Difference between revisions of "Os01g0869200"
(→Expression) |
|||
| (6 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
| − | + | The rice '''''Os01g0869200''''' was reported as '''''OsABCC1''''' in 2014 <ref name="ref1" /> by researchers from Korea and Japan. | |
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:Example1234567.jpg|right|thumb|327px|'''Figure 1.''' ''Expression pattern of OsMGT1 in rice.<ref name="ref1" />.'']] | ||
===Function=== | ===Function=== | ||
| − | + | * Up-Regulation of a Magnesium Transporter Gene '''''OsMGT1''''' Is Required for Conferring Aluminum Tolerance in Rice. | |
| + | * '''''OsMGT1''''' is a transporter for Mg uptake in the roots and that up-regulation of this gene is required for conferring Al tolerance in rice by increasing Mg concentration in the cell. | ||
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * When a putative rice (Oryza sativa) Mg transporter gene, Oryza sativa MAGNESIUM TRANSPORTER1 (OsMGT1), was knocked out, the tolerance to Al, but not to cadmium and lanthanum, was decreased. | ||
| + | * However, this inhibition could be rescued by addition of 10 m M Mg, but not by the same concentration of barium or strontium. | ||
===Expression=== | ===Expression=== | ||
| − | + | * Real-time reverse transcription (RT)-PCR analysis revealed that OsMGT1 was expressed in both the shoots and roots at similar level in the absence of Al. | |
| − | + | * However, in the presence of Al, the expression in the roots was up-regulated by 5 times, but that in the shoots was unaffected. | |
| − | + | * Root spatial analysis showed that OsMGT1 was expressed in both the root tips (0–1 cm) and mature region (1–2 cm) and the expression in both regions were up-regulated by Al. | |
| − | + | * However, in art1 (a mutant defective in the Al response C2H2-type zinc-finger transcription factor), the Al-induced expression was not observed , confirming that OsMGT1 expression was regulated by ART1. | |
| − | |||
| − | |||
| − | |||
| − | + | ===Sequence Analysis === | |
| + | * OsMGT1 (Os01g0869200) cloned from rice (cv Nipponbare) contains six exons and five introns, encoding a peptide of 418 amino acids. | ||
| + | * Using the SOSUI program (http://bp.nuap.nagoya-u.ac.jp/sosui/), it has been predicted that OsMGT1 is a membrane protein with two transmembrane domains near the C terminus. | ||
| + | * OsMGT1 showed the similarity ranging from 63% to 81% to Arabidopsis Mg transporter AtMGT family at the amino acid level. Like other Arabidopsis members, there is a conserved Gly-Met-Asn tripeptide motif at the end of the first transmembrane. | ||
| − | + | ===Sub-cellular Localization of OsMGT1=== | |
| + | [[File:222-Os01g0869200.jpg|right|thumb|327px|'''Figure 3.''' ''Subcellular localization of OsMGT1.<ref name="ref1" />.'']] | ||
| + | * The subcellular localization of OsMGT1 was investigated in rice protoplasts by transiently expressing '''''OsMGT1''''' fused with GFP at both C terminal and N terminal under the control of 35S promoter. | ||
| + | * Either construct gave a signal at the plasma membrane , in contrast to GFP alone, which signal was observed in cytosol and nuclei.(http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/figure/fig3/) | ||
| − | |||
| − | |||
| − | |||
| − | |||
==Labs working on this gene== | ==Labs working on this gene== | ||
Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan (Z.C.C., N.Y., J.F.M.); and Genome Resource Center, Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305–8602, Japan (R.M., Y.N.) | Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan (Z.C.C., N.Y., J.F.M.); and Genome Resource Center, Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305–8602, Japan (R.M., Y.N.) | ||
| Line 30: | Line 35: | ||
Plant Physiology, 2012, 159(4): 1624-1633 | Plant Physiology, 2012, 159(4): 1624-1633 | ||
==Structured Information== | ==Structured Information== | ||
| − | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]] | |
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | [[Category:Genes]] | ||
| − | |||
| − | [[Category:Oryza Sativa Japonica Group]] | ||
| − | [[Category:Japonica | ||
| − | |||
| − | |||
Latest revision as of 09:46, 14 February 2017
The rice Os01g0869200 was reported as OsABCC1 in 2014 [1] by researchers from Korea and Japan.
Contents
Annotated Information
Figure 1. Expression pattern of OsMGT1 in rice.[1].
Function
- Up-Regulation of a Magnesium Transporter Gene OsMGT1 Is Required for Conferring Aluminum Tolerance in Rice.
- OsMGT1 is a transporter for Mg uptake in the roots and that up-regulation of this gene is required for conferring Al tolerance in rice by increasing Mg concentration in the cell.
Phenotypic analysis
- When a putative rice (Oryza sativa) Mg transporter gene, Oryza sativa MAGNESIUM TRANSPORTER1 (OsMGT1), was knocked out, the tolerance to Al, but not to cadmium and lanthanum, was decreased.
- However, this inhibition could be rescued by addition of 10 m M Mg, but not by the same concentration of barium or strontium.
Expression
- Real-time reverse transcription (RT)-PCR analysis revealed that OsMGT1 was expressed in both the shoots and roots at similar level in the absence of Al.
- However, in the presence of Al, the expression in the roots was up-regulated by 5 times, but that in the shoots was unaffected.
- Root spatial analysis showed that OsMGT1 was expressed in both the root tips (0–1 cm) and mature region (1–2 cm) and the expression in both regions were up-regulated by Al.
- However, in art1 (a mutant defective in the Al response C2H2-type zinc-finger transcription factor), the Al-induced expression was not observed , confirming that OsMGT1 expression was regulated by ART1.
Sequence Analysis
- OsMGT1 (Os01g0869200) cloned from rice (cv Nipponbare) contains six exons and five introns, encoding a peptide of 418 amino acids.
- Using the SOSUI program (http://bp.nuap.nagoya-u.ac.jp/sosui/), it has been predicted that OsMGT1 is a membrane protein with two transmembrane domains near the C terminus.
- OsMGT1 showed the similarity ranging from 63% to 81% to Arabidopsis Mg transporter AtMGT family at the amino acid level. Like other Arabidopsis members, there is a conserved Gly-Met-Asn tripeptide motif at the end of the first transmembrane.
Sub-cellular Localization of OsMGT1
File:222-Os01g0869200.jpg
Figure 3. Subcellular localization of OsMGT1.[1].
- The subcellular localization of OsMGT1 was investigated in rice protoplasts by transiently expressing OsMGT1 fused with GFP at both C terminal and N terminal under the control of 35S promoter.
- Either construct gave a signal at the plasma membrane , in contrast to GFP alone, which signal was observed in cytosol and nuclei.(http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425201/figure/fig3/)
Labs working on this gene
Institute of Plant Science and Resources, Okayama University, Kurashiki 710–0046, Japan (Z.C.C., N.Y., J.F.M.); and Genome Resource Center, Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305–8602, Japan (R.M., Y.N.)
References
1. Zhi Chang Chen;Naoki Yamaji;Ritsuko Motoyama;Yoshiaki Nagamura;Jian Feng Ma
Up-Regulation of a Magnesium Transporter Gene OsMGT1 Is Required for Conferring Aluminum Tolerance in Rice Plant Physiology, 2012, 159(4): 1624-1633
