Difference between revisions of "Os10g0536100"

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===Function===
 
===Function===
 
* '''''OsMADS50''''' and '''''OsMADS56''''' may form a complex that regulates down- stream target genes.
 
* '''''OsMADS50''''' and '''''OsMADS56''''' may form a complex that regulates down- stream target genes.
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===Phenotypic analysis===
 
===Phenotypic analysis===
 
* Overexpression of OsMADS56 (56OX) resulted in delayed flowering under LD.
 
* Overexpression of OsMADS56 (56OX) resulted in delayed flowering under LD.
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* Yeast two-hybrid and co-immunoprecipitation analyses indicated an interaction between those two proteins as well as their formation of homodimers.
 
* Yeast two-hybrid and co-immunoprecipitation analyses indicated an interaction between those two proteins as well as their formation of homodimers.
 
                    
 
                    
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===Expression===
 
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(1)Using the maize Ubiquitin promoter in transgenic plants that over-expressed OsMADS56 (56OX; Fig. 2a). Among 17 primary (T1) plants, 9 flowered late by 1–2 weeks in the paddy field. RT-PCR analyses of three normal plants (#4, 19 and 20) and five late-flowering plants (#6, 8, 10, 11 and 13) demonstrated that the extent of late flowering was correlated with OsMADS56 transcript levels (data not shown). Then selected Lines 8 and 10 for further analyses of the next generations when grown in controlled environments. Both lines displayed a dominant late-flowering phenotype under LDs. Homozygous progeny flowered late
 
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by 3–4 weeks under LD (Fig. 2b,c), although no significant change was observed under SD (Fig. 2d). These results suggest that OsMADS56 is an LD-specific floral repressor. They observed no other phenotypic changes in this overexpressor except for the delayed-flowering phenotypes.
 
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[[File:fig2b.jpg]] [[File:fig2c.jpg]] [[File:fig2d.jpg]]
 
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(2)Real-time RT-PCR analyses indicates that OsMADS56 transcript levels were low at the seedling stage but gradually increased to a maximum at 4 weeks after
 
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germination as the observation made by CHOONG-HWAN RYU1 etc. (fig.3b)
 
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(3)OsMADS56 and OsMADS50 control the Rice's flowering time through regulating their communal downstream genes OsLFL1-Ehd1 .The expression patterns of Ehd1, Hd3a RFT1 and OsLFL1 reveal it.(fig3c-f)
 
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(4)The mRNA levels of OsMADS50 and OsMADS56 along with other flowering regulators such as Ehd1, Hd1 reveals that OsMADS50 and OsMADS56 also have daily rhythms.(fig9) The transcript levels of them is similar to that of Ehd1.
 
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(5)OsMADS56 is located on chromosome 10, and consists of eight exons that encode a typical MIKC-type MADS-box protein.
 
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  [[File:f3b-f.jpg]][[File:fig9.jpg]]
 
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===Evolution===
 
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In those Ehd1-RNAi plants, mRNA levels of both OsMADS56 and OsMADS50 were unchanged,indicating that OsMADS50 and OsMADS56 are upstream of Ehd1.Transcript levels of OsMADS56 were not altered in OsID1-RNAi plants , indicating that OsMADS56 is not hypostatic to OsID1. The level of Hd1 transcript was not affected in osmads50mutant and 56OX transgenic plants. T-DNA insertional knockdown plants for Hd1 reveals that mRNA levels of these two MADS genes in the mutants were unaltered under both SD and LD conditions,thus the LD-flowering pathway mediated by these two MADS genes is independent of Hd1.
 
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=== Knowledge Extension ===
 
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By conducting Co-IP experiments, they confirm the physical interaction between OsMADS50 and OsMADS56. Myc-tagged OsMADS50 was first co-transfected with HA-tagged OsMADS56 into rice protoplasts before whole-protoplast extracts expressing epitope-tagged proteins were immunoprecipitated with anti-Myc antibodies. Immunoblot analysis with anti-HA antibodies revealed that HA-tagged OsMADS56 was co-precipitated with the Myc-tagged OsMADS50 (Fig. 8b). Similarly, HA-tagged OsMADS50 was co-precipitated with the Myc-tagged OsMADS56 (Fig. 8b). However, neither OsMADS50-HA nor OsMADS56-Myc alone showed such co-precipitation.These experiments supported our findings from the yeast two-hybrid trials that OsMADS50 and OsMADS56 do interact with each other. We also showed that OsMADS50 and OsMADS56 form homodimers, based on those yeast-two hybrid (data not shown) and Co-IP experiments (Fig. 8c).
 
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[[File:fig8a.jpg]] [[File:fig8bc.jpg]]
 
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==Labs working on this gene==
 
==Labs working on this gene==

Latest revision as of 01:34, 17 February 2017

The rice Os10g0536100 was reported as OsMADS56 in 2009 [1] by researchers from Korea.

Annotated Information

Figure 2. Gene structure of Os10g0536100 .[1].

Gene Symbol

  • Os10g0536100 <=> OsMADS56,MADS56,RMADS214

Function

  • OsMADS50 and OsMADS56 may form a complex that regulates down- stream target genes.

Phenotypic analysis

  • Overexpression of OsMADS56 (56OX) resulted in delayed flowering under LD.
  • In the osmads50 mutants and 56OX transgenic plants, transcripts of Ehd1, Hd3a and RFT1 were reduced, although that of OsLFL1 increased.
  • On the other hand, mRNA levels of OsGI, Hd1, OsId1, OsDof12, Ghd7, Hd6 and SE5 were unchanged.
  • These observations imply that OsMADS50 and OsMADS56 function antagonistically through OsLFL1-Ehd1 in regulating LD-dependent flowering.
  • Yeast two-hybrid and co-immunoprecipitation analyses indicated an interaction between those two proteins as well as their formation of homodimers.

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Labs working on this gene

  • CHOONG-HWAN RYU1 ,Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea
  • SOON JU PARK2&CHANG-DEOK HAN, Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju 660-701, Korea

References

  1. ↑ 1.0 1.1 Ryu CH, Lee S, Cho LH, Kim SL, Lee YS, Choi SC, Jeong HJ, Yi J, Park SJ, Han CD, An G. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)-dependent flowering in rice. Plant Cell Environ. 2009 Oct;32(10):1412-27. doi: 10.1111/j.1365-3040.2009.02008.x. PubMed PMID: 19558411.

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os10g0536100&oldid=276375"