Difference between revisions of "Os02g0701300"
(Created page with "{{JaponicaGene|
GeneName = Os02g0701300|
Description = Similar to Transcription activator GRF2 (Fragment)|
Version = NM_001054378.1 GI:115448126 GeneID:4330436|
Length = 3...") |
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| − | + | The rice '''''Os02g0701300''''' was reported as '''''OsGRF4''''' in 2016 <ref name="ref1" /> by researchers from China. | |
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| − | + | ==Annotated Information== | |
| − | + | ===Gene Symbol=== | |
| − | + | *'''''Os02g0701300''''' '''''<=>''''' '''''OsGRF4''''','''''GRF4''''' | |
| − | + | ===Function=== | |
| − | + | * OsGRF4 controls grain shape, panicle length and seed shattering in rice. | |
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| − | + | ===Phenotypic analysis=== | |
| − | + | * A unique OsGRF4 mutation, which occurs at the OsmiRNA396 target site of OsGRF4, seems to be associated with high levels of OsGRF4 expression, and results in phenotypic difference. | |
| − | + | * Further research showed that OsGRF4 regulated two cytokinin dehydrogenase precursor genes (CKX5 and CKX1) resulting in increased cytokinin levels, which might affect the panicle traits. | |
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| − | + | ===Expression=== | |
| − | + | * We compared the expression profiles of OsGRF4 in various organs of NILs by quantitative RT-PCR analysis with total RNA. The OsGRF4 transcript levels varied drastically among the tissues. | |
| − | + | * OsGRF4 was preferentially expressed in developing panicles, and the highest levels of expression were found in panicles of 7 cm in length. | |
| − | + | * there was less transcript accumulation in the rice hull, root, stem and leaf sheath. In particular, the transcript was much more abundant in NIL-OsGRF4 than in NIL-Osgrf4 in the young panicles measuring 1 cm, 4 cm, 7 cm, 11 cm and 15 cm in length. | |
| − | + | * The differences corresponded with the critical stages of panicle traits. The OsGRF4 effect on panicle traits might be attributed to differences in expression levels. | |
| − | + | ||
| − | + | ===Subcellular localization=== | |
| − | < | + | * To determine the sub-cellular localization of OsGRF4, the coding sequence of OsGRF4 was fused with yellow fluorescent protein (YFP). In contrast, Ghd7, which is a nuclear protein, was fused with cyan fluorescent protein (CFP). Both fluorescent proteins were individually driven by the constitutive 35S cauliflower mosaic virus promoter. The constructs were co-transfected into rice protoplasts of etiolated seedlings by polyethylene glycol. The result showed that OsGRF4 co-localized to the nucleus with Ghd7. |
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| − | + | You can also add sub-section(s) at will. | |
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| − | + | ==Labs working on this gene== | |
| − | + | * State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China; | |
| − | [[Category:Genes]] | + | * Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland |
| − | + | * State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. | |
| − | [[Category:Oryza Sativa Japonica Group]] | + | * State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China |
| − | + | * State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. | |
| − | [[Category:Japonica | + | ==References== |
| − | + | <references> | |
| + | * <ref name="ref1"> | ||
| + | Sun P, Zhang W, Wang Y, He Q, Shu F, Liu H, Wang J, Wang J, Yuan L, Deng H. | ||
| + | OsGRF4 controls grain shape, panicle length and seed shattering in rice. J Integr | ||
| + | Plant Biol. 2016 Oct;58(10):836-847. doi: 10.1111/jipb.12473. Epub 2016 Jun 9. | ||
| + | PubMed PMID: 26936408; PubMed Central PMCID: PMC5089622. | ||
| + | </ref> | ||
| + | </references> | ||
| + | |||
| + | ==Structured Information== | ||
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]] | ||
Latest revision as of 03:57, 18 January 2018
The rice Os02g0701300 was reported as OsGRF4 in 2016 [1] by researchers from China.
Contents
Annotated Information
Gene Symbol
- Os02g0701300 <=> OsGRF4,GRF4
Function
- OsGRF4 controls grain shape, panicle length and seed shattering in rice.
Phenotypic analysis
- A unique OsGRF4 mutation, which occurs at the OsmiRNA396 target site of OsGRF4, seems to be associated with high levels of OsGRF4 expression, and results in phenotypic difference.
- Further research showed that OsGRF4 regulated two cytokinin dehydrogenase precursor genes (CKX5 and CKX1) resulting in increased cytokinin levels, which might affect the panicle traits.
Expression
- We compared the expression profiles of OsGRF4 in various organs of NILs by quantitative RT-PCR analysis with total RNA. The OsGRF4 transcript levels varied drastically among the tissues.
- OsGRF4 was preferentially expressed in developing panicles, and the highest levels of expression were found in panicles of 7 cm in length.
- there was less transcript accumulation in the rice hull, root, stem and leaf sheath. In particular, the transcript was much more abundant in NIL-OsGRF4 than in NIL-Osgrf4 in the young panicles measuring 1 cm, 4 cm, 7 cm, 11 cm and 15 cm in length.
- The differences corresponded with the critical stages of panicle traits. The OsGRF4 effect on panicle traits might be attributed to differences in expression levels.
Subcellular localization
- To determine the sub-cellular localization of OsGRF4, the coding sequence of OsGRF4 was fused with yellow fluorescent protein (YFP). In contrast, Ghd7, which is a nuclear protein, was fused with cyan fluorescent protein (CFP). Both fluorescent proteins were individually driven by the constitutive 35S cauliflower mosaic virus promoter. The constructs were co-transfected into rice protoplasts of etiolated seedlings by polyethylene glycol. The result showed that OsGRF4 co-localized to the nucleus with Ghd7.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
- Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
- State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
- State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
- State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
References
- ↑ Sun P, Zhang W, Wang Y, He Q, Shu F, Liu H, Wang J, Wang J, Yuan L, Deng H. OsGRF4 controls grain shape, panicle length and seed shattering in rice. J Integr Plant Biol. 2016 Oct;58(10):836-847. doi: 10.1111/jipb.12473. Epub 2016 Jun 9. PubMed PMID: 26936408; PubMed Central PMCID: PMC5089622.
