Difference between revisions of "Os09g0459200"

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Please input one-sentence summary here.
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The rice '''''Os09g0459200''''' was reported as '''''SG1''''' in 2011 by researchers from Japan.  
  
 
==Annotated Information==
 
==Annotated Information==
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[[File:12-Os09g0459200.png|right|thumb|227px|"Figure 1.(from reference<ref name="ref1"/>）."]]
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===Gene Symbol===
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*'''''Os01g0274800''''' '''''<=>''''' '''''OsSG1, Sg1'''''
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===Function===
 
===Function===
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[[File:identification.png|right|thumb|150px|"Figure 1. Identification of the rice SG1 gene by activation tagging. A to C, Phenotypes of the Sg1-Dricemutant. A,Heading stage: In
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* SG1, encodes a novel protein that is conserved among angiosperms.
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comparison with the wild-type plants (WT), the Sg1-D homozygote showed a semidwarf phenotype. B, The short-grain phenotype
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* SHORT GRAIN1 (SG1) controls the elongation of both grains and internodes in rachis branches.
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cosegregatedwith the T-DNA insertion in a dose-dependent manner. Seeds ofWT (+/+), Sg1-D heterozygotes (Sg1-D/+), and Sg1-D
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* SG1 decreases organ elongation by decreasing cell proliferation and also decreases responses to BRs and elongation of organs such as seeds and the internodes of rachis branches through decreased cellular proliferation<ref name="ref1"/>
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homozygotes (Sg1-D homo) are shown. Means and SD from at least 30 measurements of lengths (L, mm), widths (W, mm), and the
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ratio of length towidth (L/W) are shown below the seeds. C, Leaf blades of the Sg1-D homozygote were dark green and were shorter
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===Phenotypic analysis===
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and wider than those of wild type. Means and SD from at least nine measurements of lengths (L, cm) and widths (W, cm) of second
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* Overexpression of '''''SG1''''' in rice produced a phenotype with short grains and dwarfing reminiscent of brassinosteroid (BR)-deficient mutants, with wide, dark-green, and erect leaves.
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uppermost leaves are shown. D, Schematic representation of the T-DNA flanking region of the Sg1-D mutant. A candidate for the
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* However, the endogenous BR level in the SG1 overexpressor (SG1:OX) plants was comparable to the wild type. SG1:OX plants were insensitive to brassinolide in the lamina inclination assay.
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ORF responsible for the phenotype (Os09g0459200, which encodes a novel protein with unknown function, colored blue), referred
 
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to as SG1, is located 1.4-kb downstream of the T-DNA insertion. The cauliflower mosaic virus 35S minimal promoter (P35S) and a
 
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tetramer of the 35S enhancer (43EN) are shown by the black triangles and the green box, respectively. LB and RB are left and right
 
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borders of the T-DNA. Pnos, Nopaline synthase promoter sequence; PAT, ORF-containing region of the phosphinothricin
 
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acetyltransferase gene. E, The levels of SG1 mRNA in the WT, Sg1-D heterozygote, and Sg1-D homozygote plants. For each
 
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genotype, RNA from two independent plants was used for qPCR, and values were normalized using RUBQ2 as a standard. F, The
 
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Sg1-D mutant shows reduced panicle internode elongation in a dose-dependent manner. Bases of the terminal spikelets and nodes
 
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of the primary rachis branches are connectedwith dotted lines. Asterisks show that significantly different fromWT. **, P,0.01; ***,
 
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P , 0.001 (Student’s t test)(from reference<ref name="ref1"/>）." ]]
 
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SHORT GRAIN1 (SG1) controls the elongation of both grains and internodes in rachis branches.
 
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Overexpression of SG1 in rice produced a phenotype with short grains and dwarfing reminiscent
 
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of brassinosteroid (BR)-deficient mutants, with wide, dark-green, and erect leaves.SG1 decreases organ elongation by decreasing cell proliferation and also decreases responses
 
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to BRs and elongation of organs such as seeds and the internodes of rachis branches through
 
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decreased cellular proliferation<ref name="ref1"/>
 
  
 
===Expression===
 
===Expression===
  
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During vegetative growth,SG1 was preferentially expressed in the roots. During reproductive growth, SG1 was strongly expressed in the young panicles (0.3–1 cm in length), and expression then gradually decreased as the panicles matured. In the developing panicles, SG1 was expressed most strongly in the spikelets and the basal parts of rachis and rachis branches.
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* During vegetative growth,SG1 was preferentially expressed in the roots.
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SG1 encodes a novel protein that is conserved among angiosperms.SG1 does not share significant identity with any proteins of known function.
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* During reproductive growth, SG1 was strongly expressed in the young panicles (0.3–1 cm in length), and expression then gradually decreased as the panicles matured.  
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However, SG1 and related proteins comprise a family that is conserved among monocots and dicots. The rice genome encodes two SG1-related proteins (Os02g0762600 and Os08g0474100) that have 49% to 53% sequence identity with SG1.<ref name="ref1" />
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* In the developing panicles, SG1 was expressed most strongly in the spikelets and the basal parts of rachis and rachis branches.
  
 
===Evolution===
 
===Evolution===
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Please input evolution information here.
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* SG1 does not share significant identity with any proteins of known function.
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* However, SG1 and related proteins comprise a family that is conserved among monocots and dicots. The rice genome encodes two SG1-related proteins (Os02g0762600 and Os08g0474100) that have 49% to 53% sequence identity with SG1.<ref name="ref1" />
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
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==Structured Information==
 
==Structured Information==
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{{JaponicaGene|
 
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GeneName = Os09g0459200|
 
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Description = Conserved hypothetical protein|
 
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Version = NM_001069915.1 GI:115479572 GeneID:4347276|
 
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Length = 1474 bp|
 
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Definition = Oryza sativa Japonica Group Os09g0459200, complete gene.|
 
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Source = Oryza sativa Japonica Group
 
  
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  ORGANISM  Oryza sativa Japonica Group
 
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            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
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            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
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            clade; Ehrhartoideae; Oryzeae; Oryza.
 
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|
 
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Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|
 
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AP = Chromosome 9:18004651..18006124|
 
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CDS = 18004776..18005216,18005552..18005584|
 
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GCID = <gbrowseImage1>
 
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name=NC_008402:18004651..18006124
 
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source=RiceChromosome09
 
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preset=GeneLocation
 
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</gbrowseImage1>|
 
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GSID = <gbrowseImage2>
 
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name=NC_008402:18004651..18006124
 
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source=RiceChromosome09
 
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preset=GeneLocation
 
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</gbrowseImage2>|
 
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CDNA = <cdnaseq>atggcatgtgttaacatgtacaacccggagcatcaccaccaccagtcgtcgtcgttcatggcgccgaggatatccttttccagcgacttcgcgctcgagccgccgccgccggcgcagcagcagccggcggtgcgggcgcctggggacgccgacttcgagttctccgtcggcagccacccgatgatggccgccgaccagctgatctccaagggccgcctcctgccgctccgggaggccccgcacggccacggcggcgccgacgccggcggccggccactcacgctccgcgacgagctgcgcacggacagccgccatggccgcgtcccccgcgcgcccaacattcggtggaaggagttcctcggcctcaagaaggcgcccaagaaggcccccaccgccgacgctgccgccggcgccacatcgtcgtcggccgacacccagatggatcttggaggtcaagggagcacgagagactga</cdnaseq>|
 
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AA = <aaseq>MACVNMYNPEHHHHQSSSFMAPRISFSSDFALEPPPPAQQQPAV                    RAPGDADFEFSVGSHPMMAADQLISKGRLLPLREAPHGHGGADAGGRPLTLRDELRTD                    SRHGRVPRAPNIRWKEFLGLKKAPKKAPTADAAAGATSSSADTQMDLGGQGSTRD</aaseq>|
 
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DNA = <dnaseqindica>126..566#902..934#attctccatagcttggccaccgtagtaaagccggcgagctaattctagttctagccagctacacgagatcatcgctgtgacgggcggggcattgagagggcgcgtgtgccggccggggtcgatgcatggcatgtgttaacatgtacaacccggagcatcaccaccaccagtcgtcgtcgttcatggcgccgaggatatccttttccagcgacttcgcgctcgagccgccgccgccggcgcagcagcagccggcggtgcgggcgcctggggacgccgacttcgagttctccgtcggcagccacccgatgatggccgccgaccagctgatctccaagggccgcctcctgccgctccgggaggccccgcacggccacggcggcgccgacgccggcggccggccactcacgctccgcgacgagctgcgcacggacagccgccatggccgcgtcccccgcgcgcccaacattcggtggaaggagttcctcggcctcaagaaggcgcccaagaaggcccccaccgccgacgctgccgccggcgccacatcgtcgtcggccgacacccagatggtaataacaagctcactgtttcttgcgccatgaatgcactctgctacagctctgcattgtcccattttgcttgtcgcattgcaacatttttcgaatcttcaaaaacaccctgcttgctgcaagaccctcctgcgcagtagagtacaagcactttctgcttcaaagatttctcaaagtttggagcctgagatagatgtgcattgcatgcatgtactactcatgcattgctaaccaccagaaccagattgcacagaaattttagcggcacaactactcatgcattgttaaccattttcctgctgaagtgacaacaaaatgtatgcaaaaccctgtaggatcttggaggtcaagggagcacgagagactgaagcaacaaacgcaatgaagtgtattactgatcaatgagtactacgtagtagtgaaactgaagtagccatggttggccagatcggatgcatggtggcgatgcgcctttcagcttgctgtctgccatcactcatattcactcttgctgatgatcttagcagctcattctaatggagaactctgcagggcaaatggccatttccttttacctctttgctgcatcttttttgggtgtaaaagggaattttgatctctagtttttgggtaatggtcgtcgtcgggtagatgggtggttccagtgtagtcaaattaactccgctgcgcctctgatgttttgtattggaagggccagtaaaaatgtatttttccccacatggtgtggtcctagcttagcttttgtacgtgttgtttagctgttcccatgcttgtctgcttccgccacctccgctctctcccccactttccctatgtacaaaaatgcttgtaatctagtagtagctagcaattcaagctttcagttcaatgaagcccatttcaacgtt</dnaseqindica>|
 
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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069915.1 RefSeq:Os09g0459200]|
 
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}}
 
 
[[Category:Genes]]
 
[[Category:Genes]]
 
[[Category:Japonica mRNA]]
 
[[Category:Japonica mRNA]]

Latest revision as of 03:19, 7 May 2017

The rice Os09g0459200 was reported as SG1 in 2011 by researchers from Japan.

Annotated Information

"Figure 1.(from reference[1]）."

Gene Symbol

  • Os01g0274800 <=> OsSG1, Sg1

Function

  • SG1, encodes a novel protein that is conserved among angiosperms.
  • SHORT GRAIN1 (SG1) controls the elongation of both grains and internodes in rachis branches.
  • SG1 decreases organ elongation by decreasing cell proliferation and also decreases responses to BRs and elongation of organs such as seeds and the internodes of rachis branches through decreased cellular proliferation[1]

Phenotypic analysis

  • Overexpression of SG1 in rice produced a phenotype with short grains and dwarfing reminiscent of brassinosteroid (BR)-deficient mutants, with wide, dark-green, and erect leaves.
  • However, the endogenous BR level in the SG1 overexpressor (SG1:OX) plants was comparable to the wild type. SG1:OX plants were insensitive to brassinolide in the lamina inclination assay.

Expression

  • During vegetative growth,SG1 was preferentially expressed in the roots.
  • During reproductive growth, SG1 was strongly expressed in the young panicles (0.3–1 cm in length), and expression then gradually decreased as the panicles matured.
  • In the developing panicles, SG1 was expressed most strongly in the spikelets and the basal parts of rachis and rachis branches.

Evolution

  • SG1 does not share significant identity with any proteins of known function.
  • However, SG1 and related proteins comprise a family that is conserved among monocots and dicots. The rice genome encodes two SG1-related proteins (Os02g0762600 and Os08g0474100) that have 49% to 53% sequence identity with SG1.[1]

You can also add sub-section(s) at will.

Labs working on this gene

  • National Institute of Agrobiological Sciences
  • Graduate School of Science and Technology
  • Tokyo University of Science
  • RIKEN Advanced Science Institute

References

  1. ↑ 1.0 1.1 1.2 Hitoshi Nakagawa, Atsunori Tanaka, Takanari Tanabata, Miki Ohtake, Shozo Fujioka, et al. short GRAIN1 decreases organ elongation and brassinosteroid response in rice.Plant Physiol 2012 158:1208-19.

Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os09g0459200&oldid=277898"