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| − | {{JaponicaGene|
| + | The rice '''''Os07g0176600''''' was reported as '''''OsNek3''''' in 2009 <ref name="ref1" /> by researchers from Japan. |
| − | GeneName = Os07g0176600|
| |
| − | Description = Similar to Kinase-like protein|
| |
| − | Version = NM_001065557.1 GI:115470846 GeneID:4342542|
| |
| − | Length = 4740 bp|
| |
| − | Definition = Oryza sativa Japonica Group Os07g0176600, complete gene.|
| |
| − | Source = Oryza sativa Japonica Group
| |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| + | ==Annotated Information== |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| + | ===Gene Symbol=== |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| + | *'''''Os07g0176600''''' '''''<=>''''' '''''OsNek3, OsSTA192''''' |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| + | |
| − | |
| + | ===Function=== |
| − | Chromosome = [[:category:Japonica Chromosome 7|Chromosome 7]]|
| + | * '''''OsNek3''''' might play a role in pollen germination. |
| − | AP = Chromosome 7:4081936..4086675|
| + | * '''''OsNek3''''' is downstream of DCW11 in retrograde signaling from the mitochondria to the nucleus and is involved in CW-CMS. |
| − | CDS = 4082672..4082757,4082871..4082937,4083424..4083489,4083628..4083674,4083757..4083802<br>,4083905..4083985,4084105..4084147,4084258..4084307,4084392..4084473<br>,4084538..4084587,4084687..4084760,4084870..4084921,4085034..4085459<br>,4085816..4086403|
| + | |
| − | GCID = <gbrowseImage1>
| + | ===Phenotypic analysis=== |
| − | name=NC_008400:4081936..4086675
| + | * A knockout mutant with Tos17 inserted into '''''OsNek3''''' did not show any pollen-defective phenotype. |
| − | source=RiceChromosome07
| + | * On the other hand, plants overexpressing '''''OsNek3''''' occasionally produced a peculiar pollen structure in which the outer cell wall of four pollen grains fused together even at the mature pollen stages, which resembled that of quartet mutants in Arabidopsis. |
| − | preset=GeneLocation
| + | * '''''OsNek3''''' was shown to interact with a LIM domain-containing protein, '''''OsPLIM2b''''', whose expression was strongly specific in mature pollen |
| − | </gbrowseImage1>| | + | * '''''OsNek3''''' was shown to be down-regulated in DCW11-knockdown lines, whereas osnek3 mutation did not result in DCW11 down-regulation. |
| − | GSID = <gbrowseImage2>
| + | |
| − | name=NC_008400:4081936..4086675
| + | ===Expression=== |
| − | source=RiceChromosome07
| + | * '''''OsNek3''''' was preferentially expressed in mature pollen. |
| − | preset=GeneLocation
| + | |
| − | </gbrowseImage2>| | + | You can also add sub-section(s) at will. |
| − | CDNA = <cdnaseq>atggagcagtatgaggtgctggagcagattgggaaggggtcatttggctctgccctcctggtgagacacaaggttgagaagaagaggtatgtcttgaagaagatcagacttgcccgtcagaccgacagatgccgccgatcagcacaccaagagatggagctcattgcaaaagtaaggaacccttacattgtggaatacaaagattcatgggtggagaagggttgttatgtgtgcatcgtgattggttactgtgaaggaggagacatgtcagaagccattaagaaggccaacagtaattatttctcagaagagagactttgtatgtggcttgtgcagctcctgatggcacttgattacttgcatgtcaaccatatccttcatcgtgatgtgaagtgctcaaatatatttctgacgaaggatcaaaacatacggctcggtgattttggtttggccaaagtattgacttctgacgatttaacttcatcagttgtgggtactcccagctacatgtgccctgaacttcttgctgacattccatatggttccaagtctgacatatggtcactaggctgttgcctctatgagatgactgctttgaagcctgcatttaaagcatttgatatgcaaacactgataaacaagataagcaagtcggttctcgctcctctaccgaccatctactccggtgcatttagggggctcatcaagagcatgctgcgaaaaagtccagatcacagaccaagtgctgcagaattgcttaagcacccacaccttcagccctttgtactggaactccaattgaagtcgagtcccgctcgcaatcttttcccggatacaaataaggcttcatgttctgatgatgaaaacaattggaaggctaagtacagcaagagccattcatttaaagtagacaggattgtaaaagttgacaaagttgctgctaacaatggccatcctagttctactggaactgcaaaagattaccaagaattgctaaaacaaccaatggatgagttgttaggacaattaaccgagaaagttgttgatgaagtgatacatggaaatcattcaagagtaacaaaatctccagctcctactccaagaagggcatcatcaactcctagaatacgactagaaccttcaaagacatttcatgctagagcagctgagactccaccatccaaatgttcactggaacgagcaagtcaacctacaagacgagcatcaaccccggtgaacatgttgcaaactcctgagaaacggcagggcgccgacatcctcaccagactaaaatcacccgacgtttcagtgaactctcctcgaatcgacagaatcgccgaattcccgataccatcatttgatgatgaacaattgcaccccacaaccaagctcaagctctatccaccatccatcactgaccaatcgatcaccaaggacaagtgcacattccaggttctccgaagcgacagcagcaagaaccacaccggagacagttctgatcccagcattcttggcactgacagtaatccactcatcacgagctcatcggattggatgaagcagaggagattcgacacgacgtcgtaccgtcagagagccgaggcactggaaggattgctggagttcagtgcacagctgctgcagcaggagaggtttgaggagctggggatcttgctgaagccatttgggccagggaaggcgtctccgagagagacggccatatggttgtcaaagagcttcaaagggactggattgtga</cdnaseq>|
| + | |
| − | AA = <aaseq>MEQYEVLEQIGKGSFGSALLVRHKVEKKRYVLKKIRLARQTDRC RRSAHQEMELIAKVRNPYIVEYKDSWVEKGCYVCIVIGYCEGGDMSEAIKKANSNYFS EERLCMWLVQLLMALDYLHVNHILHRDVKCSNIFLTKDQNIRLGDFGLAKVLTSDDLT SSVVGTPSYMCPELLADIPYGSKSDIWSLGCCLYEMTALKPAFKAFDMQTLINKISKS VLAPLPTIYSGAFRGLIKSMLRKSPDHRPSAAELLKHPHLQPFVLELQLKSSPARNLF PDTNKASCSDDENNWKAKYSKSHSFKVDRIVKVDKVAANNGHPSSTGTAKDYQELLKQ PMDELLGQLTEKVVDEVIHGNHSRVTKSPAPTPRRASSTPRIRLEPSKTFHARAAETP PSKCSLERASQPTRRASTPVNMLQTPEKRQGADILTRLKSPDVSVNSPRIDRIAEFPI PSFDDEQLHPTTKLKLYPPSITDQSITKDKCTFQVLRSDSSKNHTGDSSDPSILGTDS NPLITSSSDWMKQRRFDTTSYRQRAEALEGLLEFSAQLLQQERFEELGILLKPFGPGK ASPRETAIWLSKSFKGTGL</aaseq>|
| + | ==Labs working on this gene== |
| − | DNA = <dnaseqindica>737..822#936..1002#1489..1554#1693..1739#1822..1867#1970..2050#2170..2212#2323..2372#2457..2538#2603..2652#2752..2825#2935..2986#3099..3524#3881..4468#agccctccattagcaagagtgcccaatctatctgtcttctccataagagagcatctcaggtgtagtttttagtttctctctctaaaaatgggaggcaactgaggaagaaggaagaacacggccttcatttcatccaattgagtcaaggtagagattcttctggcttttcttctcttcttcctctccaaatatcaatgctgcttcaagcttttccaagaatcgatgtgccttcagattaatgttcttctggcttttgtttttttctctcttctttttcaagcatctgtatgattgtttttcatgctttcttcttctcttcattgcttgttcttcatctgttccttccctaaatgctgtcctctagtgtgaaattaggaattgcaaccgaattttgcaaattgatatcaacttcgcatttacatagctattaattgcacttcttattgtcctttatgtcctcctttcttgattatgacttgtgaattttcatgaggcaaatccattgccaatgttctttcaagctgaaagcaaagcattcttggctcatctgttccctatccaagaaccctcaatggattttgaattttttgaatggcatgacatctcgagccatttcaaaaactagtatttggctgggccacatttgaacagtttcacagaaatttcttgctgttcccttcaaaatcatatcattttcatcgcagagaggaaatctgaatttgggggcggccaagaaatggagcagtatgaggtgctggagcagattgggaaggggtcatttggctctgccctcctggtgagacacaaggttgagaagaagaggtaaggcagatactatagtcttcttccttcttgtgcaatgcatacattgctttctaggattttgcaagatgatgttaccctccaaaaaatctgaaaaaaaaggattgtttcaggtatgtcttgaagaagatcagacttgcccgtcagaccgacagatgccgccgatcagcacaccaagaggtccccttaacaccctctgtcccctttggggagtgtgatatcttacaattcaagttagcttaagttgtgaaaaaggttctattattgttcatgttttggggtactgttcttgtggtcctgtttctgaattcctaatttgaaatgccgcaatcatatccacgctggtaatcataggtaagttagacggcaaatgcaaaatggctttagattttgttgatttattgcgcaatgattttattgaaaaagattgttctttcgatatacccaaaaaaaaaaacatgagaggatttgaatattcaaatgggtcaaacttatttgttgtcttccagggcctgctactctgtttccatggaccatggcagactgtacttttgtcatggctttcttgtgataatacagaaatgatggtttctgtcatgatctagtgctaatgcagatatcgaccttaatcatgtgcataacttaatgattttttttctcatacagatggagctcattgcaaaagtaaggaacccttacattgtggaatacaaagattcatgggtggagaaggtgagcaattgagcatggtccctctctcctagctgtgcatattggcaggatcccaatatcaaatcttttctttgaatggtcctacaaaaaaatggtccagatgttgttcattgtttaaaatgattttgttgtcttcagggttgttatgtgtgcatcgtgattggttactgtgaaggaggagacatgtatgtaacttgtgacaataagctctgaaatgtccctggaatttgactttgaattctgataatttgcactctttttgtgtaggtcagaagccattaagaaggccaacagtaattatttctcagaagaggttgttgtctatactctctagagcatatgactttgtttttttaccggtggattctgattactgaaatttgcacctttgtgctgtccatttctctgcatgcagagactttgtatgtggcttgtgcagctcctgatggcacttgattacttgcatgtcaaccatatccttcatcgtgatgtgaaggtttgatttgttatattcataatctttatcatctcaaaaaaaatatattcataacctttatgctcttacaatggactggttctgcttacttatcatattttgctgtgtcattctttcagtgctcaaatatatttctgacgaaggatcaaaacatacggctcggtaagtatcctcttgtaaattcagtaaatgaaaaaatgttttaaacattttacttcaaataaatgatgttaatatcatcctggttaattggaatatgatttctgaaacaggtgattttggtttggccaaagtattgacttctgacgatttaacttcatcagtaagttgctcttgctctacactgtagttgattctctgtctaaattgcttctgattgtgatatcataatgcattccttctgaaggttgtgggtactcccagctacatgtgccctgaacttcttgctgacattccatatggttccaagtctgacatatggtcactaggtaagccttcttctgaaatctgaatgttgcaaagttctcctggttaaacatctgaaatttccaggctgttgcctctatgagatgactgctttgaagcctgcatttaaagcatttgtaagtagctatgaaagaaaaaaccaaataatgccattttgtactcattttaccaaaatttctaccttagatgaatgatgaatctatcatttgtgacaggatatgcaaacactgataaacaagataagcaagtcggttctcgctcctctaccgaccatctactccggtgcattgtaagccctttgatccacagtaacttatgtgagatcataatagaattttgaacctaatccttttgtcatctctgaaattttcatgccaaatttgttttggttaatacagtagggggctcatcaagagcatgctgcgaaaaagtccagatcacagaccaagtgtaagtgttccttccaaatcagcatattattttcaacctcacttttcattagtactctggtttctgaactcattcatgcatctcacagcctgatgtttatgctgtttttcaggctgcagaattgcttaagcacccacaccttcagccctttgtactggaactccaattgaagtcgagtcccgctcgcaatcttttcccggatacaaataaggcttcatgttctgatgatgaaaacaattggaaggctaagtacagcaagagccattcatttaaagtagacaggattgtaaaagttgacaaagttgctgctaacaatggccatcctagttctactggaactgcaaaagattaccaagaattgctaaaacaaccaatggatgagttgttaggacaattaaccgagaaagttgttgatgaagtgatacatggaaatcattcaagagtaacaaaatctccagctcctactccaagaagggcatcatcaactcctagaatacgactagaaccttcaaagacatttcatgctagagcagctgaggtatgaagaaaatttcttttcttaagaactgtttgattttttttccagtttggttgagaggtctgaaaagttaatatgaatcatagttccatttttgttctctcagcatacaaaatattaacatataatcatcactaacaagaaaaaccagtaattgttcatgatgaattttaccaaaaagtatttctatcaaacaaagtgaccattcattctagctgccattgtaaatgtatgtttgaaatgcttttatacctgtcatgtggcaagtgcatgatttgaatcatcttgtgtatctgaagaaaatatcacacacataaaattctgacattcttttgctttgacatcaacactgacagactccaccatccaaatgttcactggaacgagcaagtcaacctacaagacgagcatcaaccccggtgaacatgttgcaaactcctgagaaacggcagggcgccgacatcctcaccagactaaaatcacccgacgtttcagtgaactctcctcgaatcgacagaatcgccgaattcccgataccatcatttgatgatgaacaattgcaccccacaaccaagctcaagctctatccaccatccatcactgaccaatcgatcaccaaggacaagtgcacattccaggttctccgaagcgacagcagcaagaaccacaccggagacagttctgatcccagcattcttggcactgacagtaatccactcatcacgagctcatcggattggatgaagcagaggagattcgacacgacgtcgtaccgtcagagagccgaggcactggaaggattgctggagttcagtgcacagctgctgcagcaggagaggtttgaggagctggggatcttgctgaagccatttgggccagggaaggcgtctccgagagagacggccatatggttgtcaaagagcttcaaagggactggattgtgattaatcagagtcctgcaaatgcaaaaaaaagaaaaaaaaatctgaacctagaataattttgtgtctttgattagattcaggctgctctgtacagtttcagttttgtggtaatatcctcttttcgtctttgattttcgcgaaatgtttgaaacactccgttgtttggcgagagaagctccatttttgtgtatagcaaataattgctcaagaatggggaagcaccggtggtgtggtggtgaaatcaggaatgaatttactccgtattctgaggg</dnaseqindica>|
| + | * Laboratory of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001065557.1 RefSeq:Os07g0176600]|
| + | |
| − | }}
| + | ==References== |
| − | [[Category:Genes]] | + | <references> |
| − | [[Category:Japonica mRNA]]
| + | * <ref name="ref1"> |
| − | [[Category:Oryza Sativa Japonica Group]] | + | Fujii S, Yamada M, Toriyama K. Cytoplasmic male sterility-related protein |
| − | [[Category:Japonica Genes]] | + | kinase, OsNek3, is regulated downstream of mitochondrial protein phosphatase 2C, |
| − | [[Category:Japonica Chromosome 7]]
| + | DCW11. Plant Cell Physiol. 2009 Apr;50(4):828-37. doi: 10.1093/pcp/pcp026. PubMed |
| − | [[Category:Chromosome 7]]
| + | PMID: 19224952. |
| | + | |
| | + | </ref> |
| | + | </references> |
| | + | |
| | + | ==Structured Information== |
| | + | [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 7]] |
