Difference between revisions of "Os06g0600400"
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| − | + | The rice '''''Os06g0600400''''' was reported as '''''CYP734A4''''' in 2011 <ref name="ref1" /> by researchers from Japan. | |
==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os06g0600400''''' '''''<=>''''' '''''OsCYP734A4''''','''''CYP734A4''''' | ||
| + | |||
===Function=== | ===Function=== | ||
| − | + | * Catabolism of brassinosteroids regulates the endogenous level of bioactive brassinosteroids. | |
| − | + | * The rice '''''CYP734A4''''' are multifunctional, multisubstrate enzymes that control the endogenous bioactive brassinosteroid content both by direct inactivation of CS and by the suppression of CS biosynthesis by decreasing the levels of brassinosteroid precursors. | |
===Expression=== | ===Expression=== | ||
| − | + | * Overexpression of rice '''''Os06g0600400''''' in transgenic rice gave typical brassinosteroid-deficient phenotypes. These transformants were deficient in both the bioactive CS and its precursors downstream of the C-22 hydroxylation step. | |
| − | + | * Consistent with this result, recombinant rice CYP734As utilized a range of C-22 hydroxylated brassinosteroid intermediates as substrates. | |
| − | + | * In addition, rice CYP734As can catalyze hydroxylation and the second and third oxidations to produce aldehyde and carboxylate groups at C-26 in vitro. | |
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You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8601, Japan, | |
| − | + | * Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan, | |
| − | + | * Department of Chemistry, Joetsu University of Education, Joetsu, Niigata 943-8512, Japan, | |
| − | + | * RIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan, | |
| − | + | * RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan, and | |
| − | + | * Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan | |
==References== | ==References== | ||
| − | + | <references> | |
| − | + | * <ref name="ref1"> | |
| − | + | Sakamoto T, Kawabe A, Tokida-Segawa A, Shimizu B, Takatsuto S, Shimada Y, | |
| − | + | Fujioka S, Mizutani M. Rice CYP734As function as multisubstrate and | |
| − | + | multifunctional enzymes in brassinosteroid catabolism. Plant J. 2011 | |
| − | + | Jul;67(1):1-12. doi: 10.1111/j.1365-313X.2011.04567.x. PubMed PMID: 21418356. | |
| − | + | </ref> | |
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
Latest revision as of 15:29, 7 March 2017
The rice Os06g0600400 was reported as CYP734A4 in 2011 [1] by researchers from Japan.
Contents
Annotated Information
Gene Symbol
- Os06g0600400 <=> OsCYP734A4,CYP734A4
Function
- Catabolism of brassinosteroids regulates the endogenous level of bioactive brassinosteroids.
- The rice CYP734A4 are multifunctional, multisubstrate enzymes that control the endogenous bioactive brassinosteroid content both by direct inactivation of CS and by the suppression of CS biosynthesis by decreasing the levels of brassinosteroid precursors.
Expression
- Overexpression of rice Os06g0600400 in transgenic rice gave typical brassinosteroid-deficient phenotypes. These transformants were deficient in both the bioactive CS and its precursors downstream of the C-22 hydroxylation step.
- Consistent with this result, recombinant rice CYP734As utilized a range of C-22 hydroxylated brassinosteroid intermediates as substrates.
- In addition, rice CYP734As can catalyze hydroxylation and the second and third oxidations to produce aldehyde and carboxylate groups at C-26 in vitro.
You can also add sub-section(s) at will.
Labs working on this gene
- Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8601, Japan,
- Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan,
- Department of Chemistry, Joetsu University of Education, Joetsu, Niigata 943-8512, Japan,
- RIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan,
- RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan, and
- Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
References
- ↑ Sakamoto T, Kawabe A, Tokida-Segawa A, Shimizu B, Takatsuto S, Shimada Y, Fujioka S, Mizutani M. Rice CYP734As function as multisubstrate and multifunctional enzymes in brassinosteroid catabolism. Plant J. 2011 Jul;67(1):1-12. doi: 10.1111/j.1365-313X.2011.04567.x. PubMed PMID: 21418356.
