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| | ===Function=== | | ===Function=== |
| − | ''OsPIL11'' is a member of the rice phytochrome-interacting factors (PIFs) family. Nakamura et al <ref name="ref2" /> identified six candidate genes encoding PIFs, designated ''OsPIL11'' to ''OsPIL16'', via homologous analysis in rice genome. ''OsPIL11'' contain APB motif at their N-termini, suggesting the possible interaction between PIF and phytochromes in rice <ref name="ref2" />. | + | ''OsPIL11'' is a member of the rice phytochrome-interacting factors (PIFs) family. Nakamura et al identified six candidate genes encoding PIFs, designated ''OsPIL11'' to ''OsPIL16'', via homologous analysis in rice genome. ''OsPIL11'' contain APB motif at their N-termini, suggesting the possible interaction between PIF and phytochromes in rice . |
| − | PIFs, as a small subset of the basic helix-loop-helix (bHLH) transcription factor superfamily, have been found to bind to the G-box motif in the promoter region of light-regulated genes. Thus, PIFs constitute a signal transfer pathway from photoactivated phytochromes to the light-regulation of gene expression that controls photomorphogenesis in plants. Among phytochrome associated proteins, phytochrome-interacting factors (PIFs) are central player in phytochrome-mediated signal transduction <ref name="ref3" /><ref name="ref4" />. | + | |
| | + | PIFs, as a small subset of the basic helix-loop-helix (bHLH) transcription factor superfamily, have been found to bind to the G-box motif in the promoter region of light-regulated genes. Thus, PIFs constitute a signal transfer pathway from photoactivated phytochromes to the light-regulation of gene expression that controls photomorphogenesis in plants. Among phytochrome associated proteins, phytochrome-interacting factors (PIFs) are central player in phytochrome-mediated signal transduction. |
| | In addition, ''OsPIL11'' plays important roles in light signal transduction in rice leaves and its development, and it may also involve in the regulation of plant hormones. | | In addition, ''OsPIL11'' plays important roles in light signal transduction in rice leaves and its development, and it may also involve in the regulation of plant hormones. |
| | | | |
| | ===Expression=== | | ===Expression=== |
| | ''OsPIF11'' gene is widely expressed in rice roots, stems, new leaves and old leaves. The ''OsPIFs11'' transcription factor expression level in the leaves is significantly higher than in the roots and stems, and the expression is the lowest in roots. | | ''OsPIF11'' gene is widely expressed in rice roots, stems, new leaves and old leaves. The ''OsPIFs11'' transcription factor expression level in the leaves is significantly higher than in the roots and stems, and the expression is the lowest in roots. |
| − | The expression of ''OsPIL11'' also is organ-specific and is regulated by leaf development, abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). ''OsPIL11'' is involved in red light-induced de-etiolation, but not in far-red lignt-induced de-etiolation in transgenic tobacco. ''OsPIL11'' expressed higher in the new leaves than in the old leaves. Therefore, the expression of ''OsPIL11'' gene was regulated by hormones in rice <ref name="ref5" />. | + | |
| | + | The expression of ''OsPIL11'' also is organ-specific and is regulated by leaf development, abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). ''OsPIL11'' is involved in red light-induced de-etiolation, but not in far-red lignt-induced de-etiolation in transgenic tobacco. ''OsPIL11'' expressed higher in the new leaves than in the old leaves. Therefore, the expression of ''OsPIL11'' gene was regulated by hormones in rice. |
| | | | |
| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | ·High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
| + | * High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China |
| − | ·College of Life Science, Henan Normal University, Xinxiang 453007, China
| + | |
| − | ·College of Life Sciences, Shandong Normal University, Jinan 250014, China
| + | * College of Life Science, Henan Normal University, Xinxiang 453007, China |
| − | ·Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan
| + | |
| − | ·National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, ·Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
| + | * College of Life Sciences, Shandong Normal University, Jinan 250014, China |
| | + | |
| | + | * Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan |
| | + | |
| | + | * National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China |
| | | | |
| | ==References== | | ==References== |
| | <references> | | <references> |
| − | <ref name="ref1">Khanna R, Huq E, Kikis E A, Al-Sady B, Lanzatella C, Quail P H. 2004. A novel molecular recognition motif necessary for targeting photoactivated phytochrome signaling to specific basic helix-loop-helix transcription factors. Plant Cell, 16:3033-3044.</ref> | + | * <ref name="ref1"> |
| − | <ref name="ref2">Nakamura Y, Kato Y, Yamashino Y, Murakami M, Mizuno T. 2007. Characterization of a set of phytochrome-interacting-facor-like bHLH proteins in Oryza sativa. Biosci Biotechnol Biochem, 71:1183-1191.</ref>
| + | Nakamura Y, Kato T, Yamashino T, Murakami M, Mizuno T. Characterization of a |
| − | <ref name="ref3">Zhao X L. 2009. Phytochrome-interacting factors (PIFs) in plant. Plant Physiol Comm, 45:531-536.</ref>
| + | set of phytochrome-interacting factor-like bHLH proteins in Oryza sativa. Biosci |
| − | <ref name="ref4">Leivar P, Quail P H. 2011. PIFs: Pivotal components in a cellular signaling hub. Trends Plant Sci, 16:19-28.</ref>
| + | Biotechnol Biochem. 2007 May;71(5):1183-91. PubMed PMID: 17485859. |
| − | <ref name="ref5">Li L, Peng WF, Liu QQ, Zhou JJ, Liang WH, Xie XZ. 2012. Expression Patterns of OsPIL11, a Phytochrome-Interacting Factor in Rice, and Preliminary Analysis of Its Roles in Light Signal Transduction. Rice Science, 19(4):263-268.</ref>
| + | </ref> |
| − | | + | </references> |
| − | {{JaponicaGene|
| |
| − | GeneName = Os12g0610200|
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| − | Description = Similar to Phytochrome-interacting factor 3 (Phytochrome-associated protein 3) (Basic helix-loop-helix protein 8) (bHLH8) (AtbHLH008)|
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| − | Version = NM_001073778.1 GI:115489517 GeneID:4352761|
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| − | Length = 4245 bp|
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| − | Definition = Oryza sativa Japonica Group Os12g0610200, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| | | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
| |
| − | Chromosome = [[:category:Japonica Chromosome 12|Chromosome 12]]|
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| − | AP = Chromosome 12:25995255..25999499|
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| − | CDS = 25996330..25996391,25996490..25997264,25997440..25997505,25997637..25997702,25997789..25998031<br>,25998268..25998393|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008405:25995255..25999499
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| − | source=RiceChromosome12
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| − | preset=GeneLocation
| |
| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
| |
| − | name=NC_008405:25995255..25999499
| |
| − | source=RiceChromosome12
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atgaaccagttcgtccctgattggaacaccaccagcatgggcgacggctttgcgccattaggcgaagacgacgggctcgtcgagctgctatggtgcaatggccacgtcgtcatgcagagccaggcgccgcggaagccgccgaggccggagaagacgacggcggcggcggcggcggcgatggcggaggatgagtcggcgtcgtggtttcagtacccggtcgacgacgtgcttgagaaggacctgttcaccgagctgttcggcgaaatgacggcggccggcggcggcggcggcgacgtccgcagggcggcgtgcaaggaggagcgcggcgcggtcgccgcgttccagagcaggatgatgccgccgccgtggccggcgagggggaaggcggagttcggtgacgtcgacgacgtgtgcggcgtctcggaggtcgtcatggcgaagatggacggggcggcggcggcggagacggtcggcgagtcatcgatgctgacaatcgggtcgagcatctgcgggagcaaccacgtccagacgccgccggtggggaacgggaaggccggcgccggcaccgccggcgccgccagaagggcgcacgacacggcgacggtggcgtcgtcgtcgatgaggtcgaggtcctgcaccgccaaggccgagccgcgcgacgtcgcagccgccggcgtcggcggcaagcggaagcagcgcggcggcgccgccatggagtccgggagccccagcgaggacgtggagttcgagtccgccgccgcaacgtgctcgccggcgcagaagacgacgacggcgaagcggcggcgcgccgccgaggtgcacaacctctccgagaggaggagaagagataggatcaatgagaagatgaaagcattacaggagctcatacctcactgcaacaaaacggacaaagcatcgatgctggatgaagcgatcgagtatctcaagtcactgcagctccagctacagatgatgtggatgggcggcggaatggcgccgccggcggtgatgttcccggcggccggcgtgcaccagtacatgcagcggatgggcgccgtcgggatgggcccaccacacatggcgtccctgccgaggatgccgccgttcatggcgccgccgcccgccgccgtgcagagctcgccggtggtcagcatggccgacccctacgcccgctgcctcgccgtcgaccacctccagccaccgcctccgatgcattacctgcaggggatgagcttctaccagctcgccgcggccaagaaccttcagcagcagcagaacacggcggaggcgccgccaccgccaccggccggaggcaaccgcgcagccgctgactcctga</cdnaseq>|
| |
| − | AA = <aaseq>MNQFVPDWNTTSMGDGFAPLGEDDGLVELLWCNGHVVMQSQAPR KPPRPEKTTAAAAAAMAEDESASWFQYPVDDVLEKDLFTELFGEMTAAGGGGGDVRRA ACKEERGAVAAFQSRMMPPPWPARGKAEFGDVDDVCGVSEVVMAKMDGAAAAETVGES SMLTIGSSICGSNHVQTPPVGNGKAGAGTAGAARRAHDTATVASSSMRSRSCTAKAEP RDVAAAGVGGKRKQRGGAAMESGSPSEDVEFESAAATCSPAQKTTTAKRRRAAEVHNL SERRRRDRINEKMKALQELIPHCNKTDKASMLDEAIEYLKSLQLQLQMMWMGGGMAPP AVMFPAAGVHQYMQRMGAVGMGPPHMASLPRMPPFMAPPPAAVQSSPVVSMADPYARC LAVDHLQPPPPMHYLQGMSFYQLAAAKNLQQQQNTAEAPPPPPAGGNRAAADS</aaseq>|
| |
| − | DNA = <dnaseqindica>1076..1137#1236..2010#2186..2251#2383..2448#2535..2777#3014..3139#gctctccggcgagtcctcttcctgccctgccctgccctgccctgcattctttctttctccaccaggggaatccagttcacccccagtgctgcttctgctgctgcttctgcatcatcttgccctgttaaaaagacacagtgcccttgttctttcgcagttgcaactagcatctcctcctctacttgtactcacttcacacctcagctcagctcagctcatctcctgtcatctcagctcaaagagaaagagctgaaggtgtaagctgatcaccaggaagcagaggctttttttcagattacagttatctgaaacaaccaacttcagaatcaatcagcaaaggtagagggagtttcttgtttcttttgtgtactactacatgactatatccatatctcctttttcttctacttctttttcttttgtttggttgtgagtgttgttgctgtgtgtttcaacaagtcaactggatagatagattcatagatatgatagtttttatctgaggttggaggtttcccctctcatccaccacacacattttggcaccccaatcctgaatttactgttcaattctcttctttttattcttcctctgttgattgatcatccatgcatgcatactggtatatagtactagtgctcaagtgctcatccttcctgatggtgaatctcttttgagaaaagaaaagaaaaagatctctctcatcttggctttttttcctgttgatctgagcactgctcttctccaggttgtttcattctgcaggtctacagcttttgtcatcaaagtggtagggtttgtactttgtagtagattgcagtgatggcccttctctgcttttttttcattctctgtttgttcttgttgctcttattctgtttcattctactttttttaaacaaatccatccttagagttcagttttcccttaaaatttttccaaacctacaaattttgttctctgtgaattcatttttgcatgattcttgcagaatctgaaatgatcattggctttgcaggtagaaacaagacagagctgctgtgcttctgtgattaattagggttgttaatgccatgaaccagttcgtccctgattggaacaccaccagcatgggcgacggctttgcgccattagggttcgtgcgattcttggatcgaagcttgagatctttccatcgattggaggcgatcggatgagtgattctttggtttcttgttcttcttgttgttgcagcgaagacgacgggctcgtcgagctgctatggtgcaatggccacgtcgtcatgcagagccaggcgccgcggaagccgccgaggccggagaagacgacggcggcggcggcggcggcgatggcggaggatgagtcggcgtcgtggtttcagtacccggtcgacgacgtgcttgagaaggacctgttcaccgagctgttcggcgaaatgacggcggccggcggcggcggcggcgacgtccgcagggcggcgtgcaaggaggagcgcggcgcggtcgccgcgttccagagcaggatgatgccgccgccgtggccggcgagggggaaggcggagttcggtgacgtcgacgacgtgtgcggcgtctcggaggtcgtcatggcgaagatggacggggcggcggcggcggagacggtcggcgagtcatcgatgctgacaatcgggtcgagcatctgcgggagcaaccacgtccagacgccgccggtggggaacgggaaggccggcgccggcaccgccggcgccgccagaagggcgcacgacacggcgacggtggcgtcgtcgtcgatgaggtcgaggtcctgcaccgccaaggccgagccgcgcgacgtcgcagccgccggcgtcggcggcaagcggaagcagcgcggcggcgccgccatggagtccgggagccccagcgaggacgtggagttcgagtccgccgccgcaacgtgctcgccggcgcagaagacgacgacggcgaagcggcggcgcgccgccgaggtgcacaacctctccgagagggtacgtgtcatgcacgctcacattttgttcttcttgctttgacttttctctcttattattattattattattattattattattattattattattattattattattattattattattattattattattgctgctgctgctaataaaaaatgttaaattgattgtgatgcagaggagaagagataggatcaatgagaagatgaaagcattacaggagctcatacctcactgcaacaaagtaaactactctaaatttaatctgcaagcgccattacataattacaactagcaacattgctatacatctcattaaaatttaaatcgaatttatttgaaaatggtgacctcattcttgtttcatattttcagacggacaaagcatcgatgctggatgaagcgatcgagtatctcaagtcactgcagctccagctacaggtttgtactacgtaaaatgaatgtaactcaatactaaaattgattaacgataatccgacgatgatgatgtgaatggcgtggttcagatgatgtggatgggcggcggaatggcgccgccggcggtgatgttcccggcggccggcgtgcaccagtacatgcagcggatgggcgccgtcgggatgggcccaccacacatggcgtccctgccgaggatgccgccgttcatggcgccgccgcccgccgccgtgcagagctcgccggtggtcagcatggccgacccctacgcccgctgcctcgccgtcgaccacctccagccaccgcctccgatggtaagtaaagcacgtgcctgtacagtcaccatgcatcgatcagtaattcagtagctgccatgaaatgtattttcagtttcgacgcgaatactagggaaggaactaatatcaaataatagaaggggtgagccttcgaatcgagatcgtctagcccaccaccttatagagctagccggaaggccctcgagcgtttctcatattttcagtttcctaagagtttttttttttgtcagcagcattacctgcaggggatgagcttctaccagctcgccgcggccaagaaccttcagcagcagcagaacacggcggaggcgccgccaccgccaccggccggaggcaaccgcgcagccgctgactcctgacgacattctgcacaaaaaatacggtgtgtaagtactatctactccacatttcagtgtcaatgtacatgaaacaaaaatgaaaatcagtgatctgctactagtctactagtagtactagtatcgtaccacaaagaatgcttcaaggcctgaaatttcgacccaaatacccgaaatttccgggttccgatcctcgtacaagtactacatatgtcctaaaatataacaacttttacctatgaatctagccaagtgcatgtctagattcgtagtcaaaagttaatgtattttaggatggatgaattatgaaatttcggaacgaaatacctgagaattcgggggtattgatcctccagtagtactatatgttggaaaagaaaaatcagacagaataaatatatgcattttttcgcttgaaattttatttgaaattgccctgtaaaatgagccagaaaagtctaaggaatttaaagatgaataaaatctgagggtgtgaacacaggtttgctttcagcaaagacaatgtgatctggttagctcaagaacctgtcattgtcatgcactgtaggctagagtttactttgctgatttcactcggctacatgcactgcatccatcggtgtcaaattcacggactagttgatcatttatctcttgtagatccgtggatagctgcatcacagaaactctgaatttctctgttcttaaatgcaactgtttgttcatctctgacttgacttatcactcacatatctgtctcttttttgtccctgaaatattgcagacaactgtagtaagtctgagagcaagggtggcaccagttgattgccgtcaatctgaagatatgtatataaggctctgatgcaattgagttgagaggctggatcatgatggctacctaatcattaaggggaatttaagatcatcccttttactgacgtctaaattcggtaggcagtgtagatcgatcagcggttaagagttaacaagtcgtgaattcttttttgctatgtatgtagtactattttttcccctttggggagacattggttatgtatcagttgttgatgtacctgttcaaatttatgccaaccagagcaaattgtatt</dnaseqindica>|
| |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001073778.1 RefSeq:Os12g0610200]|
| |
| − | }}
| |
| | [[Category:Genes]] | | [[Category:Genes]] |
| | [[Category:Japonica mRNA]] | | [[Category:Japonica mRNA]] |
| Line 67: |
Line 43: |
| | [[Category:Japonica Chromosome 12]] | | [[Category:Japonica Chromosome 12]] |
| | [[Category:Chromosome 12]] | | [[Category:Chromosome 12]] |
| | + | |
| | + | ==Structured Information== |
OsPIL11, with accession number Os12g0610200, is one of six putative phytochrome-interacting factors(PIFs).
Annotated Information
Constitution
OsPIL11 contain a conserved sequence motif at their N-terminal regions, designated as the active phytochrome-binding (APB) motif (active phytochrome-binding protein, also named as PIL motif). Four invariant amino acid residues(ELxxxxGQ) are critical determinants of the APB motif. This motif is necessary for binding to the biologically active Pfr form of phyB [1].
Function
OsPIL11 is a member of the rice phytochrome-interacting factors (PIFs) family. Nakamura et al identified six candidate genes encoding PIFs, designated OsPIL11 to OsPIL16, via homologous analysis in rice genome. OsPIL11 contain APB motif at their N-termini, suggesting the possible interaction between PIF and phytochromes in rice .
PIFs, as a small subset of the basic helix-loop-helix (bHLH) transcription factor superfamily, have been found to bind to the G-box motif in the promoter region of light-regulated genes. Thus, PIFs constitute a signal transfer pathway from photoactivated phytochromes to the light-regulation of gene expression that controls photomorphogenesis in plants. Among phytochrome associated proteins, phytochrome-interacting factors (PIFs) are central player in phytochrome-mediated signal transduction.
In addition, OsPIL11 plays important roles in light signal transduction in rice leaves and its development, and it may also involve in the regulation of plant hormones.
Expression
OsPIF11 gene is widely expressed in rice roots, stems, new leaves and old leaves. The OsPIFs11 transcription factor expression level in the leaves is significantly higher than in the roots and stems, and the expression is the lowest in roots.
The expression of OsPIL11 also is organ-specific and is regulated by leaf development, abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). OsPIL11 is involved in red light-induced de-etiolation, but not in far-red lignt-induced de-etiolation in transgenic tobacco. OsPIL11 expressed higher in the new leaves than in the old leaves. Therefore, the expression of OsPIL11 gene was regulated by hormones in rice.
Labs working on this gene
- High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji’nan 250100, China
- College of Life Science, Henan Normal University, Xinxiang 453007, China
- College of Life Sciences, Shandong Normal University, Jinan 250014, China
- Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan
- National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
References
- ↑
Nakamura Y, Kato T, Yamashino T, Murakami M, Mizuno T. Characterization of a
set of phytochrome-interacting factor-like bHLH proteins in Oryza sativa. Biosci
Biotechnol Biochem. 2007 May;71(5):1183-91. PubMed PMID: 17485859.
Structured Information
