Difference between revisions of "Os06g0493100"

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The rice '''''Os06g0493100''''' was reported as '''''OsZFP6''''' in 2014 <ref name="ref1" /> by researchers from China.  
  
 
==Annotated Information==
 
==Annotated Information==
 
===Function===
 
===Function===
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The ''Brown planthopper induced008a'' (''Bphi008a''; AY256682) gene of rice (''Oryza sativa'') enhances the plant’s resistance to the brown planthopper (BPH; ''Nilaparvata lugens''). BPH feeding rapidly initiated the ethylene (ET) signaling pathway and up-regulated ''Bphi008a'' transcript levels after 6 to 96 h of feeding. In contrast, blocking ethylene transduction (using 1-methylcyclopropene) reduced ''Bphi008a'' transcript levels in wild-type plants fed upon by BPH. Because ''Bphi008a'' is a downstream gene of the Et signaling pathway in rice. The overexpressed (OE) plants had greater resistance to the BPH than wildtype plants (Fig. 1wD). In contrast, the change in susceptibility of rice to the BPH caused by RNAi of ''Bphi008a'' was not obvious. The level of ''Bphi008a'' expression affects BPH feeding on the phloem of rice (Fig. 1wE). The ''Bphi008a'' expression enhances rice resistance to the BPH by impairing BPH feeding (Fig.1wF). The ''Bphi008a'' can be phosphorylated by rice Mitogen-activated Protein Kinase5 (OsMPK5). Yeast two-hybrid assays demonstrated that the carboxyl-terminal proline-rich region of ''Bphi008a'' interacts directly with this kinase, and this interaction occurs in the nucleus. Subsequently, we found that ''Bphi008a'' up-regulation and down-regulation were accompanied by different changes in transcription levels of OsMPK5, OsMPK12, OsMPK13, and OsMPK17 in transgenic plants (Fig. 2w). Immunoblot analysis also showed that the OsMPK5 protein level increased in overexpressing plants and decreased in RNA interference plants after BPH feeding(Fig. 3w, A and B). The ''Bphi008a'' Overexpression Enhances Levels of AP2/ERF Transcription Factors and Defense-Related Genes. Yeast two-hybrid screening results showed that a clear interaction between ''Bphi008a''and a b-ZIP transcription factor (OsbZIP60), but a weak interaction between  ''Bphi008a'' and a RNA polymerase polypeptide (SDRP) (Fig. 4wB). Furthermore, analysis of OsbZIP60 expression levels in wild-type and transgenic plants after BPH feeding from 0 to 96 h (Fig. 4wD) indicated that ''Bphi008a'' (possibly phosphorylated) might form a transcriptional complex with OsbZIP60 and SDRP in vivo that activates the transcription of target genes.<ref name="ref1" />
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* '''''OsZFP6''''' is a putatively useful gene for developing crops with increased alkali and H2O2 tolerance.
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* '''''OsZFP6''''' plays an important role in abiotic stress responses.
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<ref name="ref1" />
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===Phenotypic analysis===
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* When OsZFP6 was transformed into yeast, the transgenic yeast showed significantly increased resistance to NaHCO3 compared to the control. * Moreover, Arabidopsis transgenic plants overexpressing ''''OsZFP6''''' were more tolerant to both NaHCO3 and H2O2 treatments.
  
 
===Expression===
 
===Expression===
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In a previous study, ''Bphi008a'' expression was found to be induced by both BPH feeding and spraying plants with ethephon, which slowly releases Et.<ref name="ref2" />.In rice, a large gene family controls Et biosynthesis, composed of six 1-aminocyclopropane-1-carboxylic acid (ACC) synthase genes (designated OsACS1–OsACS6) and
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* '''''OsZFP6''''' expression was increased by abiotic stress, including salt (NaCl), alkali (NaHCO 3 ) and H 2 O 2 treatment
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seven ACC oxidase genes (designated OsACO1–OsACO7)<ref name="ref3" /><ref name="ref4" />. Experimental results indicate that Bphi008a transcription is stimulated by active Et(Fig. 5w).Bphi008a expression levels were highest in the roots of seedlings, followed (in order) by leaf blades and leaf sheaths at the heading stage, then leaf sheaths, leaf blades, and stems of seedlings. The lowest detected level of Bphi008a expression was in the flowers (Fig. 6w).
 
  
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===Evolution===
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===Subcellular localization===
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Please input evolution information here.
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* The '''''OsZFP6''''' protein contains 305 amino acids and a conserved zinc finger domain and is localised to the nucleus.
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, China.
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* Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin,Heilongjiang 150081, China
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* Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan 571737, China
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College of Life Sciences, Xinyang Normal University, China.
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* Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, Heilongjiang 150040, China College of Life Sciences, Xinyang Normal University, China.
  
 
==References==
 
==References==

Revision as of 12:31, 8 May 2017

The rice Os06g0493100 was reported as OsZFP6 in 2014 [1] by researchers from China.

Annotated Information

Function

  • OsZFP6 is a putatively useful gene for developing crops with increased alkali and H2O2 tolerance.
  • OsZFP6 plays an important role in abiotic stress responses.

[1]

Phenotypic analysis

  • When OsZFP6 was transformed into yeast, the transgenic yeast showed significantly increased resistance to NaHCO3 compared to the control. * Moreover, Arabidopsis transgenic plants overexpressing 'OsZFP6 were more tolerant to both NaHCO3 and H2O2 treatments.

Expression

  • OsZFP6 expression was increased by abiotic stress, including salt (NaCl), alkali (NaHCO 3 ) and H 2 O 2 treatment

Subcellular localization

  • The OsZFP6 protein contains 305 amino acids and a conserved zinc finger domain and is localised to the nucleus.

You can also add sub-section(s) at will.

Labs working on this gene

  • Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences, Harbin,Heilongjiang 150081, China
  • Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops, Rubber Research Institute, CATAS, Danzhou, Hainan 571737, China
  • Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, Heilongjiang 150040, China College of Life Sciences, Xinyang Normal University, China.

References

  1. ↑ 1.0 1.1 Jing Hu, Jiangbo Zhou, Xinxin Peng, Henghao Xu, Caixiang Liu, Bo Du, Hongyu Yuan, Lili Zhu, and Guangcun He. The Bphi008a Gene Interacts with the Ethylene Pathway and Transcriptionally Regulates MAPK Genes in the Response of Rice to Brown Planthopper Feeding1[C][W][OA][J].Plant Physiology, 2011, 156: 856-872.

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Structured Information

Retrieved from "https://ngdc.cncb.ac.cn/ricewiki/index.php?title=Os06g0493100&oldid=277955"