Difference between revisions of "Os02g0101500"

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Please input one-sentence summary here.
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The rice '''''Os02g0101500''''' was reported as '''''OsHPR1''''' in 2014 <ref name="ref1" /> by researchers from China.  
  
 
==Annotated Information==
 
==Annotated Information==
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[[File:Os02g0101500.png|right|thumb|327px|'''Figure 4.''' ''Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.<ref name="ref1" />.'']]
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===Gene Symbol===
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*'''''Os02g0101500''''' '''<=>''' '''''OsHPR1''''','''''HPR1'''''
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===Function===
 
===Function===
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Please input function information here.
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* The researchers constructed over-expression of the '''''OsHPR1''''' gene and RNA interference plants of '''''OsHPR1''''' and '''''OsHPR2''''' genes in rice.
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* The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both '''''HPR1''''' and '''''HPR2''''' were down-regulated in the same line.
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* Double deletion of '''''OsHPR1''''' and '''''OsHPR2''''' genes significantly reduces plant growth under normal photorespiration condition
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* These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.
  
 
===Expression===
 
===Expression===
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Please input expression information here.
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* Results from RT-PCR, western-blot and enzyme assays showed that '''''HPR1''''' activity changed significantly in corresponding transgenic lines without any effect on '''''HPR2''''' activity, which is the same for '''''HPR2'''''.
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===Subcellular Localization===
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* In the present study, the subcellular localization of '''''OsHPR1''''' protein was first predicted to be located in the peroxisome and cytosol respectively.
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* In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome.
  
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===Evolution===
 
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Please input evolution information here.
 
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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Please input related labs here.
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* State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
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* The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
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* State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China
  
 
==References==
 
==References==
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Please input cited references here.
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<references>
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* <ref name="ref1">
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Ye N, Yang G, Chen Y, Zhang C, Zhang J, Peng X. Two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. J Integr Plant Biol. 2014 Feb;56(2):170-80. doi: 10.1111/jipb.12125. Epub 2014 Jan 8. PubMed PMID: 24401104.
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</ref>
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</references>
  
 
==Structured Information==
 
==Structured Information==
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{{JaponicaGene|
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]
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GeneName = Os02g0101500|
 
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Description = Similar to NADH-dependent hydroxypyruvate reductase (EC 1.1.1.29) (Fragment)|
 
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Version = NM_001052124.1 GI:115443618 GeneID:4327981|
 
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Length = 4398 bp|
 
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Definition = Oryza sativa Japonica Group Os02g0101500, complete gene.|
 
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Source = Oryza sativa Japonica Group
 
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  ORGANISM  Oryza sativa Japonica Group
 
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            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
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            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
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            clade; Ehrhartoideae; Oryzeae; Oryza.
 
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|
 
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Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
 
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AP = Chromosome 2:78483..82880|
 
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CDS = 80082..80104,80194..80308,80480..80524,80726..80911,81027..81101<br>,81213..81304,81731..81803,81923..82004,82096..82217<br>,82300..82440,82509..82592,82682..82804|
 
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GCID = <gbrowseImage1>
 
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name=NC_008395:78483..82880
 
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source=RiceChromosome02
 
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preset=GeneLocation
 
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</gbrowseImage1>|
 
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GSID = <gbrowseImage2>
 
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name=NC_008395:78483..82880
 
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source=RiceChromosome02
 
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preset=GeneLocation
 
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</gbrowseImage2>|
 
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CDNA = <cdnaseq>atggcgaagccgatatcgatcgaggtgtggaatccgagcggaaagtatcgcgtggtgagcaccaagtccatgcccggcacccgctggatccgcctcctcgccgacaacgactgccgcctcgagatctgcacggagacgaagaccatcctctctgtcgacgacatcctcgccctcattggcgatcgctgcgatggtgtcatcggccagctgacggaggagtggggagaggtgctgttctcggcgctgaagcgcgccggcggaacagccttcagcaacatggccgtcgggtacaacaacgtcgacgtggaggccgccaataggaacggcatcgccgtcggcaacacgcctggcgttctgacggagacgacggcggagctggccgcgtctctgtcgctggcggcggcgcggaggatcgtggaggcagatcagttcatgagggctggcctctacgacggatggctcccgcacctgttcgtggggaacttgctcaagggtcagactgtgggagtcatcggagctggtcgaatcggctcagcttacgcaagaatgatgatcgagggcttcaagatgaacttgatctactacgacctgtatcagtccacgcgccttgagaaattcgtcacagcttacgggcagttcctgaaagcaaacggagagcagcctgtgacatggaagagagccgccaccatggaggatgtcctcagggaggccgacgtgattagtctgcaccctgtgttggacaagaccacttaccatctgataaacccggaaaggctggccatcatgaagaaggaagcggttcttgtgaatgcaagtcgaggaccggtgattgatgaggtagcactggtggagcacctcaaggccaaccccatgttccgcgtcggtcttgatgtgtttgaggatgagccttacatgaagcccggcctcgctgacatgaagaatgccgtcgtcgtacctcacatcgcatctgcctccaagtggacgcgcgaaggaatggcaacacttgctgctctaaacgttcttggcaagatcaagggttatccggtttgggggaacccgaatctagtggagccatttctcaaggaggatgcaacgccgccagctgcttgccccagcattgttaatgccaaacaactcggcctgccatcgtcaaagctctaa</cdnaseq>|
 
−
AA = <aaseq>MAKPISIEVWNPSGKYRVVSTKSMPGTRWIRLLADNDCRLEICT                    ETKTILSVDDILALIGDRCDGVIGQLTEEWGEVLFSALKRAGGTAFSNMAVGYNNVDV                    EAANRNGIAVGNTPGVLTETTAELAASLSLAAARRIVEADQFMRAGLYDGWLPHLFVG                    NLLKGQTVGVIGAGRIGSAYARMMIEGFKMNLIYYDLYQSTRLEKFVTAYGQFLKANG                    EQPVTWKRAATMEDVLREADVISLHPVLDKTTYHLINPERLAIMKKEAVLVNASRGPV                    IDEVALVEHLKANPMFRVGLDVFEDEPYMKPGLADMKNAVVVPHIASASKWTREGMAT                    LAALNVLGKIKGYPVWGNPNLVEPFLKEDATPPAACPSIVNAKQLGLPSSKL</aaseq>|
 
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DNA = <dnaseqindica>2777..2799#2573..2687#2357..2401#1970..2155#1780..1854#1577..1668#1078..1150#877..958#664..785#441..581#289..372#77..199#agtagtagagtgagctcaagacaagaagcagtagcgagtccaaggcaaggcaaggcaggcgaaagcaaaacaagaaatggcgaagccgatatcgatcgaggtgtggaatccgagcggaaagtatcgcgtggtgagcaccaagtccatgcccggcacccgctggatccgcctcctcgccgacaacgactgccgcctcgaggtaatcctttcctttccttcttaggcagcgattacttattgcattacaattacactagtagtggagagtcaattcattccttgcttcagatctgcacggagacgaagaccatcctctctgtcgacgacatcctcgccctcattggcgatcgctgcgatggtgtcatcggccaggcaagcaaagcaattgcaatccaataattttgattgatttcttcttgttgataacaaattaagtgcagctgacggaggagtggggagaggtgctgttctcggcgctgaagcgcgccggcggaacagccttcagcaacatggccgtcgggtacaacaacgtcgacgtggaggccgccaataggaacggcatcgccgtcggcaacacgcctgtcagttctgccgccctctgcttcttgatttctagactatcttactggatggtaatttacttacatacgtacacatgtacagggcgttctgacggagacgacggcggagctggccgcgtctctgtcgctggcggcggcgcggaggatcgtggaggcagatcagttcatgagggctggcctctacgacggatggctcccgcacctgtctctctctctctctctctctctttacttttactactgctgctagttgatagatactcatatatgtatatatgtatatatgtgcatgcaggttcgtggggaacttgctcaagggtcagactgtgggagtcatcggagctggtcgaatcggctcagcttacgcaagaatgatggttagttcagttggtaccttactagctaggactattattattatagtaggagtaattatttgttcaatttcgcaattgcaccacaaaattactcatgtaaacatttccttttaccacagatcgagggcttcaagatgaacttgatctactacgacctgtatcagtccacgcgccttgagaaattcgtcacaggtatgcatccaattttatagtactggctagttaatttgtactccctccgttctaaattgatctacatatttcataggtacaccaagatcaataaaaactaataactctctcatactatatttactctagcaacaaattcaatgcatgcaccatccctaatattttctagccaatagcaaatcaaaatattgcatgtgggttataaatacttgtgtgcatggatgcatgcatcaatgtccatttactccaatgcacaaataacgaatagactctaataaatgaacacaaatgtgtagatcatttagaaataatctaaaaaactatatgtagatcaatttggaatggagggagtacgacattaagatagatagattagattagattaattaatgacctgcacattgatttgaatgcatatgcatgtagcttacgggcagttcctgaaagcaaacggagagcagcctgtgacatggaagagagccgccaccatggaggatgtcctcagggaggccgacgtggtcagccatctctctcatcgtctcttctttctttcttcattctaattattaagatgttgattaattatgcaaataaatgcttgtgcatatatatatatatgtatgtggcagattagtctgcaccctgtgttggacaagaccacttaccatctgataaacccggaaaggctggccatcatgaagaaggtgcctgcctctctgcctctgttaatttcttctctcggtcctcttgatactgtagtactatgcagtgggtaattaacagagcatgtatacaaataatctaaatataaaaatacaggaagcggttcttgtgaatgcaagtcgaggaccggtgattgatgaggtagcactggtggagcacctcaaggccaaccccatgttccgcgtcggtcttgatgtgtttgaggatgagccttacatgaagcccggcctcgctgacatgaagaatgccgtcgtcgtacctcacatcgcatctgcctccaaggtccactacctgcctgctgcatctgcttatatatatatatatgcctagctagcttgttttattttctttctttctttctttcttcccataaaaatatccatccatctttacgtactactactcctacaactactagtaatatgaatcaaaaaggtactgactaatatagtatataatttgcatcattctccaattctccagtggacgcgcgaaggaatggcaacacttgctgctctaaacgttcttgtaagtactccttccatatgcatgcttaattagcctcagcccaaatattatactattgatgatgaatggaacacacactgtgtgagtgggtctgtctgtctgtgcatgtttgtatttgcactagatcgattatataaacctgctgatacggtaataataataataatgcagggcaagatcaagggttatccggtttgggggaacccgaatctagtggagccatttctcaaggaggatgcaacgccgccagctgcttgccccagcattgttaatgccaaacaactcggtcggtactactacgtaccgttagttaattcctattcattgcctagttaaaattaactttaactgcatgcgaatatatatgcgatgcaggcctgccatcgtcaaagctctaagaactggagctcggtccatcatattatatctgtcggttcaagatcatactccactacatgaagtctagatcgaggtgcatatgcttgctgttctagcggagcgggcaacaagctgtcagccagacttttgatatctaatttcacacacacacacacacacacacacacacatatatatatatatatataatatactagctagcttcaattccttgttattataccactagtacgactgtaattttgtgagtcttaaaacatgcgagctacatgtatgttattacacccgtactagctactactgtagattaattactgttacgaaatgaagataccctagctgcctgtttgggaaacgtacgattaatcatcaatgacaaagatttacagggctctagctagctagcactagcagcctgcggagagagagaggaattaattgatattagtaataactagcacatacatatgcatccttgcgtatagtaagtggagtaagtttataagggaagaaataattaagggaaaacctggcttagctgaagaagagcaagcaatactattatattatataatccaataaaagttgaaacaaaaaagagggagcaaattaaagcaaaagctagatatatataatataataatgatggacagagatatatagttagtcgaggaggagtttcttgacgtaattatcgtagacgaagtcggtgaggatgctgtaagctttctcggtggggtggtagctgtcccagaagagatagtcggagacgggggtgcagacggaggaggtgagggcgttgcagaggacggaaacctccaggaggccagtgccgcagcaacccatggtggagtcgctgaagccgtacgcccgtggacgcatcatcatgtccatcaggaagccgtagatgtccatgaaaacaagcttggtcttggtgctcttcttcttggcctgcatctcctccatcctcctcttcatcccggcgttgtacgccaccgcgatctggttgtgcccctccgagcagcgcctctccatgccccccgacatggtcctctgcgacggcacgcacccgatgggcggcatcccgatgatggccaccttcctcgccccggccttcaccagctcgtccacgaacgccgccgcgtggtgcaccagcagcgccgcgtacgaggcgtggtcgtagccgggcctcgcccgcatggtgaagtaggtgttggccacgtcgtcgctgccggcgcagatggcgaagatgcccctggtcatcatgtccgccacccgcgcgtccccggcggcgccgcgcacccgctccttgtactgctggaacagcagcagctggtcgggcatggagatgacggaggccaggcgaggcgtcagcgggtcgaagccggtgccccccgacgcgaagctgacgccggtgaggagctcctcagtcgacagctccggcgacaggtacggcgggagaagctccttcagaccaagacgggaagctgcatatgcattccgatcgatccgttgttagttgttgttgtgcatgcatgtactcctatgagaaatgagaattgacaagagaaagaaaagcatgttgctagttaccgatg</dnaseqindica>|
 
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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001052124.1 RefSeq:Os02g0101500]|
 
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}}
 
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[[Category:Genes]]
 
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[[Category:Japonica mRNA]]
 
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[[Category:Oryza Sativa Japonica Group]]
 
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[[Category:Japonica Genes]]
 
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[[Category:Japonica Chromosome 2]]
 
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[[Category:Chromosome 2]]
 

Latest revision as of 13:10, 3 October 2016

The rice Os02g0101500 was reported as OsHPR1 in 2014 [1] by researchers from China.

Annotated Information

Figure 4. Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.[1].

Gene Symbol

  • Os02g0101500 <=> OsHPR1,HPR1

Function

  • The researchers constructed over-expression of the OsHPR1 gene and RNA interference plants of OsHPR1 and OsHPR2 genes in rice.
  • The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line.
  • Double deletion of OsHPR1 and OsHPR2 genes significantly reduces plant growth under normal photorespiration condition
  • These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.

Expression

  • Results from RT-PCR, western-blot and enzyme assays showed that HPR1 activity changed significantly in corresponding transgenic lines without any effect on HPR2 activity, which is the same for HPR2.

Subcellular Localization

  • In the present study, the subcellular localization of OsHPR1 protein was first predicted to be located in the peroxisome and cytosol respectively.
  • In agreement with the prediction of HPR1 localization, the spots of HPR1-GFP and PTS1-CFP fusions were quite similar in the co-transformation protoplast, indicating the subcellular localization of HPR1 protein is in the peroxisome.


You can also add sub-section(s) at will.

Labs working on this gene

  • State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
  • The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
  • State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China

References

  1. ↑ 1.0 1.1 Ye N, Yang G, Chen Y, Zhang C, Zhang J, Peng X. Two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. J Integr Plant Biol. 2014 Feb;56(2):170-80. doi: 10.1111/jipb.12125. Epub 2014 Jan 8. PubMed PMID: 24401104.

Structured Information