Difference between revisions of "Os07g0153600"

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PROG1(PROSTRATE GROWTH 1),a  semi-dominant gene  controls aspects of wild-rice plant architecture, including tiller angle and number of tillers.
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The rice gene Os07g0153600 was reported as '''''PROG1''''' in 2008<ref name="ref1" /> by researchers from China.
  
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== Function ==
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==Annotated Information==
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===Gene Symbol===
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*'''''Os07g0153600 <=> PROG1, OsPROG1
  
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PROG1 variants identified in O. sativa disrupt the prog1 function and inactivate prog1 expression, leading to erect growth, greater grain number and higher grain yield in cultivated rice. Sequence comparison shows that 182 varieties of cultivated rice, including 87 indica and 95 japonica cultivars from 17 countries, carry identical mutations in the prog1 coding region that may have become fixed during rice domestication.
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===Function===
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[[File:Os07g0153600-2.png|right|thumb|400px|'''Figure 1 The transition from prostrate growth to erect growth. (a) Wild rice (O. rufipogon). (b) Cultivated rice (O. sativa).''' '' <ref name="ref1" />.'']]
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* The '''''PROG1''''' gene encodes a putative single Cys2-His2 zincfinger protein containing a highly conserved QALGGH motif specific to EPF zinc-finger proteins in plants and a leucine-rich motif that is similar to an EAR-like active repression domain at the C-terminal region
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* '''''PROG1''''' controls prostrate growth of wild rice from Yuanjiang County in China.
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* '''''PROG1''''' gene can decrease the number of primary and secondary branches, grain number and grain yield, and also suggest that the PROG1 gene was a key pleiotropic gene controlling plant architecture and yield-related traits in rice.
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===Mutation===
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* '''''prog1''''' variants identified in O. sativa disrupt the prog1 function and inactivate prog1 expression, leading to erect growth, greater grain number and higher grain yield in cultivated rice.
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===Expression ===
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[[File:Os07g0153600-1.png|right|thumb|400px|'''Figure 4 Expression of PROG1 and prog1.''' '' <ref name="ref1" />.'']]
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* The researchers detected GUS expression in the tiller base (Fig. 4b,c), leaf-sheath pulvinus (Fig. 4d,e) and lamina joint (Fig. 4f), but not in the root, leaf blade and culm.We also observed GFP activity and found that PROG1 was expressed strongly in the vascular bundles of the leaf-sheath pulvinus (Fig. 4g,h). Consistent with the expression patterns shown by GUS staining, they detected higher expression of PROG1 in the leafsheath pulvinus, tiller base and lamina joint by real-time quantitative PCR (Fig. 4i); these organs might directly respond to the development and maintenance of plant structure
  
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===Evolution===
  
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In the process of the evolution of the rice, wild rice by PROG1 genetic evolution of cultivated rice PROG1, gene function is lost, not only by the creeping growth to erect, be modified to improve plant type, and spike grain number increase, the yield increased significantly, and performance for sex. By sequence analysis found that 182 rice varieties from 17 countries of prog1 gene expression of the same variation, indicate that the genes may be the origin of the single.PROG1 gene detection and separation to reveal the molecular mechanism of rice evolution and study the molecular basis of rice plant type regulation is of great significance to (Tan et al., 2008).
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You can also add sub-section(s) at will.
  
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==Labs working on this gene==
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* 1State Key Laboratory of Plant Physiology and Biochemistry, National Center for Evaluation of Agricultural Wild Plants (Rice), Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100094, China.
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* MOE Key Laboratory of Bioinformatics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China.
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==References==
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<references>
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* <ref name="ref1">
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Tan L, Li X, Liu F, Sun X, Li C, Zhu Z, Fu Y, Cai H, Wang X, Xie D, Sun C.
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Control of a key transition from prostrate to erect growth in rice domestication.
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Nat Genet. 2008 Nov;40(11):1360-4. doi: 10.1038/ng.197. PubMed PMID: 18820699.
  
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Here some experiments to find the function of PROG1. They further developed a near-isogenic line, NIL(PROG1), that contained a very short PROG1chromosomal segment from wild rice in the Teqing genetic background and characterized the plant architecture (Fig. 1). During the early seedling stage, both tiller bud formation and tiller outgrowth in NIL(PROG1) were earlier than that in Teqing (Fig. 1a,b). After the tillering stage, NIL(PROG1)produced many tillers, but Teqing produced very few (Fig. 1c,d,f). Furthermore, NIL(PROG1) showed increased tiller spread with a wider tiller angle, whereas Teqing had a compact plant architecture with a narrower tiller angle (Fig. 1c,e). These results show that NIL(PROG1) possesses plant architecture similar to that of wild rice, including a prostrate growth (a wider tiller angle) and many tillers, suggesting that PROG1 may be a key domestication gene responsible for plant architecture.(Jin et al., 2008).
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</ref>
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[[File:fig1.jpg]]
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</references>
  
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== Localization ==
 
  
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PROG1 (PROSTRATE GROWTH 1), on chromosome 7 that encodes a single Cys2-His2 zinc-finger protein.
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==Structured Information==
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[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 07]]
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Genetic linkage analysis within 246 F2individuals derived from the cross between YIL18 and Teqing showed that prostrate growth was
 
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completely associated with a marked decrease of GNP and controlled by a single semi-dominant gene,PROG1(PROSTATE GROWTH 1), located between SSR markers RM298 and RM481 on short arm of chromosome 7 (Fig. 3a). Using a total of 3,600 recessive homozygote
 
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plants with erect growth from the F2population, we further delimited prog1 within an 8.8-kb region between the pr5 and pr7 markers
 
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(Fig. 3b). Within this region, there is only one hypothetical gene (LOC_Os07g05900) in the Nipponbare genome (the TIGR Rice
 
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Genome Annotation Database)9 (Fig. 3c). One positive BAC clone (YJ0710308) covering this region was isolated from genomic BAC
 
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library of YJCWR (Fig. 3d). We screened the YJ0710308 subclone library and identified two subclones, pPROG1-1 and pPROG1-2,
 
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containing the entire O. rufipogonLOC_Os07g05900 with 596-bp and 2,914-bp 5¢-flanking regions, respectively (Fig. 3e)(Jin et al., 2008).
 
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[[File:fig2.jpg]]
 
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PROG1 (PROSTRATE GROWTH 1), located in the rice chromosome 7 short arm SSR markers between RM298 and RM481 (Tan et al., 2008);Also between mark S1706 and RM7185 (Jin et al., 2008).Two independent team used two kinds of rice hybridization and introgression, after repeated increase of genetic map, they successfully separation and cloned gene PROG1.
 
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PROG1 cDNA 833 bp and contains a 486 - bp open reading frame (ORF), 147 - bp's 5 'end the translation section (untranslated region, UTR) and 200 - bp 3' UTR, encoding a 161 amino acid composition of Cys2 - His2 zinc finger protein (Tan et al., 2008).
 
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PROG1 encoding a 167 amino acid composition of zinc finger transcription factors, mainly in the axillary bud separatist group.In the gene encoding between wild rice and cultivated rice in hainan area has a base mutation amino acid replacement, speculated that the amino acid substitution in the process of artificial domestication is selected (Jin et al., 2008).
 
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== Expression ==
 
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PROG1 is predominantly expressed in the axillary meristems, the site of tiller bud formation. Rice transformation experiments demonstrate that artificial selection of an amino acid substitution in the PROG1 protein during domestication led to the transition from the plant architecture of wild rice(O.rufipogon) to that of domesticated rice(O.sativa).
 
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[[File:Wild_rice_(O._rufipogon).jpg‎]]
 
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[[File:Cultivated_rice_(O._sativa).jpg]]
 
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== References ==
 
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1. Lubin Tan;Xianran Li;Fengxia Liu;Xianyou Sun;Chenggang Li;Zuofeng Zhu;Yongcai Fu;Hongwei Cai;Xiangkun Wang;Daoxin Xie;Chuanqing Sun
 
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  Control of a key transition from prostrate to erect growth in rice domestication
 
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  Nature Genetics, 2008, 40(11): 1360-1364
 
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2. Jian Jin;Wei Huang;Ji-Ping Gao;Jun Yang;Min Shi;Mei-Zhen Zhu;Da Luo;Hong-Xuan Lin
 
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  Genetic control of rice plant architecture under domestication
 
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  Nature Genetics, 2008, 40(11): 1365-1369
 
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3. Yonghong Wang & Jiayang Li
 
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  Rice, rising
 
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  Nature Genetics, 2008, 40(11): 1273-1275
 
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== Structured Information ==
 

Latest revision as of 02:21, 13 December 2016

The rice gene Os07g0153600 was reported as PROG1 in 2008[1] by researchers from China.

Annotated Information

Gene Symbol

  • Os07g0153600 <=> PROG1, OsPROG1

Function

Figure 1 The transition from prostrate growth to erect growth. (a) Wild rice (O. rufipogon). (b) Cultivated rice (O. sativa). [1].
  • The PROG1 gene encodes a putative single Cys2-His2 zincfinger protein containing a highly conserved QALGGH motif specific to EPF zinc-finger proteins in plants and a leucine-rich motif that is similar to an EAR-like active repression domain at the C-terminal region
  • PROG1 controls prostrate growth of wild rice from Yuanjiang County in China.
  • PROG1 gene can decrease the number of primary and secondary branches, grain number and grain yield, and also suggest that the PROG1 gene was a key pleiotropic gene controlling plant architecture and yield-related traits in rice.

Mutation

  • prog1 variants identified in O. sativa disrupt the prog1 function and inactivate prog1 expression, leading to erect growth, greater grain number and higher grain yield in cultivated rice.

Expression

Figure 4 Expression of PROG1 and prog1. [1].
  • The researchers detected GUS expression in the tiller base (Fig. 4b,c), leaf-sheath pulvinus (Fig. 4d,e) and lamina joint (Fig. 4f), but not in the root, leaf blade and culm.We also observed GFP activity and found that PROG1 was expressed strongly in the vascular bundles of the leaf-sheath pulvinus (Fig. 4g,h). Consistent with the expression patterns shown by GUS staining, they detected higher expression of PROG1 in the leafsheath pulvinus, tiller base and lamina joint by real-time quantitative PCR (Fig. 4i); these organs might directly respond to the development and maintenance of plant structure

Evolution

You can also add sub-section(s) at will.

Labs working on this gene

  • 1State Key Laboratory of Plant Physiology and Biochemistry, National Center for Evaluation of Agricultural Wild Plants (Rice), Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100094, China.
  • MOE Key Laboratory of Bioinformatics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China.

References

  1. ↑ 1.0 1.1 1.2 Tan L, Li X, Liu F, Sun X, Li C, Zhu Z, Fu Y, Cai H, Wang X, Xie D, Sun C. Control of a key transition from prostrate to erect growth in rice domestication. Nat Genet. 2008 Nov;40(11):1360-4. doi: 10.1038/ng.197. PubMed PMID: 18820699.


Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os07g0153600&oldid=275867"