Difference between revisions of "Os08g0454000"

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(Created page with "'''''OsDERF1''''' (''drought-responsive ERF genes 1'') is a '''novel putative ERF protein''' in rice<ref name="ref1"/>. ==Annotated Information== ===Function=== [[File: OsDER...")
 
 
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'''''OsDERF1''''' (''drought-responsive ERF genes 1'') is a '''novel putative ERF protein''' in rice<ref name="ref1"/>.
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The rice '''''Os08g0454000''''' was reported as '''''OsERF#012''''' in 2006 <ref name="ref1" /> by researchers from Japan.  
  
 
==Annotated Information==
 
==Annotated Information==
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===Gene Symbol===
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*'''''Os08g0454000''''' '''''<=>''''' '''''OsERF#012, OsERF012, OsERF12, OsDERF1, DERF1, AP2/EREBP#070, AP2/EREBP70'''''
 
===Function===
 
===Function===
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[[File: OsDERF1 Function1.jpg|right|thumb|300px|'''Figure 1.''' ''OsDERF1 transcriptionally regulates drought response in rice.(from reference <ref name="ref1"/>).'']]
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* Genes in the ERF family encode transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants.
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*''OsDERF1'' '''negatively regulates drought stress''' through '''affecting osmolyte accumulation''' in rice. ''OsDERF1'' '''directly interacted with GCC box''' in ''OsERF3'' and ''OsAP2-39'' genes to activate the genes expression(Figure 1)<ref name="ref1"/>.
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* It has been demonstrated that the AP2/ERF proteins have important functions in the transcriptional regulation of a variety of biological processes related to growth and development, as well as various responses to environmental stimuli.
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* Genes in the AP2 family have been shown to participate in the regulation of developmental processes, e.g. flower development (Elliott et al., 1996), spikelet meristem determinacy (Chuck et al., 1998), leaf epidermal cell identity (Moose and Sisco, 1996), and embryo development (Boutilier et al., 2002).<ref name="ref2" />
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*Transcriptional activation of ''OsDERF1'' in ''OsERF3'' and ''OsAP2-39'' '''negatively regulates ethylene biosynthesis'''. '''ACC application''' partially recovers the drought tolerance of ''OsDERF1''-targeted ''OsERF3''(Figure 1). ''OsDERF1'' can not only '''activate''' the expression of '''rice ERF repressors''', but can also '''up-regulate''' the expression of ''OsGSK1'', ''OsPP2C'', ''OsABI5'' and ''OsGA2ox1''<ref name="ref1"/>.
 
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*ERF protein OsDERF1 as a '''transcriptional activator''' directly interacts with GCC box, resulting in the expression of rice repressors ''OsERF3'' and ''OsAP2-39'' (Figure 1). These repressors further '''suppress the expression''' of '''ethylene synthesis-related genes''', possibly interacting with GCC box and DRE, thereby reducing ethylene production. The decreased ethylene production disorders the hormone balance, subsequently affecting osmotic adjustment and drought response in rice<ref name="ref1"/>.
 
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===Mutation===
 
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OE and RI<ref name="ref1"/>：
 
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*Overexpression of ''OsDERF1'' (OE)
 
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*RNA interference ''OsDERF1'' rice/knockdown of ''OsDERF1''(RI)
 
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*RI '''seedling''' significantly '''decreased''' while OE lines '''enhanced malondialdehyde''' (MDA) '''accumulation under PEG treatment''', an end product of membrane lipid peroxidation, indicating that ''OsDERF1'' '''positive affects the production of oxidative stress'''.
 
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*OE seedlings had '''reduced expression''' (while RI lines showed '''enhanced expression''') of '''ethylene synthesis genes''', thereby resulting in '''changes in ethylene production'''.
 
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*OE led to '''reduced tolerance to drought''' stress in rice at '''seedling stage''', while RI expression conferred '''enhanced tolerance''' at '''seedling''' and '''tillering stages'''. This regulation was supported by negative modulation in osmotic adjustment response.
 
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*Interestingly, 979 genes showed '''two fold increases''' in OE lines compared to Nipponbare, suggesting that overexpression of ''OsDERF1'' can '''activate''' the expression of a large number of genes.
 
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===Expression===
 
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*The expression of ''OsDERF1'' is '''induced by dehydration''', '''ABA''' and '''ethylene precursor ACC'''<ref name="ref1"/>:
 
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**The expression of ''OsDERF1'' was '''highly induced by ACC''' treatment at 1 h, and reached maximum induction at 2 h, then '''decreased'''.  
 
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**The transcripts of ''OsDERF1'' in '''response to dehydration''' were '''quickly peaked''' at 0.5 h then '''decreased'''. Interestingly, the transcripts of ''OsDERF1'' showed a second increase and peaked after dehydration induction for 3 h.
 
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**The expression of ''OsDERF1'' obviously '''increased''' and '''peaked''' after '''ABA treatment''' for 1 h.
 
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**''OsDERF1'' expression '''did not significantly alter''' when seedlings were subjected to salt and cold, indicating that ''OsDERF1'' might be '''associated with ethylene'''- and '''ABA-related drought response'''.  
 
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*Overexpression of ''OsERF3''/''OsAP2-39'' '''suppressed ethylene synthesis'''. The transcripts of ''OsDERF1'' were '''highly expressed in seedling roots''' and '''sheaths''', compared to seedling leaves, whereas the expression of ''OsDERF1'' was '''mainly detected in leaves''', '''sheaths''', '''stems''', and in '''flowers''' during '''flowering stages''', indicating that the ''OsDERF1'' might '''mainly function''' at the '''tissues''' as the '''transcriptional expression'''<ref name="ref1"/>.
 
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===Subcellular localization===
 
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''OsDERF1'' is '''a nuclear-localized protein'''<ref name="ref1"/>.
 
  
 
===Evolution===
 
===Evolution===
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''OsDERF1'' located in rice '''chromosome 8''', '''encodes''' a 243-amino-acid protein with predicted molecular mass of 23.8 kDa. Amino acid analysis indicated that the putative protein '''contains a typical ERF/AP2 domain''' at 26–91th amino acids, an '''activation domain''' at 161–180th amino acids, and a '''nuclear localization signal''' at 25–34th amino acids. The '''conserved protein sequence''' implies that ''OsDERF1'' might have '''an important regulatory role''' in rice<ref name="ref1"/>.
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* The ERF family is a large gene family of transcription factors and is part of the AP2/ERF superfamily, which also contains the AP2 and RAV families
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* The AP2/ERF superfamily is defined by the AP2/ERF domain, which consists of about 60 to 70 amino acids and is involved in DNA binding.
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* The AP2 family proteins contain two repeated AP2/ERF domains, the ERF family proteins contain a single AP2/ERF domain, and the RAV family proteins contain a B3 domain, which is a DNA-binding domain conserved in other plant-specific transcription factors, including VP1/ABI3, in addition to the single AP2/ERF domain.
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* The expansion of the ERF family in plants might have been due to chromosomal/segmental duplication and tandem duplication, as well as more ancient transposition and homing.
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* Since rice is a cultivated species, selection either during domestication from its wild ancestor or during agricultural improvement in the subsequent time may also have been important for the evolution of rice ERF family.<ref name="ref3" />
  
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===Knowledge Extension===
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You can also add sub-section(s) at will.
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*Transcription factors are important among the stress-responsive genes, and their protein products are known to regulate the expression of other stress-responsive genes via binding to the regulatory elements. Among the plant transcription factors, '''ethylene response factor''' ('''ERF''') is one of the '''largest subfamilies of Apetala2 (AP2)/ERF transcription factor family''' and is characterized with single AP2 domain<ref name="ref2"/>.
 
  
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*'''ERFs''' are a double-edged sword; though most of the ERFs are activators of stress-responsive genes, certain ERF could act as repressor, and this phenomenon of ERF has been well discussed in this review. ERFs are important in plant stress management and complexity in regulation of ERF expression in response to various stresses<ref name="ref2"/>.
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==Labs working on this gene==
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* Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305–8566, Japan (T.N., K.S., H.S.);  
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* Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305–8672, Japan (T.N., T.F.)
  
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==Labs working on this gene==
 
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*Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
 
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*National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, China
 
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*National Center for Plant Gene Research (Beijing), Beijing, China
 
  
 
==References==
 
==References==
 
<references>
 
<references>
 
* <ref name="ref1">
 
* <ref name="ref1">
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Wan L, Zhang J, Zhang H, et al. Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice[J]. PLoS One, 2011, 6(9): e25216.
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Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF
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gene family in Arabidopsis and rice. Plant Physiol. 2006 Feb;140(2):411-32.
 +
PubMed PMID: 16407444; PubMed Central PMCID: PMC1361313.
 
</ref>
 
</ref>
 
* <ref name="ref2">
 
* <ref name="ref2">
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Thirugnanasambantham K, Durairaj S, Saravanan S, et al. Role of Ethylene Response Transcription Factor (ERF) and Its Regulation in Response to Stress Encountered by Plants[J]. Plant Molecular Biology Reporter, 2014: 1-11.
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Chuck G, Muszynski M, Kellogg E, Hake S, Schmidt RJ. The control of spikelet
 +
meristem identity by the branched silkless1 gene in maize. Science. 2002 Nov
 +
8;298(5596):1238-41. PubMed PMID: 12424380.
 +
</ref>
 +
* <ref name="ref3">
 +
Riechmann JL, Meyerowitz EM. The AP2/EREBP family of plant transcription
 +
factors. Biol Chem. 1998 Jun;379(6):633-46. Review. PubMed PMID: 9687012.
 
</ref>
 
</ref>
 
</references>
 
</references>
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 +
==Structured Information==
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]]

Latest revision as of 06:36, 13 March 2017

The rice Os08g0454000 was reported as OsERF#012 in 2006 [1] by researchers from Japan.

Annotated Information

Gene Symbol

  • Os08g0454000 <=> OsERF#012, OsERF012, OsERF12, OsDERF1, DERF1, AP2/EREBP#070, AP2/EREBP70

Function

  • Genes in the ERF family encode transcriptional regulators with a variety of functions involved in the developmental and physiological processes in plants.
  • It has been demonstrated that the AP2/ERF proteins have important functions in the transcriptional regulation of a variety of biological processes related to growth and development, as well as various responses to environmental stimuli.
  • Genes in the AP2 family have been shown to participate in the regulation of developmental processes, e.g. flower development (Elliott et al., 1996), spikelet meristem determinacy (Chuck et al., 1998), leaf epidermal cell identity (Moose and Sisco, 1996), and embryo development (Boutilier et al., 2002).[2]

Evolution

  • The ERF family is a large gene family of transcription factors and is part of the AP2/ERF superfamily, which also contains the AP2 and RAV families
  • The AP2/ERF superfamily is defined by the AP2/ERF domain, which consists of about 60 to 70 amino acids and is involved in DNA binding.
  • The AP2 family proteins contain two repeated AP2/ERF domains, the ERF family proteins contain a single AP2/ERF domain, and the RAV family proteins contain a B3 domain, which is a DNA-binding domain conserved in other plant-specific transcription factors, including VP1/ABI3, in addition to the single AP2/ERF domain.
  • The expansion of the ERF family in plants might have been due to chromosomal/segmental duplication and tandem duplication, as well as more ancient transposition and homing.
  • Since rice is a cultivated species, selection either during domestication from its wild ancestor or during agricultural improvement in the subsequent time may also have been important for the evolution of rice ERF family.[3]

You can also add sub-section(s) at will.

Labs working on this gene

  • Molecular and Cellular Breeding Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305–8566, Japan (T.N., K.S., H.S.);
  • Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305–8672, Japan (T.N., T.F.)


References

  1. ↑ Nakano T, Suzuki K, Fujimura T, Shinshi H. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 2006 Feb;140(2):411-32. PubMed PMID: 16407444; PubMed Central PMCID: PMC1361313.
  2. ↑ Chuck G, Muszynski M, Kellogg E, Hake S, Schmidt RJ. The control of spikelet meristem identity by the branched silkless1 gene in maize. Science. 2002 Nov 8;298(5596):1238-41. PubMed PMID: 12424380.
  3. ↑ Riechmann JL, Meyerowitz EM. The AP2/EREBP family of plant transcription factors. Biol Chem. 1998 Jun;379(6):633-46. Review. PubMed PMID: 9687012.

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os08g0454000&oldid=276904"