Difference between revisions of "Os03g0848700"
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===Expression=== | ===Expression=== | ||
| + | Because BPH insects usually aggregate on the lower parts of rice plants and ingest the phloem sap, we thus studied the tissue specificity ofBph14expression. We found that Bph14was expressed constitutively in leaf sheaths, leaf blades, and roots (Fig. 2C). Then we examined Bph14 activity in more detail, using transgenic plants carrying the fusion construct of theBph14promoter region and theGUSreporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades (Fig. 2E, G,andI). In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering xylem vessels and sieve tubes (Fig. 2F, H, andJ). Such an expression pattern is consistent with the role of Bph14in recognizing BPH attack in phloem cells. To determine the subcellular localization of the Bph14 protein, the coding | ||
| + | region of Bph14fused to modified green f luorescent protein (GFP) at the N-terminal end was expressed under the control of the CaMV 35Spromoter. Fluorescence was detectable in the cytoplasm in onion epidermal cells following particle bombardment (Fig. 2D). | ||
| + | |||
Bph14 have been identified in B5 and mapped on the long arm of rice chromosome 3【2】(B5 is a rice line derived from the wild rice Oryza officinalis and is highly resistant to the BPH biotypes 1 and 2.).Bph14 is predominantly expressed in vascular bundles,the site of BPH feeding.Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein | Bph14 have been identified in B5 and mapped on the long arm of rice chromosome 3【2】(B5 is a rice line derived from the wild rice Oryza officinalis and is highly resistant to the BPH biotypes 1 and 2.).Bph14 is predominantly expressed in vascular bundles,the site of BPH feeding.Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein | ||
Revision as of 07:02, 25 May 2014
Bph14 is a gene conferring resistance to Brown planthopper (BPH) at seedling and maturity stages of the rice plant.
Contents
Annotated Information
Function
Bph14 was first mapped from the introgression line B5, derived from O. officinalis, with Qbp1 (renamed as Bph14) on chromosome 3 explaining 26.4% of the phenotypic variance of BPH resistance in this population[2]. Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein. Sequence comparison indicates that Bph14 carries a unique LRR domain that might function in recognition of the BPH insect invasion and activating the defense response. Bph14 is predominantly expressed in vascular bundles, the site of BPH feeding[1]. Expression of Bph14 activates the salicylic acid signalling pathway and induces callose deposition in phloem cells and trypsin inhibitor production after planthopper infestation, thus shows antibiosis and antixenosis effects on BPH, like delaying the development of males and decreasing the female ratio and copulation rate, as well as the antixenosis action against BPH nymphs, female adults and ovipositon, and what's more, some P450 members of the CYP4 and CYP6 family in BPH are inducible by host allelochemicals, like CYP6AX1, CYP6AY1, CYP4C61, CYP4C62, CYP6CS1, CYP6CW1 and CYP303A1[3]. Bph14 showed a stable resistance in different genetic backgrounds and thus is valuable in development of resistant rice varieties[1].
GO assignment(s): GO:0009625
Expression
Because BPH insects usually aggregate on the lower parts of rice plants and ingest the phloem sap, we thus studied the tissue specificity ofBph14expression. We found that Bph14was expressed constitutively in leaf sheaths, leaf blades, and roots (Fig. 2C). Then we examined Bph14 activity in more detail, using transgenic plants carrying the fusion construct of theBph14promoter region and theGUSreporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades (Fig. 2E, G,andI). In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering xylem vessels and sieve tubes (Fig. 2F, H, andJ). Such an expression pattern is consistent with the role of Bph14in recognizing BPH attack in phloem cells. To determine the subcellular localization of the Bph14 protein, the coding region of Bph14fused to modified green f luorescent protein (GFP) at the N-terminal end was expressed under the control of the CaMV 35Spromoter. Fluorescence was detectable in the cytoplasm in onion epidermal cells following particle bombardment (Fig. 2D).
Bph14 have been identified in B5 and mapped on the long arm of rice chromosome 3【2】(B5 is a rice line derived from the wild rice Oryza officinalis and is highly resistant to the BPH biotypes 1 and 2.).Bph14 is predominantly expressed in vascular bundles,the site of BPH feeding.Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein
| Primer | Forward primer | Reverse primer |
|---|---|---|
| Gene amplication | 5'-GGCGACTGCGAATGCTAT-3' | 5'-GGCAGATCATCACCAACTCC-3' (used to amplify Bph14 [4]) |
| 5'-CTACAGGCAGCCAGCAGAT-3' | 5'-TCCTGTCAGATTCTTGCACTG-3' (used to amplify LRR domain [4]) |
Evolution
Bph14 shares 83% sequence identity with its allele (Os03g0848700) in Nipponbare. Phylogenetic analysis revealed that Bph14 is closely related to other rice homologs and is divergent from the majority of known plant disease resistance proteins in other species. See the picture of phylogenetic analysis on the right hand.
Labs working on this gene
- Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China
- National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China
- Shandong Rice Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China
- College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
References
- ↑ 1.0 1.1 1.2 1.3 Bo Dua, Weilin Zhanga,Bingfang Liua, et al. (2009) Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice.Proceedings of the National Academy of Sciences, 106(52): 22163-22168.
- ↑ Huang Z, He GC, Shu LH, et al. (2001) Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet 102:929–934.
- ↑ Jie Li, Qiuhong Chen, Liangquan Wang, et al. (2011) Biological effects of rice harbouring Bph14 and Bph15 on brown planthopper, Nilaparvata lugens. Pest Management Science, 67:528–534.
- ↑ 4.0 4.1 Zhou Lei, Zhijun Chen, Xuyong Lang, et al. (2013) Development and validation of a PCR-based functional marker system for the brown planthopper resistance gene Bph14 in rice. Breeding Science, 63:347–352.
Structured Information
| Gene Name |
Os03g0848700 |
|---|---|
| Description |
Similar to TDRGA-1 (Fragment) |
| Version |
NM_001058430.1 GI:115456588 GeneID:4334783 |
| Length |
5725 bp |
| Definition |
Oryza sativa Japonica Group Os03g0848700, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 3:36544018..36549742 |
| Sequence Coding Region |
36544479..36544552,36545320..36549226 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008396:36544018..36549742 source=RiceChromosome03 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008396:36544018..36549742 source=RiceChromosome03 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>atggcagagctgatggccaccatggtggtcgggccactgctgtccatggtgaaggacaaggcctccagctacctcctggagcagtacaaggtcatggagggcatggaggagcagcatgagattctcaaacgcaagctgcccgccatcctcgacgtcatcgccgacgccgaggagcaggcggcaaaacacagggaaggggcgaaagcatggcttgaggagctccggaaggtggcctaccaggccaatgatgtcttcgacgagttcaagtacgaggcactccgccgcaaagccaaggccaaggggcactacaagaagctcggcagcattgttgttataaagctcattcctactcacaaccgtattctgtttcgttataggatgggcaacaagctcaggatgatcctgaatgccattgaagtcctaattgcagagatgaatgcctttaggtttaagttccgaccagagccaccaatgtcctccatcaaatggagaaagacggattctaaaatctccaacctttctatggacattgccaacaagtcaagaaagaaagacaaagaggagattgtcaatcgattgcttgctcaagccagcaatggggatctcactgttattcccattgtaggaatgggggggatgggcaagaccaccttagcgcagctcgtttacaatgaccctgaaattcagaagcattttcagttgctgctctggttgtgtgtctctgacaacttcgatgtggattcgctggcaaaaagaatagttgaagcagctcccaaagaaatgaataagaagaatgataatggaggggccaaaaagttaccacaggatgaacttaaagaagttgtgagtgggcagaggtacctcctcattttggatgatgtctggaaccgtgatgccagtaagtgggaagcactcaagtacaaccttaagcatggtggcagcggtagctcagttttgacaacaactcgtgatcaagcagttgctcaactaatggctccagctcaagaagtttatgatctcaagaacctgaatgaaagcttcattgaggaaattatcaagagaagtgctttcaattcagaacaagaaaggcctcctcctgagctactcgaaatggttggtgatattgccaagaaatgttctggttcccctttagctgcaacagcattgggctctacactgcgtacgaagaccaccaagaaagaatgggacgctatattgagcagaagcacaatttgcgatgaggaaaatggaattttaccaatactcaaactcagttacaattgcttgccatcatatatgcggcaatgtttttccttctgtgctatttttcccaaggatcatgagatcgacgtggaaatgctgatccagttatggatggccaatggttttatcccggagaaacaaggagagtgccctgaaatcattggtaaaagaattttcagtgagttggtgtcaaggtcattttttcaggatgtgaaggggatcccatttgagttccatgatataaagtgctctaagattacttgtaagatccatgaccttatgcatgatgttgcacaatcttccatgggaaaagaatgcgctactatagctacagaacttagtaaaagtgatgattttccttattccgctcgccacttgtttttctcaggtgtcattttcctaaagaaagtatatccaggtatccaaacattgatatgttcctcgcaagaagaattgatacgttcctcgcgagaaatatcaaaatacagttcattgcgagctttaaagatggggggagattcattcctgaaaccaaaatatcttcatcacctgaggtatcttgatttgtcatacagtaaaattgaagcacttcctgaagacataagcatcctatatcatctgcaaacattgaacctttccatttgtgactgtctttgtcaactcccaaacggaatgaagtacatgaccgccctccgtcacttgtacactcacggatgttggaggttaaaaagcatgcctccggacctcggacacctcacttgcctacagacgcttacatgttttgtagccggttcttgctctggctgcagtgatttgggagagctgcggcagttggaccttggtggtcgactagagctaagaaaactggaaaatgtgacaaaagctgatgcaaaagcagcaaatctcggaaagaaggaaaaactgaccgaattgagcttaagatggactggtcagaagtacaaggaggcacagagtaataatcataaagaggtgctggaaggtctcacgcctcacgaggggctcaaggttctgagtatacttcactgtgggagcagtacatgtccaacatggatgaataaactgcgggacatggtgaagcttgtgttagatggttgcaaaaatctcgagaagcttcctccactgtggcagctgccagctctagaagttctttgccttgaaggactggatggtttaaattgcttgttcaactgtgacatatacacctcctttacattttgcagactgaaggagcttaccttggccagtatgagaaattttgagacatggtgggacacaaatgaggtaaaaggagaagagctgatatttcctgaggttgaaaagctgataatcaaaagttgtcctaggctaactgccttaccaaaagcgtcaaatgtgatttcagaattgtctggcggagttagcaccgtgtgtcattctgcatttccagcattgaaggaaatggaattatatggtttggatatctttcagaaatgggaggcagtcgatggaactccaagggaggaggtaacatttcctcagctttacaaattagatattagacggtgcccagagttgactactctacctgaagcaccaaagctgagagacttaaacatatatgaagtcaatcagcaaatatccctacaggcagccagcagatatattacttcattgtccagtctccacctgcatttgtcaactgatgacacagaaacagcaccggtggccaagcaacaagattcgagtgaattggtgattgaggatgagaaatggaatcataaatctcccctggaacttatggacttaactggctgcaaccttttattctcttacccaagtgcgttggctctgtggacatgttttgttcagctcctagatctgaatatttgggaagtcgatgcgcttgtcgactggccagaagaggtgttccagggcttggtttccttgaggaagttacatattcttcaatgcaagaatctgacaggactcacacaagctcgtgggcaatctacactagcaccaagtgaactcctgccacgtttggagtccctgcagatacgccgttgttattcttttgtagaggtccccaacctaccgacgtctctcaagctattacaaattacggactgccatgacctgaggtccatcatattcaatcagcagcaggatacgacgatgttggtgagtgcagaaagcttcgcacagccggacaagtcatcgttaatatcagggtccaccagcgagaccaacgatcgcgtccttccacgcctagaatctctagtaatagagtattgcaatcgtttgaaggttctccatcttcctccgtccatcaagaaattggatattgtacgctgtgaaaaacttcagtcactctcaggaaagctggatgccgttcgagcattaaatatcagctattgcgggagcttgaaatcactggaatcttgcttaggagagctcccgtcgctgcaacacctcagacttgttaattgcccaggcctggtatccttgccgaaggggcctcaagcatactcatctcttacatctcttgaaattcgttattgttctggtataaatttgcttccaccgagcctgcagcaacgtctggatgacatcgagaataaagaactagatgcctgctatgaagaatcagaggcaaaaccagaaccaaagtctcctcatcgtcaatctgcaatcagtaggctgctgtgcttggagtag</cdnaseq> |
| Protein Sequence |
<aaseq>MAELMATMVVGPLLSMVKDKASSYLLEQYKVMEGMEEQHEILKR KLPAILDVIADAEEQAAKHREGAKAWLEELRKVAYQANDVFDEFKYEALRRKAKAKGH YKKLGSIVVIKLIPTHNRILFRYRMGNKLRMILNAIEVLIAEMNAFRFKFRPEPPMSS IKWRKTDSKISNLSMDIANKSRKKDKEEIVNRLLAQASNGDLTVIPIVGMGGMGKTTL AQLVYNDPEIQKHFQLLLWLCVSDNFDVDSLAKRIVEAAPKEMNKKNDNGGAKKLPQD ELKEVVSGQRYLLILDDVWNRDASKWEALKYNLKHGGSGSSVLTTTRDQAVAQLMAPA QEVYDLKNLNESFIEEIIKRSAFNSEQERPPPELLEMVGDIAKKCSGSPLAATALGST LRTKTTKKEWDAILSRSTICDEENGILPILKLSYNCLPSYMRQCFSFCAIFPKDHEID VEMLIQLWMANGFIPEKQGECPEIIGKRIFSELVSRSFFQDVKGIPFEFHDIKCSKIT CKIHDLMHDVAQSSMGKECATIATELSKSDDFPYSARHLFFSGVIFLKKVYPGIQTLI CSSQEELIRSSREISKYSSLRALKMGGDSFLKPKYLHHLRYLDLSYSKIEALPEDISI LYHLQTLNLSICDCLCQLPNGMKYMTALRHLYTHGCWRLKSMPPDLGHLTCLQTLTCF VAGSCSGCSDLGELRQLDLGGRLELRKLENVTKADAKAANLGKKEKLTELSLRWTGQK YKEAQSNNHKEVLEGLTPHEGLKVLSILHCGSSTCPTWMNKLRDMVKLVLDGCKNLEK LPPLWQLPALEVLCLEGLDGLNCLFNCDIYTSFTFCRLKELTLASMRNFETWWDTNEV KGEELIFPEVEKLIIKSCPRLTALPKASNVISELSGGVSTVCHSAFPALKEMELYGLD IFQKWEAVDGTPREEVTFPQLYKLDIRRCPELTTLPEAPKLRDLNIYEVNQQISLQAA SRYITSLSSLHLHLSTDDTETAPVAKQQDSSELVIEDEKWNHKSPLELMDLTGCNLLF SYPSALALWTCFVQLLDLNIWEVDALVDWPEEVFQGLVSLRKLHILQCKNLTGLTQAR GQSTLAPSELLPRLESLQIRRCYSFVEVPNLPTSLKLLQITDCHDLRSIIFNQQQDTT MLVSAESFAQPDKSSLISGSTSETNDRVLPRLESLVIEYCNRLKVLHLPPSIKKLDIV RCEKLQSLSGKLDAVRALNISYCGSLKSLESCLGELPSLQHLRLVNCPGLVSLPKGPQ AYSSLTSLEIRYCSGINLLPPSLQQRLDDIENKELDACYEESEAKPEPKSPHRQSAIS RLLCLE</aaseq> |
| Gene Sequence |
<dnaseqindica>5191..5264#517..4423#acgggctacagcggcgagtgacgcgcttgcggctgcaggctgctgagctcgtccgtcaagcgccgccttaatttgagacgggtccgagcagctccaaccttgctctccacgaaccggccaagcccaattcgccgttgagttccggtcgacggaggaacacaagaacgacagggcaccacaccggtgagcatcccagcttcttctcagctatcgtcagtacttcgtggttgcgcttgcgccgaccgaccggcttctctcccgcgcactccagttttctatctctatcgtcgcccagttctcggaagcttactgcactgtactgtacttgcttacttcttcattccaatcaaacactttcattccaggcagcaactcttaatactagctagttgcattcagttaaacacattccttcagtttccatttcccacaaaaagcagctgctgctcacactccttgcttattgtgcttccctgtgttctgttcgtgtgttcttagctctaggagatccttccgatggcagagctgatggccaccatggtggtcgggccactgctgtccatggtgaaggacaaggcctccagctacctcctggagcagtacaaggtcatggagggcatggaggagcagcatgagattctcaaacgcaagctgcccgccatcctcgacgtcatcgccgacgccgaggagcaggcggcaaaacacagggaaggggcgaaagcatggcttgaggagctccggaaggtggcctaccaggccaatgatgtcttcgacgagttcaagtacgaggcactccgccgcaaagccaaggccaaggggcactacaagaagctcggcagcattgttgttataaagctcattcctactcacaaccgtattctgtttcgttataggatgggcaacaagctcaggatgatcctgaatgccattgaagtcctaattgcagagatgaatgcctttaggtttaagttccgaccagagccaccaatgtcctccatcaaatggagaaagacggattctaaaatctccaacctttctatggacattgccaacaagtcaagaaagaaagacaaagaggagattgtcaatcgattgcttgctcaagccagcaatggggatctcactgttattcccattgtaggaatgggggggatgggcaagaccaccttagcgcagctcgtttacaatgaccctgaaattcagaagcattttcagttgctgctctggttgtgtgtctctgacaacttcgatgtggattcgctggcaaaaagaatagttgaagcagctcccaaagaaatgaataagaagaatgataatggaggggccaaaaagttaccacaggatgaacttaaagaagttgtgagtgggcagaggtacctcctcattttggatgatgtctggaaccgtgatgccagtaagtgggaagcactcaagtacaaccttaagcatggtggcagcggtagctcagttttgacaacaactcgtgatcaagcagttgctcaactaatggctccagctcaagaagtttatgatctcaagaacctgaatgaaagcttcattgaggaaattatcaagagaagtgctttcaattcagaacaagaaaggcctcctcctgagctactcgaaatggttggtgatattgccaagaaatgttctggttcccctttagctgcaacagcattgggctctacactgcgtacgaagaccaccaagaaagaatgggacgctatattgagcagaagcacaatttgcgatgaggaaaatggaattttaccaatactcaaactcagttacaattgcttgccatcatatatgcggcaatgtttttccttctgtgctatttttcccaaggatcatgagatcgacgtggaaatgctgatccagttatggatggccaatggttttatcccggagaaacaaggagagtgccctgaaatcattggtaaaagaattttcagtgagttggtgtcaaggtcattttttcaggatgtgaaggggatcccatttgagttccatgatataaagtgctctaagattacttgtaagatccatgaccttatgcatgatgttgcacaatcttccatgggaaaagaatgcgctactatagctacagaacttagtaaaagtgatgattttccttattccgctcgccacttgtttttctcaggtgtcattttcctaaagaaagtatatccaggtatccaaacattgatatgttcctcgcaagaagaattgatacgttcctcgcgagaaatatcaaaatacagttcattgcgagctttaaagatggggggagattcattcctgaaaccaaaatatcttcatcacctgaggtatcttgatttgtcatacagtaaaattgaagcacttcctgaagacataagcatcctatatcatctgcaaacattgaacctttccatttgtgactgtctttgtcaactcccaaacggaatgaagtacatgaccgccctccgtcacttgtacactcacggatgttggaggttaaaaagcatgcctccggacctcggacacctcacttgcctacagacgcttacatgttttgtagccggttcttgctctggctgcagtgatttgggagagctgcggcagttggaccttggtggtcgactagagctaagaaaactggaaaatgtgacaaaagctgatgcaaaagcagcaaatctcggaaagaaggaaaaactgaccgaattgagcttaagatggactggtcagaagtacaaggaggcacagagtaataatcataaagaggtgctggaaggtctcacgcctcacgaggggctcaaggttctgagtatacttcactgtgggagcagtacatgtccaacatggatgaataaactgcgggacatggtgaagcttgtgttagatggttgcaaaaatctcgagaagcttcctccactgtggcagctgccagctctagaagttctttgccttgaaggactggatggtttaaattgcttgttcaactgtgacatatacacctcctttacattttgcagactgaaggagcttaccttggccagtatgagaaattttgagacatggtgggacacaaatgaggtaaaaggagaagagctgatatttcctgaggttgaaaagctgataatcaaaagttgtcctaggctaactgccttaccaaaagcgtcaaatgtgatttcagaattgtctggcggagttagcaccgtgtgtcattctgcatttccagcattgaaggaaatggaattatatggtttggatatctttcagaaatgggaggcagtcgatggaactccaagggaggaggtaacatttcctcagctttacaaattagatattagacggtgcccagagttgactactctacctgaagcaccaaagctgagagacttaaacatatatgaagtcaatcagcaaatatccctacaggcagccagcagatatattacttcattgtccagtctccacctgcatttgtcaactgatgacacagaaacagcaccggtggccaagcaacaagattcgagtgaattggtgattgaggatgagaaatggaatcataaatctcccctggaacttatggacttaactggctgcaaccttttattctcttacccaagtgcgttggctctgtggacatgttttgttcagctcctagatctgaatatttgggaagtcgatgcgcttgtcgactggccagaagaggtgttccagggcttggtttccttgaggaagttacatattcttcaatgcaagaatctgacaggactcacacaagctcgtgggcaatctacactagcaccaagtgaactcctgccacgtttggagtccctgcagatacgccgttgttattcttttgtagaggtccccaacctaccgacgtctctcaagctattacaaattacggactgccatgacctgaggtccatcatattcaatcagcagcaggatacgacgatgttggtgagtgcagaaagcttcgcacagccggacaagtcatcgttaatatcagggtccaccagcgagaccaacgatcgcgtccttccacgcctagaatctctagtaatagagtattgcaatcgtttgaaggttctccatcttcctccgtccatcaagaaattggatattgtacgctgtgaaaaacttcagtcactctcaggaaagctggatgccgttcgagcattaaatatcagctattgcgggagcttgaaatcactggaatcttgcttaggagagctcccgtcgctgcaacacctcagacttgttaattgcccaggcctggtatccttgccgaaggggcctcaagcatactcatctcttacatctcttgaaattcgttattgttctggtataaatttgcttccaccgagcctgcagcaacgtctggatgacatcgagaataaagaactagatgcctgctatgaaggtaatcttcagtttcttaaccgtgtacgatttagtggtaaaagtttcgagcgtggtgtctagaacccctagtcaaccattaatatgattatttgtacatagagtacaatgcgcattcataactcacttcttcagctgtgtcatctaaaccctttaaactttgagttgcatttgggtatctaatcgcatacaaaggaatttagtaatatctccagtagctattccttatatatgatgatctcttcctgtgattatgcttgctagtttggactatgtaattaatttttgccgggtcgactatgtaattaattgacttcatttagtcgccaagtgtggcatcatgcaattattatggcaaagctgtattagtcatgatcgaagccacttggtgaacttattcccttctactttgaaccaatactcattgatcatttccctttaagcgtttgatatggacaacactttaaattcgcagagctaactaacgcagcgcttgtctttacatttctttcttttctatatggtatataagggtagaatggtaatttcattcataccgctgcatgcgaggatgacttgacatactcatgtggcatttttgtgggtccaaggactatattaacccaaataattaatttgaaggacttgtttggacgatttgaaacatcgatgactaaattgagcccgaagagaaacttcaggagtcgcattggatattcacccctatatattcaatacatataaattttcttttcttttgtttgccagaatcagaggcaaaaccagaaccaaagtctcctcatcgtcaatctgcaatcagtaggctgctgtgcttggagtagcagtttcaggaccaaatgagagttgtcatcatggtttgtaacgcgtgcttgatgattgatttcgaatgaaattctagccactatccttatgcttactctctctatggtgtgacttccttgtaggatcgttaccgaatctgcaattgttgggttattaattgtgtgattgtatctcatggtagaatgtattacctacctaccttttctcaagataaactccactaaagtcgatcttctgtttggtgttgttgagttgtggtccaaatttatttgcacttaataaatgccagcttctgccgtagcggagcggaggcccgtcgtcgggaggcttgggctggagcgtcgccgggaggcctgcccgtcatcccttttagagttccaccatatatatacctcttgtaagccgccttacggcgatgtaacctcacctcagatatagtaaagatattttgctggctggc</dnaseqindica> |
| External Link(s) |
