Difference between revisions of "Os01g0228600"
(→Function) |
(→Annotated Information) |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 2: | Line 2: | ||
==Annotated Information== | ==Annotated Information== | ||
| + | [[File:Os02g0101500.png|right|thumb|327px|'''Figure 4.''' ''Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.<ref name="ref1" />.'']] | ||
===Gene Symbol=== | ===Gene Symbol=== | ||
*'''''Os01g0228600''''' '''<=>''' '''''OsHPR2''''','''''HPR2''''' | *'''''Os01g0228600''''' '''<=>''' '''''OsHPR2''''','''''HPR2''''' | ||
===Function=== | ===Function=== | ||
* The researchers constructed over-expression of the '''''OsHPR1''''' gene and RNA interference plants of '''''OsHPR1''''' and '''''OsHPR2''''' genes in rice. | * The researchers constructed over-expression of the '''''OsHPR1''''' gene and RNA interference plants of '''''OsHPR1''''' and '''''OsHPR2''''' genes in rice. | ||
| − | |||
* The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line. | * The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line. | ||
| + | * Double deletion of '''''OsHPR1''''' and '''''OsHPR2''''' genes significantly reduces plant growth under normal photorespiration condition | ||
* These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. | * These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice. | ||
===Expression=== | ===Expression=== | ||
| − | + | * The expression of HPR1 gene is induced by light. | |
| + | * The light regulations on HPR1 gene expression and alternative splicing were species-specific. | ||
| + | * Results from RT-PCR, western-blot and enzyme assays showed that '''''HPR1''''' activity changed significantly in corresponding transgenic lines without any effect on '''''HPR2''''' activity, which is the same for HPR2. | ||
| − | === | + | ===Subcellular Localization=== |
| − | + | * In the present study, the subcellular localization of '''''OsHPR2''''' protein was first predicted to be located in the peroxisome and cytosol respectively. | |
| + | * The fluorescent pattern of chimeric protein with HPR2 was found to accumulate in the cytosol and on the surface of the nucleus. | ||
You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
Latest revision as of 13:10, 3 October 2016
The rice Os01g0228600 was reported as OsHPR2 in 2014 [1] by researchers from China.
Contents
Annotated Information
Figure 4. Subcellular localizations of GFP–HPR1 and GFP-HPR2 fusions in transgenic rice protoplasts.[1].
Gene Symbol
- Os01g0228600 <=> OsHPR2,HPR2
Function
- The researchers constructed over-expression of the OsHPR1 gene and RNA interference plants of OsHPR1 and OsHPR2 genes in rice.
- The phenotypes of all transgenic lines were determined in ambient air and CO 2 -elevated air. The phenotype typical of photorespiration mutants were observed only where activity of both HPR1 and HPR2 were down-regulated in the same line.
- Double deletion of OsHPR1 and OsHPR2 genes significantly reduces plant growth under normal photorespiration condition
- These findings demonstrate that two hydroxypyruvate reductases encoded by OsHPR1 and OsHPR2 are involved in photorespiratory metabolism in rice.
Expression
- The expression of HPR1 gene is induced by light.
- The light regulations on HPR1 gene expression and alternative splicing were species-specific.
- Results from RT-PCR, western-blot and enzyme assays showed that HPR1 activity changed significantly in corresponding transgenic lines without any effect on HPR2 activity, which is the same for HPR2.
Subcellular Localization
- In the present study, the subcellular localization of OsHPR2 protein was first predicted to be located in the peroxisome and cytosol respectively.
- The fluorescent pattern of chimeric protein with HPR2 was found to accumulate in the cytosol and on the surface of the nucleus.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, China,
- The Chinese University of Hong Kong Shenzhen Institute, Shenzhen, China,
- State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China
