Difference between revisions of "Os01g0776600"
(Created page with "{{JaponicaGene|
GeneName = Os01g0776600|
Description = Similar to Purple acid phosphatase (EC 3.1.3.2)|
Version = NM_001050951.1 GI:115440272 GeneID:4327894|
Length = 4254...") |
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| − | + | The rice '''''Os01g0776600''''' was reported as '''''OsPAP10a''''' in 2012 <ref name="ref1" /> by researchers from China. | |
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| − | + | ==Annotated Information== | |
| − | + | ===Function=== | |
| − | + | [[File:225-Os01g0776600.png|right|thumb|327px|'''Figure 1.''' ''Growth of WT and transgenic plants cultured in soil pots.<ref name="ref1" />.'']] | |
| − | + | * The rice (Oryza sativa) purple acid phosphatase 10a (OsPAP10a) belongs to group Ia of purple acid phosphatases (PAPs), and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis. | |
| − | + | * '''''OsPAP10a''''' can potentially be used for crop breeding to improve the efficiency of P use. | |
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| − | + | ===Phenotypic analysis=== | |
| − | + | * Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root-associated APase. | |
| − | + | * Furthermore, overexpression of '''''OsPAP10a''''' significantly improved ATP hydrolysis and utilization compared with wild type plants. | |
| − | + | ||
| − | + | ===Expression=== | |
| − | + | * The transcript abundance of '''''OsPAP10a''''' is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation. | |
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| − | + | ===Evolution=== | |
| − | + | * In plants, the PAP family has been previously classified into seven groups and OsPAP10abelongs to group Ia. In rice, group Ia contains 5 members, namely OsPAP10a to OsPAP10d, and OsPAP26. | |
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| − | + | ==Labs working on this gene== | |
| − | < | + | * State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China |
| − | + | * Research Laboratory in Genomics and Nutriomics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China | |
| − | + | * Present address: Guangdong Academy of Forestry, Guangzhou 510520, China | |
| − | + | * Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley 6009, WA, Australia | |
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| − | + | ==References== | |
| − | [[Category:Genes]] | + | <references> |
| − | + | * <ref name="ref1"> | |
| − | [[Category:Oryza Sativa Japonica Group]] | + | Tian J, Wang C, Zhang Q, He X, Whelan J, Shou H. Overexpression of OsPAP10a, a |
| − | + | root-associated acid phosphatase, increased extracellular organic phosphorus | |
| − | [[Category:Japonica | + | utilization in rice. J Integr Plant Biol. 2012 Sep;54(9):631-9. doi: |
| − | + | 10.1111/j.1744-7909.2012.01143.x. PubMed PMID: 22805094. | |
| + | </ref> | ||
| + | |||
| + | ==Structured Information== | ||
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 1]] | ||
Latest revision as of 14:21, 14 February 2017
The rice Os01g0776600 was reported as OsPAP10a in 2012 [1] by researchers from China.
Contents
Annotated Information
Function
Figure 1. Growth of WT and transgenic plants cultured in soil pots.[1].
- The rice (Oryza sativa) purple acid phosphatase 10a (OsPAP10a) belongs to group Ia of purple acid phosphatases (PAPs), and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis.
- OsPAP10a can potentially be used for crop breeding to improve the efficiency of P use.
Phenotypic analysis
- Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root-associated APase.
- Furthermore, overexpression of OsPAP10a significantly improved ATP hydrolysis and utilization compared with wild type plants.
Expression
- The transcript abundance of OsPAP10a is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation.
Evolution
- In plants, the PAP family has been previously classified into seven groups and OsPAP10abelongs to group Ia. In rice, group Ia contains 5 members, namely OsPAP10a to OsPAP10d, and OsPAP26.
Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China
- Research Laboratory in Genomics and Nutriomics, College of Life Sciences, Zhejiang University, Hangzhou 310058, China
- Present address: Guangdong Academy of Forestry, Guangzhou 510520, China
- Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley 6009, WA, Australia
References
<references>
