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| | ==Structured Information== | | ==Structured Information== |
| − | {{JaponicaGene|
| |
| − | GeneName = Os09g0451500|
| |
| − | Description = Thioredoxin domain 2 containing protein|
| |
| − | Version = NM_001069866.1 GI:115479474 GeneID:4347226|
| |
| − | Length = 4864 bp|
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| − | Definition = Oryza sativa Japonica Group Os09g0451500, complete gene.|
| |
| − | Source = Oryza sativa Japonica Group
| |
| | | | |
| − | ORGANISM Oryza sativa Japonica Group
| |
| − | Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
| |
| − | Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
| |
| − | clade; Ehrhartoideae; Oryzeae; Oryza.
| |
| − | |
| |
| − | Chromosome = [[:category:Japonica Chromosome 9|Chromosome 9]]|
| |
| − | AP = Chromosome 9:17584752..17589615|
| |
| − | CDS = 17584851..17584976,17585080..17585238,17586351..17586464,17587063..17587244,17587504..17587603<br>,17588180..17588356,17588446..17588588,17588992..17589141,17589224..17589398<br>|
| |
| − | GCID = <gbrowseImage1>
| |
| − | name=NC_008402:17584752..17589615
| |
| − | source=RiceChromosome09
| |
| − | preset=GeneLocation
| |
| − | </gbrowseImage1>|
| |
| − | GSID = <gbrowseImage2>
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| − | name=NC_008402:17584752..17589615
| |
| − | source=RiceChromosome09
| |
| − | preset=GeneLocation
| |
| − | </gbrowseImage2>|
| |
| − | CDNA = <cdnaseq>atgcgccccgcggtcgccgccgccctcctcctcgtcgccgccgccgtcgcggcctcgccggtttccgccctctactccgccggctctcccgtcctccagttcaaccccaacaacttcaaatccaaggtgctgaactcgaatggggtggtgctggtggagttcttcgcgccgtggtgcgggcactgccagcagctgacgccgatttgggagaaggccgcgggcgtcctcaagggcgtcgccacggtcgccgcgctcgacgccgacgcgcacaaggagctcgcgcaggaatatggaataagaggatttccaactattaaggtgtttgttcctggaaagcctccagtagattatcaaggagcaagagatgtaaagccaattgttgaatttgctctttcacaggtcaaggctcttcttagagatcggttgaatggaaagacatcagcaggttcaggtggcaagaaatcaggtggttcaagtgagaaaactgaaccaagtgcgtcaatagaattaaattctcaaaacttcgataaacttgtcaccaaaagcaaggatctttggattgttgagttctttgcaccatggtgtgggcactgcaagaaattggcacctgaatggaaaaaggctgcaaagaacttgaagggccaagtgaagctaggtcatgtggattgtgatgctgaaaagtctttgatgagcaagtacaaggtagaaggctttcctactattttggtatttggtgccgataaggagagcccattcccttaccagggggctagagttgcctctgctatcgagtcctttgcattggagcagttggaagccaatgctgctccacctgaagtttctgagttgactggcccagacgccatggaagagaaatgtgcttctgctgccatttgctttgtatctttccttccagatatcttggattcaaaggccgaaggaagaaacaagtaccttgagctgctattatctgttgctgagaaatttaaaaagagtccatacagttttgtctggacagctgctgggaagcaagctgatcttgagaagcaagttggagttggtggttatggctatccagcgatggttgctctcaacgtgaaaaaaggcgcatatgctccactccgtagtgctttccagcttgatgaaataactgagtttgtgaaggaagcagggcgcggtgggaagggtaatcttcctttggacggtaccccaacgatagtccaatctgaaccatgggacggcaaagatggtgaggttattgaagaggacgaattctcccttgaggagctgatggccgacaactctccagtaaatgacgagttgtga</cdnaseq>|
| |
| − | AA = <aaseq>MRPAVAAALLLVAAAVAASPVSALYSAGSPVLQFNPNNFKSKVL NSNGVVLVEFFAPWCGHCQQLTPIWEKAAGVLKGVATVAALDADAHKELAQEYGIRGF PTIKVFVPGKPPVDYQGARDVKPIVEFALSQVKALLRDRLNGKTSAGSGGKKSGGSSE KTEPSASIELNSQNFDKLVTKSKDLWIVEFFAPWCGHCKKLAPEWKKAAKNLKGQVKL GHVDCDAEKSLMSKYKVEGFPTILVFGADKESPFPYQGARVASAIESFALEQLEANAA PPEVSELTGPDAMEEKCASAAICFVSFLPDILDSKAEGRNKYLELLLSVAEKFKKSPY SFVWTAAGKQADLEKQVGVGGYGYPAMVALNVKKGAYAPLRSAFQLDEITEFVKEAGR GGKGNLPLDGTPTIVQSEPWDGKDGEVIEEDEFSLEELMADNSPVNDEL</aaseq>|
| |
| − | DNA = <dnaseqindica>100..225#329..487#1600..1713#2312..2493#2753..2852#3429..3605#3695..3837#4241..4390#4473..4647#cctcgcgagcgaacttaaaatcgcagtcgcaccccggcgccgatcgcaaggtccacctcctcccctctccactccttctagaaccttctcgaccccgccatgcgccccgcggtcgccgccgccctcctcctcgtcgccgccgccgtcgcggcctcgccggtttccgccctctactccgccggctctcccgtcctccagttcaaccccaacaacttcaaatccaaggtctctcgatcgactcccccctcacactcttcccttccgtctccggatcggcttcccttacttgggggctctcgctgagttcgaatttcggttgggggtgcaggtgctgaactcgaatggggtggtgctggtggagttcttcgcgccgtggtgcgggcactgccagcagctgacgccgatttgggagaaggccgcgggcgtcctcaagggcgtcgccacggtcgccgcgctcgacgccgacgcgcacaaggagctcgcgcaggtgagcaaacgtcctgtttggcatctcggttcattgggattggctgatctaccttttcctggtgtgatctgatctctgcatcagttgttgcgtcaacgtaatttgatcgagctaggacataactgcgtatatgcaatgtgcaaactcgactccataaaaaaaaaagttacacctgttatgggttttttgtgtagctctcagtatcagagagtacaatgccacatttgtttggtggaggaaatatgggaagatcaatgtttggtgtctacacgtactgatcaaaaaggggcgtcgtttttattttttaaaggagggcattaacatcggatatgagttatacaataccgaattaatgcatattaaaatgctgtagctgggattaatattgatggaatgtggtgcattgttgatttcatacagtaaatttatttattttttcactttttcagcatggtgattggatgctgttctcttttttttaaaaaaaataacctggattttgaattatattgaacgaattaagcattttaattggtgttaattagggttttatctgtttttcttttgatccgaaggtgagagcggccttggttagtttattgccaataaaagtgtatatgtgactttagatgtgggaaatgcatacttttatgaaggacgaaaccgtggaagccaatgggttgtgcaactttacaatatctgatgcaacattgtttattggtgaatcagaaaattaggttatgtatcccagattctctaactgaaaatttagtcactcatcaaatgaaataaaggcatctatcctgagagaaggcaagcaagagtgaaagatagttatgtacctttgccattgtacctgcagtgcggtgattttgagtctctgtcatatttagacatatatttttttttacagtaattttacagtatcatgcttattgaccaagaggcattttttagctacgttatatctttgttttggtgagtattagtcttctgtctaggctaaatcaataaaaactgcacattagaacgtcttccaccattgccatttgtaaatattttgactggatgctatcaagtttttcctgatgcatgatgcctttgttctgcaggaatatggaataagaggatttccaactattaaggtgtttgttcctggaaagcctccagtagattatcaaggagcaagagatgtaaagccaattgttgaatttgctctttcacaggtttgttgcagcttgccctatttgatatatataaaaatgatatgttgtttttacttttattgctcctgttctatctgccactccactagtgtatacaactacaaatccttttctgaagatgttaagtgcttcctggattttcacagaccaaacttattcagattgcaattgtcagctgaatgttgacataaataacttttatcttaacatgaagtattactaggggggaaaaacaacagatatcatatatcaactgatactaatgtcctaggtagctaattgattttgctatatatctccctcaaatgctaaacctttcaaacttatcaactttttatatgggatactggatatgaggtgatgcagtgatgcttctcagttcttaccatggtggagctttatgtacctgctcttgcacagctggtgcttctggtagcacccatgattttcgttaaaaaatacactgtctgtcgctgctgtgttacagtgggttgccattttaccttttttttttgggtatttctctgtaaaaaatttcagccttgtgacacacgatatgagtttccacttttaaatacaatgaatctgtatcctacaggtcaaggctcttcttagagatcggttgaatggaaagacatcagcaggttcaggtggcaagaaatcaggtggttcaagtgagaaaactgaaccaagtgcgtcaatagaattaaattctcaaaacttcgataaacttgtcaccaaaagcaaggatctttggattgttgagttctttgcaccatggtatgtggcttaatgcatttatggttttcatgttgactgatttattctttactgttgtttctgaacttaactctgaatccaaaagtagagaaggaaagtgaagaaaaacacaaaatttcatacaatttagtcacatgttagtattgtagtgtgtacattttagatcattgcccttaagattatgttgttcaagataataagtaaatcctgttaactgaagacattatttgactcattaactgtactggtcttttgtaaggtgtgggcactgcaagaaattggcacctgaatggaaaaaggctgcaaagaacttgaagggccaagtgaagctaggtcatgtggattgtgatgctgaaaaggttcttgccctttttctatttggtcataagtttttgttgtatgtgcatttcttgtttgcaaataatttatacaaaattataaactgaaatttgacaatgcattggatatttcatcaattcaaaggactttgataaaggtattgtaaaataaaattaaactgagggttagcactctagtttatagggacacaatcaggatatgcataggaaagtacaaaatgctgtatcaaggaaagcaatggcctaactttatcctaaggcatttgttgggatcttattttgtgctgttttaggttgtctctttaacagtttaacatgatgtactatgattgaagttttatgtttttttttctagaatactaattcaagtgtttggtcagttgataaaagttaccgagtgagtttaaatgaacttaggcagaaaattcaggcagtgtatcagtatatttataaaacacagttaatgtttgacctttttaaagcattcatgtgcgttctgtacaacatttgtcaaactatacaacctagttttcaagtaccacttatgtaacataacgtgtgacttgaattttgcagtctttgatgagcaagtacaaggtagaaggctttcctactattttggtatttggtgccgataaggagagcccattcccttaccagggggctagagttgcctctgctatcgagtcctttgcattggagcagttggaagccaatgctgctccacctgaagtttctgagttgactggcccagtaagttttcgtgatattaacaaagttatccttttgtggagacaatggtctgggattgatgttttgacgaggtggtttggcttctgtaggacgccatggaagagaaatgtgcttctgctgccatttgctttgtatctttccttccagatatcttggattcaaaggccgaaggaagaaacaagtaccttgagctgctattatctgttgctgagaaatttaaaaagagtccatacaggcaagattcaactaaacttgttgtggactttataccattctacctatcattccttgtcttgttatgtcttttgagataaaaaaatccacagttaaattgatacattaatttccttttgctattgaatattgattgacaaaaagattgatagtaaccttcatatatttgaaacaccttaaaagaattaaatgacttagttcgataatgttatatgccaaggcgtgtatactataagctctaaacataatggagattttatatattatatatgcaaatctgccatgtgactgagttctatgttgagaagtttcctcagacaaatccattcacttagttaatgccttgaaatctcttccgtttcttgagaaagccattctgagctagaaaaagcattgtcttgcagttttgtctggacagctgctgggaagcaagctgatcttgagaagcaagttggagttggtggttatggctatccagcgatggttgctctcaacgtgaaaaaaggcgcatatgctccactccgtagtgctttccagcttgatgaaataacgtaagttccttgtgcatagaaaattaattgctgcttaaggttcatttgccagatgtttgataacaaaaccggtctcctgcagtgagtttgtgaaggaagcagggcgcggtgggaagggtaatcttcctttggacggtaccccaacgatagtccaatctgaaccatgggacggcaaagatggtgaggttattgaagaggacgaattctcccttgaggagctgatggccgacaactctccagtaaatgacgagttgtgattctgataattccggaaatgatgttagagttgattagccagactgtaaatctgagtaacactaatgcttcactcgtgtcaactgtacaatttgggcgggacaattttcagaatgctcctttggtgttattacagtacattttagttgaagaaaaacatcgtttgtggtcttttaggagggatgcttttctctgggaaataaaactacttcgtaatct</dnaseqindica>|
| |
| − | Link = [http://www.ncbi.nlm.nih.gov/nuccore/NM_001069866.1 RefSeq:Os09g0451500]|
| |
| − | }}
| |
| | [[Category:Genes]] | | [[Category:Genes]] |
| | [[Category:Japonica mRNA]] | | [[Category:Japonica mRNA]] |
Latest revision as of 09:44, 12 June 2015
Please input one-sentence summary here.
Annotated Information
Function
Protein disulfide isomerase (PDI), a ubiquitous sulfhydryl oxidoreductase found in all eukaryotic cells, is multifunctional enzyme, which catalyzes a wide range of thiol-disulfide exchange reactions, including oxidation, reduction, and isomerization, and also displays chaperone activity.
PDIL2;3 and PDIL1;1 are not functionally redundant in sulfhydryl oxidations of structurally diverse storage proteins and play distinct roles in PB development. PDIL2;3-dependent and PDIL2;3-independent oxidation pathways that sustain disulfide bonds of crP10 in PB-I.
Expression
Complementation analyses using PDIL1;1 knockout esp2 mutant indicated that the a and a' TRX domains of PDIL1;1 exhibited similar redox activities and that PDIL2;3 was unable to perform the PDIL1;1 functions. PDIL2;3 knockdown inhibited the accumulation of Cys-rich 10-kD prolamin (crP10) in the core of PB-I. Conversely, crP10 knockdown dispersed PDIL2;3 into the ER lumen. Glutathione S-transferase-PDIL2;3 formed a stable tetramer when it was expressed in Escherichia coli, and the recombinant PDIL2;3 tetramer facilitated α-globulin(C79F) mutant protein to form nonnative intermolecular disulfide bonds in vitro.
Evolution
Protein disulfide isomerase (PDI) family oxidoreductase PDIL2;3, an ortholog of human P5, contains a conserved structural disulfide in the redox-inactive thioredoxin-like (TRX) domain and was efficiently targeted to the surface of PB-I in a redox active site-dependent manner, whereas PDIL1;1, an ortholog of human PDI, was localized in the ER lumen.
Labs working on this gene
Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan
Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan
Faculty of Life Science, Yamaguchi Prefectural University, Sakurabatake, Yamaguchi 753-8502, Japan
References
Yayoi Onda;Ai Nagamine;Mutsumi Sakurai;Toshihiro Kumamaru;Masahiro Ogawa;Yasushi Kawagoe
Distinct Roles of Protein Disulfide Isomerase and P5 Sulfhydryl Oxidoreductases in Multiple Pathways for Oxidation of Structurally Diverse Storage Proteins in Rice
The Plant Cell, 2011, 23(1): 210-223
Structured Information
