Difference between revisions of "Os07g0187700"
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| − | + | The rice gene Os07g0187700 was reported as '''''OsPHF1''''' in 2011<ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os07g0187700 <=> OsPHF1, OsWD40-137, OsSEC12 | ||
| + | |||
===Function=== | ===Function=== | ||
| − | + | * '''''OsPHF1''''', a rice (Oryza sativa) gene homologous to '''''AtPHF1'''''. | |
| + | * '''''OsPHF1''''' is unique in the localization of both low- and high-affinity Pi transporters on the plasma membrane in rice and determines Pi uptake and translocation in rice. | ||
| + | ===Mutation=== | ||
| + | * Three '''''OsPHF1''''' allelic mutants carrying one-point mutations at the fifth WD-repeat motif and two at the transmembrane helix, respectively, showed arsenate resistance and severely reduced Pi accumulation. The data indicate that mutation of '''''OsPHF1''''' results in the endoplasmic reticulum retention of the low-affinity Pi transporter OsPT2 and highaffinity Pi transporter OsPT8. Mutation of '''''OsPHF1''''' also reduced Pi accumulation in plants exhibiting excessive shoot Pi accumulation due to the overexpression of OsPHR2. | ||
| − | ===Expression=== | + | ===Expression === |
| − | + | [[File:Os07g0187700-1.png|right|thumb|400px|'''Figure 4. Tissue-specific expression pattern of OsPHF1.''' '' <ref name="ref1" />.'']] | |
| + | * Reverse transcription (RT)-PCR analysis of OsPHF1 transcription indicates a constitutive expression pattern in plants (Fig. 4A). To detail the tissue-specific expression of OsPHF1, transgenic plants harboring a GUS reporter gene driven by the OsPHF1 promoter (23,700 bp upstream of the start codon) were developed. The GUS staining results were consistent with RT-PCR analysis (Fig. 4, B–F). Examinations of tissue cross sections indicated that OsPHF1 is expressed in the root cap (Fig. 4G), along with expression in the epidermis, exodermis, and sclerenchyma in the elongation zone of the primary root (Fig. 4H). Within the root region where lateral roots emerge, OsPHF1 is expressed in the cortex (Fig. 4I). GUS staining was also detected in the cortex of lateral roots (Fig. 4J) and all the mesophyll cells of the leaf (Fig. 4K). | ||
===Evolution=== | ===Evolution=== | ||
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You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
| + | ==Labs working on this gene== | ||
| + | * State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, People’s Republic of China | ||
| + | ==References== | ||
| + | <references> | ||
| + | * <ref name="ref1"> | ||
| + | Chen J, Liu Y, Ni J, Wang Y, Bai Y, Shi J, Gan J, Wu Z, Wu P. OsPHF1 regulates | ||
| + | the plasma membrane localization of low- and high-affinity inorganic phosphate | ||
| + | transporters and determines inorganic phosphate uptake and translocation in rice. | ||
| + | Plant Physiol. 2011 Sep;157(1):269-78. doi: 10.1104/pp.111.181669. PubMed PMID: | ||
| + | 21753117; PubMed Central PMCID: PMC3165875. | ||
| − | + | </ref> | |
| − | + | </references> | |
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==Structured Information== | ==Structured Information== | ||
| − | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 7]] | |
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| − | [[Category:Genes]] | ||
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| − | [[Category:Oryza Sativa Japonica Group]] | ||
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Latest revision as of 05:07, 11 December 2016
The rice gene Os07g0187700 was reported as OsPHF1 in 2011[1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os07g0187700 <=> OsPHF1, OsWD40-137, OsSEC12
Function
- OsPHF1, a rice (Oryza sativa) gene homologous to AtPHF1.
- OsPHF1 is unique in the localization of both low- and high-affinity Pi transporters on the plasma membrane in rice and determines Pi uptake and translocation in rice.
Mutation
- Three OsPHF1 allelic mutants carrying one-point mutations at the fifth WD-repeat motif and two at the transmembrane helix, respectively, showed arsenate resistance and severely reduced Pi accumulation. The data indicate that mutation of OsPHF1 results in the endoplasmic reticulum retention of the low-affinity Pi transporter OsPT2 and highaffinity Pi transporter OsPT8. Mutation of OsPHF1 also reduced Pi accumulation in plants exhibiting excessive shoot Pi accumulation due to the overexpression of OsPHR2.
Expression
Figure 4. Tissue-specific expression pattern of OsPHF1. [1].
- Reverse transcription (RT)-PCR analysis of OsPHF1 transcription indicates a constitutive expression pattern in plants (Fig. 4A). To detail the tissue-specific expression of OsPHF1, transgenic plants harboring a GUS reporter gene driven by the OsPHF1 promoter (23,700 bp upstream of the start codon) were developed. The GUS staining results were consistent with RT-PCR analysis (Fig. 4, B–F). Examinations of tissue cross sections indicated that OsPHF1 is expressed in the root cap (Fig. 4G), along with expression in the epidermis, exodermis, and sclerenchyma in the elongation zone of the primary root (Fig. 4H). Within the root region where lateral roots emerge, OsPHF1 is expressed in the cortex (Fig. 4I). GUS staining was also detected in the cortex of lateral roots (Fig. 4J) and all the mesophyll cells of the leaf (Fig. 4K).
Evolution
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, People’s Republic of China
References
- ↑ 1.0 1.1 Chen J, Liu Y, Ni J, Wang Y, Bai Y, Shi J, Gan J, Wu Z, Wu P. OsPHF1 regulates the plasma membrane localization of low- and high-affinity inorganic phosphate transporters and determines inorganic phosphate uptake and translocation in rice. Plant Physiol. 2011 Sep;157(1):269-78. doi: 10.1104/pp.111.181669. PubMed PMID: 21753117; PubMed Central PMCID: PMC3165875.
