Difference between revisions of "Os11g0690066"
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| − | + | The rice '''''Os11g0690066''''' was reported as '''''OsWAK1''''' in 2009 <ref name="ref1" /> by researchers from China. | |
| − | |||
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==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | [[File:229-Os11g0690066.png|right|thumb|327px|'''Figure 3.''' ''Subcellular localization of OsWAK1 protein.<ref name="ref1" />.'']] | ||
| + | *'''''Os11g0690066''''' '''''<=>''''' '''''OsWAK1''''','''''WAK1''''' | ||
===Function=== | ===Function=== | ||
| − | + | * '''''OsWAK1''''' not only has the ability of autophosphorylation but also can phosphorylate OsRFP1, a putative transcription regulator recently identified in rice. OsRFP1 strongly interacts with the kinase domain of OsWAK1. This demonstrated that OsWAK1 is a functional protein kinase. | |
| + | * Plasmolysis experiments further revealed '''''OsWAK1''''' is associated with the cell wall. | ||
| + | * '''''OsWAK1''''' is a novel gene involved in plant defense. | ||
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * Six transgenic rice lines with constitutive expression of '''''OsWAK1''''' became resistant to the compatible race. However, '''''OsWAK1''''' expression was undetectable in leaves, stems and flowers but very weak in roots under normal growth conditions. | ||
| + | * This provides functional evidence that induction of OsWAK1 as a novel RLK plays important roles in plant disease resistance. | ||
===Expression=== | ===Expression=== | ||
| − | + | * Northern blotting analysis showed that infection of the rice blast fungus, Magnaporthe oryzae significantly induced the '''''OsWAK1''''' transcripts, and the accumulation of '''''OsWAK1''''' mRNA occurred earlier and was more abundant in rice leaves infected with an incompatible race than with a compatible race of the blast fungus. | |
| + | * '''''OsWAK1''''' was also induced after treatment by mechanical wounding, SA and MeJA, but not by ABA. | ||
===Evolution=== | ===Evolution=== | ||
| − | + | * The deduced amino acid sequence of OsWAK1 showed 27.6% identity to WAK2 from Arabidopsis. | |
| + | |||
| + | ===Subcellular localization=== | ||
| + | * A fusion protein of OsWAK1 with GFP was found to be localized on the cell surface. | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * State Key Laboratory for Agrobiotechnology and the Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China | |
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Li H, Zhou SY, Zhao WS, Su SC, Peng YL. A novel wall-associated receptor-like | ||
| + | protein kinase gene, OsWAK1, plays important roles in rice blast disease | ||
| + | resistance. Plant Mol Biol. 2009 Feb;69(3):337-46. doi: | ||
| + | 10.1007/s11103-008-9430-5. PubMed PMID: 19039666. | ||
| + | </ref> | ||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
[[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 11]] | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 11]] | ||
Latest revision as of 15:53, 14 February 2017
The rice Os11g0690066 was reported as OsWAK1 in 2009 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
Figure 3. Subcellular localization of OsWAK1 protein.[1].
- Os11g0690066 <=> OsWAK1,WAK1
Function
- OsWAK1 not only has the ability of autophosphorylation but also can phosphorylate OsRFP1, a putative transcription regulator recently identified in rice. OsRFP1 strongly interacts with the kinase domain of OsWAK1. This demonstrated that OsWAK1 is a functional protein kinase.
- Plasmolysis experiments further revealed OsWAK1 is associated with the cell wall.
- OsWAK1 is a novel gene involved in plant defense.
Phenotypic analysis
- Six transgenic rice lines with constitutive expression of OsWAK1 became resistant to the compatible race. However, OsWAK1 expression was undetectable in leaves, stems and flowers but very weak in roots under normal growth conditions.
- This provides functional evidence that induction of OsWAK1 as a novel RLK plays important roles in plant disease resistance.
Expression
- Northern blotting analysis showed that infection of the rice blast fungus, Magnaporthe oryzae significantly induced the OsWAK1 transcripts, and the accumulation of OsWAK1 mRNA occurred earlier and was more abundant in rice leaves infected with an incompatible race than with a compatible race of the blast fungus.
- OsWAK1 was also induced after treatment by mechanical wounding, SA and MeJA, but not by ABA.
Evolution
- The deduced amino acid sequence of OsWAK1 showed 27.6% identity to WAK2 from Arabidopsis.
Subcellular localization
- A fusion protein of OsWAK1 with GFP was found to be localized on the cell surface.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory for Agrobiotechnology and the Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China
