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| | ==Annotated Information== | | ==Annotated Information== |
| | ===Function=== | | ===Function=== |
| − | Hypothetical protein
| + | Please input function information here. |
| − | | |
| − | ===Mutation===
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| − | Experimental evidence for polymorphisms in orfB structural organization and mitochondrial transcript profiles of the orfB gene in the CMS-WA rice system. The sterile line orfB gene transcript profile was characterized by two transcripts of ~1.1 kb and ~0.7 kb, and one ~0.7 kb transcript was detected in the fertile lines. The ~1.1 kb transcript present in the sterile line remained unedited. However, in the presence of nuclear encoded restoration of fertility (Rf) gene(s) in fertile restored hybrid lines (APMS-6A × BR-1870; F1 generation), the ~1.1 kb orfB transcripts were fully edited. The editing of the orfB gene ~1.1 kb transcript co-segregated with fertility restoring alleles in a segregating population of F2 progeny of restored hybrid F1 plants.
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| | ===Expression=== | | ===Expression=== |
| | Please input expression information here. | | Please input expression information here. |
| − | Editing of the orfB transcripts
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| − | (a) The fertile line
| + | ===Evolution=== |
| − | Mitochondrial RNA editing of the orfB transcript was
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| − | assessed in the fertile rice line. Fourteen cDNA clones
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| − | obtained from cDNA library of fertile rice line were
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| − | sequenced. Determination of the orfB cDNA sequence
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| − | from overlapping clones from the cDNA library showed
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| − | four C®T conversions within the coding region relative
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| − | to the orfB genomic sequence.
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| − | === Evolution === | |
| − | === Knowledge Extension ===
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| | Please input evolution information here. | | Please input evolution information here. |
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| | You can also add sub-section(s) at will. | | You can also add sub-section(s) at will. |
| − | orfB transcripts of the sterile line have identical 3’ ends
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| − | with that of transcripts from the fertile lines
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| − | The basis of the observed differences in the orfB gene
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| − | transcripts between the sterile and fertile lines was
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| − | determined using 3’ RACE. The forward primer OGSP1
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| − | (Figure 4) annealed 180 bp downstream of the
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| − | initiation codon in the coding region of the orfB gene.
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| − | In both the fertile and sterile rice lines, one amplified
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| − | band of ~400 bp was obtained
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| | ==Labs working on this gene== | | ==Labs working on this gene== |
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| | ==References== | | ==References== |
| | Please input cited references here. | | Please input cited references here. |
| − | 1. Eckardt NA: Cytoplasmic male sterility and fertility restoration. Plant Cell
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| − | 2006, 18:515-517.
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| − | 2. Hanson MR: Plant mitochondrial mutation and male sterility. Annu Rev
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| − | Genet 1991, 25:461-486.
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| − | 3. Hanson MR, Bentolila S: Interactions of mitochondrial and nuclear genes
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| − | that affect male gametophyte development. Plant Cell 2004, 16:154-169.
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| − | 4. Schnable PS, Wise RP: The molecular basis of cytoplasmic male sterility
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| − | and fertility restoration. Trends Plant Sci 1998, 3:175-180.
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| − | 5. Chase CD, Babay-Laughnan S: Cytoplasmic male sterility and fertility
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| − | restoration by nuclear genes. Molecular Biology and Biotechnology of Plant
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| − | Organelles Springer-VerlagDaniell H, Chase CD 2004, 593-622.
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| − | 6. Gutierres S, Combettes B, De Paepe R: In the Nicotiana sylvestris CMSII
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| − | mutant, a recombination-mediated change 5’ to the first exon of the
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| − | mitochondrial nad1 gene is associated with lack of the NADH:
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| − | ubiquinone oxidoreductase (complex I) NADI subunit. Eur J Biochem
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| − | 1999, 216:361-370.
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| − | 7. Unseld M, Marienfeld JR, Brandt P, Brennicke A: The mitochondrial
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| − | genome of Arabidopsis thaliana contains 57 genes in 366,924
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| − | nucleotides. Nature Genet 1997, 15:57-61.
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| − | {{Mitochondrion|
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| − | GeneName = orfB|
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| − | Description = ORFB protein|
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| − | Version = GeneID:6450152|
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| − | Length = 468 bp|
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| − | Definition = Oryza sativa Japonica Group ''orfB'', Mitochondrion gene.|
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| − | Source = Oryza sativa Japonica Group
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| − | ORGANISM Oryza sativa Japonica Group
| + | ==Structured Information== |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Mitochondrion]] |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
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| − | clade; Ehrhartoideae; Oryzeae; Oryza.
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| − | |
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| − | Chromosome = [[:category:Japonica Mitochondrion|Mitochondrion]]|
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| − | AP = Mitochondrion:53424..53891|
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| − | CDS = 53424..53891|
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| − | GCID = <gbrowseImage3>
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| − | name=NC_011033:53424..53891
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| − | source=Rice_Japonica_Mitochondrion
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| − | preset=GeneLocation
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| − | </gbrowseImage3>|
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| − | GSID = <gbrowseImage2>
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| − | name=NC_011033:53424..53891
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| − | source=Rice_Japonica_Mitochondrion
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| − | preset=GeneLocation
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| − | </gbrowseImage2>|
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| − | AA = <aaseq>MPQLDKLTYFSQFFWLCLFFFSFYILLLNNNNGILGISRILKLRNQLLSHRGNKIRFKDPKNLEDILRKGFSTGLSYMYSSLSEVSQWCKTVDYLGKRRKITLISDFGEISGSRGMERQILYLISKSSYNTSSSRITCWKKIMLTHVLHGQGSII</aaseq>|
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| − | DNA = <dnaseqindica>1..468#atgcctcaacttgataaattgacttatttctcacaattcttctggttatgccttttcctctttagtttttatattctcttattaaataataataatggaatacttggaattagcagaattctcaaactacggaaccaactgctttcgcaccgggggaacaagatccgtttcaaggaccctaagaatttggaagatatctcgagaaaaggttttagcaccggtctctcatatatgtactccagtttatccgaagtatcccaatggtgtaagaccgtcgactatttgggaaaaaggaggaaaatcactctgatctcagatttcggagaaataagtggctcacgaggaatggagagacagattctctatttgatctcgaagtcctcatataacacttcttccagtcggatcacttgttggaaaaaaataatgctcacacatgttccacacgggcaaggaagcataatctaa</dnaseqindica>|
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| − | Link = [http://www.ncbi.nlm.nih.gov/gene?term=6450152 NCBI Gene:orfB]
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| − | }}
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| − | [[Category:Genes]] | |
| − | [[Category:Japonica mRNA]]
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| − | [[Category:Oryza Sativa Japonica Group]] | |
| − | [[Category:Japonica Genes]] | |
| − | [[Category:Japonica Mitochondrion]]
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| − | [[Category:Mitochondrion]]
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