Difference between revisions of "Os01g0757200"

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==Annotated Information==
 
==Annotated Information==
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[[File:1-f5aa.png |right |thumb |300px |''''''Figure 5. Severely Dwarfed and Semidwarfed Rice Mutants Obtained by T-DNA Activation Tagging.(A) The severe dwarf mutant GA2ox3ACT (M77777).'''''']]
 
===Function===
 
===Function===
 
The members of the rice (Oryza sativa) GA2ox family are differentially regulated and act in concert or individually to control GA levels during flowering, tillering, and seed germination.
 
The members of the rice (Oryza sativa) GA2ox family are differentially regulated and act in concert or individually to control GA levels during flowering, tillering, and seed germination.
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The three activation-tagged mutants, GA2ox5D335-341ACT,GA2ox6ACT, and GA2ox9ACT, were further characterized. Progenies displayed the same phenotypes as their parents, with GA2ox5D335-341ACT and GA2ox9ACT growing slightly shorter than the wild type, while GA2ox6ACT remained severely dwarfed throughout all growth stages (Figures 6A and 6B). GA2ox5D335-341ACT and GA2ox9ACT displayed a normal height but had longer roots and higher tiller numbers than the wild type (Table 1). Other traits significantly altered in the severe dwarf GA2ox6ACT mutant included shorter leaves, later heading date, reduced panicle length, higher tiller numbers, lower grain weight, and lower seed fertility compared with the wild type (Table 1). Germination of GA2ox6ACT seeds was also significantly delayed, as it took 20 d to reach 90% germination rate, while the wild-type and GA2ox9ACT mutant seeds took only 2 d to reach a germination rate of 97 and 98%, respectively (Figure 6C). Germination of GA2ox5D335-341ACT seeds was delayed for 4 d to reach a final 88% germination rate (Figure 6C).
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The severe dwarf mutant M77777, designated as GA2ox3ACT,carries a T-DNA insertion at a position 587 bp upstream of the translation start codon of GA2ox3 (Figure 5A). Accumulation of GA2ox3 mRNA was significantly enhanced in the heterozygous mutant. The GA2ox3ACT mutant did not produce seeds and was therefore maintained and propagated vegetatively.
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===Expression===
 
===Expression===
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Genes GA2ox1 to GA2ox9 were differentially expressed in leaves, and their expression was also temporally regulated (Figure 3B).
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Genes GA2ox3 was differentially expressed in leaves, and its expression was also temporally regulated (Figure 3B).
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However, mRNAs of GA2ox10 were not detected in any tissue at any growth stage, indicating that GA2ox10 could be a pseudogene or its mRNA level was too low to be detected Based on temporal mRNA accumulation patterns, GA2oxs could be classified into two groups. As can be seen in Figure 3B, for one group excluding GA2ox2 and GA2ox6, accumulation of their mRNAs in leaves was detected prior to the transition from vegetative to reproductive growth phases. By contrast, for another group including GA2ox2 and GA2ox6, their mRNAs accumulated in leaves after the phase transition from vegetative to reproductive growth. GA2ox6 mRNA could also be detected in leaves at early seedling stage and transiently at high level during the active tillering stage. Since expression of most GA2oxs terminated after the active tillering stage, the pattern of tiller growth throughout the rice life cycle was examined. Tiller number increased from 30 to 50 DAI (active tillering), remained constant until 75 DAI, and then increased again until 90 DAI (late tillering)when the experiment was terminated (Figure 3C). Expression of each group of GA2oxs paralleled the active and late tillering stages (cf. Figures 3B with 3C). Except for a slight reduction in the reproductive phase, the expression of GA3ox2, which encodes a GA3ox involved in GA biosynthesis, was not significantly altered in leaves throughout the rice life cycle.
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GA2ox3 accumulation of its mRNAs in leaves was detected prior to the transition from vegetative to reproductive growth phases.Since expression of most GA2oxs terminated after the active tillering stage, the pattern of tiller growth throughout the rice life cycle was examined. Tiller number increased from 30 to 50 DAI (active tillering), remained constant until 75 DAI, and then increased again until 90 DAI (late tillering)when the experiment was terminated (Figure 3C). Expression of each group of GA2oxs paralleled the active and late tillering stages (cf. Figures 3B with 3C). Germination was observed from 1 DAI and reached almost 100% at 2 DAI (Figure 4A). The accumulation of most GA2ox mRNAs was detectable starting from 0 to 1 DAI and maintained at similar levels afterward, except that of GA2ox5 and GA2ox9 was moderately reduced at 2 DAI (Figure 4B).
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===Evolution===
 
===Evolution===
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Please input evolution information here.
 
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==Labs working on this gene==
 
==Labs working on this gene==

Latest revision as of 05:56, 24 August 2017

Gibberellin 2-oxidases (GA2oxs) regulate plant growth by inactivating endogenous bioactive gibberellins (GAs).

Annotated Information

'Figure 5. Severely Dwarfed and Semidwarfed Rice Mutants Obtained by T-DNA Activation Tagging.(A) The severe dwarf mutant GA2ox3ACT (M77777).'

Function

The members of the rice (Oryza sativa) GA2ox family are differentially regulated and act in concert or individually to control GA levels during flowering, tillering, and seed germination.
The severe dwarf mutant M77777, designated as GA2ox3ACT,carries a T-DNA insertion at a position 587 bp upstream of the translation start codon of GA2ox3 (Figure 5A). Accumulation of GA2ox3 mRNA was significantly enhanced in the heterozygous mutant. The GA2ox3ACT mutant did not produce seeds and was therefore maintained and propagated vegetatively.

Expression

Genes GA2ox3 was differentially expressed in leaves, and its expression was also temporally regulated (Figure 3B). GA2ox3 accumulation of its mRNAs in leaves was detected prior to the transition from vegetative to reproductive growth phases.Since expression of most GA2oxs terminated after the active tillering stage, the pattern of tiller growth throughout the rice life cycle was examined. Tiller number increased from 30 to 50 DAI (active tillering), remained constant until 75 DAI, and then increased again until 90 DAI (late tillering)when the experiment was terminated (Figure 3C). Expression of each group of GA2oxs paralleled the active and late tillering stages (cf. Figures 3B with 3C). Germination was observed from 1 DAI and reached almost 100% at 2 DAI (Figure 4A). The accumulation of most GA2ox mRNAs was detectable starting from 0 to 1 DAI and maintained at similar levels afterward, except that of GA2ox5 and GA2ox9 was moderately reduced at 2 DAI (Figure 4B).

Evolution

Labs working on this gene

  • Institute of Molecular Biology, National Chung-Hsing University, Taichung 402, Taiwan, Republic of China
  • Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan, Republic of China
  • Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan, Republic of China
  • Department of Energy Plant Research Laboratory and Department of Plant Biology, Michigan State University,East Lansing, Michigan 48824-1312


References

Please input cited references here.

Structured Information

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