Difference between revisions of "Os02g0726700"

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OsbHLH133 is a regulator of iron distribution between roots and shoots in Oryza sativa.
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The rice '''Os02g0726700''' was reported as '''''OsbHLH034''''' in 2006 <ref name="ref1" /> by researchers from the China. It is a member of bHLH transcription factor gene family.
  
 
==Annotated Information==
 
==Annotated Information==
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===Gene Symbol===
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*'''''Os02g0726700''''' '''''<=>''''' '''''OsbHLH034'''''
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===Function===
 
===Function===
OsbHLH133 was found to be induced by Fe-deficiency conditions in Oryza sativa. Insertional inactivation of OsbHLH133 (bhlh133) resulted in growth retardation, with enhanced Fe concentration seen in shoots, and reduced Fe concentration in roots. Overexpression of OsbHLH133 had the opposite effect, that is resulted in an enhanced Fe concentration in roots and reduced Fe concentration in shoots and also in xylem sap. Microarray analysis showed that some of the genes encoding Fe-related functions were up-regulated under Fe-sufficient conditions, in bhlh133 mutant plants compared to wild-type plants. Significant differential expression of a number of signalling pathways, including calcium signalling, was also seen in bhlh133 plants compared to wild-type plants, independent of Fe conditions.
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* The basic/helix-loop-helix (bHLH) transcription factors and their homologs form a large family in plant and animal genomes.
 
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* rice bHLH proteins can potentially participate in a variety of combinatorial interactions, endowing them with the capacity to regulate a multitude of transcriptional programs.
1.RT-PCR analyses showed that the transcript abundance of OsbHLH133 was significantly induced by Fe deficiency in rice roots and slightly in leaves.
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* bHLHs represent key regulatory components in transcriptional networks controlling a number of biological processes.
  [[File:pce2569_f1.gif]]
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* Plant bHLHs have been reported to function in light signaling, hormone signaling, wound and drought stress responses, symbiotic ammonium transport, shoot branching, root, fruit and flower development, et al. <ref name="ref1" /> <ref name="ref2" /> <ref name="ref3" />
 
 
2.Expression of OsbHLH133 in the root of overexpression (OE) and mutant (bhlh133) plants in Fe-sufficient (+Fe) and -deficient (−Fe) conditions. As expected, no transcripts encoding OsbHLH133 could be detected in the bhlh133 mutant plants and over 100-fold induction is seen in the OE plants.
 
[[File:pce2569_f2.gif]]
 
 
 
3.Growth performance of overexpression (OE) and mutant (bhlh133) plants in Fe-sufficient (+Fe) and -deficient (−Fe) conditions. Seedlings were germinated and grown on the culture solution supplied with 100 µM (+Fe) or 2 µM EDTA-Fe (II) (−Fe) for 3 weeks. (a) Growth performance and shoot length of mutant plants under Fe-sufficient (+Fe) and -deficient (−Fe) conditions. (b) Growth performance and shoot length of OE lines under Fe-sufficient (+Fe) and -deficient (−Fe) conditions. (c) SPAD value of the youngest and fully expanded leaves. Bar = 3 cm. Data shown as the mean ± SD (n = 5). Significance (P < 0.05) of differences in (longer/shorter or higher/lower) compared to WT is indicated by an asterisk.
 
[[File:pce2569_f3.gif]]
 
  
 
===Expression===
 
===Expression===
Please input expression information here.
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* Similar expression patterns suggest functional conservation between some rice bHLH genes and their close Arabidopsis homologs.
  
 
===Evolution===
 
===Evolution===
Please input evolution information here.
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* The studies of researchers indicate that the ancient bHLH gene family has likely expanded considerably during flowering plant evolution to include many relatively young members, allowing both the conservation and divergence of gene function.
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
Please input related labs here.
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* Shanghai Jiao Tong University-Shanghai Institutes for Biological Sciences-Pennsylvania State University Joint Center for Life Sciences, Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, People’s Republic of China, 200240
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* School of Life Science, Shanghai University, Shanghai, People’s Republic of China, 200444
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* School of Life Science, Xiamen University, Xiamen, People’s Republic of China, 361005
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* Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, People’s Republic of China, 200032
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* Department of Biology and the Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802
  
 
==References==
 
==References==
Please input cited references here.
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<references>
 +
* <ref name="ref1">
 +
Li X, Duan X, Jiang H, Sun Y, Tang Y, Yuan Z, Guo J, Liang W, Chen L, Yin J,
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Ma H, Wang J, Zhang D. Genome-wide analysis of basic/helix-loop-helix
 +
transcription factor family in rice and Arabidopsis. Plant Physiol. 2006
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Aug;141(4):1167-84. PubMed PMID: 16896230; PubMed Central PMCID: PMC1533929.
 +
</ref>
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* <ref name="ref2">
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Carretero-Paulet L, Galstyan A, Roig-Villanova I, Martínez-García JF,
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Bilbao-Castro JR, Robertson DL. Genome-wide classification and evolutionary
 +
analysis of the bHLH family of transcription factors in Arabidopsis, poplar,
 +
rice, moss, and algae. Plant Physiol. 2010 Jul;153(3):1398-412. doi:
 +
10.1104/pp.110.153593. PubMed PMID: 20472752; PubMed Central PMCID: PMC2899937.
 +
</ref>
 +
* <ref name="ref3">
 +
Feller A, Machemer K, Braun EL, Grotewold E. Evolutionary and comparative
 +
analysis of MYB and bHLH plant transcription factors. Plant J. 2011
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Apr;66(1):94-116. doi: 10.1111/j.1365-313X.2010.04459.x. Review. PubMed PMID:
 +
21443626.
 +
 
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</ref>
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</references>
  
 
==Structured Information==
 
==Structured Information==
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]
 
     [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]

Latest revision as of 01:11, 22 March 2017

The rice Os02g0726700 was reported as OsbHLH034 in 2006 [1] by researchers from the China. It is a member of bHLH transcription factor gene family.

Annotated Information

Gene Symbol

  • Os02g0726700 <=> OsbHLH034

Function

  • The basic/helix-loop-helix (bHLH) transcription factors and their homologs form a large family in plant and animal genomes.
  • rice bHLH proteins can potentially participate in a variety of combinatorial interactions, endowing them with the capacity to regulate a multitude of transcriptional programs.
  • bHLHs represent key regulatory components in transcriptional networks controlling a number of biological processes.
  • Plant bHLHs have been reported to function in light signaling, hormone signaling, wound and drought stress responses, symbiotic ammonium transport, shoot branching, root, fruit and flower development, et al. [1] [2] [3]

Expression

  • Similar expression patterns suggest functional conservation between some rice bHLH genes and their close Arabidopsis homologs.

Evolution

  • The studies of researchers indicate that the ancient bHLH gene family has likely expanded considerably during flowering plant evolution to include many relatively young members, allowing both the conservation and divergence of gene function.

You can also add sub-section(s) at will.

Labs working on this gene

  • Shanghai Jiao Tong University-Shanghai Institutes for Biological Sciences-Pennsylvania State University Joint Center for Life Sciences, Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, People’s Republic of China, 200240
  • School of Life Science, Shanghai University, Shanghai, People’s Republic of China, 200444
  • School of Life Science, Xiamen University, Xiamen, People’s Republic of China, 361005
  • Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, People’s Republic of China, 200032
  • Department of Biology and the Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802

References

  1. 1.0 1.1 Li X, Duan X, Jiang H, Sun Y, Tang Y, Yuan Z, Guo J, Liang W, Chen L, Yin J, Ma H, Wang J, Zhang D. Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Arabidopsis. Plant Physiol. 2006 Aug;141(4):1167-84. PubMed PMID: 16896230; PubMed Central PMCID: PMC1533929.
  2. Carretero-Paulet L, Galstyan A, Roig-Villanova I, Martínez-García JF, Bilbao-Castro JR, Robertson DL. Genome-wide classification and evolutionary analysis of the bHLH family of transcription factors in Arabidopsis, poplar, rice, moss, and algae. Plant Physiol. 2010 Jul;153(3):1398-412. doi: 10.1104/pp.110.153593. PubMed PMID: 20472752; PubMed Central PMCID: PMC2899937.
  3. Feller A, Machemer K, Braun EL, Grotewold E. Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. Plant J. 2011 Apr;66(1):94-116. doi: 10.1111/j.1365-313X.2010.04459.x. Review. PubMed PMID: 21443626.

Structured Information