Difference between revisions of "Os02g0181300"
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| − | + | The rice '''''Os02g0181300''''' was reported as '''''OsWRKY71''''' in 2007 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
| + | ===Gene Symbol=== | ||
| + | *'''''Os02g0181300''''' '''''<=>''''' '''''OsWRKY71''''','''''WRKY71''''' | ||
| + | |||
===Function=== | ===Function=== | ||
| − | + | * '''''OsWRKY71''''' might function in rice biotic stress response. | |
| − | ( | + | * '''''OsWRKY71''''' might function as a transcriptional regulator upstream of OsNPR1 and OsPR1b in rice defense signaling pathways. |
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * To investigate the subcellular localization of OsWRKY71 protein, the cDNA sequence of the OsWRKY71 gene was fused in frame to the 39 end of the green fluorescent protein (GFP) gene that is driven by the constitutive maize Ubiquitin (UBI) promoter. | ||
| + | * The fusion construct, UBI-GFP:OsWRKY71 and the control construct UBI-GFP were introduced, respectively, into barley aleurone cells by bombardment. Confocal microscopic observations indicated that GFP fluorescence was detected in the whole cell bombarded with the control construct UBIGFP. | ||
| + | * By contrast, the GFP:OsWRKY71 fusion protein was targeted to the nucleus. Aleurone cell nuclei were stained with SYTO17, which released red fluorescence. | ||
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * Overexpression of '''''OsWRKY71''''' gene in rice resulted in enhanced resistance to virulent bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) 13751. | ||
| + | * Furthermore, two marker genes in defense signaling pathway, OsNPR1 and OsPR1b, were constitutively expressed in '''''OsWRKY71'''''-overexpressing transgenic plants. | ||
===Expression=== | ===Expression=== | ||
| − | + | * '''''OsWRKY71''''' was up-regulated by several defense signaling molecules, such as SA, methyl jasmonate (MeJA), 1-aminocyclo-propane-1-carboxylic acid (ACC), as well as wounding and pathogen infection. | |
| + | |||
| + | ===Subcellular localization=== | ||
| + | * Transient expression of '''''OsWRKY71''''':GFP fusion protein in onion epidermis cells revealed that '''''OsWRKY71''''' was localized in the nucleus. | ||
===Evolution=== | ===Evolution=== | ||
| − | + | * WRKY proteins are a large family of transcription factors that mainly participate in plant biotic stress responses. | |
| + | * So far, one hundred and five OsWRKY genes have been predicted in the rice genome. | ||
| + | * OsWRKY IIa subfamily members play both positive and negative regulatory roles in regulating plant innate immunity. | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
==Labs working on this gene== | ==Labs working on this gene== | ||
| − | + | * National Key Laboratory of Plant Genomics and Plant Gene Research Center (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China | |
| + | * Graduate School of the Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China | ||
==References== | ==References== | ||
| − | + | <references> | |
| − | + | * <ref name="ref1"> | |
| + | Liu X, Bai X, Wang X, Chu C. OsWRKY71, a rice transcription factor, is | ||
| + | involved in rice defense response. J Plant Physiol. 2007 Aug;164(8):969-79. | ||
| + | PubMed PMID: 16919842. | ||
| + | </ref> | ||
| + | </references> | ||
==Structured Information== | ==Structured Information== | ||
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[[Category:Genes]] | [[Category:Genes]] | ||
[[Category:Japonica mRNA]] | [[Category:Japonica mRNA]] | ||
Latest revision as of 04:49, 8 March 2017
The rice Os02g0181300 was reported as OsWRKY71 in 2007 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os02g0181300 <=> OsWRKY71,WRKY71
Function
- OsWRKY71 might function in rice biotic stress response.
- OsWRKY71 might function as a transcriptional regulator upstream of OsNPR1 and OsPR1b in rice defense signaling pathways.
Phenotypic analysis
- To investigate the subcellular localization of OsWRKY71 protein, the cDNA sequence of the OsWRKY71 gene was fused in frame to the 39 end of the green fluorescent protein (GFP) gene that is driven by the constitutive maize Ubiquitin (UBI) promoter.
- The fusion construct, UBI-GFP:OsWRKY71 and the control construct UBI-GFP were introduced, respectively, into barley aleurone cells by bombardment. Confocal microscopic observations indicated that GFP fluorescence was detected in the whole cell bombarded with the control construct UBIGFP.
- By contrast, the GFP:OsWRKY71 fusion protein was targeted to the nucleus. Aleurone cell nuclei were stained with SYTO17, which released red fluorescence.
Phenotypic analysis
- Overexpression of OsWRKY71 gene in rice resulted in enhanced resistance to virulent bacterial pathogens Xanthomonas oryzae pv. oryzae (Xoo) 13751.
- Furthermore, two marker genes in defense signaling pathway, OsNPR1 and OsPR1b, were constitutively expressed in OsWRKY71-overexpressing transgenic plants.
Expression
- OsWRKY71 was up-regulated by several defense signaling molecules, such as SA, methyl jasmonate (MeJA), 1-aminocyclo-propane-1-carboxylic acid (ACC), as well as wounding and pathogen infection.
Subcellular localization
- Transient expression of OsWRKY71:GFP fusion protein in onion epidermis cells revealed that OsWRKY71 was localized in the nucleus.
Evolution
- WRKY proteins are a large family of transcription factors that mainly participate in plant biotic stress responses.
- So far, one hundred and five OsWRKY genes have been predicted in the rice genome.
- OsWRKY IIa subfamily members play both positive and negative regulatory roles in regulating plant innate immunity.
You can also add sub-section(s) at will.
Labs working on this gene
- National Key Laboratory of Plant Genomics and Plant Gene Research Center (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China
- Graduate School of the Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China
References
- ↑ Liu X, Bai X, Wang X, Chu C. OsWRKY71, a rice transcription factor, is involved in rice defense response. J Plant Physiol. 2007 Aug;164(8):969-79. PubMed PMID: 16919842.
