Difference between revisions of "Os02g0313400"
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===Function=== | ===Function=== | ||
Please input function information here. | Please input function information here. | ||
| − | APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain[[File: | + | APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain[[File:zhao001.jpg]], results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes.A functional transcription activation domain might exist in Os API5 and that the LXXLL motif is a likely suppressor of the transcription activationOs API5 interacts with two DEAD-box ATP-dependent RNA helicases, API5-INTERACTING PROTEIN1 (AIP1) and AIP2 which is directly regulates the expression of CP1. Summing up, the API5 is required for normal expression of CP1 and that AIP1/2 directly regulates the expression of CP1, which might regulate tapetum degeneration during pollen development. |
===mutantion=== | ===mutantion=== | ||
Revision as of 16:29, 22 May 2014
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Contents
Annotated Information
Function
Please input function information here.
APOPTOSIS INHIBITOR5 (API5), which encodes a putative homolog of antiapoptosis protein Api5 in animals,with 537 amino acids, including a nuclear localization signal, a LXXLL motif, and a transcription activation domain
, results in delayed degeneration of the tapetum due to inhibition of the tapetal PCD process leading to defects in formation of male gametophytes.A functional transcription activation domain might exist in Os API5 and that the LXXLL motif is a likely suppressor of the transcription activationOs API5 interacts with two DEAD-box ATP-dependent RNA helicases, API5-INTERACTING PROTEIN1 (AIP1) and AIP2 which is directly regulates the expression of CP1. Summing up, the API5 is required for normal expression of CP1 and that AIP1/2 directly regulates the expression of CP1, which might regulate tapetum degeneration during pollen development.
mutantion
Please input mutantion information here. The api5-1 mutant plants were normal during vegetative and floral development stages but produced smaller anthers (Figures 1A to 1D). The pollen grains of api5-1 lacked starch, as shown by staining with iodine (Figures 1E and 1F).Degeneration of the tapetum is delayed in api5-1(Figures 1G to 1N),but that pollen wall development and orbicule formation are not affected(Figures 2A to 2P).
Expression
Please input expression information here. API5 is expressed in all tissues analyzed, including root, leaf blades, leaf sheath, culms, and panicles (Figure 5A). Unexpectedly, it was expressed more highly in roots than in panicles.Its transcripts were detected in the parietal anther wall layers and microsporocyte, with stronger signal in tapetum in all the examined stages (Figures 5B to 5D). In vegetative tissues, transcripts of API5 were detected in epidermal cells in roots (Figures 5F and 5G), vascular tissues in culms (Figure 5I), and mesophyll cells in leaf blades (Figure 5J). As expected, sense probe used as a negative control gave rise to only a faint, almost undetectable, signal (Figures 5E, 5H, and 5K). The preferential expression of API5 in the tapetum is consistent with its inferred function in the differentiation of the tapetum during anther development. The expression of API5 in vegetative tissues suggests that it has other functions in growth and development that may be complemented by other gene product(s) in the api5 mutant.
Evolution
Please input evolution information here.
Make phylogenetic tree using API5 homologs from protists, plants, and animals. 32 API5 protein sequences were classified into three clusters according to the phylogenetic tree (Figure 6). There are three major groups in the phylogenetic analyses may indeed reflect the systematization of animals, plants, and protists.
Labs working on this gene
Please input related labs here.National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan),Huazhong Agricultural University
References
Please input cited references here.Xingwang Li, Xinqiang Gao, YiWei, Li Deng, Yidan Ouyang, Guoxing Chen, Xianghua Li, Qifa Zhang,and ChangyinWu.Rice APOPTOSIS INHIBITOR5 Coupled with Two DEAD-Box Adenosine 59-Triphosphate-Dependent RNA Helicases Regulates Tapetum Degeneration.The Plant Cell, Vol. 23: 1416–1434, April 2011.
Structured Information
| Gene Name |
Os02g0313400 |
|---|---|
| Description |
Apoptosis inhibitory 5 family protein |
| Version |
NM_001053191.1 GI:115445752 GeneID:4329135 |
| Length |
4125 bp |
| Definition |
Oryza sativa Japonica Group Os02g0313400, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 2:12400052..12404176 |
| Sequence Coding Region |
12400053..12400106,12400553..12400663,12400817..12400903,12400987..12401093,12402041..12402155 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008395:12400052..12404176 source=RiceChromosome02 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008395:12400052..12404176 source=RiceChromosome02 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>agcagcaagttcctcaactatttcgttaagcaaattgttccagtttttgacaagattcctgaagagaagaaactggatttgctcaaaacagttgctgcaagctcaccatatgctacagcacaagatgcacggcagctgcttccccctgttgttcaattgctcaagaaatatatgcctgggaagaaggtggaagatatcaaccataattatgttgagtgcctgatgtacatttttcaccatttagcccataagacaccaaacaccacaaacagcctatgtggttacaagattgttactggacaaccatcggatagacttggagaggatttctcagaacattacaaagactttacggagaggttaactggaacagaagagacagttcgagcagtctcaaagagactaactcaggggatggcagattttaacaaggcaatatcttcagcaaagactgaagaagagaaaactaaaattaaatctgatcagcagaaatcaacaatgacaatgagggcctacaacaacatactagcaatggcgcagccgttgcgtgcgaaatcccctttatttattggtgacaagaaaatcactctctcatggatggagcagccaaagaaaccagcacctacaacaacaggagggaagaggtcccagcctgctacaaatgggaacacccctgcgagcaagaaggggcggggagagggagcagcccggaatcaacttgtgaacagagcttttgaagggttatcccgtggtggtagagggagtggaagaggccggggcaggggaggccggggaagaggatggggctaccgctga</cdnaseq> |
| Protein Sequence |
<aaseq>SSKFLNYFVKQIVPVFDKIPEEKKLDLLKTVAASSPYATAQDAR QLLPPVVQLLKKYMPGKKVEDINHNYVECLMYIFHHLAHKTPNTTNSLCGYKIVTGQP SDRLGEDFSEHYKDFTERLTGTEETVRAVSKRLTQGMADFNKAISSAKTEEEKTKIKS DQQKSTMTMRAYNNILAMAQPLRAKSPLFIGDKKITLSWMEQPKKPAPTTTGGKRSQP ATNGNTPASKKGRGEGAARNQLVNRAFEGLSRGGRGSGRGRGRGGRGRGWGYR</aaseq> |
| Gene Sequence |
<dnaseqindica>2..55#502..612#766..852#936..1042#1990..2104#2321..2386#3370..3464#3549..3729#gagcagcaagttcctcaactatttcgttaagcaaattgttccagtttttgacaaggtaatcttctttatcttgtacattggtccctgtgctttgttggaaaaaacagtaaatgcttctttttaattatattatgcatggcagctctacaacccttgttttgttgtcatacataatgtaacattttgcagtttgcacaccattttttttttgaaacatttggttacattccttacttgtgataaagaaacagctcttgcaatcatgcacatttctgcattctgcactaacattgcatggtaaaaaaaagtctgtcgtatttttttgtaactatggatattctaattgaattgccatcattttggcaatcagactccccaccatgccttgaacttgtttatcttcaaaacaaagtgaattcgcatcttagagcatgaaggttttctagagcatgaaggttttctagagcattaaggttttcccctcttttttacctgttttagattcctgaagagaagaaactggatttgctcaaaacagttgctgcaagctcaccatatgctacagcacaagatgcacggcagctgcttccccctgttgttcaattgctcaaggtctgtgcaattcctgtctgataggtgtccaatccccctttgttaatgttttattttgtcccctagaagacattgttagatggtttcccctccaccgcggtgttacacaataaatgtcgtgtcttaccattgcatttacgttttcttttacagaaatatatgcctgggaagaaggtggaagatatcaaccataattatgttgagtgcctgatgtacatttttcaccatttagcccataaggtttgatgacagaatgtttatcatctattatgaaccatgtcttttcttcatggatggccttgtgctgaccaaatgaaatacagacaccaaacaccacaaacagcctatgtggttacaagattgttactggacaaccatcggatagacttggagaggatttctcagaacattacaaagactttacggagaggtaggttgcattttccttatgatttctttgaactatttttatttgtgtgcattcctttaagaaccacagcttagacaatggttattttttaaattagttccaccaaatcattagttatcatacattggggcagttgcagtagtaccatgactaccacgtgcttaagtggacaaaaaaaatctagcactagggtgaaggaatccatcttgtctgagtatgatcctgaggtaaggggtaattgacatgtaaaaaatgagtgatgccatgacgctagtatctgggtcacatctattgttaaatcttagaataaaataataggttgtactagaagatttgaccaaaggtacattgtaaatggatgatctgtagtaattttatataaatatggacatatagataattgaagaataattggaatttggaagtgatttgcatgattgttaaggcatcgctgtagtgctagggtgtgccaaggggtcagggcgtaagggcttaacgccataacccagagagagagagagagagatgtgaggtttccactctcttcttccccctgtgccactatcaagattctacctaggattgaatcagatcaattattctatggttgttcgactagtagacttgtattaagattttaacaaacatgatgcttggcttggcggtttccgttgatgaggaaaatagacaatttgttataactctcttaattggccgtcattcgctatgtgtggcaacattatcttacacaaattttgttctcccatagcttgcgaaacatcttttacgccatattctggtgtggtaatctaatataaatcataaatcgtttttctataaattatgataaaattcctttcaattttttaatatggtgtgagagcgtatggtattttgttttggtgatctgttttatttgctagtttcttactgctgcacgtgcacaggttaactggaacagaagagacagttcgagcagtctcaaagagactaactcaggggatggcagattttaacaaggcaatatcttcagcaaagactgaagaagagaaaactaaaattgtgagtttttaggtgctttgcataaattttctatgttcctccttccgtgttggatcagcactattgttagctgacttatccttgtctcagaatcttgtaacatcatattctacaagattgtttccaagttctttgtttggcaattagattgatactatgactttaaaaacaattacaacaactgatcatcgtttggatatgatgttcttttggcagaaatctgatcagcagaaatcaacaatgacaatgagggcctacaacaacatactagcaatggcgcaggtgatttctttattactttagtggaactgtgtgatatgtgaattgcaaaatgctatcgcatgtgagaataatgtggtgacacatatgagggtatttttgaaatcacaagaagcttccaaatgctctctgcttgggcttctcatggtggaccagttacaattttaaatgtataacaaaagaactattgaaaatactagcaaatagcagtgggagtttttttttcgttacagatctgcttttgagcatagtaccgaaatctgaccactgttagtcaagaccaaaaaaaaaactgtgaccagggcccggtctggtttggattaatagaaacaatgatagtgagaaaaactgggatgtttaaactttctggtacattttaattccgcttggagtcgaatcccttttacctgaggttatattgcatgtgtaggatgtgctgataagcaactgtgttgagattggcatgtgatttatttaaggaagttttcaatatgagcctttattttaatacaggcttggggtttaattcaaaattaatactctgaactgtctggctcactgtcaaaccatgtttgaatcagcgaaccgaggactgatttgagtgaatagttcacatgctggtttgagttttgcactatgcttttgaggcctgtgaaggaactgcaggactatcttaagtaaaagttaagtggccttttataattgcatgtacacaaccgaattcaactttggctaccaatctctacgaattaccttagatatatgaaaatgacgagtaggttgtgtcaaaaaattccattgtagttgtattaatttgtacaatggaaaagttgcatgtgttaatgtctaaaacacatcttattctactgctggaggaagcataagtacacggcaatgtatgaaatatactgcttgtgtatactaataatactaacctacctaaatgtttatgctttctaactaaacgtgatgaatgttcatttcagccgttgcgtgcgaaatcccctttatttattggtgacaagaaaatcactctctcatggatggagcagccaaagaaaccagcacctacaacaacagggttagatttctattacctaatattccatttccccctcagactttgcatttcattatagttaagtctgctttgtggatcttttagagggaagaggtcccagcctgctacaaatgggaacacccctgcgagcaagaaggggcggggagagggagcagcccggaatcaacttgtgaacagagcttttgaagggttatcccgtggtggtagagggagtggaagaggccggggcaggggaggccggggaagaggatggggctaccgctgagaatagttggaaatccctgcaatctgatgggagaatagatcagctttctgctttgatttgaggtacttctcaggagataagcttttctgtatgcagcaaaatgggattggtgatagcccgtgaaggcatgctcaattgctgaatgcatacagaaagctcatatgatggatgcttgagtatatcgatgattttttagaccctagtcgtggaggttcaagaatgtggagtgtatgtacgccctagttcatttacatccctgaccgtttgttagttgaccagttctacttgttttggtggttcgatgaactcaagcagaacttttaaattctagaatttgttgttaaagactatttatttaagaatcaatagattgccgaggtgaattatgtttattgc</dnaseqindica> |
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