Difference between revisions of "Os08g0237000"

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==Annotated Information==
 
==Annotated Information==
 
===Function===
 
===Function===
−
Xyloglucan endotransglucosylases/hydrolases (XTHs) that mediate cleavage and rejoining of the β (1-4)-xyloglucans of the primary cell wall are considered to play an important role in the construction and restructuring of xyloglucan cross-links.OsXTH8 was a single copy gene; its full-length cDNA was 1,298 bp encoding a predicted protein of 290 amino acids.OsXTH8 may play cooperative role with OsXTR1, OsXTR2, OsXTR3, OsXTR4 genes and others that are expressed in overlapping regions. OsXTH8 is involved in cell wall modification processes during rice growth and development.OsXTH8 acts to alter the structure of cell wall in response to gibberellic acid.
+
Xyloglucan endotransglucosylases/hydrolases (XTHs) that mediate cleavage and rejoining of the β (1-4)-xyloglucans of the primary cell wall are considered to play an important role in the construction and restructuring of xyloglucan cross-links.OsXTH8 was a single copy gene; its full-length cDNA was 1,298 bp encoding a predicted protein of 290 amino acids.OsXTH8 may play cooperative role with OsXTR1, OsXTR2, OsXTR3, OsXTR4 genes and others that are expressed in overlapping regions. OsXTH8 is involved in cell wall modification processes during rice growth and development.OsXTH8 acts to alter the structure of cell wall in response to gibberellic acid<ref name="ref1"/>.
 +
.
  
 
===Expression===
 
===Expression===
−
OsXTH8 was preferentially expressed in rice leaf sheath in response to gibberellic acid. OsXTH8 was highly expressed in vascular bundles of leaf sheath and young nodal roots where the cells are actively undergoing elongation and differentiation. OsXTH8 gene expression was up-regulated by gibberellic acid and there was very little effect of other hormones. Reduced accumulation of active GA was accompanied by preferential suppression of OsXTH8 expression, and OsXTH8 expression increased when exogenous GA3 was applied to the semidwarf mutant. In two genetic mutants of rice with abnormal height, the expression of OsXTH8 positively correlated with the height of the mutants. Transgenic rice expressing an RNAi construct of OsXTH8 exhibited repressed growth. These results indicate that OsXTH8 is differentially expressed in rice leaf sheath in relation to gibberellin and potentially involved in cell elongation processes.
+
OsXTH8 was preferentially expressed in rice leaf sheath in response to gibberellic acid. OsXTH8 was highly expressed in vascular bundles of leaf sheath and young nodal roots where the cells are actively undergoing elongation and differentiation. OsXTH8 gene expression was up-regulated by gibberellic acid and there was very little effect of other hormones.Reduced accumulation of active GA was accompanied by preferential suppression of OsXTH8 expression, and OsXTH8 expression increased when exogenous GA3 was applied to the semidwarf mutant. In two genetic mutants of rice with abnormal height, the expression of OsXTH8 positively correlated with the height of the mutants. Transgenic rice expressing an RNAi construct of OsXTH8 exhibited repressed growth. These results indicate that OsXTH8 is differentially expressed in rice leaf sheath in relation to gibberellin and potentially involved in cell elongation processes.
  
−
OsXTH8 was expressed in growing regions such as shoot apical meristem, vascular bundles of leaf sheath, and young crown roots developing from nodes; thus, OsXTH8 exhibited a unique expression pattern in terms of organ and stage specificity during leaf sheath elongation and young nodal root development. The expression of OsXTH8 in the shoot apex meristem, vascular bundles in leaf sheath, and youngnodal roots supports its role in cell wall modification processes during active growth.
+
OsXTH8 was expressed in growing regions such as shoot apical meristem, vascular bundles of leaf sheath, and young crown roots developing from nodes; thus, OsXTH8 exhibited a unique expression pattern in terms of organ and stage specificity during leaf sheath elongation and young nodal root development. The expression of OsXTH8 in the shoot apex meristem, vascular bundles in leaf sheath, and youngnodal roots supports its role in cell wall modification processes during active growth<ref name="ref1"/>.
  
  
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'''Alternative Organ- and Cell Type-Expression Profiles of OsXTH8'''
 
'''Alternative Organ- and Cell Type-Expression Profiles of OsXTH8'''
  
−
To examine the tissue specificity of OsXTH8, total RNAs from rice callus, root, leaf blade, and leaf sheath were hybridized with OsXTH8-specific DNA probe. A strong signal was detected in leaf sheaths but weak or no signal was observed in leaf blades, roots, and calli (Fig. 4A). These expressions were enhanced by GA3 treatment (Fig. 4A). When 1-month-old seedlings were used to characterize the expression in leaf sheath, the expression was mainly found in the three basal parts of leaf sheath (Fig. 4B). Enhanced expression of OsXTH8 in the third part of 1-month-old rice seedling, which corresponded to the second internode of leaf sheath, compared to two basal parts of leaf sheath showed that OsXTH8 is differentially expressed in leaf sheath.
+
It has been shown that different members of XTH gene family are specifically regulated by various physiological and environmental stimuli<ref name="ref2"/>.To examine the tissue specificity of OsXTH8, total RNAs from rice callus, root, leaf blade, and leaf sheath were hybridized with OsXTH8-specific DNA probe. A strong signal was detected in leaf sheaths but weak or no signal was observed in leaf blades, roots, and calli (Fig. 4A). These expressions were enhanced by GA3 treatment (Fig. 4A). When 1-month-old seedlings were used to characterize the expression in leaf sheath, the expression was mainly found in the three basal parts of leaf sheath (Fig. 4B). Enhanced expression of OsXTH8 in the third part of 1-month-old rice seedling, which corresponded to the second internode of leaf sheath, compared to two basal parts of leaf sheath showed that OsXTH8 is differentially expressed in leaf sheath<ref name="ref1"/>.
  
−
In situ hybridization was performed using the basal part (culm) of 2-week-old rice seedlings to learn more about the expression pattern. On hybridization with gene-specific OsXTH8 antisense probe, the cross and longitudinal sections of culm tissue revealed an accumulation of OsXTH8 mRNA in shoot apex meristem, vascular tissues, and young leaves (Fig. 5A). Although the expression did not seem to be delimited to specific cell types, significant hybridization was observed in large and small vascular bundles of leaf sheath and peripheral cylinder of the vascular bundles and fibers in the nodal region (Fig. 5A). No significant signal was visible when sense probe was used.
+
[[File:dami3.jpg|caption]]
−
 
+
[[File:dami4.jpg|caption]]  
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To further characterize the spatial distribution patterns of OsXTH8 gene expression, 2,325 bp of the OsXTH8 upstream region from the proposed first translational start site was fused to the β-glucuronidase (GUS) reporter gene. This fusion gene was introduced into rice cells, and transgenic plants were regenerated. Putative OsXTH8::GUS transgenic plants were screened by PCR. Only PCR-confirmed, transgenic lines were used for GUS staining and GUS assay. To assess whether the GUS staining patterns were consistent with the result of in situ hybridization, similartissue sections of leaf sheath were used. Figure 5Bshows the GUS expression pattern driven by OsXTH8 promoter in the basal part of leaf sheath and young nodal roots of 2-week-old rice seedlings. In the case of leaf sheath, strong GUS staining was observed in shoot apex meristem and vascular bundles, very much similar to the result of in situ hybridization (Fig. 5, A and B). Microscopic observation of vascular bundles revealed GUS expression in vascular bundle sheath and mesotomic sheath surrounding xylem and phloem. Stele or vascular cylinder region in young nodal roots of the coleoptile node and roots arising from nodal roots also showed GUS staining (Fig. 5B). Weak GUS staining was found in the sclerenchyma cells lining the epidermis of young leaves (Fig. 5B).
+
[[File:dami5.jpg|caption]]
−
 
 
−
To examine the tissue specificity of OsXTH8, total RNAs from rice callus, root, leaf blade, and leaf sheath were hybridized with OsXTH8-specific DNA probe. A strong signal was detected in leaf sheaths but weak or no signal was observed in leaf blades, roots, and calli (Fig. 4A). These expressions were enhanced by GA3 treatment (Fig. 4A). When 1-month-old seedlings were used to characterize the expression in leaf sheath, the expression was mainly found in the three basal parts of leaf sheath (Fig. 4B). Enhanced expression of OsXTH8 in the third part of 1-month-old rice seedling, which corresponded to the second internode of leaf sheath, compared to two basal parts of leaf sheath showed that OsXTH8 is differentially expressed in leaf sheath.
 
−
 
 
−
In situ hybridization was performed using the basal part (culm) of 2-week-old rice seedlings to learn more about the expression pattern. On hybridization with gene-specific OsXTH8 antisense probe, the cross and longitudinal sections of culm tissue revealed an accumulation of OsXTH8 mRNA in shoot apex meristem, vascular tissues, and young leaves (Fig. 5A). Although the expression did not seem to be delimited to specific cell types, significant hybridization was observed in large and small vascular bundles of leaf sheath and peripheral cylinder of the vascular bundles and fibers in the nodal region (Fig. 5A). No significant signal was visible when sense probe was used.
 
−
 
 
−
To further characterize the spatial distribution patterns of OsXTH8 gene expression, 2,325 bp of the OsXTH8 upstream region from the proposed first translational start site was fused to the β-glucuronidase (GUS) reporter gene. This fusion gene was introduced into rice cells, and transgenic plants were regenerated. Putative OsXTH8::GUS transgenic plants were screened by PCR. Only PCR-confirmed, transgenic lines were used for GUS staining and GUS assay. To assess whether the GUS staining patterns were consistent with the result of in situ hybridization, similartissue sections of leaf sheath were used. Figure 5Bshows the GUS expression pattern driven by OsXTH8 promoter in the basal part of leaf sheath and young nodal roots of 2-week-old rice seedlings. In the case of leaf sheath, strong GUS staining was observed in shoot apex meristem and vascular bundles, very much similar to the result of in situ hybridization (Fig. 5, A and B). Microscopic observation of vascular bundles revealed GUS expression in vascular bundle sheath and mesotomic sheath surrounding xylem and phloem. Stele or vascular cylinder region in young nodal roots of the coleoptile node and roots arising from nodal roots also showed GUS staining (Fig. 5B). Weak GUS staining was found in the sclerenchyma cells lining the epidermis of young leaves (Fig. 5B).
 
−
 
 
−
[[File:dami3.jpg|caption]]
 
−
 
 
−
'''Hormonal Regulation of OsXTH8 Expression'''
 
−
 
 
−
GA dose-dependent expression patterns of ''OsXTH8'' mRNA were determined in rice leaf sheaths using different concentration of GA3 (1, 5, 10, and 50 μM). ''OsXTH8'' expression was found to be up-regulated with the increase in GA3 concentration as there was no inhibitory effect of increase in GA3 concentration up to 50 μM; however, 5 μM GA3 induced maximum expression of ''OsXTH8'' (Fig. 6A). To determine temporal expression patterns of ''OsXTH8'' mRNA, leaf sheaths were treated for 1, 3, 6, 12, and 24 h. ''OsXTH8'' mRNA accumulation in leaf sheath showed that during treatment of leaf sheath fragments with GA3, ''OsXTH8'' expression was up-regulated and continued to increase throughout the 24-h incubation period (Fig. 6B). As 2-week-old rice seedlings were used for timecourse experiment, because of the growth of leaf sheaths, increase in ''OsXTH8'' expression in control could also be detected.
 
−
 
 
−
It has been shown that ''XTH'' genes are regulated by various hormones. For example, BRU1, a soybean ''XTH'', is regulated by BR (Zurek and Clouse, 1994), and TCH4, an Arabidopsis (''Arabidopsis thaliana'') XTH gene, is up-regulated by auxin and BR (Xu et al., 1995). To characterize hormonal regulation of ''OsXTH8'' expression, the effect of several plant hormones was examined on ''OsXTH8'' mRNA abundance (Fig. 6C). When leaf sheaths were treated with GA3, brassinolide (BL), 6-bezyladenine (BA), indole-3-acetic acid (IAA), and abscisic acid (ABA), GA3 up-regulated the expression of OsXTH8 and there was very little effect of other hormones. Uniconazole, which is a potent GA biosynthesis inhibitor, had an inhibitory effect on the OsXTH8 mRNA accumulation (Fig. 6C).
 
−
 
 
−
To understand the physiological functions of ''OsXTH8'', its expression in rice mutants with abnormal heights was investigated. Tanginbozu is a GA-deficient semidwarf mutant and a single recessive gene controls the semidwarfism of Tanginbozu. Mutation in Tanginbozu blocks the three oxidative steps whereby ent-kaurene is converted to ent-kaurenoic acid resulting in less accumulation of active GA (Ogawa et al., 1996). Northern-blot analysis showed that the level of ''OsXTH8'' mRNA in the mutant was lower than that in its wild-type cv Ginbozu (Fig. 6D). The expression of ''OsXTH8'' in the mutant was induced to exceed wildtype level following treatment with GA3 for 24 h. ''Slender rice1 (slr1)'', a GA-insensitive mutant, shows a constitutive GA-response phenotype (Itoh et al., 2002). Stem of the ''slr1'' mutant grows 2 to 3 times more than the stem of wild-type cv Nipponbare. Northernblot analysis confirmed that the level of ''OsXTH8'' expression was higher in slr1 mutant than that of its wild type (Fig. 6D).
 
−
 
 
−
To analyze whether the 2,325-bp 5’-promoter regionof the ''OsXTH8'' locus is sufficient for the induction ofits expression, an independent transgenic line (Fig.7B), which was transformed with 2,325 bp of ''OsXTH8'' promoter fused to the GUS reporter gene, was treated with 5 μM GA3. It was observed that GA3 treatment enhanced the expression of the GUS reporter gene compared to untreated (mock) OsXTH8::GUS transgenic rice (Fig. 7B). This indicated that the 2,325-bp promoter region of OsXTH8 was sufficient for hormone- induced OsXTH8 expression. This observation was also confirmed by GUS assay using methylumbelliferylglucuronide as a substrate (Fig. 7C).
 
−
[[File:dami4.jpg|caption]]       [[File:dami5.jpg|thumb|300px]]
 
  
 
===Evolution===
 
===Evolution===
−
The analysis revealed that XTHs could be loosely grouped into four distinct groups (Fig. 3). Group 1 contains genes that share a high level of sequence identity among different species and that are expressed in young developing tissues. Group 2 comprises ''XTH'' genes from several species showing diverse patterns of expression and response to hormonal or mechanical stimuli, including touchinducible, flooding-response, BR-inducible, and fruit ripening-related ''XTHs''. Group 3 represents a divergent group of ''XTHs'', including ''NXG1'' from nasturtium (''Tropaeolum'' ''majus'') that can act as xyloglucan hydrolase and transglycosylase. OsXTH8 has the highest homology to sequences in Group 4, consisting of well-characterized barley (''Hordeum vulgare'') genes, ''HvPM2'' and ''HvPM5'',which are up-regulated by GA in barley leaf sheaths and leaves. Monocot members of group 4 revealed to have two substitutions (DEIDIEFMG) compared to the consensus sequence (DEIDFEFLG). The presence of different amino acid residues in the putative catalytic region may attribute to unique enzymatic activity of OsXTH8.  
+
The analysis revealed that XTHs could be loosely grouped into four distinct groups (Fig. 3). Group 1 contains genes that share a high level of sequence identity among different species and that are expressed in young developing tissues<ref name="ref3"/><ref name="ref4"/><ref name="ref5"/>. Group 2 comprises ''XTH'' genes from several species showing diverse patterns of expression and response to hormonal or mechanical stimuli, including touchinducible, flooding-response, BR-inducible, and fruit ripening-related ''XTHs''<ref name="ref6"/>. Group 3 represents a divergent group of ''XTHs'', including ''NXG1'' from nasturtium (''Tropaeolum'' ''majus'') that can act as xyloglucan hydrolase and transglycosylase<ref name="ref7"/>. OsXTH8 has the highest homology to sequences in Group 4, consisting of well-characterized barley (''Hordeum vulgare'') genes, ''HvPM2'' and ''HvPM5'',which are up-regulated by GA in barley leaf sheaths and leaves<ref name="ref8"/>. Monocot members of group 4 revealed to have two substitutions (DEIDIEFMG) compared to the consensus sequence (DEIDFEFLG). The presence of different amino acid residues in the putative catalytic region may attribute to unique enzymatic activity of OsXTH8.  
  
 
[[File:rice 2.jpg|caption]]
 
[[File:rice 2.jpg|caption]]
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==References==
 
==References==
−
[1] Jan A, Yang G, Nakamura H, et al. Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice [J]. Plant physiology, 2004, 136(3): 3670-3681.
+
<references>
 +
 
 +
<ref name="ref1">Jan A, Yang G, Nakamura H, et al. Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice [J]. Plant physiology, 2004, 136(3): 3670-3681.</ref>
  
−
[2]Tanaka T, Antonio B A, Kikuchi S, et al. The rice annotation project database (RAP-DB): 2008 update[J]. Nucleic acids research, 2008, 36(Sup 1): D1028-D1033.
+
<ref name="ref2">Xu W, Campbell P, Vargheese AK, Braam J. The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression. Plant J. 1996 Jun; 9(6):879-89.</ref>
  
−
[3]Rice Annotation Project, et al. Curated genome annotation of ''Oryza sativa'' ssp. ''japonica'' and comparative genome analysis with ''Arabidopsis thaliana.'' Genome Res, 2007, 17: 175-183.  
+
<ref name="ref3">Catalá C, Rose JK, Bennett AB .Auxin regulation and spatial localization of an endo-1,4-beta-D-glucanase and a xyloglucan endotransglycosylase in expanding tomato hypocotyls.Plant J. 1997 Aug; 12(2):417-26.</ref>
 +
 +
<ref name="ref4">Shimizu Y, Aotsuka S, Hasegawa O, Kawada T, Sakuno T, Sakai F, Hayashi T. Changes in levels of mRNAs for cell wall-related enzymes in growing cotton fiber cells. Plant Cell Physiol. 1997 Mar; 38(3):375-8.</ref>
  
−
[4] Ohyanagi H, et al. The Rice Annotation Project Database (RAP-DB): hub for Oryza sativa ssp. japonica genome information. Nucleic Acids Research, 2006, 34: D741-D744.
+
<ref name="ref5">Takano M, Fujii N, Higashitani A, Nishitani K, Hirasawa T, Takahashi H. Endoxyloglucan transferase cDNA isolated from pea roots and its fluctuating expression in hydrotropically responding roots. Plant Cell Physiol. 1999 Feb; 40(2):135-42.</ref>
  
−
[5] International Rice Genome Sequencing Project. The map-based sequence of the rice genome. Nature, 2005,436(11): 793-800.
+
<ref name="ref6">Catalá C, Rose JK, York WS, Albersheim P, Darvill AG, Bennett AB. Characterization of a tomato xyloglucan endotransglycosylase gene that is down-regulated by auxin in etiolated hypocotyls. Plant Physiol. 2001 Nov; 127(3):1180-92.</ref>
  
−
[6] Asad Jan and Setsuko Komatsu. Functional Characterization of Gibberellin-Regulated Genes in Rice Using Microarray System. Geno. Prot. Bioinfo. 2006,4(3): 137-144.
+
<ref name="ref7">de Silva J, Jarman CD, Arrowsmith DA, Stronach MS, Chengappa S, Sidebottom C, Reid JS. Molecular characterization of a xyloglucan-specific endo-(1--&gt;4)-beta-D-glucanase (xyloglucan endo-transglycosylase) from nasturtium seeds. Plant J. 1993 May; 3(5):701-11.</ref>
  
 +
<ref name="ref8">Smith RC, Matthews PR, Schunmann PHD, Chandler PM(1996). The regulation of leaf elongation and xyloglucan endotransglucosylase by gibberellin in “Himalaya” barley (Hordeum vulgare L.). J Exp Bot 47: 1395–1404.</ref>
 
==Structured Information==
 
==Structured Information==
 
{{JaponicaGene|
 
{{JaponicaGene|

Latest revision as of 10:44, 9 June 2014

Please input one-sentence summary here.

Annotated Information

Function

Xyloglucan endotransglucosylases/hydrolases (XTHs) that mediate cleavage and rejoining of the β (1-4)-xyloglucans of the primary cell wall are considered to play an important role in the construction and restructuring of xyloglucan cross-links.OsXTH8 was a single copy gene; its full-length cDNA was 1,298 bp encoding a predicted protein of 290 amino acids.OsXTH8 may play cooperative role with OsXTR1, OsXTR2, OsXTR3, OsXTR4 genes and others that are expressed in overlapping regions. OsXTH8 is involved in cell wall modification processes during rice growth and development.OsXTH8 acts to alter the structure of cell wall in response to gibberellic acid[1]. .

Expression

OsXTH8 was preferentially expressed in rice leaf sheath in response to gibberellic acid. OsXTH8 was highly expressed in vascular bundles of leaf sheath and young nodal roots where the cells are actively undergoing elongation and differentiation. OsXTH8 gene expression was up-regulated by gibberellic acid and there was very little effect of other hormones.Reduced accumulation of active GA was accompanied by preferential suppression of OsXTH8 expression, and OsXTH8 expression increased when exogenous GA3 was applied to the semidwarf mutant. In two genetic mutants of rice with abnormal height, the expression of OsXTH8 positively correlated with the height of the mutants. Transgenic rice expressing an RNAi construct of OsXTH8 exhibited repressed growth. These results indicate that OsXTH8 is differentially expressed in rice leaf sheath in relation to gibberellin and potentially involved in cell elongation processes.

OsXTH8 was expressed in growing regions such as shoot apical meristem, vascular bundles of leaf sheath, and young crown roots developing from nodes; thus, OsXTH8 exhibited a unique expression pattern in terms of organ and stage specificity during leaf sheath elongation and young nodal root development. The expression of OsXTH8 in the shoot apex meristem, vascular bundles in leaf sheath, and youngnodal roots supports its role in cell wall modification processes during active growth[1].


caption

Alternative Organ- and Cell Type-Expression Profiles of OsXTH8

It has been shown that different members of XTH gene family are specifically regulated by various physiological and environmental stimuli[2].To examine the tissue specificity of OsXTH8, total RNAs from rice callus, root, leaf blade, and leaf sheath were hybridized with OsXTH8-specific DNA probe. A strong signal was detected in leaf sheaths but weak or no signal was observed in leaf blades, roots, and calli (Fig. 4A). These expressions were enhanced by GA3 treatment (Fig. 4A). When 1-month-old seedlings were used to characterize the expression in leaf sheath, the expression was mainly found in the three basal parts of leaf sheath (Fig. 4B). Enhanced expression of OsXTH8 in the third part of 1-month-old rice seedling, which corresponded to the second internode of leaf sheath, compared to two basal parts of leaf sheath showed that OsXTH8 is differentially expressed in leaf sheath[1].

caption caption caption

Evolution

The analysis revealed that XTHs could be loosely grouped into four distinct groups (Fig. 3). Group 1 contains genes that share a high level of sequence identity among different species and that are expressed in young developing tissues[3][4][5]. Group 2 comprises XTH genes from several species showing diverse patterns of expression and response to hormonal or mechanical stimuli, including touchinducible, flooding-response, BR-inducible, and fruit ripening-related XTHs[6]. Group 3 represents a divergent group of XTHs, including NXG1 from nasturtium (Tropaeolum majus) that can act as xyloglucan hydrolase and transglycosylase[7]. OsXTH8 has the highest homology to sequences in Group 4, consisting of well-characterized barley (Hordeum vulgare) genes, HvPM2 and HvPM5,which are up-regulated by GA in barley leaf sheaths and leaves[8]. Monocot members of group 4 revealed to have two substitutions (DEIDIEFMG) compared to the consensus sequence (DEIDFEFLG). The presence of different amino acid residues in the putative catalytic region may attribute to unique enzymatic activity of OsXTH8.

caption

Labs working on this gene

National Institute of Agrobiological Sciences, Tsukuba 305–8602, Japan (A.J., G.Y., H.N., H.I., S.K.);

University of Tsukuba, Tsukuba 305–8572, Japan (A.J., H.M.);

Nagoya University, Nagoya 464–8601, Japan (H.K., M.M.);

National Institute of Crop Science, Tsukuba 305-8518, Japan.

References

<references>

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

Structured Information

Gene Name

Os08g0237000

Description

Xyloglucan endotransglycosylase/hydrolase protein 8 precursor (EC 2.4.1.207) (End-xyloglucan transferase) (OsXTH8) (OsXRT5)

Version

NM_001067854.1 GI:115475444 GeneID:4345019

Length

1378 bp

Definition

Oryza sativa Japonica Group Os08g0237000, complete gene.

Source

Oryza sativa Japonica Group 

 ORGANISM  Oryza sativa Japonica Group
           Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
           Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
           clade; Ehrhartoideae; Oryzeae; Oryza.
Chromosome

Chromosome 8

Location

Chromosome 8:8327025..8328402

Sequence Coding Region

8327064..8327545,8327644..8328034

Expression

GEO Profiles:Os08g0237000

Genome Context

<gbrowseImage1> name=NC_008401:8327025..8328402 source=RiceChromosome08 preset=GeneLocation </gbrowseImage1>

Gene Structure

<gbrowseImage2> name=NC_008401:8327025..8328402 source=RiceChromosome08 preset=GeneLocation </gbrowseImage2>

Coding Sequence

<cdnaseq>atggcgaagcatctcgcgctgtccgtggccgccgcggtggccgtgtcgtggctggcggcgtcgtcggcggcggcggcggggttctacgagaagttcgacgtggtgggcgccggcgaccacgtgagggtggtgagcgacgacgggaagacgcagcaggtggcgctgacgctggaccggagctccgggtccgggttcacctccaaggacacctacctgttcggcgagttcagcgtccagatgaagctcgtcggcggcaactccgccggcaccgtcacctccttctacctctcctccggcgagggcgacggccacgacgagatcgacatcgagttcatgggcaacctcagcggcaacccctacgtcatgaacaccaacgtctgggctaatggcgacggcaagaaggagcaccagttctacctctggttcgaccccaccgccgacttccacacctacaagatcatctggaatccccaaaacatcatattccaggtggacgacgtgccggtgaggacgttcaagaagtacgacgacctggcgtacccgcagagcaagccgatgaggctgcacgcgacgctgtgggacggcagctactgggcgacgaggcacggcgacgtcaagatcgactggagcggcgcgccgttcgtggtgtcgtaccgcgggtacagcaccaacgcgtgcgtcaacaacaatcccgccggcgggtggtcgtcgtcgtggtgccccgagggcacgtcggcgtggatccaccgcgagctcgacggcgccgagctcggcaccgtcgcgtgggccgagcgcaactacatgtcctacaactactgcgccgacggctggcgcttcccccagggcttccccgccgagtgctaccgcaagtga</cdnaseq>

Protein Sequence

<aaseq>MAKHLALSVAAAVAVSWLAASSAAAAGFYEKFDVVGAGDHVRVV SDDGKTQQVALTLDRSSGSGFTSKDTYLFGEFSVQMKLVGGNSAGTVTSFYLSSGEGD GHDEIDIEFMGNLSGNPYVMNTNVWANGDGKKEHQFYLWFDPTADFHTYKIIWNPQNI IFQVDDVPVRTFKKYDDLAYPQSKPMRLHATLWDGSYWATRHGDVKIDWSGAPFVVSY RGYSTNACVNNNPAGGWSSSWCPEGTSAWIHRELDGAELGTVAWAERNYMSYNYCADG WRFPQGFPAECYRK</aaseq>

Gene Sequence

<dnaseqindica>40..521#620..1010#atccaccacccaaagacaaagcaagcaagtacagtagccatggcgaagcatctcgcgctgtccgtggccgccgcggtggccgtgtcgtggctggcggcgtcgtcggcggcggcggcggggttctacgagaagttcgacgtggtgggcgccggcgaccacgtgagggtggtgagcgacgacgggaagacgcagcaggtggcgctgacgctggaccggagctccgggtccgggttcacctccaaggacacctacctgttcggcgagttcagcgtccagatgaagctcgtcggcggcaactccgccggcaccgtcacctccttctacctctcctccggcgagggcgacggccacgacgagatcgacatcgagttcatgggcaacctcagcggcaacccctacgtcatgaacaccaacgtctgggctaatggcgacggcaagaaggagcaccagttctacctctggttcgaccccaccgccgacttccacacctacaagatcatctggaatccccaaaacatcatgtacgcatcacaaaaacgaactcaataatcctctcttcgtcaatcaaacaatcatcgtgttaatttgctgaattacgattacttccgctatgatacagattccaggtggacgacgtgccggtgaggacgttcaagaagtacgacgacctggcgtacccgcagagcaagccgatgaggctgcacgcgacgctgtgggacggcagctactgggcgacgaggcacggcgacgtcaagatcgactggagcggcgcgccgttcgtggtgtcgtaccgcgggtacagcaccaacgcgtgcgtcaacaacaatcccgccggcgggtggtcgtcgtcgtggtgccccgagggcacgtcggcgtggatccaccgcgagctcgacggcgccgagctcggcaccgtcgcgtgggccgagcgcaactacatgtcctacaactactgcgccgacggctggcgcttcccccagggcttccccgccgagtgctaccgcaagtgattttgaactcgatcgattcaaatcctcctccattgatgagttcttggcaatgatttgtaattgcttcttgttcttgttttcgtcttcgtcttcgtcttcttcttcttgatccatgtacattttgccatccattcgttctccatttgttacagttacagagacaggttgatggtgaattactattgccgccaatttttttcttcttcttgttactcgatccaattattactactagagttcatcttgcacagttgggcggtgtgaaaaactgaaaaagaaaagggtgtcaaatttgttgctgatgctgagcaagtgagcacatgcttatgctctcaggtatgaacaaaataaacagtaaaattatctgc</dnaseqindica>

External Link(s)

NCBI Gene:Os08g0237000, RefSeq:Os08g0237000

  1. ↑ 1.0 1.1 1.2 1.3 Jan A, Yang G, Nakamura H, et al. Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice [J]. Plant physiology, 2004, 136(3): 3670-3681.
  2. ↑ 2.0 2.1 Xu W, Campbell P, Vargheese AK, Braam J. The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression. Plant J. 1996 Jun; 9(6):879-89.
  3. ↑ 3.0 3.1 Catalá C, Rose JK, Bennett AB .Auxin regulation and spatial localization of an endo-1,4-beta-D-glucanase and a xyloglucan endotransglycosylase in expanding tomato hypocotyls.Plant J. 1997 Aug; 12(2):417-26.
  4. ↑ 4.0 4.1 Shimizu Y, Aotsuka S, Hasegawa O, Kawada T, Sakuno T, Sakai F, Hayashi T. Changes in levels of mRNAs for cell wall-related enzymes in growing cotton fiber cells. Plant Cell Physiol. 1997 Mar; 38(3):375-8.
  5. ↑ 5.0 5.1 Takano M, Fujii N, Higashitani A, Nishitani K, Hirasawa T, Takahashi H. Endoxyloglucan transferase cDNA isolated from pea roots and its fluctuating expression in hydrotropically responding roots. Plant Cell Physiol. 1999 Feb; 40(2):135-42.
  6. ↑ 6.0 6.1 Catalá C, Rose JK, York WS, Albersheim P, Darvill AG, Bennett AB. Characterization of a tomato xyloglucan endotransglycosylase gene that is down-regulated by auxin in etiolated hypocotyls. Plant Physiol. 2001 Nov; 127(3):1180-92.
  7. ↑ 7.0 7.1 de Silva J, Jarman CD, Arrowsmith DA, Stronach MS, Chengappa S, Sidebottom C, Reid JS. Molecular characterization of a xyloglucan-specific endo-(1-->4)-beta-D-glucanase (xyloglucan endo-transglycosylase) from nasturtium seeds. Plant J. 1993 May; 3(5):701-11.
  8. ↑ 8.0 8.1 Smith RC, Matthews PR, Schunmann PHD, Chandler PM(1996). The regulation of leaf elongation and xyloglucan endotransglucosylase by gibberellin in “Himalaya” barley (Hordeum vulgare L.). J Exp Bot 47: 1395–1404.
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