Difference between revisions of "Os06g0133000"
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Please input evolution information here. | Please input evolution information here. | ||
| − | + | Waxy mutants of cereals have a high content of amylopectin and have been well characterized. However, the allocation of carbon to other components, such as beta-glucan and oils, and the regulation of the altered carbon distribution to amylopectin in a waxy mutant are poorly understood. In this study, we used a rice mutant, GM077, with a low content of amylose to gain molecular insight into how a deficiency of amylose affects carbon allocation to other end products and to amylopectin. We used carbohydrate analysis, subtractive cDNA libraries, and qPCR to identify candidate genes potentially responsible for the changes in carbon allocation in GM077 seeds | |
==Labs working on this gene== | ==Labs working on this gene== | ||
Revision as of 16:18, 23 May 2014
Please input one-sentence summary here.
Contents
Annotated Information
Function
Please input function information here. Carbohydrate analysis indicated that the content of amylose in GM077 seeds was significantly reduced, while that of amylopectin significantly rose as compared to the wild type BP034. The content of glucose, sucrose, total starch, cell-wall polysaccharides and oil were only slightly affected in the mutant as compared to the wild type. Suppression subtractive hybridization (SSH) experiments generated 116 unigenes in the mutant on the wild-type background. Among the 116 unigenes, three, AGP, ISA1 and SUSIBA2-like, were found to be directly involved in amylopectin synthesis, indicating their possible roles in redirecting carbon flux from amylose to amylopectin. A bioinformatics analysis of the putative SUSIBA2-like binding elements in the promoter regions of the upregulated genes indicated that the SUSIBA2-like transcription factor may be instrumental in promoting the carbon reallocation from amylose to amylopectin.
Expression
Please input expression information here. Analyses of carbohydrate and oil fractions and gene expression profiling on a global scale in the rice waxy mutant GM077 revealed several candidate genes implicated in the carbon reallocation response to an amylose deficiency, including genes encoding AGPase and SUSIBA2-like. We believe that AGP and SUSIBA2 are two promising targets for classical breeding and/or transgenic plant improvement to control the carbon flux between starch and other components in cereal seeds
Evolution
Please input evolution information here.
Waxy mutants of cereals have a high content of amylopectin and have been well characterized. However, the allocation of carbon to other components, such as beta-glucan and oils, and the regulation of the altered carbon distribution to amylopectin in a waxy mutant are poorly understood. In this study, we used a rice mutant, GM077, with a low content of amylose to gain molecular insight into how a deficiency of amylose affects carbon allocation to other end products and to amylopectin. We used carbohydrate analysis, subtractive cDNA libraries, and qPCR to identify candidate genes potentially responsible for the changes in carbon allocation in GM077 seeds
Labs working on this gene
Please input related labs here.
References
Please input cited references here. 1.Ming-Zhou Zhang; Jie-Hong Fang; Xia Yan; Jun Liu; Jin-Song Bao; Gunnel Fransson; Roger Andersson; Christer Jansson; Per Å man; Chuanxin Sun. Molecular insights into how a deficiency of amylose affects carbon allocation -- carbohydrate and oil analyses and gene expression profiling in the seeds of a rice waxy mutant BMC Plant Biology, 2012, 12: 230.
Structured Information
| Gene Name |
Os06g0133000 |
|---|---|
| Description |
Granule-bound starch synthase I, chloroplast precursor (EC 2.4.1.21) |
| Version |
NM_001063239.1 GI:115466209 GeneID:4340018 |
| Length |
4953 bp |
| Definition |
Oryza sativa Japonica Group Os06g0133000, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 6:1764623..1769575 |
| Sequence Coding Region |
1765922..1766260,1766374..1766454,1766562..1766660,1766757..1766846,1766946..1767009 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008399:1764623..1769575 source=RiceChromosome06 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008399:1764623..1769575 source=RiceChromosome06 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atgtcggctctcaccacgtcccagctcgccacctcggccaccggcttcggcatcgccgacaggtcggcgccgtcgtcgctgctccgccacgggttccagggcctcaagccccgcagccccgccggcggcgacgcgacgtcgctcagcgtgacgaccagcgcgcgcgcgacgcccaagcagcagcggtcggtgcagcgtggcagccggaggttcccctccgtcgtcgtgtacgccaccggcgccggcatgaacgtcgtgttcgtcggcgccgagatggccccctggagcaagaccggcggcctcggtgacgtcctcggtggcctcccccctgccatggctgcgaatggccacagggtcatggtgatctctcctcggtacgaccagtacaaggacgcttgggataccagcgttgtggctgagatcaaggttgcagacaggtacgagagggtgaggtttttccattgctacaagcgtggagtcgaccgtgtgttcatcgaccatccgtcattcctggagaaggtttggggaaagaccggtgagaagatctacggacctgacactggagttgattacaaagacaaccagatgcgtttcagccttctttgccaggcagcactcgaggctcctaggatcctaaacctcaacaacaacccatacttcaaaggaacttatggtgaggatgttgtgttcgtctgcaacgactggcacactggcccactggcgagctacctgaagaacaactaccagcccaatggcatctacaggaatgcaaaggttgctttctgcatccacaacatctcctaccagggccgtttcgctttcgaggattaccctgagctgaacctctccgagaggttcaggtcatccttcgatttcatcgacgggtatgacacgccggtggagggcaggaagatcaactggatgaaggccggaatcctggaagccgacagggtgctcaccgtgagcccgtactacgccgaggagctcatctccggcatcgccaggggatgcgagctcgacaacatcatgcggctcaccggcatcaccggcatcgtcaacggcatggacgtcagcgagtgggatcctagcaaggacaagtacatcaccgccaagtacgacgcaaccacggcaatcgaggcgaaggcgctgaacaaggaggcgttgcaggcggaggcgggtcttccggtcgacaggaaaatcccactgatcgcgttcatcggcaggctggaggaacagaagggccctgacgtcatggccgccgccatcccggagctcatgcaggaggacgtccagatcgttcttctgggtactggaaagaagaagttcgagaagctgctcaagagcatggaggagaagtatccgggcaaggtgagggccgtggtgaagttcaacgcgccgcttgctcatctcatcatggccggagccgacgtgctcgccgtccccagccgcttcgagccctgtggactcatccagctgcaggggatgagatacggaacgccctgtgcttgcgcgtccaccggtgggctcgtggacacggtcatcgaaggcaagactggtttccacatgggccgtctcagcgtcgactgcaaggtggtggagccaagcgacgtgaagaaggtggcggccaccctgaagcgcgccatcaaggtcgtcggcacgccggcgtacgaggagatggtcaggaactgcatgaaccaggacctctcctggaaggggcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgccggcagcgcgccggggatcgaaggcgacgagatcgcgccgctcgccaaggagaacgtggctgctccttga</cdnaseq> |
| Protein Sequence |
<aaseq>MSALTTSQLATSATGFGIADRSAPSSLLRHGFQGLKPRSPAGGD ATSLSVTTSARATPKQQRSVQRGSRRFPSVVVYATGAGMNVVFVGAEMAPWSKTGGLG DVLGGLPPAMAANGHRVMVISPRYDQYKDAWDTSVVAEIKVADRYERVRFFHCYKRGV DRVFIDHPSFLEKVWGKTGEKIYGPDTGVDYKDNQMRFSLLCQAALEAPRILNLNNNP YFKGTYGEDVVFVCNDWHTGPLASYLKNNYQPNGIYRNAKVAFCIHNISYQGRFAFED YPELNLSERFRSSFDFIDGYDTPVEGRKINWMKAGILEADRVLTVSPYYAEELISGIA RGCELDNIMRLTGITGIVNGMDVSEWDPSKDKYITAKYDATTAIEAKALNKEALQAEA GLPVDRKIPLIAFIGRLEEQKGPDVMAAAIPELMQEDVQIVLLGTGKKKFEKLLKSME EKYPGKVRAVVKFNAPLAHLIMAGADVLAVPSRFEPCGLIQLQGMRYGTPCACASTGG LVDTVIEGKTGFHMGRLSVDCKVVEPSDVKKVAATLKRAIKVVGTPAYEEMVRNCMNQ DLSWKGPAKNWENVLLGLGVAGSAPGIEGDEIAPLAKENVAAP</aaseq> |
| Gene Sequence |
<dnaseqindica>1300..1638#1752..1832#1940..2038#2135..2224#2324..2387#2480..2580#2671..2780#2902..3145#3263..3439#3683..3874#3981..4067#4177..4305#4663..4779#accattccttcagttctttgtctatctcaagacacaaataactgcagtctctctctctctctctctctctctctctctctctctgcttcacttctctgcttgtgttgttctgttgttcatcaggaagaacatctgcaagttatacatatatgtttataattctttgtttcccctcttattcagatcgatcacatgcatctttcattgctcgtttttccttacaagtagtctcatacatgctaatttctgtaaggtgttgggctggaaattaattaattaattaattgacttgccaagatccatatatatgtcctgatattaaatcttcgttcgttatgtttggttaggctgatcaatgttattctagagtctagagaaacacacccaggggttttccaactagctccacaagatggtgggctagctgacctagatttgaagtctcactccttataattattttatattagatcattttctaatattcgtgtctttttttattctagagtctagatcttgtgttcaactctcgttaaatcatgtctctcgccactggagaaacagatcaggagggtttattttgggtataggtcaaagctaagattgaaattcacaaatagtaaaatcagaatccaaccaattttagtagccgagttggtcaaaggaaaatgtatatagctagatttattgttttggcaaaaaaaaatctgaatatgcaaaatacttgtatatctttgtattaagaagatgaaaataagtagcagaaaattaaaaaatggattatatttcctgggctaaaagaattgttgatttggcacaattaaattcagtgtcaaggttttgtgcaagaattcagtgtgaaggaatagattctcttcaaaacaatttaatcattcatctgatctgctcaaagctctgtgcatctccgggtgcaacggccaggatatttattgtgcagtaaaaaaatgtcatatcccctagccacccaagaaactgctccttaagtccttataagcacatatggcattgtaatatatatgtttgagttttagcgacaatttttttaaaaacttttggtcctttttatgaacgttttaagtttcactgtctttttttttcgaattttaaatgtagcttcaaattctaatccccaatccaaattgtaataaacttcaattctcctaattaacatcttaattcatttatttgaaaaccagttcaaattcttttaggctcaccaaaccttaaacaattcaattcagtgcagagatcttccacagcaacagctagacaaccaccatgtcggctctcaccacgtcccagctcgccacctcggccaccggcttcggcatcgccgacaggtcggcgccgtcgtcgctgctccgccacgggttccagggcctcaagccccgcagccccgccggcggcgacgcgacgtcgctcagcgtgacgaccagcgcgcgcgcgacgcccaagcagcagcggtcggtgcagcgtggcagccggaggttcccctccgtcgtcgtgtacgccaccggcgccggcatgaacgtcgtgttcgtcggcgccgagatggccccctggagcaagaccggcggcctcggtgacgtcctcggtggcctcccccctgccatggctgtaagcacacacaaacttcgatcgctcgtcgtcgctgaccgtcgtcgtcttcaactgttcttgatcatcgcattggatggatgtgtaatgttgtgttcttgtgttctttgcaggcgaatggccacagggtcatggtgatctctcctcggtacgaccagtacaaggacgcttgggataccagcgttgtggctgaggtaggagcatatgcgtgatcagatcatcacaagatcgattagctttagatgatttgttacatttcgcaagattttaacccaagtttttgtggtgcaattcattgcagatcaaggttgcagacaggtacgagagggtgaggtttttccattgctacaagcgtggagtcgaccgtgtgttcatcgaccatccgtcattcctggagaaggtggagtcatcattagtttaccttttttgtttttactgaattattaacagtgcatttagcagttggactgagcttagcttccactggtgatttcaggtttggggaaagaccggtgagaagatctacggacctgacactggagttgattacaaagacaaccagatgcgtttcagccttctttgccaggtcagtgattacttctatctgatgatggttggaagcatcacgagtttaccatagtatgtatggattcataactaattcgtgtattgatgctacctgcaggcagcactcgaggctcctaggatcctaaacctcaacaacaacccatacttcaaaggaacttatggtgagttacaattgatctcaagatcttataactttcttcgaaggaatccatgatgatcagactaattccttccggtttgttactgacaacaggtgaggatgttgtgttcgtctgcaacgactggcacactggcccactggcgagctacctgaagaacaactaccagcccaatggcatctacaggaatgcaaaggtctatgcttgttcttgccataccaactcaaatctgcatgcacactgcattctgttcagaaactgactgtctgaatctttttcactgcaggttgctttctgcatccacaacatctcctaccagggccgtttcgctttcgaggattaccctgagctgaacctctccgagaggttcaggtcatccttcgatttcatcgacgggtatgagtaagattctaagagtaacttactgtcaattcgccatatatcgattcaatccaagatccttttgagctgacaaccctgcactactgtccatcgttcaaatccggttaaatttcaggtatgacacgccggtggagggcaggaagatcaactggatgaaggccggaatcctggaagccgacagggtgctcaccgtgagcccgtactacgccgaggagctcatctccggcatcgccaggggatgcgagctcgacaacatcatgcggctcaccggcatcaccggcatcgtcaacggcatggacgtcagcgagtgggatcctagcaaggacaagtacatcaccgccaagtacgacgcaaccacggtaagaacgaatgcattcttcacaagatatgcaatctgaattttctttgaaaaagaaattatcatctgtcacttcttgattgattctgacaaggcaagaatgagtgacaaatttcaggcaatcgaggcgaaggcgctgaacaaggaggcgttgcaggcggaggcgggtcttccggtcgacaggaaaatcccactgatcgcgttcatcggcaggctggaggaacagaagggccctgacgtcatggccgccgccatcccggagctcatgcaggaggacgtccagatcgttcttctggtataatataatacactacaagacacacttgcacgatatgccaaaaattcagaacaaattcagtggcaaaaaaaaaactcgaatattagggaaggacctaataatatcaaataattagaaggggtgaggctttgaacccagatcgtctagtccaccaccttgtggagttagccggaagacctctgagcatttctcaattcagtggcaaatgatgtgtataattttgatccgtgtgtgtttcagggtactggaaagaagaagttcgagaagctgctcaagagcatggaggagaagtatccgggcaaggtgagggccgtggtgaagttcaacgcgccgcttgctcatctcatcatggccggagccgacgtgctcgccgtccccagccgcttcgagccctgtggactcatccagctgcaggggatgagatacggaacggtatacaatttccatctatcaattcgattgttcgatttcatctttgtgcaatgcaatgcaattgcaaatgcaaatgcatgatgattttccttgttgatttctccagccctgtgcttgcgcgtccaccggtgggctcgtggacacggtcatcgaaggcaagactggtttccacatgggccgtctcagcgtcgacgtaagcctatacatttacataacaatcagatatgacacatcctaataccgataagtcggtacactactacacatttacatggttgctggttatatggtttttttggcagtgcaaggtggtggagccaagcgacgtgaagaaggtggcggccaccctgaagcgcgccatcaaggtcgtcggcacgccggcgtacgaggagatggtcaggaactgcatgaaccaggacctctcctggaaggtataaattacgaaacaaatttaacccaaacatatactatatactccctccgcttctaaatattcaacgccgttgtcttttttaaatatgtttgaccattcgtcttattaaaaaaattaaataattataaattcttttcctatcatttgattcattgttaaatatacttatatgtatacatatagttttacatatttcataaaattttttgaacaagacgaacggtcaaacatgtgctaaaaagttaacggtgtcgaatattcagaaacggagggagtataaacgtcttgttcagaagttcagagattcacctgtctgatgctgatgatgattaattgtttgcaacatggatttcaggggcctgcgaagaactgggagaatgtgctcctgggcctgggcgtcgccggcagcgcgccggggatcgaaggcgacgagatcgcgccgctcgccaaggagaacgtggctgctccttgaagagcctgagatctacatatggagtgattaattaatatagcagtatatggatgagagacgaatgaaccagtggtttgtttgttgtagtgaatttgtagctatagccaattatataggctaataagtttgatgttgtactcttctgggtgtgcttaagtatcttatcggaccctg</dnaseqindica> 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