825494..30826991
 
source=RiceChromosome03
 
preset=GeneLocation
 
</gbrowseImage2>|
 
CDNA = <cdnaseq>gagcgtgtacaaaatttgtacttcacttacctgtttgttcttcgggctgtgacaaaggcagcagattaccttgagcaggctgagtacaacaccggcaatcctgaagaggacttgaaaacacagtctttagtgaagcaattgctttacaaccccaaattaagatcagcgtgcccgttaccatttgatgaagcaaaactctggcaaggtgaaaatggccctgagctaaagcaggagattcagaagcaatttagaaatatcagtgcaattatggattgtgttggatgtgagaagtgtcgattatggggaaagctccaagttcttggtctcggtactgcattgaagattcttttctctgttgatggagagaaccatttgaatcaatcactccagctgcagcgaaatgaggtcattgcactagtaaaccttctgaataggctgtcagaatctgtcaactttgtacatgaaaaaggaccatcaattgaggatgtcattaaacaacagagctcttctactgtgaaaccggtgtttcctatctga</cdnaseq>|
 
AA = <aaseq>ERVQNLYFTYLFVLRAVTKAADYLEQAEYNTGNPEEDLKTQSLV                    KQLLYNPKLRSACPLPFDEAKLWQGENGPELKQEIQKQFRNISAIMDCVGCEKCRLWG                    KLQVLGLGTALKILFSVDGENHLNQSLQLQRNEVIALVNLLNRLSESVNFVHEKGPSI                    EDVIKQQSSSTVKPVFPI</aaseq>|
 
DNA = <dnaseqindica>935..1087#476..599#187..389#1..57#gagcgtgtacaaaatttgtacttcacttacctgtttgttcttcgggctgtgacaaaggttcctccttctcccttgctttaaaatcgaactctttgttttgcatggtactcatgtactacaaggttaagctgccaattttctgttctaaagcttgctgcatataatagttttatttggcttttgcaggcagcagattaccttgagcaggctgagtacaacaccggcaatcctgaagaggacttgaaaacacagtctttagtgaagcaattgctttacaaccccaaattaagatcagcgtgcccgttaccatttgatgaagcaaaactctggcaaggtgaaaatggccctgagctaaagcaggagattcagaagcaatttagaaatatcaggtgtgtgttcatgttattcatgttttcagttcataatatgcacttgtctgattcaaaatcatatccttggaatgttattttcacagtgcaattatggattgtgttggatgtgagaagtgtcgattatggggaaagctccaagttcttggtctcggtactgcattgaagattcttttctctgttgatggagagaaccatttgaatcaatcagtacgtatctcatttaatttttactcttggctttatcattatgcaacataaagtaatatgtttaagactcctttgtgttcaattggttggaccatgctccagtttgagtgctttatttggcagattgcgctcatattacctaattccttaacacttgctgttgatgatcacaaaaaatcttaagttgtggattgtcttaacaatttactagtagcagtagttactttttggcatgagagcaacaggaaatactgttcaaatttaccgttgaatacatttaagatgtttgcaatggtgatgagtctgacaatttgtcttctgggtaaattattcagctccagctgcagcgaaatgaggtcattgcactagtaaaccttctgaataggctgtcagaatctgtcaactttgtacatgaaaaaggaccatcaattgaggatgtcattaaacaacagagctcttctactgtgaaaccggtgtttcctatctgaatcgtgaaatagtaagtgttaatttttttcctcaggcagatttttttaaaaaaaaagacatactcataccctttcgttcttcaacatatggtttctgcaggcgttaaactgagatgtatttacaactttgatccatactagaacaatgatgtaggtgctggggtgaagcttactagagaattttgcagatcctagacagaagatatacagaactttgagatccaaacaattgcaattttcgatgtaaatcgtggtatttggcaatgggacctcttttgtaagggatcactgcccattttgcaatgttgcatgatcaaagagatgatgtcctaacatgcccatagacttcttcatgtgtaaactgtgaatctgtgatcatattgctaacaagcaaaagctttttgccaatct</dnaseqindica>|
 
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001057710.2 RefSeq:Os03g0733800]|
 
}}
 
[[Category:Genes]]
 
[[Category:Japonica mRNA]]
 
[[Category:Oryza Sativa Japonica Group]]
 
[[Category:Japonica Genes]]
 
[[Category:Japonica Chromosome 3]]
 
[[Category:Chromosome 3]]
 

Latest revision as of 11:03, 4 November 2016

The rice Os03g0733800 was reported as Ero1 in 2005 [1] by researchers from the USA.

Annotated Information

Gene Symbol

  • Os03g0733800<=>Ero1


Function

  • The formation of native disulfide bonds in proglutelins depends on an electron transfer pathway involving OsEro1 and OsPDIL.


Mutation

  • The RNAi knockdown of OsERO1 inhibited the formation of native disulfide bonds in the glutelin precursors (proglutelins) and promoted aggregation of the proglutelins through nonnative intermolecular disulfide bonds in the rER. Inhibition of the formation of native disulfide bonds was also observed in the seeds of the esp2 mutant, which lacks protein disulfide isomerase-like(PDIL)1;1, but shows enhanced OsEro1 expression. The researchers detected the generation of H2O2 in the rER of the WT subaleurone cells, whereas the rER-derived H2O2 levels decreased markedly in EM49 homozygous mutant seeds, which have fewer sulfhydryl groups than the WT seeds.


Labs working on this gene

  • Division of Plant Sciences, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan;
  • Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan


References

  1. ER membrane-localized oxidoreductase Ero1 is required for disulfide bond formation in the rice endosperm


Structured Information