Difference between revisions of "Os04g0434800"

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(Created page with "{{JaponicaGene| GeneName = Os04g0434800| Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)| Version = NM_001059379.2 GI:297602781 Gene...")
 
(Annotated Information)
 
(7 intermediate revisions by 3 users not shown)
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{{JaponicaGene|
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The rice '''''Os04g0434800''''' was reported as '''''OsAPX7''''' in 2014 <ref name="ref1" /> by researchers from Brazil.  
GeneName = Os04g0434800|
 
Description = Similar to Thylakoid-bound ascorbate peroxidase (EC 1.11.1.11) (Fragment)|
 
Version = NM_001059379.2 GI:297602781 GeneID:4335896|
 
Length = 4278 bp|
 
Definition = Oryza sativa Japonica Group Os04g0434800, complete gene.|
 
Source = Oryza sativa Japonica Group
 
  
  ORGANISM  Oryza sativa Japonica Group
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==Annotated Information==
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
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[[File:38-Os04g0434800.png|right|thumb|427px|'''Figure 1.''' ''Characterization of chloroplastic OsAPX7 and OsAPX8 genes in Apx7/8s knockdown plants.<ref name="ref1" />.'']]
            Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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===Gene Symbol===
            clade; Ehrhartoideae; Oryzeae; Oryza.
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*'''''Os04g0434800''''' '''''<=>''''' '''''OsAPX7''''','''''APX7''''','''''sAPX'''''
|
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Chromosome = [[:category:Japonica Chromosome 4|Chromosome 4]]|
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===Function===
AP = Chromosome 4:21645173..21649450|
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*'''''OsAPX7''''' encode chloroplastic APXs (chlAPXs).
CDS = 21645594..21645614,21645815..21645919,21646047..21646124,21646213..21646287,21646784..21646870<br>,21646967..21647034,21647354..21647441,21647560..21647660,21647746..21647866<br>,21648964..21649122,21649227..21649403|
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GCID = <gbrowseImage1>
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===Phenotypic analysis===
name=NC_008397:21645173..21649450
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* The chlAPX knockdown plants were affected more severely than the non-transformed (NT) plants in the activity and structure of PSII and CO2 assimilation in the presence of MV.
source=RiceChromosome04
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* Although MV induced significant increases in pigment content in the knockdown plants, the increases were apparently not sufficient for protection. Treatment with HL also caused generalized damage in PSII in both types of plants.
preset=GeneLocation
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* The knockdown and NT plants exhibited differences in photosynthetic parameters related to efficiency of utilization of light and CO2.  
</gbrowseImage1>|
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* The knockdown plants overexpressed other antioxidant enzymes in response to the stresses and increased the GPX activity in the chloroplast-enriched fraction.
GSID = <gbrowseImage2>
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===Expression===
name=NC_008397:21645173..21649450
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* '''''OsAPX7''''' gene was also upregulated in double mutants submitted to abiotic stress. This upregulation suggests that in spite of the knockdown of this gene, it is still able to respond to the stress in an effort to compensate for its own post-transcriptional silencing triggered by the RNAi construct.  
source=RiceChromosome04
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* However, the expression of '''''OsAPX7''''' is lower in the knockdown plants than it is in the NT plants.  
preset=GeneLocation
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</gbrowseImage2>|
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You can also add sub-section(s) at will.
CDNA = <cdnaseq>atggcggcccagcgactcgccgccctccacgccgccgcgccgtcggccttctcctccacctcctccgcctcgcatggccgccccgcggcgcgatccagcaccaccgctctcctcccggtggccctcccgcgcgcctccgccaccctccgcgccgcaccttctcggctcctcccccaggaggcgaaggcggcggggagcggcaggtcggtgatgtgcatggcgtcggcgtcggcgtcggcggcgtcggcggcggtggcgtcgggcgcggcggagctgaaggccgctcgcgaggacatcagggagctcctcaagacgacgcactgccaccccatcctggttcgtcttggatggcatgattccggtacgtatgacaagaatattaaagagtggccacaacgaggtggagctaatggaagcttgagatttgatgttgagttaaaacatggagccaatgctgggctggtaaatgctctaaagcttgtccaacctatcaaggacaaatacccaaatatctcatatgcggatttattccagctggcaagtgctacagcaattgaggaagctggtggtccaaagattccaatgacatatggacggattgatgtcacaggtcctgagcagtgtccacctgaggggaagcttcccgatgctggcccaagtgcacctgcggatcacttgaggaaggtattttacaggatgggtcttgatgacaaggagattgttgtgctgtctggagcacatacacttggaaggtccagacctgaacggagtggctgggggaaaccagaaacgaaatatactaagaatggccctggtgcacctggtgggcaatcgtggacagctgaatggcttaagtttgataacagttatttcaaggagataaaagagaaaagagatcaggatctcctggtcttgcctacagatgctgcattatttgaggacccaacattcaaggtctatgcagaaaaatacgcagaggaccaagaagcattctttaaagactatgccggagctcatgctaaactgagcaatctgggtgcaaaattcaatcctcctgagggattcacgttggacggttaa</cdnaseq>|
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AA = <aaseq>MAAQRLAALHAAAPSAFSSTSSASHGRPAARSSTTALLPVALPR                    ASATLRAAPSRLLPQEAKAAGSGRSVMCMASASASAASAAVASGAAELKAAREDIREL                    LKTTHCHPILVRLGWHDSGTYDKNIKEWPQRGGANGSLRFDVELKHGANAGLVNALKL                    VQPIKDKYPNISYADLFQLASATAIEEAGGPKIPMTYGRIDVTGPEQCPPEGKLPDAG                    PSAPADHLRKVFYRMGLDDKEIVVLSGAHTLGRSRPERSGWGKPETKYTKNGPGAPGG                    QSWTAEWLKFDNSYFKEIKEKRDQDLLVLPTDAALFEDPTFKVYAEKYAEDQEAFFKD                    YAGAHAKLSNLGAKFNPPEGFTLDG</aaseq>|
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==Labs working on this gene==
DNA = <dnaseqindica>3837..3857#3532..3636#3327..3404#3164..3238#2581..2667#2417..2484#2010..2097#1791..1891#1585..1705#329..487#48..224#aaggccacgctcccctccctcccaccaacccaccaccagcgtcacccatggcggcccagcgactcgccgccctccacgccgccgcgccgtcggccttctcctccacctcctccgcctcgcatggccgccccgcggcgcgatccagcaccaccgctctcctcccggtggccctcccgcgcgcctccgccaccctccgcgccgcaccttctcggctcctcccccaggtccgtgtcggctcggtcttgggtggtggttgggttggggtttgggacgccctggttgtttgttgttgttgactcgtgctctggctgtgtgtgtgtgatcgcaggaggcgaaggcggcggggagcggcaggtcggtgatgtgcatggcgtcggcgtcggcgtcggcggcgtcggcggcggtggcgtcgggcgcggcggagctgaaggccgctcgcgaggacatcagggagctcctcaagacgacgcactgccaccccatcctggtatgatctcgtctcgtgtacacgcaccacgcgatgcggctttggttgctccgaaacttctccttcatatcagcatcaccatgtgggagatttgattcggtagcagaggagccatctggccgatcgcctttgcaaccaaggattgcacatgagaacaaactcagctcggagacagcgatgttatgagtttgtgaacacactgaattttctctctggagacagtgatgtcatgaatctgtgaacacactgaatatgctctctggagcaatgctggaatttaacatcacgaggaagtaatttcatgaccagcttgcctctcaaataggattttgacctctagaaatagcgtattgtcgtaaatcaccaatgtgtaacgacagttcaattgttaagtctgatctcatctgtactgacgagtaggttgcacatgtcacaaatggcttgtgagagttaagagttgtattgaaagtttgaaactataaattcattcatcacgtatcctatcatcatttattgtttgtggtgataactgatactaacacttgcagcagcttttcccagtgagactatcaagtatcatgtgagttactctgtccgttctcaaatataagtatttctagccagaaatgcttacatttaggaacgtaggtcgtgcttgtgtaaattgttaggctgtgttcaattagttatcagatggtacaagtaaattctaaggaattaggcaatatgaatgcggcgttgaataattaatgacagcagaacaacattatcatagcactaggacataagtccagttcattggttatttccatgctattttaccttcatatggtcatggctatgatatgccagcttgctgaagttaaaggtgttaatagggctgccatttatccaaataatcaccattgactgaacttgtactgtcgtacattttgaatttgcaaatactgaatgcaatgcaacgggggtagaccacggcaccatttcctattgtttagttccattcaaacccttgcatgtgcacaatcaattggctttctactccagtttctctatatccacggtggttaaaatgttattcctttgcctgcatgaaggttcgtcttggatggcatgattccggtacgtatgacaagaatattaaagagtggccacaacgaggtggagctaatggaagcttgagatttgatgttgagttaaaacatggagccaatgctggtaacattaccttttcacgatttcgtttccttgttgcttgtaagtgtactttcagcaactaaaaatcctgattgaattattgaagggctggtaaatgctctaaagcttgtccaacctatcaaggacaaatacccaaatatctcatatgcggatttattccagctggcaagtgctacagcaattgaggtctgatcccctcacgcccccaaaaaataatgaaaaatgaagtacctaactagtctggttttaatgtacgaacactatattctaccttaatgatccatttttgtcaaacttgtactaggaagctggtggtccaaagattccaatgacatatggacggattgatgtcacaggtcctgagcagtgtccacctgaggggaagcttcccggttagcaacttatgtttcactggattttccaaaacactaacatctgtactgatatcctgtcttgcttacttcataattgttctttttaatttttaaatgtaaaggttcctatgtacattgtctctcatcagcttaatatatcaaccgaccttattttttcccttggaaaattatgggatagtttttattgccacgctaacaggcataaaactgttcttacatataggggtatttaggaaatattgcaccagcatcatactcaatcccttaaaactgatgcttgctaactttcagttatccttcccttttggttgctcagatgctggcccaagtgcacctgcggatcacttgaggaaggtattttacaggatgggtcttgatgacaaggtgtgcaaatgagattctagttaaccagatgttttcccatttgctttgttttatgtgatgttgagttgcatgtgccccgcgatgctgtgcctacaggagattgttgtgctgtctggagcacatacacttggaaggtccagacctgaacggagtggctgggggaaaccagaaacgaaatatactgtatgttcttcttatctttattttcttatgtgagaaccgagttttgagcatgatctcagttctatccactgtttcaatgtattcccatgttaagtctatcattgcatatcatgtcatcatttttgcaacaaggagtcaaggaccacttagtacttcatgatgttttatgctttcagtacttaatgtctctgcttaaattacccttattcctcaggaaagaaactacgtgtattaatttcaagatgtgtacatagctacagactagagtaaatcagaactccatacgttttagatatttttccttggcctctgctccgatggttttggctgaaggatgtactttttgctcacataatctctatatattctctgcaaaatatcgtgccgcctctgcaactccatgaagcaacatcaactccaagtgcatttgtctttatggttagactggattggacaaatcttgagctttttcaccttttttttcttactttttcagaagaatggccctggtgcacctggtgggcaatcgtggacagctgaatggcttaagtttgataacagttatttcaaggtacgtgccacttttcccttattcttcatgtcggatgattcatatgattttgcatgatgtgagctggtgcctggtggcaatactgcaggagataaaagagaaaagagatcaggatctcctggtcttgcctacagatgctgcattatttgaggacccaacattcaaggtgaataatgcttaaactgtactctcacttcccgaaataaaccaatctcgtactaggatatgtcaggatcatttgaaaattgaaataattattgaactaactgtctgagaattcaccgcacattcaggtctatgcagaaaaatacgcagaggaccaagaagcattctttaaagactatgccggagctcatgctaaactgagcaatctgggtgcaaaattcaatcctcctgaggtgagtgataacatcttgtccttagttttcctttccacaacacgccactcttgaccgactgggccacagttagtagagatagtttatccgacaaaccactgccaagttgtttttacccccgatgactagcgcccaatcctagtccgtggtccttctgttgtggcaaactgagagtatgtgactgtttggttgtcctgcagggattcacgttggacggttaatgcgatgcgcatgagccagatcgctgaagtggccaaccacttttacagaaaggtctatatactctactaccccatccacctcccaatttgtaggttcatgtcagctgttctacttactcgatactgtgattcaacagaaggttcgctcggttcttggctaaaatgattccaatctgctatttatcttgctcgtaggatcaagagagaaacactacacaatccaatttcctgctccaacgttgtgtcatcattccagatgaagcttttcctgaccacgagtcatattggatttggaaactgagtattcgaataaaaaaacagactggctagagaggcaaatttgagccgaacttgcggaccaaattcgacgagaaaccaaaaagaaaaacattttaatactattctttacttattatgcttt</dnaseqindica>|
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* Department of Genetics, Federal University of Rio Grande do Sul, Brazil
Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001059379.2 RefSeq:Os04g0434800]|
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* Biotechnology Center, Federal University of Rio Grande do Sul, Brazil
}}
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* Department of Botany, Federal University of Rio Grande do Sul, Brazil
[[Category:Genes]]
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* Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil
[[Category:Japonica mRNA]]
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* Department of Biophysics, Federal University of Rio Grande do Sul, Brazil
[[Category:Oryza Sativa Japonica Group]]
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[[Category:Japonica Genes]]
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==References==
[[Category:Japonica Chromosome 4]]
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<references>
[[Category:Chromosome 4]]
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* <ref name="ref1">
 +
Caverzan A, Bonifacio A, Carvalho FE, Andrade CM, Passaia G, Schünemann M,
 +
Maraschin Fdos S, Martins MO, Teixeira FK, Rauber R, Margis R, Silveira JA,
 +
Margis-Pinheiro M. The knockdown of chloroplastic ascorbate peroxidases reveals
 +
its regulatory role in the photosynthesis and protection under photo-oxidative
 +
stress in rice. Plant Sci. 2014 Jan;214:74-87. doi:
 +
10.1016/j.plantsci.2013.10.001. Epub 2013 Oct 8. PubMed PMID: 24268165.
 +
</ref>
 +
 
 +
</references>
 +
 
 +
==Structured Information==
 +
    [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 4]]

Latest revision as of 02:49, 10 October 2016

The rice Os04g0434800 was reported as OsAPX7 in 2014 [1] by researchers from Brazil.

Annotated Information

Figure 1. Characterization of chloroplastic OsAPX7 and OsAPX8 genes in Apx7/8s knockdown plants.[1].

Gene Symbol

  • Os04g0434800 <=> OsAPX7,APX7,sAPX

Function

  • OsAPX7 encode chloroplastic APXs (chlAPXs).

Phenotypic analysis

  • The chlAPX knockdown plants were affected more severely than the non-transformed (NT) plants in the activity and structure of PSII and CO2 assimilation in the presence of MV.
  • Although MV induced significant increases in pigment content in the knockdown plants, the increases were apparently not sufficient for protection. Treatment with HL also caused generalized damage in PSII in both types of plants.
  • The knockdown and NT plants exhibited differences in photosynthetic parameters related to efficiency of utilization of light and CO2.
  • The knockdown plants overexpressed other antioxidant enzymes in response to the stresses and increased the GPX activity in the chloroplast-enriched fraction.

Expression

  • OsAPX7 gene was also upregulated in double mutants submitted to abiotic stress. This upregulation suggests that in spite of the knockdown of this gene, it is still able to respond to the stress in an effort to compensate for its own post-transcriptional silencing triggered by the RNAi construct.
  • However, the expression of OsAPX7 is lower in the knockdown plants than it is in the NT plants.

You can also add sub-section(s) at will.

Labs working on this gene

  • Department of Genetics, Federal University of Rio Grande do Sul, Brazil
  • Biotechnology Center, Federal University of Rio Grande do Sul, Brazil
  • Department of Botany, Federal University of Rio Grande do Sul, Brazil
  • Department of Biochemistry and Molecular Biology, Federal University of Ceará, Brazil
  • Department of Biophysics, Federal University of Rio Grande do Sul, Brazil

References

  1. 1.0 1.1 Caverzan A, Bonifacio A, Carvalho FE, Andrade CM, Passaia G, Schünemann M, Maraschin Fdos S, Martins MO, Teixeira FK, Rauber R, Margis R, Silveira JA, Margis-Pinheiro M. The knockdown of chloroplastic ascorbate peroxidases reveals its regulatory role in the photosynthesis and protection under photo-oxidative stress in rice. Plant Sci. 2014 Jan;214:74-87. doi: 10.1016/j.plantsci.2013.10.001. Epub 2013 Oct 8. PubMed PMID: 24268165.

Structured Information