Difference between revisions of "Os03g0315400"

From RiceWiki
Jump to: navigation, search
(→‎Labs working on this gene)
 
(4 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
    
 
    
 
==Annotated Information==
 
==Annotated Information==
 +
[[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg|right|thumb|327px|'''Figure 2.''' ''Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.<ref name="ref1" />.'']]
 +
 
===Gene Symbol===
 
===Gene Symbol===
 
*'''''Os03g0315400''''' '''<=>''' '''''OsMYB2,MYB2'''''
 
*'''''Os03g0315400''''' '''<=>''' '''''OsMYB2,MYB2'''''
Line 9: Line 11:
 
===Phenotypic analysis===
 
===Phenotypic analysis===
 
* No difference in growth and development between the OsMYB2-overexpressing and wild-type plants was observed under normal growth conditions, but the OsMYB2-overexpressing plants were more tolerant to salt, cold, and dehydration stresses and more sensitive to abscisic acid than wild-type plants.  
 
* No difference in growth and development between the OsMYB2-overexpressing and wild-type plants was observed under normal growth conditions, but the OsMYB2-overexpressing plants were more tolerant to salt, cold, and dehydration stresses and more sensitive to abscisic acid than wild-type plants.  
−
* The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H 2 O 2 and malondialdehyde.  
+
* The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H2O2 and malondialdehyde.  
 
* The enhanced activities of antioxidant enzymes, including peroxidase, superoxide dismutase, and catalase, may underlie the lower H2O2 contents in OsMYB2-overexpressing plants.  
 
* The enhanced activities of antioxidant enzymes, including peroxidase, superoxide dismutase, and catalase, may underlie the lower H2O2 contents in OsMYB2-overexpressing plants.  
 
* There was greater up-regulation of stress-related genes, including OsLEA3, OsRab16A, and OsDREB2A, in the OsMYB2-overexpressing plants. Microarray analysis showed that expression of numerous genes involving diverse functions in stress response was altered in the OsMYB2-overexpressing plants.
 
* There was greater up-regulation of stress-related genes, including OsLEA3, OsRab16A, and OsDREB2A, in the OsMYB2-overexpressing plants. Microarray analysis showed that expression of numerous genes involving diverse functions in stress response was altered in the OsMYB2-overexpressing plants.
Line 15: Line 17:
 
===Expression===
 
===Expression===
 
* Expression of '''''OsMYB2''''' was up-regulated by salt, cold, and dehydration stress.
 
* Expression of '''''OsMYB2''''' was up-regulated by salt, cold, and dehydration stress.
−
[[File:Subcellular localization of OsMYB2.jpg]]
 
−
 
−
a、b、c: localization of GFP ;  e、d、f:localization of GFP-OsMYB2
 
−
 
−
The response of OsMYB2 expression to salt, cold, and
 
−
dehydration stress was monitored by real-time RT-PCR.
 
−
An increase in the OsMYB2 transcript was observed after
 
−
30 min of exposure to salt stress. The salt stress-induced
 
−
increase in the OsMYB2 transcript peaked after 5 h of salt
 
−
stress, and thereafter the transcript declined gradually under
 
−
salt stress . A similar increase in the OsMYB2
 
−
transcript was also observed when rice seedlings were
 
−
exposed to low temperature (2℃) or osmotic stress (20%
 
−
PEG) . In addition, treatment of rice seedlings with ABA
 
−
also led to an increase in expression of OsMYB2. In contrast,
 
−
exogenous application of salicylic acid reduced the expression of OsMYB2, while no effect of
 
−
indoleacetic acid and brassinosteroids on the OsMYB2
 
−
transcript was observed. OsMYB2 was detected in roots,
 
−
shoots, leaves, and flowers under non-stressed conditions,
 
−
with the expression being greatest in leaves, followed by
 
−
roots and shoots . The strong induction of this
 
−
gene by abiotic stress prompted this study to check its
 
−
promoter sequence (1500 bp upstream from the transcription
 
−
start site) by searching the promoter sequence against
 
−
the PLACE database [(http://www.dna.affrc.go.jp/PLACE/)].
 
−
The promoter of OsMYB2 contains stress-responsive related
 
−
cis-elements, such as ABRE and MYB and MYC
 
−
recognition sites
 
−
 
−
[[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg]]
 
  
 +
===Subcellular localization===
 +
* OsMYB2 was localized in the nucleus with transactivation activity.
 +
[[File:Subcellular localization of OsMYB2.jpg|center|thumb|727px|'''Figure 1.''' ''Subcellular localization and transactivation analysis of OsMYB2.<ref name="ref1" />.'']]
  
 
===Evolution===
 
===Evolution===
−
Phylogenetic tree of MYB proteins(figure 6).
+
* OsMYB2 representative by '''''LOC_Os3g20090''''' ('''''Os03g0315400''''') is belong to C12, and of which are involved in stress response.
−
 
 
−
 
 
−
[[File:Phylogenetic tree of MYB proteins.jpg]]
 
−
 
 
−
OsMYB2 representative by LOC_Os3g20090 is belong to C12, and of which are involved in stress response.
 
  
 
==Labs working on this gene==
 
==Labs working on this gene==

Latest revision as of 01:53, 14 February 2017

The rice Os03g0315400 was reported as OsMYB2 in 2012 [1] by researchers from China.

Annotated Information

Figure 2. Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.[1].

Gene Symbol

  • Os03g0315400 <=> OsMYB2,MYB2

Function

  • OsMYB2 encodes a stress-responsive MYB transcription factor.
  • OsMYB2 plays a regulatory role in tolerance of rice to salt, cold, and dehydration stress.

Phenotypic analysis

  • No difference in growth and development between the OsMYB2-overexpressing and wild-type plants was observed under normal growth conditions, but the OsMYB2-overexpressing plants were more tolerant to salt, cold, and dehydration stresses and more sensitive to abscisic acid than wild-type plants.
  • The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H2O2 and malondialdehyde.
  • The enhanced activities of antioxidant enzymes, including peroxidase, superoxide dismutase, and catalase, may underlie the lower H2O2 contents in OsMYB2-overexpressing plants.
  • There was greater up-regulation of stress-related genes, including OsLEA3, OsRab16A, and OsDREB2A, in the OsMYB2-overexpressing plants. Microarray analysis showed that expression of numerous genes involving diverse functions in stress response was altered in the OsMYB2-overexpressing plants.

Expression

  • Expression of OsMYB2 was up-regulated by salt, cold, and dehydration stress.

Subcellular localization

  • OsMYB2 was localized in the nucleus with transactivation activity.
Figure 1. Subcellular localization and transactivation analysis of OsMYB2.[1].

Evolution

  • OsMYB2 representative by LOC_Os3g20090 (Os03g0315400) is belong to C12, and of which are involved in stress response.

Labs working on this gene

  • State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, PR China
  • Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China

References

  1. ↑ 1.0 1.1 1.2 Yang A, Dai X, Zhang WH. A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot. 2012 Apr;63(7):2541-56. doi: 10.1093/jxb/err431. PubMed PMID: 22301384; PubMed Central PMCID: PMC3346221.

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os03g0315400&oldid=276203"