Difference between revisions of "Os04g0499300"
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Please input one-sentence summary here. | Please input one-sentence summary here. | ||
| + | The rice Rymv1 gene is well known as the "green revolution gene" and controls the plant height of rice. | ||
==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
Revision as of 00:41, 20 May 2014
Please input one-sentence summary here.
The rice Rymv1 gene is well known as the "green revolution gene" and controls the plant height of rice.
Contents
Annotated Information
Function
Rymv1，抗水稻黄斑驳病毒病基因，编码真核翻译起始因子异构体eIF(iso)4G。
【基因的定位】
Rymv1 初步定位于水稻第4 染色体上SSR标记RM252 和AFLP标记E-ACG/M-ACA 之间的3.7cM 区间内(Albar et al.,2003)。后通过发掘新标记、构建物理图谱，将Rymv1 定位在BAC克隆OSJNBa0067K08 和OSJNBa0029H02 之间的160kb 区间内，并最终在OSJNBa0029H02 上找到Rymv1 的候选基因(Albar et al., 2006)。
Rymv1 定位在水稻第4 染色体上，对应于日本晴测序图谱的位置(5'-3')在24766929 - 24761516 区间(Rice Genome Annotation Project)。
【基因的克隆与生物学功能分析】
Rymv1 包含9个外显子和8个内含子，编码真核翻译起始因子异构体eIF(iso)4G。在IR64和Gigante中，Rymv1 cDNA 编码区长2379bp，编码一个793氨基酸组成的蛋白，其中，第208~435 氨基酸区间对应MIF4G 功能域；第628~740 氨基酸区间对应MA3 功能域。与感病亲本的编码产物相比，抗病蛋白在MIF4G 域发生了3氨基酸的缺失或单氨基酸的置换，这些变异位于抗病蛋白三维结构的表面，暗示MIF4G 域可能参与了蛋白与病毒的交互识别作用(Albar et al., 2006)。
在植物中eIF4E 和eIF4G 形成eIF4F 复合体，而eIF(iso)4E 和eIF(iso)4G 形成eIF(iso)4F 复合体，这两个复合体参与了蛋白翻译起始步骤的mRNA 帽子结构的固定和核糖体的招募。
【备注】
水稻黄斑驳病毒病，主要发生在西非和中非的十多个国家，如肯尼亚。主要由叶甲类(Apophylias spp.)、跳甲类(Chaetoenema spp.)、小叶甲(Sesselia passilla)和非洲铁甲虫(Trichispa sericeas)作半持久性传播，亦可通过汁液接种。
Expression
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Evolution
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Labs working on this gene
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References
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Structured Information
| Gene Name |
Os04g0499300 |
|---|---|
| Description |
Initiation factor eIF-4 gamma, middle domain containing protein |
| Version |
NM_001059754.2 GI:297602993 GeneID:4336300 |
| Length |
2548 bp |
| Definition |
Oryza sativa Japonica Group Os04g0499300, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 4:25344788..25347335 |
| Sequence Coding Region |
25345201..25346256,25346777..25347043,25347116..25347235 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008397:25344788..25347335 source=RiceChromosome04 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008397:25344788..25347335 source=RiceChromosome04 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggagagaagggacaaagaaaggattgtcaaactaagaacacttggaaatatccgtctaattggtgagctacttaagcaaaagatggtacctgaaaagatagttcatcacattgttcaggaactcttggggtctggacctgataaaaaggcttgccctgaagaggaaaacgttgaggctatttgtcagttcttcaatacaattggtaaacagcttgacgagaacccaaaatctcgtcgaattaatgatacctacttcattcagatgaaggagttaacaacaaaccttcagttggcaccacgtctgaggtttatggttcgtgatgtggttgatcttcgttcaaacaattgggtgcctcgacgtgaagaggtaaataaaatcaccctaattaaggccaagacaatcagtgaaattcatgatgaggctatgaagacacttggactacggccaggggcaacaggactcacgaggaatggccgtaatgcaccaggtggtcctctttcccctggtggattcccaatgaatcgaccaggaacaggagggatgatgcctgggatgcctggaacacctggtatgcctggatcaagaaagatgcctggaatgcctgggttagataatgataactgggaagttccacgttctaaatcaatgccaagaggcgactctcttcgtaaccagggtccgttgcttaataagccatcatctattaacaagccatcatctattaactccaggcttcttcctcatggaagtggagcactaattggtaagagtgctcttttgggcagtggtggtccaccatctcgcccttccagccttatggctagtctcactcatacaccagctcaaactgcaccatcaccaaaacctgtaagtgcagctccagctgttgtgcctgtcacagacaaagcagccggttcttcccatgagatgccagctgcagttcagaagaagacggtatcccttcttgaagagtattttggcatacgtattctggatgaagcacaacaatgtatcgaggagcttcagtgtcctgagtactactctgagattgtgaaagaagctatcaatcttgctttggataagggtcccaacttcattgatcctcttgttaggctgctggagcatctgcacgccaagaaaattttcaagactgaggacctgaaaactggatgcttactgtatgcagccctgctggaagatattggtattgaccttcctctagctccagccttgtttggtgaagttgttgcacgactgagtttgtcgtgtggcttgagctttgaagttgttgaggagattctaaaagcagtggaggatacatacttccgcaaaggaatttttgatgctgtcatgaaaaccatgggcggaaactcttcaggtcaggctatattgagctcgcatgctgtggtaatcgacgcctgcaacaaacttttgaaataa</cdnaseq> |
| Protein Sequence |
<aaseq>MERRDKERIVKLRTLGNIRLIGELLKQKMVPEKIVHHIVQELLG SGPDKKACPEEENVEAICQFFNTIGKQLDENPKSRRINDTYFIQMKELTTNLQLAPRL RFMVRDVVDLRSNNWVPRREEVNKITLIKAKTISEIHDEAMKTLGLRPGATGLTRNGR NAPGGPLSPGGFPMNRPGTGGMMPGMPGTPGMPGSRKMPGMPGLDNDNWEVPRSKSMP RGDSLRNQGPLLNKPSSINKPSSINSRLLPHGSGALIGKSALLGSGGPPSRPSSLMAS LTHTPAQTAPSPKPVSAAPAVVPVTDKAAGSSHEMPAAVQKKTVSLLEEYFGIRILDE AQQCIEELQCPEYYSEIVKEAINLALDKGPNFIDPLVRLLEHLHAKKIFKTEDLKTGC LLYAALLEDIGIDLPLAPALFGEVVARLSLSCGLSFEVVEEILKAVEDTYFRKGIFDA VMKTMGGNSSGQAILSSHAVVIDACNKLLK</aaseq> |
| Gene Sequence |
<dnaseqindica>1080..2135#293..559#101..220#tacattcaagcgtgtgctgttgaacaattgtcaggaagcatttgaaggtgctgagagcctaagggctgaaatagcaaaattgactggccctgaccaagagatggagagaagggacaaagaaaggattgtcaaactaagaacacttggaaatatccgtctaattggtgagctacttaagcaaaagatggtacctgaaaagatagttcatcacattgttcaggtatgcattagcaaattggtaataaagttattattattcaggggtagcttgtctaatctgttctatatgcaggaactcttggggtctggacctgataaaaaggcttgccctgaagaggaaaacgttgaggctatttgtcagttcttcaatacaattggtaaacagcttgacgagaacccaaaatctcgtcgaattaatgatacctacttcattcagatgaaggagttaacaacaaaccttcagttggcaccacgtctgaggtttatggttcgtgatgtggttgatcttcgttcaaacaattgggtgcctcgacgtgaagaggtaaataaaatcaccctagtgttttattttagttatcttttatacatgcattgtctttctttcataatgacgttagtctcttggttgtaccgaggtgctgctatgagtactttggtcaatggctaccaaattctagcaccatagttatctgtagtggctctttaactgtgagcttattagataaacatgcatgctcatgtgggaatggcaagcaatcgccagtgctatgaaattgcatatatttatttagaatcaacgggtttgtctattgctttgtacacccctatattaataagagaatggccatgtttataatccatatttgaaataattcatcagttcaccgttttgactatacattgaagcattctgtttggtaccataatgttgtttctggcttacactaaatttaatgccaagtatggcttcaatgtttttgttcccactcttagtttgctgtttgtattagcaatcactggttagctggatttataaatttattatccattctgagttttgtgatgcttgcaatcttcagattaaggccaagacaatcagtgaaattcatgatgaggctatgaagacacttggactacggccaggggcaacaggactcacgaggaatggccgtaatgcaccaggtggtcctctttcccctggtggattcccaatgaatcgaccaggaacaggagggatgatgcctgggatgcctggaacacctggtatgcctggatcaagaaagatgcctggaatgcctgggttagataatgataactgggaagttccacgttctaaatcaatgccaagaggcgactctcttcgtaaccagggtccgttgcttaataagccatcatctattaacaagccatcatctattaactccaggcttcttcctcatggaagtggagcactaattggtaagagtgctcttttgggcagtggtggtccaccatctcgcccttccagccttatggctagtctcactcatacaccagctcaaactgcaccatcaccaaaacctgtaagtgcagctccagctgttgtgcctgtcacagacaaagcagccggttcttcccatgagatgccagctgcagttcagaagaagacggtatcccttcttgaagagtattttggcatacgtattctggatgaagcacaacaatgtatcgaggagcttcagtgtcctgagtactactctgagattgtgaaagaagctatcaatcttgctttggataagggtcccaacttcattgatcctcttgttaggctgctggagcatctgcacgccaagaaaattttcaagactgaggacctgaaaactggatgcttactgtatgcagccctgctggaagatattggtattgaccttcctctagctccagccttgtttggtgaagttgttgcacgactgagtttgtcgtgtggcttgagctttgaagttgttgaggagattctaaaagcagtggaggatacatacttccgcaaaggaatttttgatgctgtcatgaaaaccatgggcggaaactcttcaggtcaggctatattgagctcgcatgctgtggtaatcgacgcctgcaacaaacttttgaaataatagccccttggcagccttggcatatcaaccttttggatgctgtgcctagccaaaacagcatcgtgtattctctatagcttaggtgtttcaccccagaggaactctcgtactggagatgtgagcaacagccagttttgtgctgtatatttttgcaaggtcagatgagtgttcattattgtttcgacaacgaaggaaagaaagaagccatggattttgctatcgggtgacagcttaattcgctggagcctgagatgaacaaggcttaatgtggtatttttgtgtccctttattagttcttttctgtgcgtacacattttgtaacaatgtactatggttttggagtattctgttggtttacaatatatcgtgtcattgtagcggtcaaattttgatacctaattctgaggtttgct</dnaseqindica> |
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