Difference between revisions of "Os02g0120800"

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Please input one-sentence summary here.
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The rice '''''Os02g0120800''''' was reported as '''''OsRacB''''' in 2006 <ref name="ref1" /> by researchers from Korea.  
  
 
==Annotated Information==
 
==Annotated Information==
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===Gene Symbol===
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*'''''Os02g0120800''''' '''''<=>''''' '''''OsRacB,RacB'''''
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===Function===
 
===Function===
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Please input function information here.
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* '''''OsRacB''''' functions as a potential regulator for a basal disease resistance pathway in rice.
  
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===Phenotypic analysis===
 +
* Transgenic rice plants over-expressing '''''OsRacB''''' showed increased symptom development in response to rice blast pathogens.
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* Additionally, fluorescence microscopy of green fluorescent protein (GFP):OsRacB-transformed onion cells and Arabidopsis proto-plasts revealed OsRacB association with plasma membrane (PM), suggesting that PM localization is required for proper function of '''''OsRacB'''''.
 
===Expression===
 
===Expression===
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Please input expression information here.
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* Its mRNA was strongly expressed in leaf sheath (LS) and in panicles, but was very weakly expressed in young and mature leaves.  
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* The basal mRNA level of '''''OsRacB''''' in LS of two-week-old seedlings was strongly down-regulated upon wounding by cut and treatment with jasmonic acid.
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* A dramatic down-regulation in the '''''OsRacB''''' transcripts was also found in plants inoculated with the blast pathogen, Magna-porthe grisea.
 
===Evolution===
 
===Evolution===
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Please input evolution information here.
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* '''''OsRacB''''' is a single copy gene in the genome of rice, shared 98% identity with HvRacB at the amino acid level.
  
 
You can also add sub-section(s) at will.
 
You can also add sub-section(s) at will.
  
 
==Labs working on this gene==
 
==Labs working on this gene==
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Please input related labs here.
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* Department of Molecular Biology, College of Natural Science, Sejong University, Seoul 143-747, Korea
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* Research Laboratory for Agricultural Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal
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* Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST),
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* Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
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* School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul 136-701, Korea
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* Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women’s University, Seoul 139-774, Korea
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* Research Institute of Agricultural Resources, Ishikawa Agricultural College, Suematu, Nonoichi, Ishikawa 921-8836, Japan
  
 
==References==
 
==References==
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Please input cited references here.
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<references>
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* <ref name="ref1">
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Jung YH, Agrawal GK, Rakwal R, Kim JA, Lee MO, Choi PG, Kim YJ, Kim MJ,
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Shibato J, Kim SH, Iwahashi H, Jwa NS. Functional characterization of OsRacB
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GTPase--a potentially negative regulator of basal disease resistance in rice.
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Plant Physiol Biochem. 2006 Jan;44(1):68-77. PubMed PMID: 16531052.
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</ref>
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</references>
  
 
==Structured Information==
 
==Structured Information==
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{{JaponicaGene|
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    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]
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GeneName = Os02g0120800|
 
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Description = Similar to Small GTP-binding protein (Fragment)|
 
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Version = NM_001052247.1 GI:115443864 GeneID:4328116|
 
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Length = 3723 bp|
 
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Definition = Oryza sativa Japonica Group Os02g0120800, complete gene.|
 
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Source = Oryza sativa Japonica Group
 
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  ORGANISM  Oryza sativa Japonica Group
 
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            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 
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            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
 
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            clade; Ehrhartoideae; Oryzeae; Oryza.
 
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|
 
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Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
 
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AP = Chromosome 2:1080560..1084282|
 
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CDS = 1080923..1081024,1081111..1081176,1081306..1081370,1081938..1082001,1082398..1082507<br>,1082591..1082678,1083757..1083855|
 
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GCID = <gbrowseImage1>
 
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name=NC_008395:1080560..1084282
 
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source=RiceChromosome02
 
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preset=GeneLocation
 
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</gbrowseImage1>|
 
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GSID = <gbrowseImage2>
 
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name=NC_008395:1080560..1084282
 
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source=RiceChromosome02
 
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preset=GeneLocation
 
−
</gbrowseImage2>|
 
−
CDNA = <cdnaseq>atgagcgcgtccaggttcataaagtgcgtcaccgtcggggacggcgccgtcggcaagacctgcatgctcatctcctacacctccaacaccttccccactgattatgttccgacggtgtttgacaacttcagtgccaacgtcgtggttgatggtaacaccgtcaacctcgggctatgggacactgcaggtcaggaggattacaacagactgagaccactgagttatcgtggagctgatgttttccttctggccttctcgctaatcagcaaggccagctatgagaatgtttcaaagaagtggatacctgagctgaagcattatgcacctggtgtgcctatcatccttgtgggaacaaagcttgatcttcgagatgacaagcagttttttgtggaccatcctggtgctgttcctatcaccactgctcagggagaggaactaagaaagcaaataggcgccccatactacatcgaatgcagctcaaagacccaactaaacgtcaagggcgttttcgatgcggcaataaaggtggtgctgcagccacccaaggcgaagaagaagaaaaaggcgcaaaggggggcgtgctccattttgtga</cdnaseq>|
 
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AA = <aaseq>MSASRFIKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFS                    ANVVVDGNTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISKASYENVSKKWIP                    ELKHYAPGVPIILVGTKLDLRDDKQFFVDHPGAVPITTAQGEELRKQIGAPYYIECSS                    KTQLNVKGVFDAAIKVVLQPPKAKKKKKAQRGACSIL</aaseq>|
 
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DNA = <dnaseqindica>3259..3360#3107..3172#2913..2977#2282..2345#1776..1885#1605..1692#428..526#gccacctcctccacccacctcacccaccacctctctctctactcctcctcttctcctcctccgtctcgagcgagctctctccttccttgtctcgggtctcggcattgcatttgcattggggggcattgctattgcttcgcttgcgcttggccacccaccatttcggcgcggagaggagaccgcttcacccgccgatctgcgagaaaccctctccgcctcgcctcctccacctcccaatcttaacccaacctccccccttcctctcgcgtcgaccccttgctttgctcctccttcaaccttcttctttcttggagtttcttgagagagagagagagagagagagagagagagagagagagagaggggggagcggtcgcaggaggaggaggacggcggcgtctgctgcgaccgacggggagcggcgaggatgagcgcgtccaggttcataaagtgcgtcaccgtcggggacggcgccgtcggcaagacctgcatgctcatctcctacacctccaacaccttccccactgtgcgtattcttcttcttcttcttcttcttcttcctccaaacatttccttttcttcttcttcttctttcttttgcttcttctttttttaattatttttgaaaaaaaatacttgtgtgctcggaggcttggagctaggattatggttcttgatctggatttgggcccaaattgtggggcttttctgattcgtccgccgctcgtgaatttgggacaatgcggcttcgaattcctgcctagggttgccattcgagtttgagtaaaaaggggatcttttgcacggcaccccgtaccaaatcaaaaaccgtggctcaaatcgaagccaaatgctgcctcattcgttcctcgtgaacgctcaaacaaaaaaaatgcagtagaaaaacaaaaaattcgtccttgattgcttgcagcatttatggattgtagcagcattagcggcggaaaaaaatgcaggttttttccccccacgattcatataacgtcgaatcagtttggctggcgtttctctatcaaaggtgggaaaccgcggatccgccaggtcgagagctcgatatcgctttcccgactttctagttgtcctgtaccctcctccactcacatctcacaccttccccatggttggtaagcgcattctttgttcctttcacgcaaatccacacccaatcaattacacaccaccacccttgcttttcgcgcatggttaatttcccaattgcacattaatttccctcttttgaggagagatcttcaaaagaagagaacatttcagctgctttggggggtagattagcttattagagccctgagacatggtgaccatgtagtgggggtagtgtatccattggaccacctgtggacttcttgccatgtataataggcctggtaaaatagtaatctagtggagtatatatcagttttgtaatcttatctttaggcaagagtttgagaatggactgcacatttaccatagatatcttatcagttctgtaacagtaggattcacctcattttgtatcttatgaatcggttgtgccttgagacgatatgtgcactcattgttcgcgtcattgcttttgacaggattatgttccgacggtgtttgacaacttcagtgccaacgtcgtggttgatggtaacaccgtcaacctcgggctatgggacactgcaggtaatctggttgcgctgctatctaattctgcatgcttgttttcttctaccaagttattaagtttttccttcggaataccacaggtcaggaggattacaacagactgagaccactgagttatcgtggagctgatgttttccttctggccttctcgctaatcagcaaggccagctatgagaatgtttcaaagaaggcaagggactcactttcattttctgttaataatattgatgtgctctttttttcttctttcgctaaagaagtttgttggtcatgtttaatttgagatcgtctgtgcaatgatatgttgcttctggcatttttcttgtgtttttgccttcttcgatgataaaactattaccatgctgggaattaggctccacaaaatttagagcaggcctagatgcatccttcaatggattattccacccattagagatgatagatgatgataataatctcataaatttatgttcataggtcattgtcttatgttgatacttgatttgatcatttttgtggctcgattcacttttttagtctccacttatggtacttcttgttgattctaaactttgttatatttcagtggatacctgagctgaagcattatgcacctggtgtgcctatcatccttgtgggaacaaagcttggtaagaaccgtgcaaggctctattttgagaaatctaatcttttgtacctgtcacaagttcggttttgattttggatggaagtagtggcatgggatttttaggttgatacgattttatttggtgtgtgttcttttgatgtcaatgcttggtactggtgacgtcatttagtgataatacatgaagcagggtgctctataaaagaggaaaaatatggatatatagtaccagtatatcaatcagccatagtttttagtacttgcaagaaattcttgtgataaacctatttgaactgtttgctcttttggttctacttttgtactattcaactttggttcaacaacatgcaacagttatactctgtctcacagatttgtggcattgcacgttgtccctcagtttgctattgtcatgatggattcagttttgttgtctttttttttttggctggtgtttctacctgtcttatgaaattaaattccaatcattctacagataatatccccttcgacctgtaaagatccaactgtagcccacttacatttttcacattgtttattgcactggcagatcttcgagatgacaagcagttttttgtggaccatcctggtgctgttcctatcaccactgctcaggtacagttgtggcattgtgggttgtttaattccttatttactgtagcatcagagatgtacttgcaactcctgtggactacattgccatatcttgatcatttttttgttccttaattgccacttttgtagggagaggaactaagaaagcaaataggcgccccatactacatcgaatgcagctcaaagacccaactagtactgctttcttcccatttctttgctaaatgtgcaataacatataagatactaacagagatatcctgcccccccctgatctgcagaacgtcaagggcgttttcgatgcggcaataaaggtggtgctgcagccacccaaggcgaagaagaagaaaaaggcgcaaaggggggcgtgctccattttgtgatctaatcatcagtagacgacgaagaagaagaacgatgaagttgccaggctttattattgttgcgtcttgcttcagcgaaacagcattcatggtccggggatcctagtttactggcagctgcagcaaggcctctttgtcgaggcaatgagcgatccgtttgtttcattttctcctttctgccttgtgattatctcgtgtgactgacaagtcgtggcaattaggtaactttcctagatggtatttcctgtgtttgagaaaaaaaattcttgttatccctgtttcataagtagacatgatgtaatcgcactcagtttattcttttccttcttatttcacttcaatggaaaattatgtttccccttc</dnaseqindica>|
 
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Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001052247.1 RefSeq:Os02g0120800]|
 
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}}
 
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[[Category:Genes]]
 
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[[Category:Japonica mRNA]]
 
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[[Category:Oryza Sativa Japonica Group]]
 
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[[Category:Japonica Genes]]
 
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[[Category:Japonica Chromosome 2]]
 
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[[Category:Chromosome 2]]
 

Latest revision as of 08:42, 6 March 2017

The rice Os02g0120800 was reported as OsRacB in 2006 [1] by researchers from Korea.

Annotated Information

Gene Symbol

  • Os02g0120800 <=> OsRacB,RacB

Function

  • OsRacB functions as a potential regulator for a basal disease resistance pathway in rice.

Phenotypic analysis

  • Transgenic rice plants over-expressing OsRacB showed increased symptom development in response to rice blast pathogens.
  • Additionally, fluorescence microscopy of green fluorescent protein (GFP):OsRacB-transformed onion cells and Arabidopsis proto-plasts revealed OsRacB association with plasma membrane (PM), suggesting that PM localization is required for proper function of OsRacB.

Expression

  • Its mRNA was strongly expressed in leaf sheath (LS) and in panicles, but was very weakly expressed in young and mature leaves.
  • The basal mRNA level of OsRacB in LS of two-week-old seedlings was strongly down-regulated upon wounding by cut and treatment with jasmonic acid.
  • A dramatic down-regulation in the OsRacB transcripts was also found in plants inoculated with the blast pathogen, Magna-porthe grisea.

Evolution

  • OsRacB is a single copy gene in the genome of rice, shared 98% identity with HvRacB at the amino acid level.

You can also add sub-section(s) at will.

Labs working on this gene

  • Department of Molecular Biology, College of Natural Science, Sejong University, Seoul 143-747, Korea
  • Research Laboratory for Agricultural Biotechnology and Biochemistry (RLABB), GPO Box 8207, Kathmandu, Nepal
  • Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST),
  • Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
  • School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul 136-701, Korea
  • Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women’s University, Seoul 139-774, Korea
  • Research Institute of Agricultural Resources, Ishikawa Agricultural College, Suematu, Nonoichi, Ishikawa 921-8836, Japan

References

  1. ↑ Jung YH, Agrawal GK, Rakwal R, Kim JA, Lee MO, Choi PG, Kim YJ, Kim MJ, Shibato J, Kim SH, Iwahashi H, Jwa NS. Functional characterization of OsRacB GTPase--a potentially negative regulator of basal disease resistance in rice. Plant Physiol Biochem. 2006 Jan;44(1):68-77. PubMed PMID: 16531052.

Structured Information