Difference between revisions of "Os05g0482400"
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| − | + | The rice EUI1 (ELONGATED UPPERMOST INTERNODE) gene controls the growth of the first internode at the top of rice, and the recessive mutations of this site cause the abnormal elongation of internodes at the top of rice. | |
==Annotated Information== | ==Annotated Information== | ||
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The EUI1 gene was mapped on the central part of the long arm of chromosome 5, and located within two overlapping bacterial artificial chromosome (BAC) clones, OSJNBa0018K15 and OSJNBa0095J22, in an interval of 98 kb between markers M0387 and M01 <ref name="ref2" />.The EUI1 gene encodes a putative cytochrome P450 protein, CYP714D1. It can control the elongation of internodes and determines the plant height and underlies the grain yield .EUI1 plays a negative role in gibberellinmediated regulation of cell elongation in the uppermost internode of rice <ref name="ref3" /><ref name="ref4" />. | The EUI1 gene was mapped on the central part of the long arm of chromosome 5, and located within two overlapping bacterial artificial chromosome (BAC) clones, OSJNBa0018K15 and OSJNBa0095J22, in an interval of 98 kb between markers M0387 and M01 <ref name="ref2" />.The EUI1 gene encodes a putative cytochrome P450 protein, CYP714D1. It can control the elongation of internodes and determines the plant height and underlies the grain yield .EUI1 plays a negative role in gibberellinmediated regulation of cell elongation in the uppermost internode of rice <ref name="ref3" /><ref name="ref4" />. | ||
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| + | EUI catalyzed 16a,17-epoxidation of non-13-hydroxylated GAs. Consistent with the tall and dwarfed phenotypes of the eui mutant and Eui-overexpressing transgenic plants,respectively,16a,17-epoxidation reduced the biological activity of GA4 in rice,demonstrating that EUI functions as a GA deactivating enzyme<ref name="ref6" />. | ||
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| + | Transgenic RNA interference of the Eui gene effectively increased plant height and improved heading performance. Furthermore, the eui mutant was defective in starch granule development in root caps and Eui overexpression enhanced starch granule generation and gravity responses, revealing a role for Eui in root starch granule development and gravity responses<ref name="ref5" />. | ||
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===Expression=== | ===Expression=== | ||
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<ref name="ref4">Ma Hongli;Zhang Shubiao;Ji Lan;Zhu Hongbo;Yang Shulan;Fang Xuanjun;Yang Rencui. Fine Mapping and in silico Isolation of the EUI1 Gene Controlling Upper Internode Elongation in Rice.Plant Molecular Biology, 2006, 60(1): 87-94.</ref> | <ref name="ref4">Ma Hongli;Zhang Shubiao;Ji Lan;Zhu Hongbo;Yang Shulan;Fang Xuanjun;Yang Rencui. Fine Mapping and in silico Isolation of the EUI1 Gene Controlling Upper Internode Elongation in Rice.Plant Molecular Biology, 2006, 60(1): 87-94.</ref> | ||
<ref name="ref5">Yingying Zhang;Yongyou Zhu;Yu Peng;Dawei Yan;Qun Li;Jianjun Wang;Linyou Wang;Zuhua He.Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice.Cell Research, 2008, 18(3): 412-421.</ref> | <ref name="ref5">Yingying Zhang;Yongyou Zhu;Yu Peng;Dawei Yan;Qun Li;Jianjun Wang;Linyou Wang;Zuhua He.Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice.Cell Research, 2008, 18(3): 412-421.</ref> | ||
| + | <ref name="ref6"> Yongyou Zhu;Takahito Nomura;Yonghan Xu;Yingying Zhang;Yu Peng;Bizeng Mao;Atsushi Hanada;Haicheng Zhou;Renxiao Wang;Peijin Li;Xudong Zhu;Lewis N. Mander;Yuji Kamiya;Shinjiro Yamaguchi;Zuhua He.''ELONGATED UPPERMOST INTERNODE''''Italic text'' Encodes a Cytochrome P450 Monooxygenase That Epoxidizes Gibberellins in a Novel Deactivation Reaction in Rice.The Plant Cell, 2006, 18(2): 442-456.</ref> | ||
</references> | </references> | ||
Revision as of 05:05, 8 June 2014
The rice EUI1 (ELONGATED UPPERMOST INTERNODE) gene controls the growth of the first internode at the top of rice, and the recessive mutations of this site cause the abnormal elongation of internodes at the top of rice.
Contents
Annotated Information
Function
EUI gene is a recessive gene which was first reported by Rutger and Carnahan [1], which is characterized by the near doubling length of the uppermost internode, excess panicle exsertion and an increased panicle length in comparison with the wild type.The EUI mutant shows notably rapid elongation of the uppermost internode at the heading stage. Because of its prospective application to amend panicle enclosure in the male-sterile lines, this recessive trait, along with male sterility,maintainer and restorer, was referred to as the fourth genetic element of hybrid rice production [1].
The EUI1 gene was mapped on the central part of the long arm of chromosome 5, and located within two overlapping bacterial artificial chromosome (BAC) clones, OSJNBa0018K15 and OSJNBa0095J22, in an interval of 98 kb between markers M0387 and M01 [2].The EUI1 gene encodes a putative cytochrome P450 protein, CYP714D1. It can control the elongation of internodes and determines the plant height and underlies the grain yield .EUI1 plays a negative role in gibberellinmediated regulation of cell elongation in the uppermost internode of rice [3][4].
EUI catalyzed 16a,17-epoxidation of non-13-hydroxylated GAs. Consistent with the tall and dwarfed phenotypes of the eui mutant and Eui-overexpressing transgenic plants,respectively,16a,17-epoxidation reduced the biological activity of GA4 in rice,demonstrating that EUI functions as a GA deactivating enzyme[5].
Transgenic RNA interference of the Eui gene effectively increased plant height and improved heading performance. Furthermore, the eui mutant was defective in starch granule development in root caps and Eui overexpression enhanced starch granule generation and gravity responses, revealing a role for Eui in root starch granule development and gravity responses[6].
Expression
Luo et al.[3] found that EUI1 is expressed in most tested tissues and organs, including the young leaves, young roots, shoot apical meristem, the first and the second elongating internodes, flag leaf and young panicles. EUI1 is expressed at a relatively low level in the first elongating internodes, and the highest expression level was found in the young panicles during heading.
Overexpression of EUI1 gave rise to the gibberellin-deficient-like phenotypes, which could be partially reversed by supplementation with gibberellin.Furthermore, apart from the alteration of expression levels of the gibberellin biosynthesis genes, accumulation of SLR1 protein was found in the overexpressing transgenic plants,indicating that the expression level of EUI1 is implicated in both gibberellin-mediated SLR1 destruction and a feedback regulation in gibberellin biosynthesis.The rice EUI gene encodes a cytochrome P450 monooxygenase that deactivates bioactive gibberellins (GAs). A slight increase in EUI expression could dramatically change rice morphology,indicating the practical application of the EUI gene in rice molecular breeding for a high yield potential [6].
Evolution
The EUI1 gene encodes a putative cytochrome P450 protein. Cytochrome P450 proteins have been known as hemebinding enzymes with mono-oxygenase activities. Some members of the plant P450 family are known to be involved in biosynthesis of plant growth regulators such as gibberellins,jasmonic acid, auxin and brassinosteroids.The phylogenetic analysis of rice and Arabidopsis cytochrome P450 proteins shows that the EUI1/CYP714D1 protein belongs to the CYP714 subfamily of the CYP72 group. The CYP72 clan seems primarily to have conserved functions involved in plant hormone homeostasis. Three known proteins, CYP735A1, CYP735A2 and CYP72A1, belonging to the CYP72 group exhibit the greatest similarity to the EUI1 protein. CYP735A1 and CYP735A2 have been identified as cytokinin biosynthetic enzymes, and CYP72A1 is a secologanin synthase. According to the sequence similarity and the phylogenetic relationship between EUI1 and the three proteins, the EUI1 gene possibly encodes a putative cytochrome P450 involved in gibberellin homeostasis [3].
Labs working on this gene
Please input related labs here.
References
- ↑ 1.0 1.1 Rutger, J.N. and Carnahan, H.L. (1981) A fourth genetic element to facilitate hybrid cereal production. A recessive tall in rice. Crop Sci. 21: 373–376.
- ↑ Xu, Y.H., Zhu, Y.Y., Zhou, H.C., Li, Q., Sun, Z.X., Liu, Y.G., Lin, H.X. and He,Z.H. (2004) Identification of a 98-kb DNA segment containing the rice EUI gene controlling uppermost internode elongation, and construction of a TAC transgene sublibrary. Mol. Gen. Genet. 272: 149–155.
- ↑ 3.0 3.1 3.2 Anding Luo;Qian Qian;Hengfu Yin;Xiaoqiang Liu;Changxi Yin;Ying Lan;Jiuyou Tang;Zuoshun Tang;Shouyun Cao;Xiujie Wang;Kai Xia;Xiangdong Fu;Da Luo;and Chengcai Chu. EUI1, Encoding a Putative Cytochrome P450 Monooxygenase, Regulates the Internodes Elongation by Modulating GA Responses in Rice.Plant and Cell Physiology, 2006, 47(2): 181-191.
- ↑ Ma Hongli;Zhang Shubiao;Ji Lan;Zhu Hongbo;Yang Shulan;Fang Xuanjun;Yang Rencui. Fine Mapping and in silico Isolation of the EUI1 Gene Controlling Upper Internode Elongation in Rice.Plant Molecular Biology, 2006, 60(1): 87-94.
- ↑ Yongyou Zhu;Takahito Nomura;Yonghan Xu;Yingying Zhang;Yu Peng;Bizeng Mao;Atsushi Hanada;Haicheng Zhou;Renxiao Wang;Peijin Li;Xudong Zhu;Lewis N. Mander;Yuji Kamiya;Shinjiro Yamaguchi;Zuhua He.ELONGATED UPPERMOST INTERNODE'Italic text Encodes a Cytochrome P450 Monooxygenase That Epoxidizes Gibberellins in a Novel Deactivation Reaction in Rice.The Plant Cell, 2006, 18(2): 442-456.
- ↑ 6.0 6.1 Yingying Zhang;Yongyou Zhu;Yu Peng;Dawei Yan;Qun Li;Jianjun Wang;Linyou Wang;Zuhua He.Gibberellin homeostasis and plant height control by EUI and a role for gibberellin in root gravity responses in rice.Cell Research, 2008, 18(3): 412-421.
Structured Information
| Gene Name |
Os05g0482400 |
|---|---|
| Description |
Cytochrome P450 family protein |
| Version |
NM_001062401.1 GI:115464532 GeneID:4339131 |
| Length |
9805 bp |
| Definition |
Oryza sativa Japonica Group Os05g0482400, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 5:23775727..23785531 |
| Sequence Coding Region |
23775837..23776191,23784066..23785444 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008398:23775727..23785531 source=RiceChromosome05 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008398:23775727..23785531 source=RiceChromosome05 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggagagcttcttcgtcttcttcacggcggcggcgttgccggtggtggtggcggcggcggtgatcgccgggctgtgcattacggcggcgtggctggcgaggccgcggcgcgtggcggaggtgttccggaggcaggggatcgacggcccgccgccgtcgtcgttcctggcggggaacctcccggagatgaaggcgagggtggccgccgcggcgtcggcggcggcgccaacggcggacggggaggagaccgcctccgccggcggcggcggcggtggccgggacttcgagaaggacgggttcgacgactactgcaccaggatcttcccttacttccacaagtggaggaaagcctacggcgagacgtacctgtactggctgcggcggcggccggcgctgtacgtgacggacccggagctcatcggcgagatcgggcggtgcgtgtcgctcgacatgggcaagcccaagtacctccagaaaggccaggagccactcttcggcggcggcgtcctcaaggccaacggcgcgtgctgggcgcgccagcgcaaggtcatcgcgccggagttctacatggcccgtgtcagggccatggtccagctcatggtcgacgccgcgcagccgctgatcgcctcctgggaatccaggatcgacgccgctggaggcgcggcggcggcggaggtcgtcgtcgacggcgacctccggagcttctccttcgatgtgatatcgcgggcttgctttgggagtgattactcgagggggagggagatcttcctccgcctccgtgagctgtccgggctcatgtcggagaccagcgtcatcttcagcatcccttcgctgaggcacctgccgacggggaagaaccggaggatctggaggctcacgggggagatccggtcgctgatcatggagctcgtcagggagcggaggtgcgcggcgagggcggcgagggagcacggcgggaaggcggcgccgccgtcgccgccggagcgcgacttcctcggctccatcatcgagaacagcggcgggcagccgcggccggacgacttcgtggtggacaactgcaagaacatctacttcgccgggcacgagacgagcgcggtcaccgcgacgtggtgcctcatgctgctcgccgcgcacccggagtggcaggaccgcgcccgcgccgaggtgctcgaggtctgcggcggcgacggcgccgccgcccccgccgcgccggacttcgacatggtgtcccggatgcggacggtggggatggtggtgcaggaaacgctgcggctgttcccgccgtcgtcgttcgtggtgcgggagacgttccgggacatgcagttgggtaggctgctggcgcccaagggcacctacctgttcgtcccggtgtccaccatgcaccacgacgtcgccgcctggggcccgacggcgaggctgttcgacccgtcccgcttccgcgacggcgtggcggcggcgtgcaagcacccgcaggcgtcgttcatgccgttcggcctcggcgcccgcacctgcctcggccagaacctcgcgctcgtcgaggtcaagacgctcgtcgccgtcgtcctcgcccggttcgagttcacgctctcgccggagtacaggcactcgccggcgttccggctcatcatcgagccggagttcggcctccgcctccgcatccgccgcgccggcggtcaggacgccacgtcacaagttgacacatctactgcacccgtgcatagttctcataattaa</cdnaseq> |
| Protein Sequence |
<aaseq>MESFFVFFTAAALPVVVAAAVIAGLCITAAWLARPRRVAEVFRR QGIDGPPPSSFLAGNLPEMKARVAAAASAAAPTADGEETASAGGGGGGRDFEKDGFDD YCTRIFPYFHKWRKAYGETYLYWLRRRPALYVTDPELIGEIGRCVSLDMGKPKYLQKG QEPLFGGGVLKANGACWARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAA GGAAAAEVVVDGDLRSFSFDVISRACFGSDYSRGREIFLRLRELSGLMSETSVIFSIP SLRHLPTGKNRRIWRLTGEIRSLIMELVRERRCAARAAREHGGKAAPPSPPERDFLGS IIENSGGQPRPDDFVVDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEV CGGDGAAAPAAPDFDMVSRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKG TYLFVPVSTMHHDVAAWGPTARLFDPSRFRDGVAAACKHPQASFMPFGLGARTCLGQN LALVEVKTLVAVVLARFEFTLSPEYRHSPAFRLIIEPEFGLRLRIRRAGGQDATSQVD TSTAPVHSSHN</aaseq> |
| Gene Sequence |
<dnaseqindica>111..465#8340..9718#aagggcgaggtgagcgaggcgagactcgagagagaggagaagatccagaaatcgcaagctgcacgctatagctaggtagctatctccggggaaagagagagtaggctgccatggagagcttcttcgtcttcttcacggcggcggcgttgccggtggtggtggcggcggcggtgatcgccgggctgtgcattacggcggcgtggctggcgaggccgcggcgcgtggcggaggtgttccggaggcaggggatcgacggcccgccgccgtcgtcgttcctggcggggaacctcccggagatgaaggcgagggtggccgccgcggcgtcggcggcggcgccaacggcggacggggaggagaccgcctccgccggcggcggcggcggtggccgggacttcgagaaggacgggttcgacgactactgcaccaggatcttcccttacttccacaagtggaggaaagcctacggtacgtgtacacaagcctctctcctctctactgctcgatttcacatcactagctacatgcatgatgcttgcttgctttgcctgttaattactccgctcatgtgtgcttaattagcttaattaattcacactccggttaatcaagtcatgcaaaacccacaattaatcagctatcaattgagatgagtatataccagaatatatatacttacagtaaatttaataaaaaaataaaaaaaagaaaagaaaagctctctcgatctctctgtgacgatgatacaagaaatttgtggttgccctagtaacaagttgactagagtataaaagctgctaaaatgtcaagagctctccatataaaaaagaacagtgggggagaataattaatactgtcccaaacagcgagaggaagagagggggcaaacgagaaaagagagaataaacaaaattaatgcaggatggaaaccgtggttgggacgaactactcggtttgcttccagtcaaccgtcctgcatcattaagctatggattaataattaattaactgcagtatataaatatatatactcccccacatatattaatcggtcgttggtccaattagacaacagcaaagccatgcctaggtacctgtctaggtaggctatgactgtagtacactcgtactactcacttaatagcacattagtgctcttattttgcgaattaatgaaactttaatcaataataactatatataactataatatatttagtataatttaacaatatatctattcagcgtgctatcgtttcttcagcctatcaatcaacttaaaaaacttagtgttaaattgattttggtgttttcttatcgtaatttttcttcagttttggattgttttttaagttgctaacaattttagttaacaaagctatttggaaggagaggaataatgcatccatgacaaaagtatatgcttcataataattttcatataccagatcttgcccctggtggtgacgtgttaccaaataccgactggaattaatttcttctctctttctttttctctctgccggtgcaatgaggcgacatttttgagttataactgtgcgtatgaatgcatgtattaattgctgtttgctgaggttaataggctagcaataccgttgttttgggggaccccgcagtttgacctagtagagtttatcatggacgcagactgcccctacctgttcttcttcacggacagcatgattaattagctatactagtggtagtactcgatgtcattaataattggtctctccgtacgtgaataattatcgatttttgactttgacaagtactccctccgtacttgttaaggaagtcgttttagacagcaacacggtctccaaaacacaactttaacttcttatttctataaaaatatttattgaaaagtgatatatgtatacttttatgaaatattttttaagacaaatatattcatataatttttacattttaaaacttaatggaaagttacttttccaagatttgactcaaacattgttctaaacgatttcctttatgagtacggaaaaagtatgttttttcactgttcttatgaagtgtacatggaaagtgatcgaatagctctgagcagtactctaggtagggaattacttttagttgcgtacaaatctaacaattaattaatatgccatttttagtgaacataattgtatattttctccgtttcacaatgtaagacattctagcattttccacatgcatattaatgttaatatgtctagattcattaacatcaatataaatataagaaatgctagaatgacttacattataaaacggaggaagtaatttatgctagaatgacttacattataaaacggaggaagtaatttagtacatcaccttcgtcgattattacgaaaacatccatcatatcgatcgatatctgtgtatttgctactccagaaaaatattttctgagaccgacccacagatgcatatatatgcacattattgtggcggttttcctttctttttagatcaatcggtggcctgacggccggcccagcttcgattgaaattaagctgaataattattgtggccgtcatgtgctaatcacccatataattaggcgtggttagaacctgactaagctatttaagccggtcagttaggctttcccaaaacggccatcaggccatgcatgttgccattagctgtccgagcacgccggccaaagctcaaccctcgtgatcacatcaactcgatctccccgatgcttatccctttttgcaagcaaccggtatgtggtgcttagataattaatgcaagaattaacagtgtacctcctatcaatttgccgttagctatgcatgtaatggattagaaattctatatgcatgttttcgtctaacagagtttgatcagcagcagtacttagctaactaattattctgtaatttatataactactataagctagagtttaagcagagtatgtaagtatgatattttaactaatttagttcttgtcatatggcactgtagttgaaaccataaaccgatcaattaataggacaaatattcaacctggttgcctcactgcatgcgttgttttagtttgacaaatgcatgcatgtgtatgatattttatttgcttggcccttaataagtatattatgttgaagtcatgcatcttgacacagaaacttggagcaaaaactatataatatatttcaagaaccaaagggcctgaatactagtacttgcctacatcgatcacatgatgctagtgtcaatcttatttaaggtttttatttcttctttaatgtcaaaatggctattgtttaactatctaaaaacaccaataattagaagactcccactagaccatctcatgatgcattgcacgtaggatcgatatatatggcaacattaattattttacttgaacatctaatcgcgttcgctattattcaacttatgttacatgttctcccctaatttaagcaaaggccggtttgaccaacgggaaacaaataattaaattgatcttccaggcccagtacttatattaattacctagtcattaactacattttctccatatttaatttgaatgcttcgttctgaaatatttaaaggccattaacactaacacggcacacaagaaattaatttaaatatttaatttggctagcatgtatacatatatatgttgatatgcatggatatatatatatatatatatatatatatatatatatatatgtatatatatatatatgtatatatatgtatatatgtatgtatatgtatatgtatatgtatatgtgtgtgtgtgtgtgtgtgtgtgacaatagctaactttagatatgtttttttttcgatgatacatggtcccggcctctgcatcacaaatgcacacatccaacgaatatcgtacaaataataataaaaaaagtgatgggacaaaactcccgactcctacataagacttaggactagtgagctaagggtgtgtttagttcaccaaaattggaaatttgattgaaattagaatgatgtgacggaaaagttgaaagtttgtgtgtgtaggaaagttttgatgtgatggaaaatttggaagtttgaagaaaaagtttagaactaaactcggcctaactgaaaacaaatcaattgatatttgttttcagttatgccgagtttagtttgacaaaattaaacgaaaccatacaatatataattacaaatcaataattaattcctaatgctgcttgatgctactagcttctctcctacacagtgccattgactaggtaattgctcctaaccctagctagctaagcatgcagctcgatcgaatcgatcgagcatgaaagcaacaggtcgatcgatagatggtaagccatgacgatcaatatgatcatatcatgcatgcatgacactgtagagtgtgtactacgtactaccaccactccaccaaccatgcatgcattcctcccccctctctcttgcaccttttaaagctagctaggacgtgtggggacttcaatatatttcttgtgcattgtctctcctacaaggattccacatggagatgtgcatatgcatggggttacaccaattaaaagtgcagcccacactctatggattgaaggtgctagctagatctctgtactctatccgtcccaaaatataagcatttttagttataaatccagatagatagttatccagattcgtagctaaaagttattatattttaggatggaggtaatatagtttagatggtggaaattaagtctagtagctatggggaaaaatggcaaaaataaaattacagtatggggttgtactggaaaattttcatttgaatatatctcttcacaaatatgtaagcctaaatttaatctaaatatatatgggcacaaatgaattaaaaaacatattttctatgaacatatatagcaggaataatatgcattcctcgattttattcccttcaagtgttcatatagtacgttaaaagtttgggttaaaaattttaaataatttctttagttattttgaaatggtatgaatgaaataagagcgtatacccttaagtcataaaagatcatccagattgaataaaaaaacaaagttaagataattaagtagatgaatgtaaatgtgcatatacatatcttgtctttctgttgactagttgtctgttcagaaattatatggcggaaaccctaactgaggtgaaaattcatgtgtaccaagacaaaaaaagaaagttctgaaatcacacaaacaatctcataagtagataagatgatagtgaacaaccatgtaaagatcttttccaaatttaactttcatttgtcagatataaaatgacaaattttgaatcagaaaatattttttttgtttgaaatttaccctcgtttcttgatctgaacagtaccactagatacacctagctacatgtatggtcgattgtgataggctcacacacatgctatagcttgatggttgtcatggtggttgcctagttggcaaaagttcgatgtccagctgtgctactatcatgataattggcagcagcctagctagctagttcaaggggacaatattacactggctttaataatttcctcgtcaccaccttttacctcaaaaacatattcttgaaaaaatgaagaatatgatcatttcaccaaaaatcatggactggccatgattaaccaactcattttggatggttttgttggattcctcaaaccagacacatcattctctgtgtcgagaatggagatgacgtgactagcttttatgactttagaaacatactccctccgtctcaaaaaataaactttaggtttctgtgtccaatgtttgatcgtatatcttatttgaaaaaaatatgaaaaaaattaaaaagacaaatcacgcataaagtattaatcatattttatcatctaacaataattcttttcgtgcttgtgattgtgagtattggaattcttttaatatatgctgaatatactaatgagcataaagtataacagactaatcaagtatgtgagttattcaatccttggcaatttccaatacaaatacagacacactatgtatggaacttttttatatatagtctattaaacaatatatctttgagccgtctgaaaccaaattatatgatatttttttagttaatcataccaagatcattaattgagtaggaatataagacttcaagggggaatcaacaatattaattgtttctgagaacaattattcaaagcaattcataataaaagcatatttattttaggagtgggtacacacactatctagcttgtaccatatataagtcaatgaccacgatatatggctagccacctccctccatcatgaaataaattaacctcatacgaatttagatatagaatatgtccagatttattgattaaatcctagattgatttattttaggacgaagagattacctgcctgcctgcactaaccatctgcgacgccgacgtgtcatccatcggtcgacaaaacgtacgcaggccaacacatgcatacgtacacacaattgcccatttgcaccccgctccccatgtccacgtcagacggcgacacgtacacattaacacatgtacacgtcgttttcacgtacgagctaagcaaatccaacgtacagaaaatagttaatttacccggttgccaactgggacacgtacgccggccacgtcacaggtctacgtggcgcggtgtgggccgggctaggagggttaaaagcccacgcttgtgtgctttggtcaactcgtgcacttattactctgaagagttattagccggttcgatgtgtcgcgattactgttgtaattaagtctgtctctgtggcgatatgtacgttgacgatgattaaggtggtgtttggatctagagacttaactttagtctctgtatttagacactaatttaaagtattaaatatagactaattacaaaactaattacataaataaaaacttatttgcgagataattttttttaagcctaattaatccataattagaaaatatttaatgtagcatcacataggataatcatgggttaattaggctcaatagaatcgtctcgcgaattagtctaagattatggatagtttttattaatagtctacgtttaatatatataattagtgtccaaacatccgataagatagagacttaaaagttttaggtggtgtttagatctagggacttaactttagtccctatatttagacactaatttagagtattaaatatagactacttacaaaactaattacataaataaaagctaattcgcgagataatttttttaagcctaattaatatataattagagaatgtttactgtagcatcacatatgctaatcatggattaattaggctcaatagattcgtctcgcgaattagttcaagattatggatgggttttattaataatctacgtttaatatttataattagtgtccaaacatccgatatgatagggacttaaaagttttaatctcatctaaatatggttttagtctcatctaaacagggtctaatagcagaaagttacgtgagattcgtatactcgtacgtacgtatttgcatcggaattttcaaaatcctagactctttctagtactggtggtacagtttggcaaatctggttttttggatggtttcagcatgtttaatttgcagtgtggaattgttgagatgctagagattttttgcagaacttatgtgaaatttaattaggcgtttcaactgtggctaaacgtgaagatatagcgcgctagagacgtgtgtgttggcagtgttgctttgcaatttgcataggctcgtggtgtttggttcagaaatttatttaaggatccggttgttttttttttcattttccttggtcaatgttgtaaagaatccgtagaatcttggcaagaagattctcccaaacctccaggaaagaggagaagattctccggaatcagtggaagtagtgcagtagctattgactgggatttttgtaagcccggtcaataggttcccctgtacccttggagagaccctgcaagtgcacacagatcacgtatatatccacatgcctatatgtgcatagatcacataggcacgcatcaaaagctttttaatcctagctagcttactttaatctttgattattttcaatagaaatttaatgacgtttaacttgattcatcattcggcgacgtctcttgaacatgttaaaggctgggccgaggcccaccaaaaacggaggcccatgagcaaatttcaaattctgactcgcgcgcggtggcggcaaaattttgcaggcgagacgtacctgtactggctgcggcggcggccggcgctgtacgtgacggacccggagctcatcggcgagatcgggcggtgcgtgtcgctcgacatgggcaagcccaagtacctccagaaaggccaggagccactcttcggcggcggcgtcctcaaggccaacggcgcgtgctgggcgcgccagcgcaaggtcatcgcgccggagttctacatggcccgtgtcagggccatggtccagctcatggtcgacgccgcgcagccgctgatcgcctcctgggaatccaggatcgacgccgctggaggcgcggcggcggcggaggtcgtcgtcgacggcgacctccggagcttctccttcgatgtgatatcgcgggcttgctttgggagtgattactcgagggggagggagatcttcctccgcctccgtgagctgtccgggctcatgtcggagaccagcgtcatcttcagcatcccttcgctgaggcacctgccgacggggaagaaccggaggatctggaggctcacgggggagatccggtcgctgatcatggagctcgtcagggagcggaggtgcgcggcgagggcggcgagggagcacggcgggaaggcggcgccgccgtcgccgccggagcgcgacttcctcggctccatcatcgagaacagcggcgggcagccgcggccggacgacttcgtggtggacaactgcaagaacatctacttcgccgggcacgagacgagcgcggtcaccgcgacgtggtgcctcatgctgctcgccgcgcacccggagtggcaggaccgcgcccgcgccgaggtgctcgaggtctgcggcggcgacggcgccgccgcccccgccgcgccggacttcgacatggtgtcccggatgcggacggtggggatggtggtgcaggaaacgctgcggctgttcccgccgtcgtcgttcgtggtgcgggagacgttccgggacatgcagttgggtaggctgctggcgcccaagggcacctacctgttcgtcccggtgtccaccatgcaccacgacgtcgccgcctggggcccgacggcgaggctgttcgacccgtcccgcttccgcgacggcgtggcggcggcgtgcaagcacccgcaggcgtcgttcatgccgttcggcctcggcgcccgcacctgcctcggccagaacctcgcgctcgtcgaggtcaagacgctcgtcgccgtcgtcctcgcccggttcgagttcacgctctcgccggagtacaggcactcgccggcgttccggctcatcatcgagccggagttcggcctccgcctccgcatccgccgcgccggcggtcaggacgccacgtcacaagttgacacatctactgcacccgtgcatagttctcataattaattggcttctgtaaacagctagtaagtcgtgaactgaaaaaaatacatcacacatacagagacaacagaggattaccattttaccagc</dnaseqindica> 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| External Link(s) |
