Difference between revisions of "Os03g0315400"

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Its gene name ''OsMYB2'', is a R2R3-type MYB gene, expression a MYB-type transcription factor plays a important role in the tolerance to abiotic stress of plants.   
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The rice '''''Os03g0315400''''' was reported as '''''OsMYB2''''' in 2012 <ref name="ref1" /> by researchers from China.  
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==Annotated Information==
 
==Annotated Information==
 +
===Gene Symbol===
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*'''''Os03g0315400''''' '''<=>''' '''''OsMYB2,MYB2'''''
 
===Function===
 
===Function===
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1、Overexpression of ''OsMYB2'' enhanced tolerance to salt cold, and dehydration stress（figure 1）.
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* '''''OsMYB2''''' encodes a stress-responsive MYB transcription factor.
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* '''''OsMYB2''''' plays a regulatory role in tolerance of rice to salt, cold, and dehydration stress.
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[[File:The tolerance to abiotic of overexpression and RNAi of OsMYB2.jpg]]
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===Phenotypic analysis===
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* No difference in growth and development between the OsMYB2-overexpressing and wild-type plants was observed under normal growth conditions, but the OsMYB2-overexpressing plants were more tolerant to salt, cold, and dehydration stresses and more sensitive to abscisic acid than wild-type plants.  
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The involvement of OsMYB2 in salt, cold, and osmotic
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* The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H 2 O 2 and malondialdehyde.  
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stress was investigated by exposing wild-type and the
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* The enhanced activities of antioxidant enzymes, including peroxidase, superoxide dismutase, and catalase, may underlie the lower H2O2 contents in OsMYB2-overexpressing plants.  
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transgenic plants grown in hydroponic solution with NaCl,
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* There was greater up-regulation of stress-related genes, including OsLEA3, OsRab16A, and OsDREB2A, in the OsMYB2-overexpressing plants. Microarray analysis showed that expression of numerous genes involving diverse functions in stress response was altered in the OsMYB2-overexpressing plants.
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low temperature, and PEG. There was no difference
 
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between transgenic and wild-type plants when grown under
 
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normal, non-stressed conditions in hydroponic solution
 
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(A). Phenotypically, most OsMYB2-overexpressing
 
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seedlings remained green and showed continuous growth,
 
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whereas both wild-type and RNAi seedlings showed severe
 
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leaf rolling and wilting after exposure to the salt, cold, and
 
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osmotic stress (B-D).In addition, the two transgenic
 
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rice lines overexpressing OsMYB2 grown in soil also
 
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exhibited greater tolerance to NaCl, cold, and drought
 
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stress than wild-type and RNAi plants（data not shown）
 
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WT:wildtype;
 
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OE2 and OE3:  ''OsMYB2'' overexpression strain;
 
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Ri1 and Ri3:  ''OsMYB2'' RNAi strain;
 
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2、Overexpression of ''OsMYB2'' altered sensitivity of seed germination and growth to salt stress and ABA(figure 2).
 
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[[File:Responses of seed germination and seedling growth to treatment with NaCl and abscisic acid (ABA).jpg]]
 
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This study also determined the survival rate for wild-type and
 
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transgenic plants grown in both hydroponic solution and
 
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soil challenged with salt, cold, and osmotic stress.the survival rate of overexpressing lines was
 
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significantly higher than that of wild-type and RNAi
 
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seedlings when exposed to salt stress (200 mM NaCl for
 
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2 d), cold stress (5 ℃ for 3 d), and osmotic stress (20%
 
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PEG6000 for 2 d). The survival rates of the two overexpressing
 
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lines higher than those of wild-type and RNAi
 
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plants were also observed when rice seedlings grown in
 
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soil were challenged by salt, cold, and drought stress（D-F）
 
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3、''OsMYB2''-overexpression plants accumulated greater amount of proline and soluble sugars(figure 3).
 
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[[File:Effect of salt stress on contents of proline and soluble sugars and gene expression in wild-type and transgenic rice plants.jpg]]
 
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Exposure of
 
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both wild-type and transgenic seeds to NaCl reduced their
 
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germination rate (A). However, germination of
 
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OsMYB2-overexpressing seeds was less inhibited by NaCl
 
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than that of wild-type and RNAi seeds. For example, seed
 
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germination rate of the two OsMYB2-overexpressing lines
 
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(OE2, OE3) was 81% and 86% when incubated in the
 
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presence of 100 mM NaCl, while germination rate for wildtype
 
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and RNAi (Ri1, Ri3) seeds was found to be 51%, 49%,
 
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and 53% under the identical conditions, respectively. The
 
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effect of NaCl on seedling growth was also examined. In
 
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the saline medium containing 150 mM NaCl, the OsMYB2-
 
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overexpressing plants exhibited faster growth and their
 
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shoots were significantly longer than wild-type plants
 
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(Fig. 6B, C).
 
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In contrast to salt stress, seed germination of OsMYB2-
 
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overexpressing lines was more sensitive to ABA than
 
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that of wild-type and RNAi lines, such that wild-type
 
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and RNAi lines had higher seed germination rate than
 
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Fig. 3. Molecular characterization and phenotypes of OsMYB2
 
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transgenic rice. (A) OsMYB2 expression in wild-type and transgenic
 
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rice. Total RNAs from 14-d-old wild-type and transgenic rice
 
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plants were isolated, reverse-transcribed, and analysed by realtime
 
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reverse-transcription PCR. Actin was used as an internal
 
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control. Error bars are based on three replicates. (B) The
 
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phenotypes of the T3 generation of wild-type and transgenic
 
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plants after growing on 1/2 MS medium for 14 days. (C) The
 
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phenotypes of the T3 generation of wild-type and transgenic
 
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plants after growing in soil for 30 days. Data are mean6SE of
 
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three biological replicates. Asterisks indicate statistically significant
 
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differences (P < 0.05) between wild-type (WT) and transgenic lines
 
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(OE and Ri).
 
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2546 | Yang et al.
 
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Downloaded from http://jxb.oxfordjournals.org/ at Institute of Biophysics,CAS on May 28, 2014
 
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OsMYB2-overexpressing lines when ABA was present in the
 
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incubation medium (D). Like seed germination, growth
 
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of OsMYB2-overexpressing seedlings was more inhibited by
 
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ABA than that of wild-type and RNAi seedlings, as shown by
 
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a shorter length of OsMYB2-overexpressing seedlings than
 
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wild-type and RNAi seedlings when grown in the presence of
 
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ABA (E, F). No difference in shoot length of wild-type
 
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and the transgenic plants grown in control medium was found.
 
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4、''OsMYB2''-overexpression plants accumulated less H2O2 and MDA under salt stress(figure 4).
 
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[[File:Effect of salt stress on contents of oxidants (A and B) and antioxidant enzymes (C–D) in wild-type and transgenic rice plants.jpg]]
 
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The effects of NaCl on H2O2 and MDA contents in wild-type
 
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and transgenic rice were investigated and no significant differences
 
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in H2O2 and MDA contents were found in the absence
 
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of NaCl in the incubation medium (A, B). There were
 
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marked increases in H2O2 and MDA contents in both wildtype
 
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and transgenic plants upon exposure to 200 mM NaCl.
 
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However, the salt stress-induced increases in H2O2 and MDA
 
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contents were less in the OsMYB2-overexpressing plants than
 
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those in the wild-type and RNAi plants. These results indicate
 
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that overexpression of OsMYB2 confers greater tolerance of
 
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the oxidative stress associated with salt stress.
 
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The lower content of H2O2 in the OsMYB2-overexpressing
 
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plants under salt stress prompted this study to test whether
 
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the difference in H2O2 accumulation between wild-type and
 
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the OsMYB2-overexpressing lines resulted from differences
 
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in the activities of the major antioxidant enzymes. Under
 
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normal conditions, activities of POD, SOD, and CAT were comparable among wild-type, OsMYB2-overexpressing,
 
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and RNAi plants (C-E). There were marked increases
 
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in activities of these enzymes for the rice seedlings upon
 
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exposure to salt stress. However, the salt stress-induced
 
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increases in activities of POD, SOD, and CAT were higher
 
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in the OsMYB2-overexpressing plants than the wild-type
 
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and RNAi plants (C-E). In contrast to OsMYB2-
 
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overexpressing plants, activities of these enzymes in the
 
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OsMYB2-RNAi lines did not differ from the wild-type
 
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plants under salt-stressed conditions (C-E). These
 
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results imply that overexpression of OsMYB2 confers
 
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a more efficient antioxidant system to counteract oxidative
 
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stress under saline conditions.
 
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5、''OsMYB2''-overexpression plants affect some genes' expression profiles. Some genes upregulation can contribute to enhanced tolerance of plants to salt stress and some may involved in stress response.  
 
  
 
===Expression===
 
===Expression===
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''OsMYB2'' was detected in roots,shoots,leaves, and flowers, but it was specifically located in the nucleus(figure 5)
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* Expression of '''''OsMYB2''''' was up-regulated by salt, cold, and dehydration stress.
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[[File:Subcellular localization of OsMYB2.jpg]]
 
[[File:Subcellular localization of OsMYB2.jpg]]
  
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==Labs working on this gene==
 
==Labs working on this gene==
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1 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093,
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* State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093,
 
PR China
 
PR China
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* Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China
  
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2 Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China
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==References==
 +
<references>
 +
* <ref name="ref1">
 +
Yang A, Dai X, Zhang WH. A R2R3-type MYB gene, OsMYB2, is involved in salt,
 +
cold, and dehydration tolerance in rice. J Exp Bot. 2012 Apr;63(7):2541-56. doi:
 +
10.1093/jxb/err431. PubMed PMID: 22301384; PubMed Central PMCID: PMC3346221.
 +
</ref>
  
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==References==
+
</references>
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A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice.An Yang1,2, Xiaoyan Dai1 and Wen-Hao Zhang*,1.Journal of Experimental Botany, Vol. 63, No. 7, pp. 2541–2556, 2012.
 
  
 
==Structured Information==
 
==Structured Information==

Revision as of 01:43, 14 February 2017

The rice Os03g0315400 was reported as OsMYB2 in 2012 [1] by researchers from China.

Annotated Information

Gene Symbol

  • Os03g0315400 <=> OsMYB2,MYB2

Function

  • OsMYB2 encodes a stress-responsive MYB transcription factor.
  • OsMYB2 plays a regulatory role in tolerance of rice to salt, cold, and dehydration stress.

Phenotypic analysis

  • No difference in growth and development between the OsMYB2-overexpressing and wild-type plants was observed under normal growth conditions, but the OsMYB2-overexpressing plants were more tolerant to salt, cold, and dehydration stresses and more sensitive to abscisic acid than wild-type plants.
  • The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H 2 O 2 and malondialdehyde.
  • The enhanced activities of antioxidant enzymes, including peroxidase, superoxide dismutase, and catalase, may underlie the lower H2O2 contents in OsMYB2-overexpressing plants.
  • There was greater up-regulation of stress-related genes, including OsLEA3, OsRab16A, and OsDREB2A, in the OsMYB2-overexpressing plants. Microarray analysis showed that expression of numerous genes involving diverse functions in stress response was altered in the OsMYB2-overexpressing plants.

Expression

  • Expression of OsMYB2 was up-regulated by salt, cold, and dehydration stress.

Subcellular localization of OsMYB2.jpg

a、b、c: localization of GFP ; e、d、f:localization of GFP-OsMYB2

The response of OsMYB2 expression to salt, cold, and dehydration stress was monitored by real-time RT-PCR. An increase in the OsMYB2 transcript was observed after 30 min of exposure to salt stress. The salt stress-induced increase in the OsMYB2 transcript peaked after 5 h of salt stress, and thereafter the transcript declined gradually under salt stress . A similar increase in the OsMYB2 transcript was also observed when rice seedlings were exposed to low temperature (2℃) or osmotic stress (20% PEG) . In addition, treatment of rice seedlings with ABA also led to an increase in expression of OsMYB2. In contrast, exogenous application of salicylic acid reduced the expression of OsMYB2, while no effect of indoleacetic acid and brassinosteroids on the OsMYB2 transcript was observed. OsMYB2 was detected in roots, shoots, leaves, and flowers under non-stressed conditions, with the expression being greatest in leaves, followed by roots and shoots . The strong induction of this gene by abiotic stress prompted this study to check its promoter sequence (1500 bp upstream from the transcription start site) by searching the promoter sequence against the PLACE database [(http://www.dna.affrc.go.jp/PLACE/)]. The promoter of OsMYB2 contains stress-responsive related cis-elements, such as ABRE and MYB and MYC recognition sites

Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg


Evolution

Phylogenetic tree of MYB proteins(figure 6).


Phylogenetic tree of MYB proteins.jpg

OsMYB2 representative by LOC_Os3g20090 is belong to C12, and of which are involved in stress response.

Labs working on this gene

  • State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093,

PR China

  • Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China

References

  1. ↑ Yang A, Dai X, Zhang WH. A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot. 2012 Apr;63(7):2541-56. doi: 10.1093/jxb/err431. PubMed PMID: 22301384; PubMed Central PMCID: PMC3346221.

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0315400&oldid=276196"