Difference between revisions of "Os11g0687800"
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===Function=== | ===Function=== | ||
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* Both '''''Pik-1''''' and '''''Pik-2''''' encode coiled-coil nucleotide binding site leucine-rich repeat (NBS-LRR) proteins, and each shares a very high level of protein identity with corresponding proteins encoded by the '''''Pik-m''''' and '''''Pik-p''''' alleles. | * Both '''''Pik-1''''' and '''''Pik-2''''' encode coiled-coil nucleotide binding site leucine-rich repeat (NBS-LRR) proteins, and each shares a very high level of protein identity with corresponding proteins encoded by the '''''Pik-m''''' and '''''Pik-p''''' alleles. | ||
* A combination of '''''Pik-1''''' and '''''Pik-2''''' is required for the expression of Pik resistance. | * A combination of '''''Pik-1''''' and '''''Pik-2''''' is required for the expression of Pik resistance. | ||
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* Real-time RT-PCR analysis was used to characterize the expression of '''''Pik-1''''' and '''''Pik-2''''' in plants challenged by the Pik-avirulent blast isolate CHL381. Both were constitutively expressed, although at a low level. Following inoculation, the level of transcript for both genes declined further up to 12 h post-inoculation, thereafter increasing marginally to the steady-state level by 72 h post-inoculation. As a similar expression profile was also observed using a mock inoculation, it is possible that both genes were responding to abiotic stress. | * Real-time RT-PCR analysis was used to characterize the expression of '''''Pik-1''''' and '''''Pik-2''''' in plants challenged by the Pik-avirulent blast isolate CHL381. Both were constitutively expressed, although at a low level. Following inoculation, the level of transcript for both genes declined further up to 12 h post-inoculation, thereafter increasing marginally to the steady-state level by 72 h post-inoculation. As a similar expression profile was also observed using a mock inoculation, it is possible that both genes were responding to abiotic stress. | ||
===Evolution=== | ===Evolution=== | ||
| − | [[File:Os11g0687800-1.png| | + | [[File:Os11g0687800-1.png|center|thumb|400px|'''Fig. 7 Phylogeny of the Pik family, including rice and sorghum sequences sharing > 30% identity at the peptide level.''' '' <ref name="ref1" />.'']] |
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
Latest revision as of 01:43, 13 December 2016
The rice gene Os11g0687800 was reported as Pik1 in 2011[1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os11g0687800 <=> Pik1, OsPik1
Function
- Both Pik-1 and Pik-2 encode coiled-coil nucleotide binding site leucine-rich repeat (NBS-LRR) proteins, and each shares a very high level of protein identity with corresponding proteins encoded by the Pik-m and Pik-p alleles.
- A combination of Pik-1 and Pik-2 is required for the expression of Pik resistance.
Expression
- Real-time RT-PCR analysis was used to characterize the expression of Pik-1 and Pik-2 in plants challenged by the Pik-avirulent blast isolate CHL381. Both were constitutively expressed, although at a low level. Following inoculation, the level of transcript for both genes declined further up to 12 h post-inoculation, thereafter increasing marginally to the steady-state level by 72 h post-inoculation. As a similar expression profile was also observed using a mock inoculation, it is possible that both genes were responding to abiotic stress.
Evolution
Fig. 7 Phylogeny of the Pik family, including rice and sorghum sequences sharing > 30% identity at the peptide level. [1].
You can also add sub-section(s) at will.
Labs working on this gene
- Laboratory of Plant Resistance and Genetics, College of Resources and Environmental Sciences, South China Agricultural University, Guangzhou, 510642, China;
- Rice Research Institute, Guangdong Provincial Academy of Agricultural Sciences, Guangzhou, 510640, China
References
