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| − | Please input one-sentence summary here.
| + | The rice '''''Os07g0209000''''' was reported as '''''OsDGL1''''' in 2012 <ref name="ref1" /> by researchers from China. |
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| | ==Annotated Information== | | ==Annotated Information== |
| | + | [[File:151-Os07g0209000.png|right|thumb|327px|'''Figure 2.''' ''Increases in ROS concentration and cell death were observed in the root tips of the Osdgl1 mutant.<ref name="ref1" />.'']] |
| | + | ===Gene Symbol=== |
| | + | *'''''Os07g0209000''''' '''<=>''' '''''OsDGL1,DGL1''''' |
| | ===Function=== | | ===Function=== |
| − | Please input function information here.
| + | * The '''''OsDGL1''''' encodes the dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit precursor. |
| | + | * '''''OsDGL1''''' had a similar function in rice to that of its homolog AtDGL1 in Arabidopsis. |
| | + | * '''''OsDGL1''''' is an essential protein subunit of the oligosaccharyl-transferase (OST) complex, which is involved in N-glycosy-lation in eukaryotes. |
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| − | ===Expression=== | + | ===Phenotypic analysis=== |
| − | Please input expression information here.
| + | * The roots of the Osdgl1 mutant were more fragile than those of the WT. Staining of 7-day-old seedling roots with Evans blue (Gaff and Okong’O-gola 1971) revealed large patches of stained cells along the roots of Osdgl1 mutant plants (Fig. 2a–d), especially near the root tips, indicating that cell death had occurred. |
| | + | * Basal H2O2 concentrations were higher in the roots of the Osdgl1 mutant plants compared with WT seedlings (Fig. 2i–l). H2O2 accumulated at the root tip where cell death was observed, as evidenced by Evans blue staining (Fig. 2a–d). The degree of NBT staining, and hence the amount of O2-in the roots, was similar in WT and Osdgl1 (Fig. 2e–h). |
| | + | * The leaky rice mutant, Osdgl1, displayed a change of matrix polysaccharides in its root cell wall, shorter root cell length, smaller root meristem and cell death in the root. Consistent with the known function of the OST complex in eukaryotes, the Osdgl1 mutation leads to a defect in N-glycosylation in the root. |
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| | ===Evolution=== | | ===Evolution=== |
| − | Please input evolution information here.
| + | * '''''OsDGL1''''' is an ortholog of Arabidopsis DGL1, human OST48 and yeast WBP1. |
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
| − | Please input related labs here.
| + | * The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, PR China |
| | + | * Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, PR China |
| | | | |
| | ==References== | | ==References== |
| − | Please input cited references here.
| + | <references> |
| | + | * <ref name="ref1"> |
| | + | Qin C, Li Y, Gan J, Wang W, Zhang H, Liu Y, Wu P. OsDGL1, a homolog of an |
| | + | oligosaccharyltransferase complex subunit, is involved in N-glycosylation and |
| | + | root development in rice. Plant Cell Physiol. 2013 Jan;54(1):129-37. doi: |
| | + | 10.1093/pcp/pcs159. Epub 2012 Dec 6. PubMed PMID: 23220823. |
| | + | </ref> |
| | + | </references> |
| | | | |
| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 7]] |
| − | GeneName = Os07g0209000|
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| − | Description = Similar to Dolichyl-di-phosphooligosaccharide- protein glycotransferase (Oligosaccharyltransferase)-like|
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| − | Version = NM_001065700.1 GI:115471132 GeneID:4342702|
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| − | Length = 4461 bp|
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| − | Definition = Oryza sativa Japonica Group Os07g0209000, complete gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | | |
| − | ORGANISM Oryza sativa Japonica Group
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| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
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| − | AP = Chromosome 7:5917502..5921962|
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| − | CDS = 5917550..5917829,5918390..5918526,5918617..5918742,5919168..5919221,5919344..5919457<br>,5919895..5919950,5920017..5920058,5920580..5920655,5920765..5920836<br>,5920908..5921033,5921117..5921221,5921333..5921416,5921636..5921683<br>|
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| − | GCID = <gbrowseImage1>
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| − | name=NC_008400:5917502..5921962
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| − | source=RiceChromosome07
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| − | preset=GeneLocation
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| − | </gbrowseImage1>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_008400:5917502..5921962
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| − | source=RiceChromosome07
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | CDNA = <cdnaseq>atggcggcgccgcgccaccaccacctcgccctcgccgtcgccctcgccctcctcgtcgtcaccgccgcggcggcggacgaggggggaccgcgggggaggcgggtgctggtgctcgtggacgacctggcggtgcggtcgtcgcactcggccttcttcgcgtcgctccaggggcgagggttcgatctcgacttccgcctcgccgacgaccccaagctctcactccaccgctacggccagtacctctacgacggcctcgtcctcttcgccccctccaccccgcgttttggaggatcagtggaccaaaattcaattctggaattcatcgatgctggtcatgatatgatcctggcagcagactcttcggcctctgatcttatccgaggcattgcaacagagtgtggggttgattttgatgaggatccagaggcaatggttattgatcacattaattatgctgccactgatgctgaaggtgatcatactttgattgctggtgatgacctcatccagtcagatgtgatactggggtcaaagaaaattgaggcccctgtattatttcggggaattggacatgcggttaatccatctaacagcttggtcctgaaagtcttatccgcctctccgtcagcatactcagcaaacccaaagtctaagttggcttcccctccatctctcacagggtcggctatatcactggtttctgttatgcaggcaagaaacaatgctcgggtcttgatatctggatcactcgatctgtttagcaacaggttcctaaaatctggtgttcagaaggccggtagcaagataaggcatgagaaagctggaaatgaacaatttgtgacagaaacaagcaaatgggtcttccatgagaggggccatctaaaggcagtgaatgtcaagcataacaaggttggggagacaaatgagcctggcatgtaccgcatcaatgatgacttggaatactctgttgagatctacgaatggtctgggaccagctggaagccgtatgttgctgatgatgttcaagttcagttttacatgatgagcccttatgttctgaagacactgtcgactgacaagaagggtgtattttcaacatccttcaaagttccagatgtttatggagttttccagttcaaagttgagtaccagaggcttggatatactggtctctctctttctaagcagattccagtgcgcccttacaggcataatgagtatgaaagattcataacttcggcatatccgtactatgctgcgtcgttttcaacaatgggagccttcttcatcttctcatttgtgtacctgtaccacaaatag</cdnaseq>|
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| − | AA = <aaseq>MAAPRHHHLALAVALALLVVTAAAADEGGPRGRRVLVLVDDLAV RSSHSAFFASLQGRGFDLDFRLADDPKLSLHRYGQYLYDGLVLFAPSTPRFGGSVDQN SILEFIDAGHDMILAADSSASDLIRGIATECGVDFDEDPEAMVIDHINYAATDAEGDH TLIAGDDLIQSDVILGSKKIEAPVLFRGIGHAVNPSNSLVLKVLSASPSAYSANPKSK LASPPSLTGSAISLVSVMQARNNARVLISGSLDLFSNRFLKSGVQKAGSKIRHEKAGN EQFVTETSKWVFHERGHLKAVNVKHNKVGETNEPGMYRINDDLEYSVEIYEWSGTSWK PYVADDVQVQFYMMSPYVLKTLSTDKKGVFSTSFKVPDVYGVFQFKVEYQRLGYTGLS LSKQIPVRPYRHNEYERFITSAYPYYAASFSTMGAFFIFSFVYLYHK</aaseq>|
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| − | DNA = <dnaseqindica>49..328#889..1025#1116..1241#1667..1720#1843..1956#2394..2449#2516..2557#3079..3154#3264..3335#3407..3532#3616..3720#3832..3915#4135..4182#aaaccccacgcgaaacaaaacctccccacgccgcgagatctcgcagcaatggcggcgccgcgccaccaccacctcgccctcgccgtcgccctcgccctcctcgtcgtcaccgccgcggcggcggacgaggggggaccgcgggggaggcgggtgctggtgctcgtggacgacctggcggtgcggtcgtcgcactcggccttcttcgcgtcgctccaggggcgagggttcgatctcgacttccgcctcgccgacgaccccaagctctcactccaccgctacggccagtacctctacgacggcctcgtcctcttcgccccctccaccccgcgtaattaattttcccccacgctctctccctcattatcccccccctgctcatgagatccagtgactcagttcgtggatggatggggcttgatccgatctgtggctatgggttggtttttgcgttagatccgtagagtagagaggtcgttcgtctgatccgatctgtgttttgggagtagtggaattctgtgatctctgatgggtagctcaatccatccattgggttttggggtgttgaattctacgccaactggaaggatttaggtagtaaggttctgtagaggttatgcttagttgtttagctctagtggaaaaatgagattgtaacagtatcaaccactgcaagggctatttacttgtgttctgatttgattgctctggttgtaacagtcaagttcgtttcaattctgctgtgctacagagtaattgatttttgctcctttgtggctggttaaatctgtgggttaagttgcaaatgctttgctctctgctcgtgatgcttcagttttggtttagcctcagtcctgcgaagtcttaatgtttgtatcttttcactggcaggttttggaggatcagtggaccaaaattcaattctggaattcatcgatgctggtcatgatatgatcctggcagcagactcttcggcctctgatcttatccgaggcattgcaacagagtgtggggttgattttgatgaggtaagatatacctgtggttctaaagcttgttaatttgatcaatcgttgtctctgtttccctcagtctcatgtgtttatggatggtgccaggatccagaggcaatggttattgatcacattaattatgctgccactgatgctgaaggtgatcatactttgattgctggtgatgacctcatccagtcagatgtgatactggggtcaaagaaaattgaggttggtactaatacttcaaaaaagactcatgtctttcaaccgttctgctctttaattttttatatatttattactttcttgtaataaactgtagttggagctgagttgagactaaatcctgatcaccattgatatatttgaataatgagcatgagagtcatggactgagtactgttttggatttattcagtagcaagaatatttacagtatttgcagtcagtagaattttggctgtcatgggaacaattttggtccttcattgctgtccattgtgcccacgagtgcctgtcaaattaaattcaaaaagctttctaccacattttccactgctgtcctatgacacaatgtgtgggtaccttttctgttttttgtttacaatgtgtacttgtagtgctttcttttaataactctccctgtttactaggcccctgtattatttcggggaattggacatgcggttaatccatctaacagcttggtaagtcatttaatttctattttgttaccaaaaaattgcatgcctctattagtctattttgttactatggataggaagttttaactataatctttcatttatccttctttaaatatatgtaggtcctgaaagtcttatccgcctctccgtcagcatactcagcaaacccaaagtctaagttggcttcccctccatctctcacagggtcggctatatcactggtttctgttatgcaggtacaggatttatgcttgtctcatgtgcttgatgctatggaatttgttgctcagatttggtttagtgcttctatccttgacaaaatattaacataagacactaaaccatatttatggatcaatccatttcagttaaatggtttcctgtaaagttctgcataagtctacaacccttccttttatggccaacaaataatttagtttacattagtgttagccacctggtgtcgatgcttatgggttttgcttactttatttccttaaaagtgaaaggacaagcgaaacagttttcttggccagtaatcattccgttgttcattgttgagtattgactgctagaccaatggtctgcaaagatttgtatctcatcagttttatgtgatcaccagtatgtagctttccgatgaatttaagaagcctaatatgtccctttgcaggcaagaaacaatgctcgggtcttgatatctggatcactcgatctgtttagcaacaggtatgctaaatcgaagttgcttcctctgattttacaagattgcttattcagtgtcttttaaaataggttcctaaaatctggtgttcagaaggccggtagcaagataaggtgagcttaattacttttgaaacttttggagattagattttcaaacacttgtggtgcttataactgaatagacatacttaaaaaacatatttgagacgactgattggtcaagcctggagtaatcgtgcagtcgtgctccctctggtatagattttgcggattcttttcttgacaaatgaatggactatgtacacgtgtaggcaaacattggctgatctgattatattagaaaagagacctgtcacattattacttgaacatcaagttattaatgtttttagattgcacacagtttgcaaatatgtgcttaccgtatttgagatggttgattgctcaatatatgcagatcttagtgctgcatatttctgaatagacatacctgaaactgaaagcatattgtaacttttgtcactgtattaatgtttgtcatattatgctttgtatgttaaaaatggttgtatgatatcctcaatcaaatggaaatgatattaactttatatatttgtcatatattggtgcaggcatgagaaagctggaaatgaacaatttgtgacagaaacaagcaaatgggtcttccatgagaggggccatctaaaggttatatgttacctttatcacatttttctttgttttgaatatgtttgtttctacaacagaaattataaataagaatgatgtcataaccttgctatgtcccacttttcaggcagtgaatgtcaagcataacaaggttggggagacaaatgagcctggcatgtaccgcatcaatgatgacttggtaactctttgcctgttatatcaattgagcactgtgaagttaaacatgctaatgtaaatgattccctgaaggaatactctgttgagatctacgaatggtctgggaccagctggaagccgtatgttgctgatgatgttcaagttcagttttacatgatgagcccttatgttctgaagacactgtcgactgacaagaaggtatgagatactatgagtcagtattgcattaatgtttgtttttagcttgctgtttgttaaaataaaatttcatgccaatacagggtgtattttcaacatccttcaaagttccagatgtttatggagttttccagttcaaagttgagtaccagaggcttggatatactggtctctctctttctaagcaggttggttccacgatgctttccttttttacaacttcacacaaagcatatgcattttgcgataattatcgacctttgtaaaactgcattcaaccatttgtttctctcctgcagattccagtgcgcccttacaggcataatgagtatgaaagattcataacttcggcatatccgtactatgctgcgtcgttttcaacagtaagatatacttgatacatttcaatagacatgagtttttcttgaccttacattttagatggacatagttattgtcacttctgtagatggtagcatcttcatattctcatttgtatacctgtttccttggctggaattactaagatttttgtaaaagttacatatatgtttgtaaaagtcatgacctagctctttatataatttccctctttgttgcagatgggagccttcttcatcttctcatttgtgtacctgtaccacaaatagataactcatgagagagcacgagttacctgaggctgatgtgtgctagatccatgcactgattgtgctgtaaacccagtgttgggggttccaagttcatagaaccatatttttgtagcgcaatctagagcacctttgacgaccactatcattgataacaagaaattgtactcctgctgctagaatcttccaaccatcccccttttctttcaatcttgcctgatgaagagctgttgaaactttcatagtcaatagaatcataacaacataagcaattcggtg</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065700.1 RefSeq:Os07g0209000]|
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Chromosome 7]]
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| − | [[Category:Chromosome 7]]
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