Difference between revisions of "Os09g0106700"
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| − | + | The rice '''''Os09g0106700''''' was reported as '''''PutAKT1''''' in 2009 <ref name="ref1" /> by researchers from China. | |
==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
* ''PutAKT1'' is involved in mediating K+ uptake (i) both in low- and in high-affinity K+ uptake range, and (ii)in ''P. tenuiflora'' , even under saltstress condition.The notion that ''AKT1''-type channels are the main K+ uptake pathway into the plant root was based on their dominant expression in the roots and studies of ''AKT1''-disrupted mutants. | * ''PutAKT1'' is involved in mediating K+ uptake (i) both in low- and in high-affinity K+ uptake range, and (ii)in ''P. tenuiflora'' , even under saltstress condition.The notion that ''AKT1''-type channels are the main K+ uptake pathway into the plant root was based on their dominant expression in the roots and studies of ''AKT1''-disrupted mutants. | ||
| − | *''PutAKT1'' not only increases tissue K+ accumulation, but also decreases Na+<ref name=" | + | *''PutAKT1'' not only increases tissue K+ accumulation, but also decreases Na+<ref name="ref1"/>. |
===Expression=== | ===Expression=== | ||
| Line 12: | Line 12: | ||
* Expression of ''PutAKT1'' also showed a decrease in Na+ accumulation both in the shoot and in the root. | * Expression of ''PutAKT1'' also showed a decrease in Na+ accumulation both in the shoot and in the root. | ||
====Expression in experiment==== | ====Expression in experiment==== | ||
| − | Subjected to NaCl and K+-starvation stresses for 24 h, ''PutAKT1'' was predominantly expressed in the roots under all conditions tested (about two-fold higher than in the shoots). To gain further insight into ionic stress regulation of PutAKT1 expression in roots, ''PutAKT1'' expression was monitored over a 24-h period. | + | * Subjected to NaCl and K+-starvation stresses for 24 h, ''PutAKT1'' was predominantly expressed in the roots under all conditions tested (about two-fold higher than in the shoots). To gain further insight into ionic stress regulation of PutAKT1 expression in roots, ''PutAKT1'' expression was monitored over a 24-h period. |
| − | *In rice, the expression | + | * In rice, the expression level of ''PutAKT1'' is down-regulated by excessive external NaCl. Under K+-starvation stress, ''PutAKT1'' was dramatically induced at 24 h of stress. |
| − | level of ''PutAKT1'' is down-regulated by excessive external NaCl. Under K+-starvation stress, ''PutAKT1'' was dramatically induced at 24 h of stress. | ||
===Evolution=== | ===Evolution=== | ||
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==Labs working on this gene== | ==Labs working on this gene== | ||
| − | *Asian Natural Environmental Science Center (ANESC),The University of Tokyo, 1-1-1 Midori-Cho, Nishitokyo,Tokyo 188-0002, Japan | + | * Asian Natural Environmental Science Center (ANESC),The University of Tokyo, 1-1-1 Midori-Cho, Nishitokyo,Tokyo 188-0002, Japan |
| − | *Department of Agronomy and Horticulture,Bogor Agricultural University (IPB), Jl. Meranti, Kampus IPB, Darmaga Bogor 16680, Indonesia S. Liu | + | * Department of Agronomy and Horticulture,Bogor Agricultural University (IPB), Jl. Meranti, Kampus IPB, Darmaga Bogor 16680, Indonesia S. Liu |
| − | *Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China | + | * Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China |
==References== | ==References== | ||
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Ardie SW, Liu S, Takano T. Expression of the AKT1-type K(+) channel gene from | ||
| + | Puccinellia tenuiflora, PutAKT1, enhances salt tolerance in Arabidopsis. Plant | ||
| + | Cell Rep. 2010 Aug;29(8):865-74. doi: 10.1007/s00299-010-0872-2. PubMed PMID: | ||
| + | 20532513. | ||
| + | </ref> | ||
| + | </references> | ||
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==Structured Information== | ==Structured Information== | ||
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[[Category:Genes]] | [[Category:Genes]] | ||
[[Category:Japonica mRNA]] | [[Category:Japonica mRNA]] | ||
Latest revision as of 12:54, 8 May 2017
The rice Os09g0106700 was reported as PutAKT1 in 2009 [1] by researchers from China.
Contents
Annotated Information
Function
- PutAKT1 is involved in mediating K+ uptake (i) both in low- and in high-affinity K+ uptake range, and (ii)in P. tenuiflora , even under saltstress condition.The notion that AKT1-type channels are the main K+ uptake pathway into the plant root was based on their dominant expression in the roots and studies of AKT1-disrupted mutants.
- PutAKT1 not only increases tissue K+ accumulation, but also decreases Na+[1].
Expression
- The AKT1 is preferentially expressed in peripheral root cell layers and root hairs.
- Expression of PutAKT1 increased the K+ content under normal K+ -starvation,and NaCl-stress conditions.
- The expression of PutAKT1 was induced by K+-starvation stress in the roots and was not downregulated by the presence of excess Na+.Over-expressing PutAKT1 showed enhanced salt tolerance compared to wild-type plants as shown by their shoot phenotype and dry weight.
- Expression of PutAKT1 also showed a decrease in Na+ accumulation both in the shoot and in the root.
Expression in experiment
- Subjected to NaCl and K+-starvation stresses for 24 h, PutAKT1 was predominantly expressed in the roots under all conditions tested (about two-fold higher than in the shoots). To gain further insight into ionic stress regulation of PutAKT1 expression in roots, PutAKT1 expression was monitored over a 24-h period.
- In rice, the expression level of PutAKT1 is down-regulated by excessive external NaCl. Under K+-starvation stress, PutAKT1 was dramatically induced at 24 h of stress.
Evolution
- PutAKT1 belongs to the AKT1-subfamily in the Shaker K+ channel family. PutAKT1 was localized in the plasma membrane and it was preferentially expressed in the roots.there are three AKT1-type K+ channel genes in Arabidopsis and two in rice.Genomic Southern-hybridizations using DIGlabeled PutAKT1 were performed with probes from the coding region of the PutAKT1. The result revealed a number of PutAKT1 hybridizing bands, suggesting that PutAKT1 belongs to a small gene family.
Labs working on this gene
- Asian Natural Environmental Science Center (ANESC),The University of Tokyo, 1-1-1 Midori-Cho, Nishitokyo,Tokyo 188-0002, Japan
- Department of Agronomy and Horticulture,Bogor Agricultural University (IPB), Jl. Meranti, Kampus IPB, Darmaga Bogor 16680, Indonesia S. Liu
- Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China
References
