Difference between revisions of "Os01g0742500"
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==Labs working on this gene== | ==Labs working on this gene== | ||
| − | Plant Metabolism Research Center and Graduate School of Biotechnology | + | 1.Plant Metabolism Research Center and Graduate School of Biotechnology |
| − | Department of Biology, Chungnam National University | + | 2.Department of Biology, Chungnam National University |
| − | Department of Horticultural Biotechnology, Kyung Hee University | + | 3.Department of Horticultural Biotechnology, Kyung Hee University |
| − | Department of Life Sciences, Pohang University of Science and Technology | + | 4.Department of Life Sciences, Pohang University of Science and Technology |
| − | Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts | + | 5.Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts |
| − | Plant Metabolism Research Center | + | 6.Plant Metabolism Research Center |
| − | Graduate School of Biotechnology, Kyung Hee University | + | 7.Graduate School of Biotechnology, Kyung Hee University |
| − | Division of Molecular and Life Sciences, Pohang University of Science and Technology | + | 8.Division of Molecular and Life Sciences, Pohang University of Science and Technology |
==References== | ==References== | ||
Revision as of 16:39, 24 May 2014
Please input one-sentence summary here.
Contents
Annotated Information
Introduction
Hexokinase(HXK) plays a significant role in Glucose metabolism and Glucose signal transduction. Rice has ten HXK genes including OsHXK1~10[1][2]. The hexokinase 1 (AtHXK1) is recognized as an important glucose (Glc) sensor. It was also proved that OsHXK5 and OsHXK6 are evolutionarily related to AtHXK1.
Function
These OsHXKs including OsHXK6 retain a dual-targeting ability to mitochondria and nuclei. Some results also provide evidence that rice OsHXK5 and OsHXK6 can function as Glc sensors.
Expression
Transgenic rice plants overexpressing OsHXK5 or OsHXK6 exhibited hypersensitive plant growth retardation and enhanced repression of the photosynthetic gene RbcS in response to Glc treatment.
Mutation
The expression of OsHXK5, OsHXK6, or their mutant alleles complemented the glucose insensitive2-1 mutant, thereby resulting in wild-type characteristics in seedling development, Glc-dependent gene expression, and plant growth.
Orientation
Transient expression analyses using GFP fusion constructs revealed that OsHXK5 and OsHXK6 are associated with mitochondria.
Labs working on this gene
1.Plant Metabolism Research Center and Graduate School of Biotechnology 2.Department of Biology, Chungnam National University 3.Department of Horticultural Biotechnology, Kyung Hee University 4.Department of Life Sciences, Pohang University of Science and Technology 5.Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, Massachusetts 6.Plant Metabolism Research Center 7.Graduate School of Biotechnology, Kyung Hee University 8.Division of Molecular and Life Sciences, Pohang University of Science and Technology
References
Please input cited references here.
Structured Information
| Gene Name |
Os01g0742500 |
|---|---|
| Description |
Similar to Hexokinase |
| Version |
NM_001050749.1 GI:115439868 GeneID:4326547 |
| Length |
4329 bp |
| Definition |
Oryza sativa Japonica Group Os01g0742500, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 1:32765472..32769800 |
| Sequence Coding Region |
32765921..32766268,32766361..32766442,32766656..32766786,32766860..32766958,32767099..32767254 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008394:32765472..32769800 source=RiceChromosome01 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008394:32765472..32769800 source=RiceChromosome01 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggggaaggggacggtagtggggacggcggtggtggtgtgcgctgcagcggccgcggcggttggggtggcggtggtggtgtcgcggaggaggaggagcaagcgggaggcggaggaggagcggcggaggagggccgccgctgtgatcgaggaggtggagcagaggttctcgacgcccacggcgctgctgcgcggcatcgcggacgccatggtggaggagatggagcgcggcctccgcgccgaccctcacgccccgctcaagatgctcatcagctacgtcgacaacctccccaccggggatgagcacggactgttctatgctctcgatcttgggggcaccaatttccgtgttatacgtgttcagcttggaggaagggaaaagcgtgttgttagtcaacagtacgaagaggttgccattccacctcacctgatggttgggacttctatggaactgtttgacttcattgcggctgagttggaaagttttgtcaagaccgagggagaggatttccacttgccagagggcaggcagagagagttaggcttcaccttttctttcccagtgcaccaaacatcaatatcatcaggcactcttattaagtggacaaagggattttccatcaatggcacggtgggggaagatgttgtggctgaattgagcagggctatggaaaggcaagggcttgatatgaaagttacagctcttgttaatgacactgtaggcacattggctggcggaagatatgttgataatgacgttgctgctgctgtaatattaggcactggcacaaacgcagcctacgtggagcatgcaaatgcaattccaaaatggactggattactacctagatcaggaaatatggtgattaacatggaatggggaaacttcaagtcagaaaggcttcctcgttcagattacgataatgccttggactttgaaagtttaaacccaggcgagcagatatatgaaaagatgatttccggcatgtatcttggagagattgtgcgcagaatcttgcttaagcttgctcatgatgcttccttgtttggagatgttgttccaacaaagctggagcagcgctttatactgaggacgccggacatgtcagcgatgcatcatgatacctcacatgatctgaaacacctgggagctaagctgaaggatatcctgggggtcgctgatacttccctggaagcacgatacatcacccttcatgtctgcgacctcgttgcagagagaggtgcacgcttagctgctgctggtatatatggcattctaaagaagctgggcagggacagagtgccaagtgacggtagtcaaaagcagaggactgtcattgctctggatggtggtctctatgagcattacaagaagttcagaacctgcctagaagcaacgcttgcagacctgcttggagaggaggctgcctcatcagttgttgtcaagttggcaaacgatggctctggcatcggagctgcacttcttgcagcatctcactcccagtatgctagcgtcgagtag</cdnaseq> |
| Protein Sequence |
<aaseq>MGKGTVVGTAVVVCAAAAAAVGVAVVVSRRRRSKREAEEERRRR AAAVIEEVEQRFSTPTALLRGIADAMVEEMERGLRADPHAPLKMLISYVDNLPTGDEH GLFYALDLGGTNFRVIRVQLGGREKRVVSQQYEEVAIPPHLMVGTSMELFDFIAAELE SFVKTEGEDFHLPEGRQRELGFTFSFPVHQTSISSGTLIKWTKGFSINGTVGEDVVAE LSRAMERQGLDMKVTALVNDTVGTLAGGRYVDNDVAAAVILGTGTNAAYVEHANAIPK WTGLLPRSGNMVINMEWGNFKSERLPRSDYDNALDFESLNPGEQIYEKMISGMYLGEI VRRILLKLAHDASLFGDVVPTKLEQRFILRTPDMSAMHHDTSHDLKHLGAKLKDILGV ADTSLEARYITLHVCDLVAERGARLAAAGIYGILKKLGRDRVPSDGSQKQRTVIALDG GLYEHYKKFRTCLEATLADLLGEEAASSVVVKLANDGSGIGAALLAASHSQYASVE</aaseq> |
| Gene Sequence |
<dnaseqindica>3533..3880#3359..3440#3015..3145#2843..2941#2547..2702#2262..2336#1477..1659#1230..1380#186..481#agaggagagagactgagggagggaggcaggaggaaggaggaggaggaggacgctgcagtggtgggtggcgtagctcccgatccgggaagccgacccggtctgggggatttgctcctggcgcgcgctcgatcgagagggaggccagggttgggttggggggagcgtgaagaagcgcgcgcgcggctatggggaaggggacggtagtggggacggcggtggtggtgtgcgctgcagcggccgcggcggttggggtggcggtggtggtgtcgcggaggaggaggagcaagcgggaggcggaggaggagcggcggaggagggccgccgctgtgatcgaggaggtggagcagaggttctcgacgcccacggcgctgctgcgcggcatcgcggacgccatggtggaggagatggagcgcggcctccgcgccgaccctcacgccccgctcaagatgctcatcagctacgtcgacaacctccccaccgggtaggcctccctccgctccgcttgtgcgatttttatgatttctggggtgtacgtgacacgagaaagcttgggaatagttgttactgtgttcgcgaggggtgtttctcttcgggggcgaatcggttggaaatccctagcggcttagcgcttgcttaggttcggatttggcataaatgtggcattgatcttcggttgttgagatggaggtgcgacatattgatatcaagagcactgtgccttgacacggccttttagctcttttcttattgatcacggaagaatgatgagacctattcgtttcctatggaattttgatctactgagtggaggtttctcacggtggtagcagaaagtggccaccaggatagacttgacataatttttttttcgcagtgaagactccagacagaattataaaatgaacaattaggttccggtcacttttgacaactatatatatctgtgtgtccattttgtaatttgtgccttcatgctgggcttacgatcagtcgatcacagaacaatcgttcttttttttgtaccatgtgaattttgtaggaaatgtcatgaaaggtttatgaataagtttagagaaagaaatggtcaaggactcgaggcttaaaagtttcagtgagtttgacagtttattatatgtatccataggcgtatggaaaatgaaaaatgttgagcctatcacttcatgaaatcttcttttatgcttcctctgatttgcatgccacttgtgcagggatgagcacggactgttctatgctctcgatcttgggggcaccaatttccgtgttatacgtgttcagcttggaggaagggaaaagcgtgttgttagtcaacagtacgaagaggttgccattccacctcacctgatggttgggacttctatggtcagtggcttattcctttcaactgttatgtttcaattctcctgtgtttgcacaatttctttcatggttactcaaatttcagaaatatgagtacaggaactgtttgacttcattgcggctgagttggaaagttttgtcaagaccgagggagaggatttccacttgccagagggcaggcagagagagttaggcttcaccttttctttcccagtgcaccaaacatcaatatcatcaggcactcttattaagtggacaaagggattttccatcaatggcacggtaaaattcgaatggccttagtataaatttcatggctaaatgatctaggaattatttattaccttgacgatcagtcttgattagaaaccacgttgtgtcagcaaacgaattctcactagctgtgtcacttttgacttttgggttccctgcattgaccaagtgaccagccgttgcccttcattttcctttcagaatatttggactgatgtttggttatccataaattaacatgtttggtaaagttattcttagatgtgatataaacgggacatggtggcatatgtccccatttatccaattcacaagtatcttataattgcttctaaatcctgcggcgattgtgaatgcagatgttacaattagagctgtgtttgctatacaacacttgtaaccataaaaaaatgtatttgcttatcctgcacttcttaaatttgaacctccaagaatgaagcactctagtcatatttctgtttcctacttttaatgtggcaatttctagcctctcaagacaaatgtggcatcaacactgcaatatcttttttaattttataatgttatgcccataattttgatgtatagactaatgatttctgtgaactcaggtgggggaagatgttgtggctgaattgagcagggctatggaaaggcaagggcttgatatgaaagttacagctcttgtaagtaaattcttggttcatgactctgttaaaattatttatcatctgaaactttcccatgcctgctactgctatatgctgatgggtaaatacattggcattcctatgaaaatgctgactttggagtttttcaatgttctatgagtttggatgtttactagtatatcaccagactgtgtcatgtaaattgacaactaatattttgcccaggttaatgacactgtaggcacattggctggcggaagatatgttgataatgacgttgctgctgctgtaatattaggcactggcacaaacgcagcctacgtggagcatgcaaatgcaattccaaaatggactggattactacctagatcaggaaatatggttagtgactgatttcgttatacttgtagcattaaatgtctttgaaccttgtataaatgtatgtatccacttcaacttctgcactggatgattgtcacatcaattaatattataacatttggatctttggtatgatgaaggtgattaacatggaatggggaaacttcaagtcagaaaggcttcctcgttcagattacgataatgccttggactttgaaagtttaaacccaggcgagcaggtattgtttattttatctttttattttccaactatatgtgggcatgaaagaatttcggtcttcttttcctcagatatatgaaaagatgatttccggcatgtatcttggagagattgtgcgcagaatcttgcttaagcttgctcatgatgcttccttgtttggagatgttgttccaacaaagctggagcagcgctttatactgaggtgcacttctatgtcccctatgttgatcatctaagtatctagcttttcatatatgcagcacagaactatcaaaataagataaaatttcaacatagactgtatggtgtgcattatgcgttccttgatcatggtagcacagaactatcaaaataagataaattcatgtaactatgtagaaaatttcttaacggaactccaattgtggtattgcaggacgccggacatgtcagcgatgcatcatgatacctcacatgatctgaaacacctgggagctaagctgaaggatatcctgggggtatgataatacatatcttgaaaataggatactatcaaatctaattttcaagaaccattgattacttacgatatgcctgaacttatgtttaggtcgctgatacttccctggaagcacgatacatcacccttcatgtctgcgacctcgttgcagagagaggtgcacgcttagctgctgctggtatatatggcattctaaagaagctgggcagggacagagtgccaagtgacggtagtcaaaagcagaggactgtcattgctctggatggtggtctctatgagcattacaagaagttcagaacctgcctagaagcaacgcttgcagacctgcttggagaggaggctgcctcatcagttgttgtcaagttggcaaacgatggctctggcatcggagctgcacttcttgcagcatctcactcccagtatgctagcgtcgagtagtaacaggagctcatgggactgagctcccagtgtagcttgttttcctcccattttccccgtttctttccaatgggagttcgtttccctcctgcgattcgcatctccttttgctattctgcagtcacataaacgagtgcctgtgcagcgggatgtagctagtatggcgccaaagagtttgcagttatcacatgaacaagcatttgcaactgcagggaagtgaaaacgggggcttgaatgatgccgttcttttcctgcaaattattttccccctttccctgtaagtttgtattgtgatgcgatgtcgcaaaccaatcacagcggtttcgcgtgtagccttttgtcattcagatttcagaataaagagggggacataatttcacttttcgtatgtcagtttgaactatgtatgtactcttacacactggtatcaaagctcaaatgatctgatc</dnaseqindica> |
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